HPH Schweiz/Suisse/Switzerland, 2006/2007
HPH HIGH PERFORMANCE HORSES SCIENTIFIC NEWS sponsored by TWYDIL®
A DIVERSIFICATION OF THE SOURCES OF NUTRITIVE ELEMENTS COMBINED WITH INGREDIENTS TO STABILIZE INTESTINAL FLORA, HELPS TO STRENGTHEN GROWING HORSES. Dr Brieuc de Moffarts (DVM, Ms, PhD).
HPH 06/07
HPH HIGH PERFORMANCE HORSES SCIENTIFIC NEWS sponsored by TWYDIL®
Editor: Pavesco AG – TWYDIL® Print: Offsetdruck Grauwiller Partner AG Elisabethenstrasse 54 CH-4410 Liestal CH-4010 Basel Copyright: Reprints as well as partial reprints of text Photographers: APRH, Chantilly, France allowed with indication of reference only : Agence Dollar, Le Faulq, France «TWYDIL® HPH 06/07». Sébastien Cox, Liège, Belgium Copy requested. Temps de poses, Ath, Belgium Copyrights of pictures belong to the Trevor Jones, Worlington, UK photographers. Gilly Wheeler, UK Bildagentur Valeria Streun, Oberhasli, Switzerland Robert Bösch, Oberägeri, Switzerland
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Contents Page
A MESSAGE FROM THE PRESIDENT 3
EVALUATION OF AN ORAL SUPPLEMENT ENRICHED WITH GLUCOSAMINE AND CHONDROITINE SULPHATE ON THE JOINT ENZYMATIC BALANCE IN YOUNG HORSES 4
WHERE WILL DUBAI STOP? 11
SPECIAL PRECAUTIONS FOR HOT COUNTRIES 14
UPDATED SCIENTIFIC INFORMATION ON NUTRITIONAL MANAGEMENT OF ENDURANCE ARABIAN HORSES 17
EVALUATION OF A SPECIFIC PROGRAMME FOR ENDURANCE HORSES 20
NEW ANTI-DOPING PRECAUTIONS 26
NEW TWYDIL® DIRECTOR OF PRODUCTION 29
RESPIRATORY TROUBLES 30
THE WORLD’S BEST FOUR-IN-HAND DRIVER 32
INVESTIGATION OF THE SPECIFIC NEEDS OF GROWING HORSES 40
TWYDIL® SUPPORTS RESEARCH 45
TWYDIL® RACING, THE INDISPENSABLE PRODUCT FOR PROFESSIONALS 48
VARENNE AND OTHER FAMOUS ITALIAN HORSES 54
COMPARISON OF HAEMATOLOGICAL PARAMETERS OF 500 HORSES FROM DIFFERENT DISCIPLINES 58
TWYDIL® RANGE OF PRODUCTS 62
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A MESSAGE FROM THE PRESIDENT
1997 1998 1999 2000 2001 2002 2003 2004 2005 2006
In 2006, the year of TWYDIL¨’s 40th birthday, our sales in the world increased by 17 %!
Over the past 10 years, TWYDIL¨ has doubled its turnover by expanding sales within each continent and developing in many emerging markets.
Far from being satisfied, we will redouble our efforts by intensifying our research, diversifying our product range, welcoming new collaborators, and expanding our contacts with veterinary surgeons and the professional horse fraternity.
Together we will continue to work to justify the renowned image of quality and security of TWYDIL¨.
