Cellulomonas Massiliensis Sp. Nov
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Method for Producing Methacrylic Acid And/Or Ester Thereof
(19) TZZ _T (11) EP 2 894 224 A1 (12) EUROPEAN PATENT APPLICATION published in accordance with Art. 153(4) EPC (43) Date of publication: (51) Int Cl.: 15.07.2015 Bulletin 2015/29 C12P 7/62 (2006.01) C12N 15/09 (2006.01) (21) Application number: 13835104.4 (86) International application number: PCT/JP2013/005359 (22) Date of filing: 10.09.2013 (87) International publication number: WO 2014/038216 (13.03.2014 Gazette 2014/11) (84) Designated Contracting States: (72) Inventors: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB • SATO, Eiji GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO Yokohama-shi PL PT RO RS SE SI SK SM TR Kanagawa 227-8502 (JP) Designated Extension States: • YAMAZAKI, Michiko BA ME Yokohama-shi Kanagawa 227-8502 (JP) (30) Priority: 10.09.2012 JP 2012198840 • NAKAJIMA, Eiji 10.09.2012 JP 2012198841 Yokohama-shi 31.01.2013 JP 2013016947 Kanagawa 227-8502 (JP) 30.07.2013 JP 2013157306 • YU, Fujio 01.08.2013 JP 2013160301 Yokohama-shi 01.08.2013 JP 2013160300 Kanagawa 227-8502 (JP) 20.08.2013 JP 2013170404 • FUJITA, Toshio Yokohama-shi (83) Declaration under Rule 32(1) EPC (expert Kanagawa 227-8502 (JP) solution) • MIZUNASHI, Wataru Yokohama-shi (71) Applicant: Mitsubishi Rayon Co., Ltd. Kanagawa 227-8502 (JP) Tokyo 100-8253 (JP) (74) Representative: Hoffmann Eitle Patent- und Rechtsanwälte PartmbB Arabellastraße 30 81925 München (DE) (54) METHOD FOR PRODUCING METHACRYLIC ACID AND/OR ESTER THEREOF (57) To provide a method for directly and efficiently producing methacrylic acid in a single step from renew- able raw materials and/or biomass arising from the utili- zation of the renewable raw materials. -
Table S1. Bacterial Otus from 16S Rrna
Table S1. Bacterial OTUs from 16S rRNA sequencing analysis including only taxa which were identified to genus level (those OTUs identified as Ambiguous taxa, uncultured bacteria or without genus-level identifications were omitted). OTUs with only a single representative across all samples were also omitted. Taxa are listed from most to least abundant. Pitcher Plant Sample Class Order Family Genus CB1p1 CB1p2 CB1p3 CB1p4 CB5p234 Sp3p2 Sp3p4 Sp3p5 Sp5p23 Sp9p234 sum Gammaproteobacteria Legionellales Coxiellaceae Rickettsiella 1 2 0 1 2 3 60194 497 1038 2 61740 Alphaproteobacteria Rhodospirillales Rhodospirillaceae Azospirillum 686 527 10513 485 11 3 2 7 16494 8201 36929 Sphingobacteriia Sphingobacteriales Sphingobacteriaceae Pedobacter 455 302 873 103 16 19242 279 55 760 1077 23162 Betaproteobacteria Burkholderiales Oxalobacteraceae Duganella 9060 5734 2660 40 1357 280 117 29 129 35 19441 Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas 3336 1991 3475 1309 2819 233 1335 1666 3046 218 19428 Betaproteobacteria Burkholderiales Burkholderiaceae Paraburkholderia 0 1 0 1 16051 98 41 140 23 17 16372 Sphingobacteriia Sphingobacteriales Sphingobacteriaceae Mucilaginibacter 77 39 3123 20 2006 324 982 5764 408 21 12764 Gammaproteobacteria Pseudomonadales Moraxellaceae Alkanindiges 9 10 14 7 9632 6 79 518 1183 65 11523 Betaproteobacteria Neisseriales Neisseriaceae Aquitalea 0 0 0 0 1 1577 5715 1471 2141 177 11082 Flavobacteriia Flavobacteriales Flavobacteriaceae Flavobacterium 324 219 8432 533 24 123 7 15 111 324 10112 Alphaproteobacteria -
Stress-Tolerance and Taxonomy of Culturable Bacterial Communities Isolated from a Central Mojave Desert Soil Sample
geosciences Article Stress-Tolerance and Taxonomy of Culturable Bacterial Communities Isolated from a Central Mojave Desert Soil Sample Andrey A. Belov 1,*, Vladimir S. Cheptsov 1,2 , Elena A. Vorobyova 1,2, Natalia A. Manucharova 1 and Zakhar S. Ezhelev 1 1 Soil Science Faculty, Lomonosov Moscow State University, Moscow 119991, Russia; [email protected] (V.S.C.); [email protected] (E.A.V.); [email protected] (N.A.M.); [email protected] (Z.S.E.) 2 Space Research Institute, Russian Academy of Sciences, Moscow 119991, Russia * Correspondence: [email protected]; Tel.: +7-917-584-44-07 Received: 28 February 2019; Accepted: 8 April 