RosBREED Demonstration Breeding Programs

Jim Luby, Breeding Team Leader, Univ. of Minnesota Outline of Presentation  Intro to Demonstration Breeding Programs and Traits for each RosBREED Crop  Strawberry  Peach  Tart Cherry  Sweet Cherry  Apple

 Reference Germplasm Sets Tart and Sweet Cherries • Tart Cherry - Amy Iezzoni - Michigan State University • Sweet Cherry - Nnadozie Oraguzie - Washington State University RosBREED Cherry Phenotyping

• Sweet Cherries for Fresh Market in Pacific Northwest with firmness and a range of colors • Tart Cherries for Processing in Michigan – Fruit size genes to enable leaf spot introgression from wild relative – Freestone for better pitting Strawberry

• Chad Finn - USDA-ARS, Corvallis, OR • Jim Hancock - Michigan State University • Tom Davis - University of New Hampshire • Phil Stewart - Driscoll Strawberry Associates RosBREED Strawberry Phenotyping

• Entire germplasm set evaluated at multiple locations • Allows for dissection of GxE effects in PBA • Includes fresh market and processing fruit traits • Plant architecture and remontancy (rebloom) Peach

• Ksenija Gasic - Clemson University • Tom Gradziel – UC-Davis • John Clark - University of Arkansas • Dave Byrne - Texas A&M University RosBREED Peach Phenotyping

• Covers southern and western geography of US peach production, range of chilling zones • Several market types – Fresh, Processing – Nectarine, acidity/sugar, flesh color, shape • Disease resistances (bacterial spot, brown rot) Apple • Kate Evans - Washington State Univ. • Susan Brown - Cornell University • Jim Luby - University of Minnesota RosBREED Apple Phenotyping

• Primary emphasis on fresh market traits

• Components of Flavor, Texture and Appearance

• Postharvest life and storage disorders Traits and Standardized Phenotyping Protocols • Identify critical fruit quality traits and other important traits

• Develop standardized phenotyping protocols to enable data pooling across locations/institutions

• Protocols available at www.RosBREED.org • “Fruit Evaluation” Reference Germplasm Sets Crop Reference Set (CR Set) Breeding Pedigree Set (BP Set) Crop Reference Set (CR Set) • ~480 individuals (cultivars, ancestors, founders, breeding lines, selections, and seedlings) that are fruiting in 2010-2012

• Enable efficient validation and utility assessment of Marker-Locus-Trait associations

• Genotyped genome-wide with SNP markers and phenotyped for fruit quality traits and other high- impact traits.

• Resource for common benefit Breeding Pedigree Sets (BP Set)

• ~100-300 extra plants for each breeding program that, together with the CR Set, allow full representation of important parents in that program • Information may remain proprietary with each breeding program; data-sharing improves power Crop Reference Set Visualizing Structure in a Breeding Program with PediMapTM Tart Cherry Crop Reference Set: Visualizing a Breeding Program • Up to 5 generations

• Important ancestors: – English Morello – Meteor

• Pedigree linkage Tart Cherry Crop Reference Set: Visualizing a Breeding Program 39 individuals Half are selections

4 crosses containing 201 individuals provide representation of important parents

20 Ancestors unavailable Crop Reference Set Allele Representation Strategy for Constructing Reference Germplasm Sets  Pedigree-linked reference germplasm sets represent alleles across the genomes of Important Breeding Parents

 Each relative represents an Important Parent; goal was

>12.5 representation units (e.g. 25 F1 s) for statistical power • Offspring represent alleles of these Parents • Other relatives can also represent Parent alleles through shared ancestors (because a proportion of alleles will be Identical By Descent)

 F1 offspring, full-sibs, and parents provide best genome coverage Sweet Cherry Crop Reference Set

• Up to 6 generations P8-79 BlackRepublican 47-4 Selah Bing Rainier EmperorEugenie Pop_SelahxVan 46-11 EmperorFrancis • 49 individuals Benton 7903-2 Van Stella Napoleon Glacier Pop_7903-2xSumm – 38 cultivars 8007-2 Windsor Lambert Cashmere – 11 selections Summit Kordia Chelan Sweetheart Pop_8007-2xChln JI • 18 crosses Bertoille Newstar Beaulieu Pop_DDxMIM17 Corum containing 191 GG Sam Pop_Swthtx8007 Eugenie Pop_RainxPMR1 Pop_LapxBrooks individuals (5 to 24 Regina GilPeck BB PMR-1 per cross) DD Pop_GGxSwtht Pop_LapxTieton Katalin EarlyBurlat MIM17 Margit StMargaret Pop_RainxBent Lapins Lapins Pop_SwthtxBB NY54 Pop_BingxPMR1 BlackEagle Brooks Rube Pop_LapxChel Tieton Pop_SwthtxReg • „Lapins‟ is an Schmidt Pop_PMR1xVan Pop_KordiaxUNK Ambrunes Pop_LapxAmb Schneiders Important Parent Pop_RainxReg Representation by offspring in CRS

Lapins represents itself 1 1.0 Lapins No. trees

16 * 0.5 21 * 0.5 10 * 0.5

Selection A Selection B Population 1 Population 2 Population 3 Population 4

1 1 14 13 8 10

Direct descendants of Lapins each

Pedigree diagrams are provide 0.5 units allele representation outputs of Pedimap program Representation by other relatives

BlackHeart Napoleon Emperor Francis Immediate ancestors of 0.5 Lapins

Lambert JI2420 EmpressEugenie

0.5

Stella Van 0.5 cv Emperor Francis represents Lapins at 0.125 units (0.5 x 0.5 x 0.5 x 1 Lapins individual)

Selection A Selection B Population 1 Population 2 Population 3 Population 4 Representation by other relatives

BlackHeart Napoleon Emperor Francis

0.5 0.5

Lambert JI2420 EmpressEugenie Population 5

0.5 24

Population 5 represents Stella Van Lapins at 1.5 units 0.5 (0.5 x 0.5 x 0.5 x 0.5 x 24 offspring)

Lapins

Selection A Selection B Population 1 Population 2 Population 3 Population 4 Total representation of Lapins in the sweet cherry CR Set 7 units

Van and relatives Stella and relatives

12 units 11 units

Lapins is represented at 58 units in the sweet cherry Crop Lapins Reference Set (16 units)

27 units

Direct descendants Crop Reference Set Integrating Multiple Breeding Programs Apple Peach Strawberry Apple Crop Reference Set • Need to integrate 3 breeding programs • Much overlap of Important Parents among 3 breeding programs • Maximum 6 generations • Results in CR Set with much commonality and high pedigree connectedness • Allows transfer of information among breeding programs NY59333-006