Valère HENRY President
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EVALUATION OF AN ORAL SUPPLEMENT ENRICHED WITH GLUCOSAMINE AND CHONDROITINE SULPHATE ON THE JOINT ENZYMATIC BALANCE IN YOUNG HORSES
Drs Marie Daix, Jean-François Bastin & Nathalie Kirschvink
FUNDP- University of Namur, Veterinary Department, Animal Physiology, rue de Bruxelles 61, B- 5000 Namur
Implication of matrix metalloproteinase on osteoarticular disorder
For the sporting horse, the os- teoarticular pathologies represent the major cause of lameness. Joints are complex structures made up of various entities (see figure 1). The neighbouring bones are covered with joint cartilage at the zones of contact to provide good mobility within the joint. This mobility is en- hanced by the presence of synovial liquid which acts as a lubricant. The whole joint is enclosed within a synovial membrane and stabilised by ligaments and sometimes mus- cles which surround it. The struc- ture of the joint cartilage plays a major role in its movement. It in- cludes both chondrocytes and extra- cellular matrix. The extracellular matrix consists largely of collagen, which supplies the cartilage with its resistance, and proteoglycans and glycoproteins which create the elas- ticity reducing shocks caused by movement. The term " osteoarticu- lar pathologies " in fact includes a large number of different diseases with a common denominator: the destruction of the joint cartilage ex- tracellular matrix (Van Den Boom, et al., 2005). Several authors have shown that this destruction of cartilage follows the activation of pro-inflammatory and enzymatic factors among which
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Figure 1
Neil et al., JAVMA, 2005
so facilitating the destruction of the chondroitine sulphate seem to be extracellular matrix. able to modulate the activity of the These mediators appear to induce MMP and facilitate the synthesis of a chain reaction, in which the various the extracellular matrix (Henrotin, et components join with each other to al., 2002). produce an even more active pro- In vitro studies carried out with a teinase (Nagase, et al., 2006). Re- chondrocyte culture or in vivo with peatedly researchers have shown an orally supplemented rodents showed increase in the activity of the MMP the beneficial effect of these products in case of joint pathology (Brama, on preventing cartilaginous degrada- et al., 2000, Clegg and Cartler, tion (Beren, et al., 2001, Neil, et al., 1999); this increase seems to be the 2005 b). A study showed that an oral first step in the development of carti- supplement composed of glu- lage injury, additionally important cosamine, chondroitine sulphate and and several positive correlations manganese ascorbate delayed the ap- were observed by histological analy- pearance of auto-immune arthritis in sis (Van Den Boom, et al., 2005). laboratory rats (Beren, et al., 2001). Glycosaminoglycans and their In man, there are numerous inves- precursors such as glucosamine or tigations concerning the effects of
the most important seems to be the matrix metalloproteinases (MMP) (Brama, et al., 2004, Neil, et al., 2005 a). The MMP are zinc-dependent en- zymes involved in numerous physio- logical and pathological processes. These proteinases are able to degrade the extracellular matrix. Their activ- ity is subjected to complex control and notably depends on specific in- hibitors: "Tissue Inhibitors of Metal- loproteinases" or TIMP. It is largely the balance between the MMP and the TIMP that defines the proteinase activity. Indeed, the enzyme is inac- tive when bound to its inhibitor. It is only free when its lytic activity ex- presses itself, so creating the capac- ity to split the proteins contained in the extracellular matrix. Some pro- inflammatory factors such as cy- tokines and certain hormones seem capable of activating the MMP and
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Preparation for MMP activity measurement by zymography
climatization the first investigation was undertaken (T0). General phys- ical examinations as well as a spe- cific examination of the locomotive system were made to produce a lameness score for each animal. Joint puncture allowed the extrac- tion of synovial liquid. The ponies glycosaminoglycans and their pre- plement containing, amongst other were divided into two homogeneous cursors on diverse osteoarticular products, glucosamine, chondroitine groups on the basis of size, weight, pathologies. Most of these researches sulphate and harpagophytum on the sex, age and lameness score. During result in a decrease of the seriousness balance of MMP-TIMP in healthy the following six weeks, the ponies and the pain in treated patients. young horses at rest. received an individual supply of These supplements also seem able to supplement A* or B **, mixed into prevent some of the osteoarticular Study presentation their concentrate ration. pathologies, both in man and in ani- Following the six weeks supple- mals (Henrotin, et al., 2002). Sixteen healthy ponies with aver- mentation, a further physical exami- In addition, another study carried age age 2.5 years, average size of out on elderly horses showed that an 1.35 m and 300 kg weight were used oral supplement based on a combina- for this study. The ponies were ac- tion of glucosamine hydrochloride commodated on farms at Centres of and chondroitine sulphate over 12 Ovine research of the University of weeks resulted in a significant in- Namur (Belgium). Their food crease in the length of stride, joint throughout the study consisted of mobility and the duration of move- concentrates given individually ment. This study seems to confirm once a day and hay twice a day. The the beneficial effect of this supple- ponies had access to a meadow for ment on the locomotion of the horse one hour each day. Two weeks were (Forsyth, et al., 2006). allowed for the ponies to become The aim of the present study was acclimatized to their new envi- to estimate the effect of a feed sup- ronment. During this period of ac- Chondrocytes in synovial fluid
Figure 2: Figure 3 Figure 2 MMP2 activity in synovial fluid MMP9 activity in synovial fluid
A* A* B** B** *
* *
T0 T6 anterior T6 posterior T6 knee T6 tarsal T0 T6 MP-Joint MP-Joint joint joint
6 * TWYDIL® ARTRIDIL new formula ** control HPH 06/07
nation was undertaken (T6) identical to the first. Gel d’électrophorèseElectrophoresis permettant gel used de déterminer to determine l’activité MMP The specific examination of the lo- dans le liquide synovial comotive system produced a new MMP activity in synovial fluid lameness score for each pony. Syn- ovial fluid was analysed for the fol- lowing markers: activities of MMP2 and MMP9 and the activity of the TIMP as markers of the enzymatic stress. A cytological analysis of the synovial fluid was also undertaken. The investigators were not aware of Pro-enzymeHuman the identity of supplements A* and MMP9Spots activity d’activité spots de in la synovial MMP9 MMP9Spots activity d’activité spots de in la synovial MMP9 MMP9MMP9 pro- B** until after all the analyses were fluid of a B group pony at T6 fluid of a A group pony at T6 dans la synovie chez un poney dans la synovie chez un poney enzymehumaine completed. du groupe B à T6 du groupe A à T6 usedutilisée as en The study was approved by the lo- standardtant que cal commitee responsible for ethics standard in animal experimentation.