2019; Published: 10 April 2019 Abstract: The arid Mojave Desert is one of the most significant terrestrial analogue objects for astrobiological research due to its genesis, mineralogy, and climate. However, the knowledge of culturable bacterial communities found in this extreme ecotope’s soil is yet insufficient. Therefore, our research has been aimed to fulfil this lack of knowledge and improve the understanding of functioning of edaphic bacterial communities of the Central Mojave Desert soil. We characterized aerobic heterotrophic soil bacterial communities of the central region of the Mojave Desert. A high total number of prokaryotic cells and a high proportion of culturable forms in the soil studied were observed. Prevalence of Actinobacteria, Proteobacteria, and Firmicutes was discovered. The dominance of pigmented strains in culturable communities and high proportion of thermotolerant and pH-tolerant bacteria were detected. Resistance to a number of salts, including the ones found in Martian regolith, as well as antibiotic resistance, were also estimated. -
Phenotypic and Microbial Influences on Dairy Heifer Fertility and Calf Gut Microbial Development
Phenotypic and microbial influences on dairy heifer fertility and calf gut microbial development Connor E. Owens Dissertation submitted to the faculty of the Virginia Polytechnic Institute and State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy In Animal Science, Dairy Rebecca R. Cockrum Kristy M. Daniels Alan Ealy Katharine F. Knowlton September 17, 2020 Blacksburg, VA Keywords: microbiome, fertility, inoculation Phenotypic and microbial influences on dairy heifer fertility and calf gut microbial development Connor E. Owens ABSTRACT (Academic) Pregnancy loss and calf death can cost dairy producers more than $230 million annually. While methods involving nutrition, climate, and health management to mitigate pregnancy loss and calf death have been developed, one potential influence that has not been well examined is the reproductive microbiome. I hypothesized that the microbiome of the reproductive tract would influence heifer fertility and calf gut microbial development. The objectives of this dissertation were: 1) to examine differences in phenotypes related to reproductive physiology in virgin Holstein heifers based on outcome of first insemination, 2) to characterize the uterine microbiome of virgin Holstein heifers before insemination and examine associations between uterine microbial composition and fertility related phenotypes, insemination outcome, and season of breeding, and 3) to characterize the various maternal and calf fecal microbiomes and predicted metagenomes during peri-partum and post-partum periods and examine the influence of the maternal microbiome on calf gut development during the pre-weaning phase. In the first experiment, virgin Holstein heifers (n = 52) were enrolled over 12 periods, on period per month. On -3 d before insemination, heifers were weighed and the uterus was flushed. -
Actinotalea Ferrariae Sp. Nov., Isolated from an Iron Mine, and Emended Description of the Genus Actinotalea
%paper no. ije048512 charlesworth ref: ije048512& New Taxa - Actinobacteria International Journal of Systematic and Evolutionary Microbiology (2013), 63, 000–000 DOI 10.1099/ijs.0.048512-0 Actinotalea ferrariae sp. nov., isolated from an iron mine, and emended description of the genus Actinotalea Yanzhi Li, Fang Chen, Kun Dong and Gejiao Wang Correspondence State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Gejiao Wang Huazhong Agricultural University, Wuhan, Hubei 430070, PR China [email protected] or [email protected] ; A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, designated strain CF5-4T, was isolated from iron mining powder. 16S rRNA gene sequence analysis grouped strain CF5-4T in a single cluster with Actinotalea fermentans DSM 3133T (97.6 % similarity). The major fatty acids T (.5 %) of strain CF5-4 were anteiso-C15 : 0, anteiso-C15 : 1 A, C16 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C17 : 0. The predominant respiratory quinone was MK-10(H4) and the genomic DNA G+C content was 74.7 mol%. The major polar lipids were diphosphatidylglycerol and one unidentified phosphoglycolipid. The peptidoglycan type of strain CF5-4T was A4b, containing L-Orn–D-Ser–D-Asp. The cell-wall sugars were rhamnose, fucose, mannose and galactose. The results of DNA–DNA hybridization in combination with the comparison of phenotypic and phylogenetic characteristics among strain CF5-4T and related micro-organisms revealed that the isolate represents a novel species of the genus Actinotalea, for which the name Actinotalea ferrariae sp. nov. is proposed. The type strain is CF5-4T (5KCTC 29134T5CCTCC AB2012198T). -