Empire Blackjon McIntosh 669NJ5 NY70712-53

NY55158-002 NY88 RussianSeedling Delicious AutumnCrisp Jonathan Coop-18 Crandall AurGoldGa NorthSpy Fuji PacQueen MN1708 NY752 PacRose RomBeauty Honeycrisp P14-126 Arlet MN1628 NY03 Monroe NY10 EsopSpitz Nicola Keepsake

Melrose WSU2 Sweet16 NY02 BC8S-27-43 Frostbite

Clivia Pinova BC-8S-27-2 Pop_AurxCP Chinook GoldenDel KidsOrRed Gala Zestar U of MN Braeburn Pop_AurxEnt Apple Crop DuchOlden Splendour Sansa CrippsPink GranSmith Enterprise Cox Pop_AurxArl Sundowner Rubin Idared NY08 LadyWill PRI1661-1 Vanda Pop_AurxHC 35 Important StateFair PRI1661-2 NY632

YelTransp Reference Set Empress WSU49 Wagener MN1691

Burgundy Malinda WSU50 Mantet Akane Silken WSU52 Oriole Lambourne Parents Wealthy Sonya

Fireside Scifresh Jonamac WSU57 BenDavis Goodland WSU24 VistaBella NY241 WSU37 AE0251 Montgomery WorcPearm Honeygold NY04 JamesGr BlushiGol AE0206 Sunrise NY05

Melba NY07

Coop-17 EarlyCortland WSU30 NY01 CornellPop08 Haralson

NY06 NY11 Linda Cortland WSU17 WSU46 NY92607-16 Lodi NY247 WSU36 Delorgue

Hatsuaki Delblush Fiesta NY14 NY66305-289 Jonagold CornellPop13 Wijcik MN1915

NY543 F2_26829-2-2 MN1942 Suncrisp Topaz MN1955 MN1702 GingerGold Williams MN2034 WSU35 MN1789 Beacon MN1965 Raritan MN2062 Liberty Macoun WSU48 MN1971 Hudson Spartan MN1764 MN1980 NY15 MN2016 MN2036 MN1802 Regent NY65707-19

MN1984 Pop_EntxArl MN1627 Freedom Monark MN2056 RedBaron Jonafree MN2057 Pop_HCxAr PitmPine Goldrush MN2063

Snowsweet WSU34 Prima Mflor821 MN2000 Britemac WSU65 GMAL4332 NY13 MN1706 NJ90 MN1937 IngMarie CornellPop09 MN1988 MN1940 Winesap Coop-15 Dolgo MN2031

GMAL4329 Fantazja WSU38 WintBanana MN2042

AE_HCxJonf NY76406-007 Fortune

YellNewtown AE0118 AE0406 CoromandelRed MN1839 Pop_HCxCP MN1760 BeautBath AE0213 Pop_HCxEnt MN1914

Cameo MN1899 MN1925 GMAL4327 CornellPop07 NY12 Ambrosia MN1964 MN2033 Elstar GMAL4328 PRI14-126 MN1837 MN2041 MN1888 Kerr CrimsCrisp Sundance MN2044 MN1836 Sawa Tsugaru NY913 MN2050 Dayton NY09 NJ53 WSU7

MN2046 PixieCrunch Discovery WSU5 Redfree

AE0210 NY59333-006

Empire Blackjon McIntosh 669NJ5 NY70712-53

NY55158-002 NY88 RussianSeedling Delicious AutumnCrisp Jonathan Coop-18 Crandall AurGoldGa NorthSpy Fuji PacQueen MN1708 NY752 PacRose RomBeauty Honeycrisp P14-126 Arlet MN1628 NY03 Monroe NY10 EsopSpitz Nicola Keepsake

Melrose WSU2 Sweet16 NY02 BC8S-27-43 Frostbite

Clivia Pinova BC-8S-27-2 Pop_AurxCP Chinook GoldenDel KidsOrRed Gala Zestar Wash. State Braeburn Pop_AurxEnt Apple Crop DuchOlden Splendour Sansa CrippsPink GranSmith Enterprise Cox Pop_AurxArl Sundowner Rubin Idared NY08 LadyWill PRI1661-1 Vanda Pop_AurxHC 38 Important StateFair PRI1661-2 NY632 Reference Set YelTransp Wagener MN1691 Empress WSU49

Burgundy Malinda WSU50 Mantet Akane Silken WSU52 Oriole Lambourne Parents Wealthy Sonya

Fireside Scifresh Jonamac WSU57 BenDavis Goodland WSU24 VistaBella NY241 WSU37 AE0251 Montgomery WorcPearm Honeygold NY04 JamesGr BlushiGol AE0206 Sunrise NY05

Melba NY07

Coop-17 EarlyCortland WSU30 NY01 CornellPop08 Haralson

NY06 NY11 Linda Cortland WSU17 WSU46 NY92607-16 Lodi NY247 WSU36 Delorgue

Hatsuaki Delblush Fiesta NY14 NY66305-289 Jonagold CornellPop13 Wijcik MN1915

NY543 F2_26829-2-2 MN1942 Suncrisp Topaz MN1955 MN1702 GingerGold Williams MN2034 WSU35 MN1789 Beacon MN1965 Raritan MN2062 Liberty Macoun WSU48 MN1971 Hudson Spartan MN1764 MN1980 NY15 MN2016 MN2036 MN1802 Regent NY65707-19

MN1984 Pop_EntxArl MN1627 Freedom Monark MN2056 RedBaron Jonafree MN2057 Pop_HCxAr PitmPine Goldrush MN2063

Snowsweet WSU34 Prima Mflor821 MN2000 Britemac WSU65 GMAL4332 NY13 MN1706 NJ90 MN1937 IngMarie CornellPop09 MN1988 MN1940 Winesap Coop-15 Dolgo MN2031

GMAL4329 Fantazja WSU38 WintBanana MN2042

AE_HCxJonf NY76406-007 Fortune

YellNewtown AE0118 AE0406 CoromandelRed MN1839 Pop_HCxCP MN1760 BeautBath AE0213 Pop_HCxEnt MN1914

Cameo MN1899 MN1925 GMAL4327 CornellPop07 NY12 Ambrosia MN1964 MN2033 Elstar GMAL4328 PRI14-126 MN1837 MN2041 MN1888 Kerr CrimsCrisp Sundance MN2044 MN1836 Sawa Tsugaru NY913 MN2050 Dayton NY09 NJ53 WSU7

MN2046 PixieCrunch Discovery WSU5 Redfree

AE0210 NY59333-006

Empire Blackjon McIntosh 669NJ5 NY70712-53

NY55158-002 NY88 RussianSeedling Delicious AutumnCrisp Jonathan Coop-18 Crandall AurGoldGa NorthSpy Fuji PacQueen MN1708 NY752 PacRose RomBeauty Honeycrisp P14-126 Arlet MN1628 NY03 Monroe NY10 EsopSpitz Nicola Keepsake