Results e) Cytology: mality and no significant differ- The cytological analysis of the ence was noticed between ponies a) Lameness scores : synovial liquid revealed no abnor- in group A* and group B **. At T0, the ponies’ lameness scores in both groups were very low sug- gesting that none of them pre- sented severe lameness. At T6, the lameness scores were similar to the start and showed no difference between groups A* and B**. There was no significant effect of the supplementation on these low lameness scores.
b) MMP2 activity: At T0 the activity of MMP2 was similar within both groups. This activity did not show any signifi- cant difference after the period of supplementation. Groups A* and B ** thus showed comparable ac- tivities throughout the experiment (see figure 2).
c) MMP9 activity: The activity of the MMP9 in the synovial liquid at T6 was signifi- cantly lower in the horses in group A* compared to the horses in group B **. Additionally, the activities of MMP9 in the joints of the ponies in group A*measured at T6 were dif- ferent from their values at T0. In group B **, this activity at T6 was not significantly different from its value at T0 (see figure 3).
d) TIMP2 activity: At T0 the activity of TIMP2 was similar within both groups. This activity did not show any signifi- cant difference after the period of supplementation.
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In vitro, in a situation of joint en- zymatic stress (greater activity MMP) mimicking a developing os- PERSPECTIVES teoarticular pathology, supplementa- tion with glucosamine and chondroi- flood the market. Does the original tine sulphate induced a reduction in formula tested provide evidence of the activity of the MMP (Byron, et effectiveness? al., 2003, Fenton, et al., 2002). Prof. N. Kirschvink: Our study In our in vivo study, the MMP ac- seems to indicate that a preventive tivity of synovial liquid was low effect exists for the formula tested given that the ponies were healthy making it possible to maintain joint animals. Due to individual variability health. This assumption however within groups A* and B **, no sig- remains to be confirmed when nificant effect of the supplement was young healthy horses are subjected evident. However, a significant mod- Prof. N. Kirschvink to intense physical exercise - a fac- ulation of the MMP9 activity was tor which could not be taken into present in ponies receiving supple- HPH: what does this particular account in the present study. ment A* for 6 weeks suggesting a de- study reveal? crease of the protease activity at the Prof. N. Kirschvink: For the first HPH: Are you surprised by the articular level. time, the evaluation of a chon- absence of effect on the clinical droprotector supplement was car- signs used in this study? Conclusion ried out in horses by clinical exami- Prof. N. Kirschvink: Given that the size of the experimental group In this experiment, supplement A* nation including the investigation was small, that the ponies were clini- had no significant effect on the of joint markers, making it possible cally healthy and that they showed a MMP2 activity or on TIMP2. A sig- to study the effect of the supple- very low lameness score before the nificant decrease of the MMP9 activ- ment directly on the level of the tar- supplementation, a significant im- ity was noticed. These results allow get structures. provement of the clinical signs was us to conclude that supplement A* far from probable. It would be never- modulated the articular enzymatic HPH: For years scientists, for theless interesting to carry out this balance. lack of tangible proof, have been di- vided on the proven effectiveness or type of study on a large scale under not of the chondroprotectors that field conditions.