Microbiology in Shale: Alternatives for Enhanced Gas Recovery
Graduate Theses, Dissertations, and Problem Reports 2015 Microbiology in Shale: Alternatives for Enhanced Gas Recovery Yael Tarlovsky Tucker Follow this and additional works at: https://researchrepository.wvu.edu/etd Recommended Citation Tucker, Yael Tarlovsky, "Microbiology in Shale: Alternatives for Enhanced Gas Recovery" (2015). Graduate Theses, Dissertations, and Problem Reports. 6834. https://researchrepository.wvu.edu/etd/6834 This Dissertation is protected by copyright and/or related rights. It has been brought to you by the The Research Repository @ WVU with permission from the rights-holder(s). You are free to use this Dissertation in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you must obtain permission from the rights-holder(s) directly, unless additional rights are indicated by a Creative Commons license in the record and/ or on the work itself. This Dissertation has been accepted for inclusion in WVU Graduate Theses, Dissertations, and Problem Reports collection by an authorized administrator of The Research Repository @ WVU. For more information, please contact [email protected]. Microbiology in Shale: Alternatives for Enhanced Gas Recovery Yael Tarlovsky Tucker Dissertation submitted to the Davis College of Agriculture, Natural Resources and Design at West Virginia University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Genetics and Developmental Biology Jianbo Yao, Ph.D., Chair James Kotcon, Ph.D. -
Variation of the Frog Skin Microbiota Across an Environmental Gradient: Taxonomic Diversity and Potential Function
VARIATION OF THE FROG SKIN MICROBIOTA ACROSS AN ENVIRONMENTAL GRADIENT: TAXONOMIC DIVERSITY AND POTENTIAL FUNCTION Brandon J. Varela Department of Biology, Neotropical Environment Option McGill University, Montreal August, 2017 A thesis submitted to McGill University in partial fulfillment of the requirements of the degree of Master’s in Science in Biology Brandon J. Varela, 2017 1 TABLE OF CONTENTS ABSTRACT .............................................................................................................. 4 RÉSUMÉ .................................................................................................................. 5 ACKNOWLEDGEMENTS ...................................................................................... 7 CONTRIBUTIONS OF AUTHORS ......................................................................... 9 Introduction ............................................................................................................ 10 Thesis objectives ......................................................................................................................... 14 Hypothesis 1 ........................................................................................................................................ 15 Hypothesis 2 ........................................................................................................................................ 15 Hypothesis 3 ....................................................................................................................................... -
Data of Read Analyses for All 20 Fecal Samples of the Egyptian Mongoose