Melrose WSU2 Sweet16 NY02 BC8S-27-43 Frostbite

Clivia Pinova BC-8S-27-2 Pop_AurxCP Chinook GoldenDel KidsOrRed Gala Zestar

Braeburn Pop_AurxEnt Apple Crop DuchOlden Splendour Sansa CrippsPink GranSmith Enterprise Cornell Cox Pop_AurxArl Sundowner Rubin Idared NY08 LadyWill PRI1661-1 Vanda Pop_AurxHC StateFair PRI1661-2 NY632

YelTransp Reference Set Empress WSU49 Wagener MN1691 18 Important Burgundy Malinda WSU50 Mantet Akane Silken WSU52 Oriole Lambourne Wealthy Sonya

Fireside Scifresh Jonamac WSU57 BenDavis Parents Goodland WSU24 VistaBella NY241 WSU37 AE0251 Montgomery WorcPearm Honeygold NY04 JamesGr BlushiGol AE0206 Sunrise NY05

Melba NY07

Coop-17 EarlyCortland WSU30 NY01 CornellPop08 Haralson

NY06 NY11 Linda Cortland WSU17 WSU46 NY92607-16 Lodi NY247 WSU36 Delorgue

Hatsuaki Delblush Fiesta NY14 NY66305-289 Jonagold CornellPop13 Wijcik MN1915

NY543 F2_26829-2-2 MN1942 Suncrisp Topaz MN1955 MN1702 GingerGold Williams MN2034 WSU35 MN1789 Beacon MN1965 Raritan MN2062 Liberty Macoun WSU48 MN1971 Hudson Spartan MN1764 MN1980 NY15 MN2016 MN2036 MN1802 Regent NY65707-19

MN1984 Pop_EntxArl MN1627 Freedom Monark MN2056 RedBaron Jonafree MN2057 Pop_HCxAr PitmPine Goldrush MN2063

Snowsweet WSU34 Prima Mflor821 MN2000 Britemac WSU65 GMAL4332 NY13 MN1706 NJ90 MN1937 IngMarie CornellPop09 MN1988 MN1940 Winesap Coop-15 Dolgo MN2031

GMAL4329 Fantazja WSU38 WintBanana MN2042

AE_HCxJonf NY76406-007 Fortune

YellNewtown AE0118 AE0406 CoromandelRed MN1839 Pop_HCxCP MN1760 BeautBath AE0213 Pop_HCxEnt MN1914

Cameo MN1899 MN1925 GMAL4327 CornellPop07 NY12 Ambrosia MN1964 MN2033 Elstar GMAL4328 PRI14-126 MN1837 MN2041 MN1888 Kerr CrimsCrisp Sundance MN2044 MN1836 Sawa Tsugaru NY913 MN2050 Dayton NY09 NJ53 WSU7

MN2046 PixieCrunch Discovery WSU5 Redfree

AE0210 NY59333-006

Empire Blackjon McIntosh 669NJ5 NY70712-53

NY55158-002 NY88 RussianSeedling Delicious AutumnCrisp Jonathan Coop-18 Crandall AurGoldGa NorthSpy Fuji PacQueen MN1708 NY752 PacRose RomBeauty Honeycrisp P14-126 Arlet MN1628 NY03 Monroe NY10 EsopSpitz Nicola Keepsake

Melrose WSU2 Sweet16 NY02 BC8S-27-43 Frostbite

Clivia Pinova BC-8S-27-2 Pop_AurxCP Chinook GoldenDel KidsOrRed Gala Zestar

Braeburn Pop_AurxEnt Apple Crop DuchOlden Splendour Sansa CrippsPink GranSmith Enterprise Cox Pop_AurxArl Sundowner Rubin Idared NY08 All Programs‟ LadyWill PRI1661-1 Vanda Pop_AurxHC StateFair PRI1661-2 NY632

YelTransp Reference Set Empress WSU49 Wagener MN1691

Burgundy Malinda WSU50 Mantet Akane Silken 72 Important WSU52 Oriole Lambourne Wealthy Sonya

Fireside Scifresh Jonamac WSU57 BenDavis Goodland WSU24 VistaBella NY241 AE0251 Montgomery WSU37 Parents WorcPearm Honeygold NY04 JamesGr BlushiGol AE0206 Sunrise NY05

Melba NY07

Coop-17 EarlyCortland WSU30 NY01 CornellPop08 Haralson

NY06 NY11 Linda Cortland WSU17 WSU46 NY92607-16 Lodi NY247 WSU36 Delorgue

Hatsuaki Delblush Fiesta NY14 NY66305-289 Jonagold CornellPop13 Wijcik MN1915

NY543 F2_26829-2-2 MN1942 Suncrisp Topaz MN1955 MN1702 GingerGold Williams MN2034 WSU35 MN1789 Beacon MN1965 Raritan MN2062 Liberty Macoun WSU48 MN1971 Hudson Spartan MN1764 MN1980 NY15 MN2016 MN2036 MN1802 Regent NY65707-19

MN1984 Pop_EntxArl MN1627 Freedom Monark MN2056 RedBaron Jonafree MN2057 Pop_HCxAr PitmPine Goldrush MN2063

Snowsweet WSU34 Prima Mflor821 MN2000 Britemac WSU65 GMAL4332 NY13 MN1706 NJ90 MN1937 IngMarie CornellPop09 MN1988 MN1940 Winesap Coop-15 Dolgo MN2031

GMAL4329 Fantazja WSU38 WintBanana MN2042

AE_HCxJonf NY76406-007 Fortune

YellNewtown AE0118 AE0406 CoromandelRed MN1839 Pop_HCxCP MN1760 BeautBath AE0213 Pop_HCxEnt MN1914

Cameo MN1899 MN1925 GMAL4327 CornellPop07 NY12 Ambrosia MN1964 MN2033 Elstar GMAL4328 PRI14-126 MN1837 MN2041 MN1888 Kerr CrimsCrisp Sundance MN2044 MN1836 Sawa Tsugaru NY913 MN2050 Dayton NY09 NJ53 WSU7

MN2046 PixieCrunch Discovery WSU5 Redfree

AE0210 Peach Crop Reference Set

JH_Hale

O'Henry Fairhaven • 4 Breeding programs Flordaprince Halehaven Frank OP11 TropicBeauty Redhaven Pallas