References Beren J., Hill S.L., Diener-West M. and Rose N.R. Effect of pre-loading oral glucosamine HCl/chondroïtine sulphate/manganese ascorbate combination on ex- ty and new perspectives. 2002. Expert Opinion on in the FUNDP (University of Namur) perimental arthritis in rats. 2001. Experimental biolo- Therapeutic Patents, 1, pp 29-43 then moved to the University of Liège gy and medicine, 226, pp 144-151. Nagase H., Visse R. and Murphy G. Structure and func- Brama P.A.J, Tekoppele J.M., Beekman B., Van El B., where she undertook a doctoral thesis tion of matrix metalloproteinases and TIMPs. 2006. in veterinarian science, studying phy- Barneveld A., and Van Weeren P.R. Influence of deve- Cardiovascular research, 69, pp 526-573. lopment and joint pathology on stromelysin enzyme Neil K.M., Caron J.P and Orth M.W. The role of glucosa- siology under Professor Lekeux (ULg, activity in equine synovial fluid. 2000. Annals of the mine and chondroïtine sulphate in treatment for and Belgium). Her thesis was entitled rheumatic diseases, 59, pp 155-157. prevention of osteoarthritis in animals. 2005 a. Jour- "Study of the role of F2-iso-prostanes Brama P.A.J., Van Den Boom R., Degroot J., Kiers G.H. nal of American Veterinary Medical Association, 7, pp as marker and actor of the lung oxidi- and Van Weeren P.R. Collagenase 1 (MMP1) activity 1079-1088. zing stress”. This specialist in oxi- in equine synovial fluid: influence of age, joint patho- Neil K.M, Orth M.W., Coussens P.M., Chan P.S. and Ca- dative stress then continued with stu- logy, exercise and repeated arthrocentesis. 2004. ron J.P. Effects of glucosamine and chondroïtine sul- Equine Veterinary Journal, 36, pp 34-40. dies in pharmacology and toxicology phate on mediators of osteoarthritis in cultured equi- under Professor Gustin (Ulg, Belgium) Byron C.R., Orth M.W., Venta P.J., Lloyd J.W. and Caron ne chondrocytes stimulated by use of recombinant J.P. Influence of glucosamine on matrix metallopro- equine interleukine-1_. 2005 b. American Journal of where she studied, among other sub- teinase expression and activity in lipopolysacchari- Veterinary Research, 11, pp 1861-1869. jects, enzymatic stress. This horse de-stimulated equine chondrocytes. 2003. American Van Den Boom R., Van Der Harst M.R., Brommer H., enthusiast has been in charge of the Journal of Veterinary Research, 11, pp 1861-1869 Brama P.A.J., Barneveld A., Van Weeren P.R. and De- animal physiology department at the Clegg P.D. and Cartler S.D. Matrix metalloproteinases 2 groot J. Relationship between synovial fluid levels of University of Namur, Belgium, since and 9 are activated in joint diseases.1999. Equine Ve- Glycosaminoglycans, hydroxyproline and general ac- 2005. terinary Journal, 31 (4), pp 324-330. tivity of Matrix Metallo proteinases and the presence Fenton J.I., Chlebek-Brown K.A., Caron J.P. and Orth and severity of articular cartilage change on the pro- M.W. Effect of glucosamine on interleukin-1 conditio- ximal articular surface of P1. 2005. Equine veterinary ned articular cartilage. 2002. Equine Veterinary Jour- journal, 37 (1), pp 19-25. nal Supplement, 34, pp 219-223 Acknowledgements: Forsyth R.K., Brigden C.V. and Northrop A.J. Double blind investigation of the effects of oral supplementa- The authors thank Ing. Marianne tion of combined glucosamine hydrochloride (GHCL) CURRICULUM VITAE Raes, Laetitia Wiggers, Melanie Van- and chondroïtin sulphate (CS) on stride characteri- PROFESSOR KIRSCHVINK dendriessche, Bénédicte Dehand- stics of veteran horses. 2006. Equine Veterinary Jour- nal Supplement, 36, pp 622-625 Nathalie Kirschvink is a native of the schutter and the Laboratory of Pro- Henrotin Y., Sanchez C. and Reginster J.Y. The inhibiti- German-speaking part of Belgium. She fessor Jean-Marie Giffroy for their on of metalloproteinases to treat osteoarthritis: reali- worked for a brilliant university degree contribution to this study.