Supplementary Table S1 – Data of read analyses for all 20 fecal samples of the Egyptian mongoose Number of Good's No-target Chimeric reads ID at ID Total reads Low-quality amplicons Min length Average length Max length Valid reads coverage of amplicons amplicons the species library (%) level 383 2083 33 0 281 1302 1407.0 1442 1769 1722 99.72 466 2373 50 1 212 1310 1409.2 1478 2110 1882 99.53 467 1856 53 3 187 1308 1404.2 1453 1613 1555 99.19 516 2397 36 0 147 1316 1412.2 1476 2214 2161 99.10 460 2657 297 0 246 1302 1416.4 1485 2114 1169 98.77 463 2023 34 0 189 1339 1411.4 1561 1800 1677 99.44 471 2290 41 0 359 1325 1430.1 1490 1890 1833 97.57 502 2565 31 0 227 1315 1411.4 1481 2307 2240 99.31 509 2664 62 0 325 1316 1414.5 1463 2277 2073 99.56 674 2130 34 0 197 1311 1436.3 1463 1899 1095 99.21 396 2246 38 0 106 1332 1407.0 1462 2102 1953 99.05 399 2317 45 1 47 1323 1420.0 1465 2224 2120 98.65 462 2349 47 0 394 1312 1417.5 1478 1908 1794 99.27 501 2246 22 0 253 1328 1442.9 1491 1971 1949 99.04 519 2062 51 0 297 1323 1414.5 1534 1714 1632 99.71 636 2402 35 0 100 1313 1409.7 1478 2267 2206 99.07 388 2454 78 1 78 1326 1406.6 1464 2297 1929 99.26 504 2312 29 0 284 1335 1409.3 1446 1999 1945 99.60 505 2702 45 0 48 1331 1415.2 1475 2609 2497 99.46 508 2380 30 1 210 1329 1436.5 1478 2139 2133 99.02 1 Supplementary Table S2 – PERMANOVA test results of the microbial community of Egyptian mongoose comparison between female and male and between non-adult and adult. -
1 Supplementary Material a Major Clade of Prokaryotes with Ancient
Supplementary Material A major clade of prokaryotes with ancient adaptations to life on land Fabia U. Battistuzzi and S. Blair Hedges Data assembly and phylogenetic analyses Protein data set: Amino acid sequences of 25 protein-coding genes (“proteins”) were concatenated in an alignment of 18,586 amino acid sites and 283 species. These proteins included: 15 ribosomal proteins (RPL1, 2, 3, 5, 6, 11, 13, 16; RPS2, 3, 4, 5, 7, 9, 11), four genes (RNA polymerase alpha, beta, and gamma subunits, Transcription antitermination factor NusG) from the functional category of Transcription, three proteins (Elongation factor G, Elongation factor Tu, Translation initiation factor IF2) of the Translation, Ribosomal Structure and Biogenesis functional category, one protein (DNA polymerase III, beta subunit) of the DNA Replication, Recombination and repair category, one protein (Preprotein translocase SecY) of the Cell Motility and Secretion category, and one protein (O-sialoglycoprotein endopeptidase) of the Posttranslational Modification, Protein Turnover, Chaperones category, as annotated in the Cluster of Orthologous Groups (COG) (Tatusov et al. 2001). After removal of multiple strains of the same species, GBlocks 0.91b (Castresana 2000) was applied to each protein in the concatenation to delete poorly aligned sites (i.e., sites with gaps in more than 50% of the species and conserved in less than 50% of the species) with the following parameters: minimum number of sequences for a conserved position: 110, minimum number of sequences for a flank position: 110, maximum number of contiguous non-conserved positions: 32000, allowed gap positions: with half. The signal-to-noise ratio was determined by altering the “minimum length of a block” parameter. -
Longitudinal Variations in the Gastrointestinal Microbiome of the White Shrimp, Litopenaeus Vannamei
Longitudinal variations in the gastrointestinal microbiome of the white shrimp, Litopenaeus vannamei Estefanía Garibay-Valdez1,*, Francesco Cicala2,*, Marcel Martinez- Porchas1, Ricardo Gómez-Reyes3, Francisco Vargas-Albores1, Teresa Gollas-Galván1, Luis Rafael Martínez-Córdova4 and Kadiya Calderón4 1 Tecnología de Alimentos de Origen Animal, Centro de Investigación en Alimentación y Desarrollo, Hermosillo, Sonora, México 2 Innovación Biomédica, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, México 3 Universidad Autónoma de Baja California, Ensenada, Baja California, Mexico 4 Departamento de Investigaciones Científicas y Tecnológicas de la Universidad de Sonora, Universidad de Sonora, Hermosillo, Sonora, Mexico * These authors contributed equally to this work. ABSTRACT The shrimp gut is a long digestive structure that includes the Foregut (stomach), Midgut (hepatopancreas) and Hindgut (intestine). Each component has different structural, immunity and digestion roles. Given these three gut digestive tract components’ significance, we examined the bacterial compositions of the Foregut, Hindgut, and Midgut digestive fractions. Those bacterial communities’ structures were evaluated by sequencing the V3 hypervariable region of the 16S rRNA gene, while the functions were predicted by PICRUSt2 bioinformatics workflow. Also, to avoid contamination with environmental bacteria, shrimp were maintained under strictly controlled conditions. The pairwise differential abundance analysis revealed differences -