Bolinha Candor Clayton • Limited overlap of germplasm P34-106 P97-14 CAF4 Nemaguard Saturn Loring ChineseCling between market types BY03P22388 Greensboro Redskin Everts OP1 CAF2 Goldprince TX2293-3 Andross A34-160 P.argentea S-37OPxNemaguard Hakuho UFGold White_County Georgia_Belle ArkC Winblo Blazeprince • 118 cultivars and selections Rizzi Y142-75 CAF3 Orange_Cling Lola TX-Pop2 2001,7-180 ZinDai Springtime BY01P6160 Mayfire TXW1293-1 91,17-262 F8,5-166 ArkB Contender BY01P6245 SC-Pop1 Dr._Davis

BY00P6346-t SC-POP22 • 373 progeny in 23 crosses Jefferson Guardian Peento Dixon1 18,8-11

18,8-23 SC-Pop2 St_John Lovell TX2B136 China-Pearl Nonpareil Cumberland OP12 Marigold Diamante Early_Crawford SC-POP_Blush • Up to 11 generations Hiley Mayflower Springold CA-Pop2 RioOsoGem S-37 SC-POP_G TX-Pop3 Springbrite Admiral_Dewey Okinawa SC-POP09 OP10 Riegels SC-Pop3 OP13 OP14 Crimsonbaby Panamint Slappey Summercrest TX-Pop1 CA-Pop1 Kakamas OldmixonFree AR-Pop1 CA-Pop3 Babcock LeGrand F10C,12-28 SC-POP23 Sunfre Peach Crop Reference Set Complete Pedigree

NJ5107397 Panamint PanaxJewel FLAQ3033 TX2B136 Jewel Sunlite FLA15-85W Candoka Columbina Hermosillo FLA2-13 NJN21 FLA3-4N Sunfre

Palomar Kaygold P42-81 FLA4-65 FLA68-50 FV131-48 P42-91 Okinawa Sunhigh JH_Hale Springbrite P97-14 Southland Early_Crawford Elberta FLA5-5 TX2293-3 FLA8B27 ChineseCling Goldprince OP43 TXW1293-1 FLA35-30 Fairhaven OP36 FLA10-48 K61-48 P60-38 VP EarlyCrawford Halehaven TropicBeauty Y142-75 NJ53939 FLA2-7 P83-48 P87-147

OP19 Southhaven G69-83N FLA16-61 FLA3-56 P87-79 FV89-14 FV3-257 FLAR9T10 Springtime Sunrise P37-79 Babcock Robin Earligold Springcrest D33-1 Armking Carman NJ40CS Springold Slappey NJN55 SunGrand P34-106 NJ291 dummy2 FLA3-2 SprGoldxSunfre K6E121 PalxSprtime St_John FV15-48 OP29 VP CrimsonLady Luken's_Honey R1T6 VP Sungrand NJF8