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&Pbma]kZpZei^kbh]3h_Û\bZeer\^kmbÛ^][rE Nl^][rfhlmh_ma^ln\\^ll_nemkZbg^klbgma^phke]' I:O>L 40 YEARS HAPPY BIRTHDAY TWYDIL® In 2006 TWYDIL¨ celebrated 40 years of existence. TWYDIL¨ was established on 4 April 1966 by Mr Daniel Stofer, who died in 1988. Having already achieved a brilliant career as a banker, Mr Stofer and his associates had the idea to exploit the world wide recognized vitamin experience of the Basel region within the equine sector. With TWYDIL¨ they had the ambition to offer to veterinarians and to horsemen, a scientifically established range of feed supplements pro- duced with the same serious quality as those in the human sector. The success was immediate. Mr Stofer was then assisted by Valère Henry, business man, an agri- cultural specialist with a passion for race horses. After the sudden death of Daniel Stofer, Valère Henry became pres- ident of TWYDIL¨ and the team has been reinforced to develop the international marketing success. Daniel Stofer (left), TWYDIL® initial president with Valère Henry, actual TWYDIL® president. President : Valère Henry Administration : Sandra Schubiger Finance : Barbara Borer Legislation : Michael Putnam Marketing : Philippe Henry Production : Ruedi Bernet Research and Development : Brieuc de Moffarts To make the most of the celebration of this anniversary, last year TWYDIL¨ launched a collection of post stamps, depicting the trade mark. The collection comprising 6 40th anniversary stamps were put on all TWYDIL¨ mail. The TWYDIL¨ management would like to thank their numerous clients throughout the world for their trust in the TWYDIL¨ prod- ucts over the last 40 years. 10 HPH 06/07 WHERE WILL DUBAI STOP? Mel Claremont The Arab world has a long tradi- My first visit to Dubai was back in tels” - try getting a hotel room in one tion with horses being engrained in early 1985 as a young international of the numerous five star hotels today their culture and history. Today, of business executive for what was then and you will find it is not an easy task course, the UAE, and Dubai in par- the largest animal health company in as demand seems to be outstripping ticular, is renowned around the the world, namely Coopers Animal supply even now! world as a centre of excellence for Health. I remember arriving late at The products marketed in UAE for racing. This is spearheaded by the night from London and being bussed the animal health industry at that ruling family’s love of horses, from the plane to the relatively small time were predominantly for sheep, which of course goes far beyond a terminal building and then taking a goats, cattle, camels and poultry, in- hobby. very quick taxi ride through a traffic- cluding falcons. Horses were more in Dubai is one of the leading equine free Dubai to the Sheraton Hotel by the minority category along with empires in the world and arguably the Creek; this was one of only a other companion animals such as the premier one, with internationally handful of five star hotels but at the dogs and cats. recognized names such as Godolphin time I, like many other people, were The changes in Dubai since my Ð probably the most successful rac- highly impressed by the futuristic de- first visit started off slowly, the odd ing organization in the history of the sign of the hotel both externally and new office block, apartments and ho- sport for kings. Combined, the Mak- internally. tels and then the pace seemed to in- toum family’s studs; Shadwell, Dar- The World Trade Centre building crease dramatically once the con- ley and Gainsborough, are one of the towered over everything else and struction started in Sheikh Zayed most prestigious breeding empires could be seen for many miles around Road. Now, as many of you will the industry has ever seen. Dubai from all directions. Driving know as it is widely broadcasted, the Racing under rules in the United from Abu Dhabi one would spot the construction going on in Dubai is un- Arab Emirates began in November tower and know that the centre of paralleled Ð perhaps with the excep- 1991 at Abu Dhabi Racecourse, with Dubai was not too far off; very few tion of China. Everything new in the first race at Nad Al Sheba taking buildings and no significant tower Dubai seems to be exceptional one place in February 1992. From that blocks were in existence on Sheikh way or another, either the biggest, the historic date Dubai racing never Zayed Road. As the number of five first or, as