International Journal of Advanced Research in Biological Sciences
Int. J. Adv. Res. Biol. Sci. (2017). 4(12): 292-299 International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com DOI: 10.22192/ijarbs Coden: IJARQG(USA) Volume 4, Issue 12 - 2017 Research Article DOI: http://dx.doi.org/10.22192/ijarbs.2017.04.12.032 Taxonomic characterization of the chitinolytic actinomycete Cellulomonas chitinilytica strain HwAC11 Gamal M. El-Sherbiny1, Osama M. Darwesh2*, Ahmad S. El-Hawary1 1Botany and Microbiology Department, Faculty of Science (Boys); Al-Azhar University, Nasr City, Cairo, Egypt. 2Agricultural Microbiology Department, National Research Centre, Cairo, Egypt. *Corresponding author: E-mail: [email protected] Mobile: +201155265558, Fax: +20237601036 Abstract Chitinases apply in several useful fields such as agriculture, food industries and environmental applications. Because it helps degradation of fungal cell walls containing chitin and thus accelerates protoplast formation. The alkaliphilic action-bacterial strain HwAC11 was isolated from compost after examine 99 different samples. This isolate exhibited good growth on medium containing chitin as sole carbon source. Macro- and micro–morphological characteristics, enzyme activities, physiological and biochemical properties of the isolate were investigated. It was concluded that the strain HwAC11 is a member of the genus Cellulomonas. The results were compared with the taxonomic characteristics of Cellulomonas members and it was found to be similar to those of Cellulomonas chitinilytica. The phylogenetic analysis based on 16s ribosomal RNA gene sequence confirmed the phenotypic results and the sequences were deposited in gene bank under Cellulomonas chitinilytica strain HwAC11with accession number MH050787. The strain HwAC11 displayed intensive chitinase activity under alkaline conditions. It leads to apply this strain in agriculture field, especially as biological control agent for pathogenic fungi and harmful nematodes. -
List of Bacterial Orders.Pdf
A.Phylum Acidobacteria I. Class Acidobacteria i. Acidobacteriales i Acidobacteriaceae 1 Acidobacterium 2 Edaphobacter 3 Terriglobus II.Class Holophagae i. Acanthopleuribacterales i Acanthopleuribacteraceae 1 Acanthopleuribacter ii. Holophagales i Holophagaceae 1 Geothrix 2 Holophaga B.Phylum Actinobacteria I. Class Actinobacteria i. Sphaerobacteridae i Sphaerobacterales 1 Sphaerobacterineae a Sphaerobacteraceae i Sphaerobacter ii. Rubrobacteridae i Rubrobacterales 1 Rubrobacterineae a Conexibacteraceae i Conexibacter b Patulibacteraceae i Patulibacter c Rubrobacteraceae i Rubrobacter d Solirubrobacteraceae i Solirubrobacter e Thermoleophilaceae i Thermoleophilum iii. Coriobacteridae i Coriobacteriales 1 Coriobacterineae a Coriobacteriaceae i Atopobium ii Collinsella iii Coriobacterium iv Cryptobacterium v Denitrobacterium vi Eggerthella vii Olsenella viii Slackia iv. Actinobacteridae i Bifidobacteriales 1 Bifidobacteriaceae a Aeriscardovia b Alloscardovia c Bifidobacterium d Gardnerella e Metascardovia f Parascardovia g Scardovia 2 Unclassified ii Actinomycetales 1 Streptosporangineae a Thermomonosporaceae i Actinocorallia ii Actinomadura iii Excellospora iv Spirillospora v Thermomonospora b Nocardiopsaceae i Nocardiopsis ii Streptomonospora iii Thermobifida c Streptosporangiaceae i Acrocarpospora ii Herbidospora iii Microbispora iv Microtetraspora v Nonomuraea vi Planobispora vii Planomonospora viii Planotetraspora ix Sphaerisporangium x Streptosporangium xi Thermopolyspora 2 Streptomycineae a Sterptomycetaceae i Actinopycnidium ii Actinosporangium