July_Elberta Saturn NJ271 Luken'shoneyxJElberta Flordaprince Strawberry Arp_Beauty Golden_Globe KimxJuly_Elberta 561483 2000,2-18 Peento Fireglow BY01P6160 Mayflower A34-160 StrawberryxPeento D2-46-143-- K519-47 FLA5-58 Cumberland 104325 602903 Crimson_Lady OP10 RR38-4-662001 Sunred LeGrand Maravilha CA-Pop-9-33-341 P57-56 113115 FLA28-48 FV317-97 F8,5-166 UFGold Marigold Mayfire Admiral_Dewey O'Henry Frank OP35 Goldfinch Dix,22A-5 OP13 Kim VP F85-147 Meredith CA-Pop-7-25-178 Red_Diamond OP4 JHHxQuetta Armqueen 554039 FV9-46 Lola Dix,8 Valiant 2000,A-235 OP44 Nectared4 Hiley Nectared7 Merrill.Bon NJ230 FLA9-20C CA-Pop-6-1-3 TardyxJordan JHHxLippiatt 555036 Quetta P51-138 2000,3-157 P107-78 Lippiatt RR105-10-- 2000,8-138 Levi Early_Hale Hakuho 2000,8-149 Loring Babygold7 OP38 F8,5-147 2000,8-150 JHHxLevi 554367 CA-Pop-6-1-33 OP24 Crimsonbaby F8,5-156 2000,8-153 125936 OP23 Jungerman Dix,6-6 F8,5-161 2000,8-154 P30-122 Y150-13 NJN14 5110301 2000,8-157 Jerseyland F8,5-162 Stock3-17 NJ66325 562025 F8,5-164 2000,8-158 5109028 E22-59 CA-Pop-7-26-80 Pallas 76925 C1-25-177-- 2000,8-164 Dix,5A-1 F8,5-165 OP21 5102896 F8,5-171 DukeofGA Georgia_Belle TuscanxPaloro Prarie_Dawn 13232xCherryred F8,5-172 K173-9 582990 2000,A-241 (TxP) C1-19-122-- F8,5-179 CA-Pop-7-10-235 OP2 RR51-32-- F8,72-33 F8,5-182 CA-Pop-7-11-23 OP28 562158 2000,16-93 OP11 FLA92-2C 5110007 NJ196 90,10-91 OP22 58127 RR71-322-- OP26 Loadel Da30-13 2001,18-215 NJ257 2000,16-125 CA-Pop-8-35-81 OP12 5106543 OP32 Cherryred Red_Grand Paloro Summercrest OP41 A130P 2000,16-110 Lemon_Free Dixon1 7-5 Raritan_Rose VP 90,10-92 Contender P83-62 P48-57 CAF2 Hesse CA-Pop-8-42-50 PI35201 Dixon2 2000,16-177 OP48 89,10-44 13232 FLAK5W35 OP37 89,10-53 2000,2-15 94727 Dix,58-6 Fortuna BY96P3240 OP46 89,10-55 KimxLeGrand Early_Sun_Grand SC-POP22 PI91459 89,10-59 CA-Pop-8-55-50 BY01P6245 PI101681 89,10-60 NJ156 NCA2219 Dix,16-3 Honeydew_Hale 89,10-61 38026 Ark_392 Candor OP40 FLNCseedling FLA8-11 Ark_433N China-Pearl Splendid CA-Pop-7-3-47 NonpareilBF Wolvamade VP DrDavis Bolivian 98838 TardyNonpareil 6128 OP45 A270N BY00P6346-t Dr._Davis OP39 F8,76-45 91,18-6 96,1-171 CA-Pop-7-9-28 OP31 54P455 91,17-195 NJLA3 White_County Greensboro Everts Walgant VP Woltemade DukeofYork 18,6-33 99,4-123 Reina_Elena TachibanaWasa Goodwin CA-Pop-9-34-69 5105965 NCA2679 CA-Pop-7-2-167 88,2-75 Riegels Hann_Almond F10C,12-28 2000,12-110 NC7366 OP47 FLA84-18C 2000,16-199 P79-56 2000,12-56 Pekin 98,9-7 Mexican_Honey VP Dixon NC64 2000,16-202 96,9-292 BY81P2840 OP25 CA-Pop-8-17-71 Kalhaven VP Vilmos Lovell OrangeClingSdlg 88,2-77 RR122-15 AustralianMuir 98,4-177 2000,8-37 Orange_Cling Blazeprince 2000,8-69 F10C,20-51 Ark_145N E22-59N Transvaal-Cling Redskin 2000,8-71 CA-Pop-8-19-90 S-37OPxNemaguard Redhaven GoodmansChoice 18,9-41 96,9-171 Alameda 87,13-13 2000,A-151 RoundTuscan 2005,10-10-90 92,10-119 87,12-31 Ark_224 D25-9E Y140-77 P91-23 CAF3 TX-Pop3 Nonpareil CA-Pop-8-25-99 NC7130 89,9-82 Jordanolo 90,9-116 2000,2-8 CA-Pop-5-1-78 2000,A-219 GoldenGlory 90,9-134 2000,A-220 2000,2-9 F8,1-121 93,5-220 91,17-262 Bonanza Lilliland GH2-48 93,5-24 90,10-120 OP17 G40-5E CA-Pop-8-27-128 93,5-26 E7-35 91,9-161 CA-Pop-5-4-228 2000,16-207 dummy1 Leader_sdlg 93,5-50 Clayton Andross Hakuto 97,2-19 93,3-108 93,1-75 CA-Pop-9-34-301 Denjuro Winblo 93,3-22 CA-Pop-8-30-54 CA-Pop-5-5-248 OP7 Lilleland 93,3-225 Y142-194 92,10-125 Diamante 2000,8-44 CA-Pop-7-4-176 ArkC 92,14-73 93,3-247 2000,16-159 OP15 Yellow_Alberge Fla.86-28C Norman CA-Pop-10-1-5 CA-Pop-7-3-157 Erly-Red-Fre CA-Pop-5-9-248 93,5-80 2000,16-209 90,1-4 97,5-270 2000,16-92 OP16 93,3-159 Oro_A 2001,7-126 OP30 93,4-166 CA-Pop-5-9-76 ArkB PI,292557 CA-Pop-10-3-5 VP F85-166 93,4-185 CA-Pop-7-7-146 18,8-11 96,9-8 VP HoneydewHale 96,4-49 2002,1-79 OP5 CA-Pop-9-1-198 2000,10-223 96,4-51 CA-Pop1 R3-53 96,4-55 Rizzi CA-Pop-8-61-26 CA-Pop-5-21-145 Ross A419-P 93,3-175 CA-Pop-8-33-208 94,4-223 R1-1 CA-Pop-7-3-204 CA-Pop-8-5-100 2000,1-215 93,3-189 Tuscan 2000,1-222 CA-Pop-6-4-234 Leader Carson 96,5-11 CA-Pop-7-13-72 H,6-55 2000,1-228 2002,15-255 OP27 96,7-165 2000,1-234 CA-Pop-7-6-82 ZinDai CA-Pop-9-22-229 CA-Pop-9-34-227 2000,B-217 99,12-155 Fla.79-20C VP D,62-193 CA-Pop-7-11-108 CA-Pop-7-18-192 2000,B-218 OP8 2001,19-6 F8,1-42 2000,B-219 OP9 CA-Pop-9-34-253 VP Padre_al P7-34 P78-49 TX-Pop2 F8,1-96 CA-Pop-9-5-273 CA-Pop-5-5-86 PI134401 LateRoss CA-Pop-7-11-24 CA-Pop-5-22-204 Bolinha CA-Pop-6-5-95 2005,22-166 SC-Pop1 CA-Pop-10-2-10 dummy93 2000,3-159 Bolinha3 CA-Pop-7-5-207 2005,23-6 Guardian dummy94 E,1-16 CA-Pop-7-13-130 Bolinha6 CA-Pop-7-17-214 dummy92 F10E,1-16 CA-Pop-9-12-102 Bolinha7 H_523 CA-Pop-7-8-68 CA-Pop-9-1-265 CA-Pop-5-19-168 CA-Pop-5-17-24 Jefferson CA-Pop-9-14-48 97,9-200 Ark_154N CA-Pop-7-26-67 CA-Pop-9-23-152 Kakamas CA-Pop-8-15-77 97,9-273 CA-Pop-8-41-92 OP3 89,6-103 CA-Pop-9-20-1 CA-Pop-5-19-79 98,8-188 RedGold CA-Pop-5-2-200 CA-Pop-7-1-1 99,16-131 CA-Pop-9-23-25 2W_68W 2000,16-133 CA-Pop-7-13-63 CA-Pop-9-20-174 CA-Pop-6-4-51 RR_53-194 CA-Pop-7-17-29 Jing CA-Pop-9-5-82 91,12-4 CA-Pop-9-28-284 CA-Pop-9-9-250 OP14 CA-Pop-5-17-192 CA-Pop-5-20-139 2000,15-116 CA-Pop-9-7-274 91,13-26 CAF4 TX-Pop1 BY03P22388 OP6 CA-Pop-7-13-245 2000,15-119 CA-Pop-9-31-182 91,13-7 CA-Pop-7-19-164 D62-193 2000,15-122 96,1-148 CA-Pop-9-30-233 CA-Pop-8-9-165 CA-Pop-7-23-78 OP1 2000,15-126 96,1-149 CA-Pop-8-13-154 CA-Pop-9-7-91 Halford 2000,15-127 CA-Pop2 CA-Pop-8-4-112 2003,1-329 CA-Pop-9-31-99 Ogawa CA-Pop-9-31-301 CA-Pop-7-19-20 2002,6-283 2000,15-73 CA-Pop-9-4-49 CA-Pop-5-18-246 19,1-40 2000,15-88 CA-Pop-9-7-244 CA-Pop-7-9-218 StHelena 2000,3-205 CA-Pop-9-14-25 CA-Pop-9-33-66 dummy98 CA-Pop-8-18-11 CA-Pop-9-32-253 CA-Pop-5-18-11 CA-Pop-7-27-1 2005,10-223 P.mira#19 S-37OP 2001,1-185 2005,10-228 Klamt CA-Pop-9-34-105 VP CA-Pop-8-38-106 F8,1-71 Da,30-13 2001,7-180 2005,10-236 2001,2-4 CA-Pop-8-42-67 P.scoparia 2000,3-217 97,5-152 2005,11-56 93,5-19 CA-Pop-7-2-73 P.argentea 98,4-24 CA-Pop-5-10-61 CA-Pop-5-12-199 18,8-23 2001,2-84 2005,12-231 2001,3-303 CA-Pop-8-27-83 TX86606 OldmixonFree CA-Pop-5-9-231 2005,12-256 CA-Pop-7-13-254 CA-Pop-10-1-7 P55-454 CA-Pop-10-2-8 CA-Pop-8-33-7 CA-Pop-8-47-55 S-37 2001,2-93 CA-Pop-5-7-168 RioOsoGem CA-Pop-9-22-282 dummy89 2000,15-241 Nemaguard CA-Pop-9-17-197 CA-Pop-10-1-9 CA-Pop-8-20-64 2002,9-76 dummy90 CA-Pop-5-18-181 CA-Pop-8-52-11 CA-Pop-8-4-99 99,19-23 CA-Pop-9-36-29 dummy91 CA-Pop-7-10-3 CA-Pop-5-8-229 2000,B-202 D6-15W 2002,9-8 18,9-8 CA-Pop-10-1-11 SC-POP_Blush CarolynG Nickels CA-Pop-9-23-55 CA-Pop-5-11-227 CA-Pop-7-2-209 Klampt 2005,12-51 SC-Pop3 89,15-309 CA-Pop-7-10-198 9,20-C 2000,11-177 2001,9-130 persicaXdavidiana Brights 2001,9-215 91,12-54 2000,11-58 CA-Pop-10-2-6 Yumyeong CA-Pop-9-20-126 SC-Pop2 92,7-25 CA-Pop-7-16-218 2001,9-65 OldmixonCling 2004,2-142 CA-Pop-7-17-1 dummy97 Hansen1 2001,7-256 99,15-99 CA-Pop-9-30-298 LateCrawford CA-Pop-5-1-223 2000,11-157 2002,3-236 dummy99 CA-Pop-10-1-3 SC-POP09 Hansen2 F8,5-104-146 2004,2-148 VP KimxJelberta CA-Pop-5-17-147 2000,8-64 CA-Pop-5-12-88 Merrill_Prince 98,2-132 OP20 2001,9-275 2002,3-124 F8,5-55 Dix22A-5 2001,9-305 CA-Pop-5-14-124 SC-POP23 CA-Pop-8-3-157 2002,3-128 CA-Pop-8-28-264 CA-Pop-5-16-146 dummy96 2004,2-150 F8,72-22 2001,9-6 CA-Pop-5-16-209 OP18 Ross*R,1-1 PP904 CA-Pop-9-1-150 CA-Pop-9-20-301