is often the case, simply looked back and quickly became star hotels grew, people would say the best! Some of the architecture recognised by the world’s racing au- "who is going to stay in all these ho- must be seen to be believed. thorities, but nobody could have fore- 11 HPH 06/07 The Carnival also features two ‘double header’ weekends, the first of which ran from February 8-9. Thurs- day’s action centred on the Al Mak- toum Challenge Round 2, over 1800 metres, and the Listed Cape Verdi, a 1600 metres contest for fillies and mares which was worth $150,000. Friday, February 9 saw the first Classic of the season, the Group 3 UAE 2,000 Guineas, which is worth $250,000 and limited to three-year- old colts. The fillies had their turn the following week in the Listed UAE 1,000 Guineas, which was won in 2006 by UK raider Vague, trained by Jeremy Noseda. March saw the action really heat up, as March 1 was ‘Super Thursday,’ an evening packed with trials for the Dubai World Cup meeting four weeks after. The Group 2 Maktoum Challenge Round III, run over the full Dubai World Cup course and dis- seen how quickly it would grow. Sheba, the 2007 Carnival ran for 11 tance, is an excellent trial for the The world first knew of Dubai rac- weeks, from January 18 until March world’s richest race and was won in ing in 1993 when Nad Al Sheba 8. Worth a staggering $10 million in 2006 by Electrocutionist, who went hosted the Dubai International Jock- total, it saw the best horses and jock- on to World Cup glory for Godol- eys’ Challenge which was won by the eys from around the world battle it phin. United States of America. Then in out on the dirt and turf tracks. The Purebred Arabians also have 1996 racing literally took off with the The action always kicks off with their opportunity in the Al Maktoum inaugural running of the the Group 3 Al Maktoum Challenge Challenge III (PA), before the action US$4,000,000 Dubai World Cup, Round 1, worth $200,000. Run over swings back to the Thoroughbreds in won by the awe inspiring champion 1600 metres on dirt, it is traditionally the Group 3 City of Gold, run over Cigar. the first preparatory race for likely 2400 metres on the turf track, the In just two years the Dubai World Dubai World Cup contenders and trial for the Dubai Sheema Classic. Cup was granted Group One status was won in fine style in 2006 by Blink and you miss it; the sprinters and in that year it was won by the Princess Haya’s Blatant. fly down the straight six furlong dirt Kentucky Derby winner Silver Charm. The following year history was made yet again when winners of the Kentucky Derby, the Epsom Derby and the Italian Derby lined up for the first time in the same race. Beyond all shadow of a doubt, the Dubai World Cup had become a truly global event. It did not take long before Dubai World Cup day became the sporting and social highlight of the Gulf re- gion, and in 1999, at 5 million US dollars, it became the world’s richest race ever run. In 2000 the prize was raised to US$ 6 million, and with to- tal prize money on the day of more that US$ 15 million, it stood as the richest day of racing on the planet. In 2004, racing in Dubai took an- other huge leap forward with the cre- ation of the Dubai International Rac- ing Carnival, and this year saw the fourth such event. Held at Nad al 12 HPH 06/07 high goal tournaments all over the world. In the UAE, the season runs during the cooler winter months from No- vember through until April. It is in the last few months, however, that the action really starts to sizzle, with a number of high-goal tournaments, in- cluding the Cartier Cup, the Kinnarps Cup, the President’s Cup and the an- nual spectacle of beach polo. Clearly the vast majority of horses in UAE are in one form of competi- tion or another. However, the leisure market, which was virtually non-ex- istent a few years back, is now grow- ing at a staggering pace. New facili- ties, such as the Dubai Polo Club at Arabian Ranches, as well as the forthcoming Plantation and Al Ka- heel horse parks in the simply stag- gering Dubailand are being added to established venues such as Emirates track in the Group 3 Mahab al Shi- from January 10-12 and incorporated Riding Centre & Desert Palm Polo maal, while Godolphin Mile candi- a qualifying round of the Arab Club. Interest in leisure riding dates trial in the Burj Nahaar. Dubai League. With the UAE team picking reaches across the cosmopolitan Duty Free hopefuls go through their up an excellent bronze medal