dummy95 2002,1-50 CA-Pop-9-24-286 AR-Pop1 2000,6-38 SC-POP_G CA-Pop3 99,15-145 OP33 CA-Pop-5-9-33 FLA4695 CA-Pop-7-12-126 2002,13-152 OP34 OP42 Crop Reference and Breeding Pedigree Sets Allele Representation of Important Parents in Multiple Breeding Programs Allele Representation of 72 Important Parents in Apple Germplasm Set

CRS +UMN BPS 35 Parents

6 6 CRS + Cornell BPS 18 Parents 13 10

8 CRS +WSU BPS 38 Parents Deficient (<12.5 units) 10 Adequate (12.5-20 units) 6 Very good (21-50 units) 15 Excellent (>50 units) 17 Strawberry Large Integrated Crop Reference and Breeding Pedigree Set Strawberry Crop Reference Set • 26 generations • Covers several germplasm groups - CA, PNW, Eastern US, Europe, Wild • 209 cultivars and selections • 78 wild strawberries - F. virginiana, F. chiloensis or F. virginiana x chiloensis • 618 seedlings in 44 crosses • Results in CRS with much commonality and high pedigree connectedness Strawberry Crop Reference Set

Hudson_Bay

dummy13

OSC_346 White_Carolina OSC_1765 Keens_Seedling Totem Puget_Beauty Methven_Scarlet Korona dummy15 Nanaimo ORUS_850-4 OSC_3746 dummy14 Sparkle Northwest OSC_2732 Charles_Downing Independence Tamella OSC_2975 dummy10 Hovey Wilson ORUS_850-48 Gorella ORUS_750-1

Narcissa OSC_2414 dummy7 Keens_Imperial Brightmore Juspa Linn Neunan dummy17 F.chiloensis(Chile) Induka OSC_3845 ORUS_1076-124 OSC_2931 Puget_Summer F.virg'Native_Iowa' OSC_2012 F.chiloensis(Ecuador) OSC_456 Cal_52.16-13 ORUS_746-4 dummy6 OSC_2136 NJ_1051 Downer's_Prolific Molalla ORUS_2781-1 dummy5 F.chiloensis(PatPoint) OSC_2242 OSC_3727 Puget_Reliance Ross_Phoenix Crescent Royal_Sovereign Cal_52.16-15 OSC_4727 dummy11 US_3763 OSC_1509 NC__1768 WA_87010-7 F.chiloensis(Peru) Siletz King_of_the_Earlies

Wiltguard ORUS_1069-3 F.chiloensis US_543 NC_1759 Cal_5649-1 N.J.Scarlet(F.virg) F.chiloensis(Rockaway#3) Redgauntlet Vale WSU_1945 OSC_1757 Benton Belmont Haverland ORUS_2016-1 NC_1767 F.chiloensis(ScottsCreek) Talisman Goliath Hoffman OSC_1816 Douglas Cal_64.57-108 MDUS_3082 Jucunda Sharpless Green_Prolific OSC_2467 NW_90049-19 F.vesca F.chiloensis10-B OSC_4681 US_634 NC_1768 NW_90054-36 dummy2 Excelsior US_3374 US_4127 MDUS_3184 Cal_829.9 FRA_1075 Ruby BC_77-2-72 Laguna OP OSC_4682

F.virginiana-M_Marshall FRA_1088 Surecrop Cal_611.4 Cal_39.117-4 Marshall Laxton's_Noble Fairland Muir ORUS_2493-2 Dorsett MDUS_1972 F_Marshal Lassenxself

FRA_1092 MDUS_430 Anaheim Miner Cal_70.3-117 Ettersburg_121 Jessie Cal_51.51-1 Redcrest Selva Likely_Lake_Stevens Howard_17 FRA_1100 Temple Sierra Lassen WSU_1985 ORUS_984-49 Irvine Aberdeen Likely_Chesapeake OSC_4357 Fairfax Cal_161.1 FRA_1104 WSU_1697 Missionary OSC_2234 Valley_Red OSC_1490 William_Belt Cal_103.22 FRA_1108 Cumberland_Triumph Cal_42.8-16 Bountiful Cal_69.62-103 OSC_2100 OSC_2315 Cal_83.25-2 dummy1 Portia OSC_1491 OSC_3624 Cal_65.63-601 FRA_1267 Huachi(Ambato_NAH) Chesapeake OSC_154 Sequoia

Jersey_Queen Cal_468.4 Seascape FRA_1691 OSC_305 Cal_A.08 Cal_168.1 OSC_3628 Cheam dummy9 Tillamook BC_41-14R-5 Parry ORUS_3304 FRA_1692 Noble Suwannee Tribute TD8 Cal_168.3 Seedling#4Howard OSC_821 Cal_186.1 Cal_588.1 Markee ORUS_767-4 FRA_24 Cal_65.65-601 OSC_2981 USEB_18 UnserFritz OSC_3551 NJ_selection (SharplessxParry)F3 Oregon Cuesta Konig.von.Sachsen FRA_34 Cal_39.96-18 OSC_4445 Fairpeake Cal_177.19 F_Konig.von.Sachsen Sieger Tenn_Beauty Albritton ORUS_1267-236 Fern dummy16 OSC_3554 FRA_357 Redstar Clyde Cal_177.21 Bubach Cal_54.124-2 NC_2967 FRA_368 Captain_Jack Fendall ORUS_1267-250 OSC_3577 Tufts Cal_71.98-604 ORUS_2180-1 Blakemore MDUS_683 US_4152 Cal_474.2 Earlibelle Scot_BK-46 FRA_372 ORUS_869-13 Souvenir_de_Charles_Machiroux Cal_39.82-19 Fendalcino Cal_63.7-101 Cal_71.98-605 FRA_42 Cal_21.9 F_Fendall Cal_159.1 Nich_Ohmer WSU_685 MDUS_2611 Parker ORUS_3305 OSC_7 Tioga Cal_58.45-11 Middlefield NW_90005-1 FRA_48 Pinnacle dummy8 Sunrise Cal_86.6 NC__1053 MDUS_3364 EarliglowxChandler FRA_688 US_3328 Columbia F.virg.glauca.Wasatch ORUS_923-316 Cal_BH_14_(Mixed_Cross) Midland FRA_743 MAR1A Cal_53.10-2 Cal_59.41-1 WSU_1623 Claribel OSC_1629 ORUS_2427-1 MDUS_2101 Rainier WA_80053-17 OSC_3653 US_836 OSC_2475 FRA_796 WSU_1149 WA_7531-22 ORUS_1723-3 Cal_370.14 Shuksan Cal_59.41-14 Cyclone(Old) Cal_43.1-36 Cal_60.19-2 ORUS_2161-1 Stelemaster OSC_812 Evita MDUS_4258 Cal_85.92-602 Rose_Ettersburg US_253_OP Yaquina_B OSC_2769 ORUS_1722-2 Rockhill_Derivative Cal_752.23 Redgem OSC_2950 Cal_37.20-45 WSU_157 WSU_1574 ORUS_1009-1 dummy4 BRA1A Laxtons_Noble F.virginiana Del_Norte Seedling#1Howard Senga_Sengana Cal_7.20 Cal_752.31 ORUS_3321 F1_hybrid Olympus F.chil.Cape.Mendicino WSU_175 OSC_4319 Streamliner Cal_7.9a Earliglow Cal_35.102-5 ORUS_2427-4 Oso_Grande Cal_72.361-105 Early_Jersey_Giant Eurisko Cal_46.5-1 Cal_720 OSC_3596 Firecracker Sarian Montana_Missionary Pawtuckaway Cal_564.6 OSC_6058 Dunlap S0_20-2 OSC_2853 Van_Deman Cal_403.8 Elsanta ORUS_2075-1 Fred9xLH50-4 Cal_86.5 Cal_564.7 Cal_55.32-1 MN_1181 Yellow_Wonder US_3358 Shasta B144 Joe Bemidji_Chief OSC_767 Tyee Alpine(F.vesca) ORUS_1735-1 MSU_56 Glendale Cal_68.24 Cal_337.2 OSC_4863 ORUS_1027-1 Cal_460.11 Ark_5063 OSC_3044 MDUS_5132 WA_88097-47 Cal_68.25 Cal_337.3 S.65122 ORUS_1376-16 NY_113 Auchincruive_Climax Solana Gandy ORUS_3324 Cal_77.3-603 FL_83-418 RH30xMR10 Oberschlesien Delite Cal_135.18 Cal_249.5 FL_84-1389 Cal_59.51-11 Kaiser_Samling OSC_3991 Clark NY_844 Fort_Laramie OSC_4275 FL_84-3163 (Providence_self) US_253 Camarosa B753 FL_83-1452 Cal_179.15 Cal_222.11

((MFruherxSSengana)xGeorgSolt) K87-5 Harvester F.virginiana(AL) MDUS_2713 Pajaro Cal_179.2 B754 Cal_222.4 OSC_2066 Cardinal Formans_Excelsior Aiko Chandler F.virginiana(MS) RH30xLH50-4 SCXMAR1A CF1116 Pocomoke ORUS_1384-3 Kent Klondike Cal_67.5 WSU_1532 Early_Ozark F.virginiana(NC) Geneva Honeoye Capitola Donner FL_87-418 K86-19 Cal_75.121-101 Ark_5431 F.virginiana(SC) OSC_3040 Cal_Z_11 NY_4626 MN_2374 Cal_10.21 OSC_18 Earlidawn Pan_American Redheart OSC_2338 Brighton ORUS_3325 NJ_8713-8 FRA_110 Jewel OSC_2122 K72-9 LA_59-2012 SCxFRA24 NAHxBRA1A FL_87-200 Cabot FRA_1435 NC__1065 Redglow Macherauchs_Marieva Cavendish LA_59-138 OSC_2261 ORUS_1091-1 Darselect Cal_Z_9 FL_80-456 FRA_1455 ((KlonmxMidl)x(TennShipxMidl)) MDUS_4463 Massey Everest F.moschata NY_254 Cassandra FRA_1466 OSC_2371 Princesse_Dagmar K79-5 Southland (GorellaxMDUS3184) WIUS_8008 Glooscap K68-58 Tiogax(Redgauntx(WiltgxGorel)) T._de_Leopold FRA_1694 Tenn_Shipper Raritan MR10xRH23 SCxNAH OSC_1815 Providence Reiko OSC_59 Marlate FRA_1695 MSU_9-13 Vanguard Cal_145.38a Kurume103 WSU_828 MDUS_4377 OSC_1776 Joliette NJ_821-5 FRA_1696 MDUS_2188 ArKing HM1xNAH ORUS_3277 FavourxNY844 NJ_459 Prinz_Julius_Ernst Holiday K74-5 MDUS_4380 K79-4 FRA_1697 WSU_438 ORUS_1391-1 Hummi_Gento Tangi (Tenn_Shipper_x_Midland) Pathfinder MR10xN8688 Strawberry_Festival Uberreich FRA_1698 WSU_450 Bounty MDUS_4386 Dixieland Dana MSU_49 NJ_357 Micmac MSU_9-2 Sel.Salvi_N.15