at the community in UAE with the various paces on the Turf in the Group 2 Asian Games in Qatar, showjumping clubs popular with both the local Jebel Hatta, run over 1777 metres. is a sport which will continue to grow population and expatriates alike. Thursday, March 8 saw the curtain in the country. Without any doubt UAE is a horse come down on the Dubai Racing The Qatar games also saw the lover’s paradise covering everything Carnival, which started in fine style UAE, spearheaded by Sheikh Rashid including flat racing, endurance, with the $250,000 UAE Oaks, won in bin Mohammed Al Maktoum, who show jumping, dressage and polo or 2006 by Herman Brown’s Imperial also claimed individual gold, take just a hack in the desert or a trot Ice. gold in Endurance. Excelling in this along the beach at sunset. Then it was all eyes on March 31 discipline for many years, the UAE and the 12th running of the Dubai has world-class Endurance facilities Thanks to RaceWeek & Emirates World Cup meeting. The 2007 entries in both Dubai and Abu Dhabi, and Racing Authority for their contribu- on the seven race card included is at the forefront of the push for it tion. Breeders’ Cup Classic winner Inva- to be included in the Olympic sor, Epsom Derby winner Sir Percy, Games. as well as Breeders’ Cup Turf winner Polo is the latest horse sport to re- Red Rocks and Godolphin’s new su- ally take off in Dubai. The sport in perstar Discreet Cat, it was a night to the Emirates does, however, date remember. back over 30 years when the Dubai On the showjumping scene, the Polo Club was created at Abu annual Dubai Showjumping Champi- Khadra. Now there are three well-es- onships, held at the Emirates Riding tablished clubs in the UAE and more Centre, have become the focal point on the way, while the Dubai polo of the season. This year’s event ran team is competing with the best in TWYDIL¨ IS DISTRIBUTED IN THE UAE EXCLUSIVELY THROUGH 13 HPH 06/07 SPECIAL PRECAUTIONS FOR HOT AND HUMID COUNTRIES Ruedi Bernet, Director of Production QUESTION : standard pail was still above the dec- VITAMIN C Do vitamins resist heat and hu- laration after more than 7 months in Only vitamin C in the standard midity? the oven. There was no significant pail of TWYDIL¨ RACING was, af- The question is important for users difference with the vacuum-packed ter 226 days in the oven, 21% below in hot and humid climates who are product. the declaration whereas the vacuum- not able to store TWYDIL¨ products under optimal conditions. STUDY : Stability of vitamin A TWYDIL¨ asked DSM to under- at 35¡ C and 80% relative humidity take a study by placing a pail of TWYDIL¨ RACING in an oven at 35¡ and 80% relative humidity for 7 months. The concentrations of the more la- bile vitamins were then analysed af- Declared value ter 38 days, 102 days and 226 days. For comparison, another pail with TWYDIL¨ RACING vacuum-packed in an aluminium bag was placed un- der the same conditions. RESULTS : 38 days 102 days 226 days VITAMIN A Although such storage conditions in normal pail with additional precautions are absolutely not recommended, the concentration of vitamin A in the 14 HPH 06/07 packed product still conformed to the declaration. Stability of vitamin C at 35¡ C and 80% relative humidity CONCLUSIONS : As the standard pails allow Declared value good conservation of the product under normal storage conditions, they cannot prevent a small degradation of vitamin C after 7 months when exposed to heat and humidity. Consequently, for clients in hot and humid countries, TWYDIL¨ RACING and TWYDIL¨ STUD will now be packed in polyester-alu- 38 days 102 days 226 days minium bags before being put in the in normal pail with additional precautions pails. The additional polyester-aluminium precau- Oven in which TWYDIL® RACING was placed for more than 7 months. tion for hot countries. 