FRA_1699 Cal_145.52 Mesabi US_542 Pocahontas Florida_90 Cal_144.2 (WiltguardxGorella) Sitkahybrid FRA_1700 FVC_18-1 K61-87 WSU_14 MDUS_2650 Governor_Simcoe Cal_144.21 Annapolis 46-4J Cal_66.96-101 BelrubixMacherauchs_Marieva Jerseybelle FRA_1701 Ottawa485 Lulu FVC_11-1 Hogyoku Capron Macherauchs_Fruhernte ORUS_1083-135 MSU_9-6 J.23 FVC_18-18 PocahontasXRegina FRA_1702 Deutsch_Evern Cal_32.50 WA_94023-1 SJ85189 (Klonmore_x_Midland) Beaver Gariguette Progressive FRA_1703 Cal_32.63 Regina OSC_3965 Scott ORUS_3278 Berri_Supreme ORUS_1827-2 (MachFruherntexSengana)

F.chiloensis-M_Sitka F.cuneifolius FRA_338 FVC_11-2 MDUS_2359 (Redgauntx(WiltgxGorella)) MDUS_3700 Aptos Cal_66.2 F_Sitka Belrubi FRA_58 Benizuru SYS_3672-179 ORUS_3307 Corvallis NY_1221 MDUS_4616 BC_91-14-31 US_4144 FVC_30-1 WIUS_8002 NW_90055-35 Red_Giant FRA_982 NY_316 F.virginiana_ovalis Valentine Rockhill Hood F_JH_101-1 FVC_11-3 NY_E58 WSU_2833 Lion's_Head_3 Guardsman Guardian Dover J._Riffaud FVC_30-2 Red_Rich Favour RedgauntletxKorona FL_69-1302 Madame_Lebreton MS_34-12 Redchief (Sitkahybridx46-4J) Miyazaki Redrich Vibrant Etna ORUS_3323 ORUS_1407-76 MDUS_2386 OSC_4867 MSU_9-11 MS_34-4 FVC_11-4 The_Sun Redcoat FVC_30-3 Duluth Sweet_Charlie MSU_9-15 ORUS_3279 Midway Hapil F_ORUS_1407-76 Sable_Beach_8 OSC_913 K88-4 Sumas

B6.117 Sitka Cal_43.2 Lateglow y32b Georg_Soltwedel? MNUS_99 D5.23 FVC_30-4 OSC_1366 FVC_11-5 MDUS_2966 FL_71-189 G7 Ostara ORUS_2262-2 ORUS_2361-1 Harunoka ORUS_1769-1 G8 NYUS_119 Lupton US_3919 General_Chanzy Ettersburg_80 MDUS_3816 NJ_953 L1 MDUS_2927 MSU_9-16 FVC_30-5 Rabunda BC6 Hansa

Rosa_Linda FVC_11-6 Macherauchs_Dauerernte MSU_9-14 H4 NJ_925 WSU_1651 ORUS_986-129 Glenheart Cal_814.6 Whonnock (Miyazaki_x_The_Sun) Cal_604.4 Stolo Palmyre Hummi_GentoxOstara ORUS_1754-1 Ettersburg_20 Cal_491.1 Chilean Repita Ville_de_Paris Yellow_WonderXPawtuckaway Klonmore ORUS_3314 British_Queen ORUS_2490-1 MDUS_2992 MDUS_3694 ((GorellaxMDUS3184)xTioga) Cal_87.112-6 Crown FVC_8-1 NETHBIN MSU_9-7 Veestar ORUS_2240-1 Cal_134.31 Rotkappchen MDUS_2321 Cal_88.270-1 E-30324 Florida_Belle Cal_94.16-1 MDUS_2905 Cal_213.4 ORUS_1963-5 FVC_33-1 Rockhill_selfed FL_82-1556P FVC_8-2 US_2506 US_4419 Joe_Reiter ((Sitkahybridx46-4J)xOttawa485) MNUS_52 Cal_470.9 MDUS_4587 FRA_1557 MSU_9-17 LA_2556 ORUS_2676-1 Atlas LA_883 Cal_378.16 Cal_490.9 US_2267 ORUS_973-1 Washington ORUS_3308 General_de_Castelnau FVC_8-3 Gem Cal_42.17-18 LB48 OSC_4474 L'Acadie St._Joseph MSU_9-12 Cheyenne3 NC_2839 Ada_Herzberg ORUS_2159-2 Lucie_Boiselet JH_101-1 NC_2665 Pickerproof ORUS_3315 ORUS_3319 Everglade FRA_1408 FVC_8-4 Twentieth_Century ORUS_3306 (MidlandxCheyenne2) J92D12 Duc_de_Malakoff FVC_10-1 NC_1729 Cal_648.2 Shuswap OSC_4816 Fukuba MSU_9-9 BCPink Easypicker (RockhillxCheyenne3) ORUS_3326

British_Sovereign FRA_1414 FVC_8-5 Protem MSU_9-4 (FairfaxXF.ovalis) OSC_4817 E-30397 Cal_794.19 ORUS_3316 ORUS_3317 Cal_45.5-1 Bismarck MSU_9-1 Diamante Commander ORUS_2742-1 dummy12 FVC_10-2 Cal_37.2-11 Cal_543.14 Allstar OSC_4916 Cheyenne2 ORUS_740-7 dummy18 Aromas Cal_800.13 Trumpeter LB48xH4 ORUS_3320 dummy3 ORUS_3318 Revada Cal_638.45 Frel Cal_74.3 ORUS_2369-1 Burgundy FVC_17-20 FRA_1620 Hecker Apollo MSU_9-5 Mara_des_Bois FRENCHBIN MSU_9-10 Docteur_Morere LA_8311 ORUS_3322 NC_3140 Hawaii4 Cyclone BxD Idea Cal_544.3 Heidi Florence MDUS_2856 L'Authentique_Orleans Hummi_Ferma Albion FVC_28-1 MSU_9-8 Rapella Cal_143.32 Ourown Madame_Raymond_Poincare Lake_Stevens Robinson MDUS_3413

MDUS_2787 ORUS_1947-2 Liberation_d'Orleans FRA_1580 FVC_16-1 Marmolada(Onebor) ORUS_1239R-21 Pelican MSU_9-3 Ozark_Beauty Marajox Koro103 Winona Nyohou Ooishi-shikinari2 Delmarvel Evangeline Evie-2 SARGENTOCTOPLOID Beaverbelle Ogallala SARGENTDIPLOIDBIN Sans_Rivale Crimson_King Beaversweet

St._Claude

UNK Acknowledgements

This project is supported by the Specialty Crops Research Initiative of USDA‟s National Institute of Food and Agriculture Questions?