15 ÇäÊ "1 /, -ÊÊ ,"--Ê/ Ê7", / iÊ/79 ÁÊÀ>}iÊvÊ«À`ÕVÌÃÊ ÃÊ>Û>>LiÊÊÇäÊVÕÌÀiÃ°Ê -iÛiÀ>ÊVÕÌÀiÃÊ >ÛiÊÀiViÌÞÊ LiiÊ>``i`ÊÌÊÌ iÊÃÌÊÜ iÀiÊ Ì iÊ-ÜÃÃÊ«À`ÕVÌÃÊ>ÀiÊV>ÞÊ `ÃÌÀLÕÌi`° *3"/ 5)&/&5)&3-"/%4 4065),03&" vÊÞÕÊÜà ÊÌÊLiViÊ>Ê/79 ÁÊ`ÃÌÀLÕÌÀÊÊ>ÞÊvÊÌ iÊ VÕÌÀiÃÊÜ iÀiÊÌ iÊ«À`ÕVÌÃÊ>ÀiÊÌÊÞiÌÊ>Û>>Li]ÊVÌ>VÌÊ vJÌÜÞ`°VÊÀʳ{£ÊÈ£ÊÓÇÓÓÎÇÓ° ^&fZbe3bg_h9mpr]be'\hf HPH 06/07 UPDATED SCIENTIFIC INFORMATION ON NUTRITIONAL MANAGEMENT OF ENDURANCE ARABIAN HORSES Dr Brieuc de Moffarts (DVM, Ms, PhD) Director R&D, PAVESCO-TWYDIL¨ Numerous studies on endurance Forage acts as a: In order to diversify the ration, horses have been published in the last Ð Gut transit regulator, other sources of energy are also im- decade. These studies evaluated the Ð Stomach lining protector, portant. Popping or flaking cereals different limitation points in this dis- ÐDrinking stimulator, (oats, barley and corn) can be advan- cipline such as: Ð Reserve of water and electrolytes tageous for maintaining the body for prolonged exercise, weight during the training and allow- a) Metabolic problems linked to en- Ð Slow release energy provider (so ing glycogen formation during train- ergy use, fatigue and muscle called: volatile fatty acids). ing recovery. tonicity. Oil supplementation can also be an b) Dehydration and electrolytic mis- For all these reasons, the quantity advantage for endurance horses. Ef- balance; and the quality of hay is one of the fectively, progressive inclusion of oil c) Locomotor problems. most important aspects of the nutri- in the ration, around 5% of the total tional management of Arabian en- ration during training and 8-10% It has been shown that these prob- durance horses. during the weeks before the race lems can be managed by an appropri- helps to reduce the use of glycogen (a ate nutritional approach. Practically the trainer must pro- type of glucose store (energy)) and Forage remains the basis of nutri- vide adequate amounts of hay until improve the use of fatty acids (an- tion of endurance horses. The ratio the morning of the race. During each other energy source). between forage and concentrate stage of the race, hay or other type of Caution ! When a trainer gives oil, should be at least 70/30. The use of fibres, such as moist sugar beet pulp, he has to increase the level of magne- alfalfa can be worthwhile providing should be given. Finally, hay alone sium, vitamin E and L-carnitine in it does not exceed more than 20% of should be given during recovery after the ration in order to prevent oxida- the total forage. the race. tion processes and improve the ener- 17 HPH 06/07 Table I: One of the most studied and con- Indicative values for specific needs during training of Arabian endurance horses troversial aspect in endurance horses Fat soluble vitamins Watersoluble vitamins is the management of water and elec- trolytes. Vitamin A IU 30000 Thiamine mg 20 During the training period, the Vitamin D3 IU 2000 Riboflavin mg 7.5 trainer in association with his veteri- Vitamin E IU 500 Pyridoxine mg 7 narian should determine the adequate Vitamin K mg 2.5 B12 mg 0.07 level of electrolytes depending on Niacin mg 30 Trace elements Pantothenic acid mg 12 temperature, humidity and work Biotin (Vitamin H) mg 0.5 load. Sweat loss can be scientifically Copper mg 40 Folic acid mg 18 measured and then compensated by Iron mg 375 Choline mg 280 an adequate level of electrolytes. Ap- Manganese mg 150 Ascorbic acid mg 750 proximately, the amount of sweat Zinc mg 150 produced, is around 0.5 Ð 1, 1-2, 2-5 Cobalt mg 1.7 Iodine mg 5 and 7-8 L/hour of work for light, Selenium mg 2.25 moderate, hard and very heavy exer- cise respectively Table II lists the most important elements contained in equine sweat and the appropriate getic fat metabolism specifically §- GADIL and TWYDIL¨ PROTECT amounts of electrolytes to give to oxidation (utilisation of the fatty PLUS daily, for 28 days before an in- horses to replace the loss. This calcu- acids as energy source). ternational race. lation must be done for each day of The TWYDIL¨ products were the training for a week and then aver- Practically, a blend of different given in order to provide omega-3 age supplementation per day can be oils improves the energy utilisation fatty acids, dimethylglycine, L-carni- determined. for long-distance exercise. tine, branched amino acids, chromium, orotic acid and additional Practically, give a good propor- In addition, the ration has to be supplementation of antioxidant vita- tion of hay in the ration, electrolytes balanced with an adequate level of mins such as, 5,000 IU vitamin E and on a daily basis, not by week, and al- vitamins and trace elements (Table I) 10 g of active vitamin C. ways with fresh water ad libitum. in order to maintain the entire body The results indicate that, more metabolism during the training. than maintaining all the physiologi- During a race, endurance horses cal values (vitamins, trace elements lose 3-7% of their body weight as A trial has recently been con- and electrolytes levels) during the sweat. ducted on the effect of an original last part of the training, the The highest priority is water re- endurance TWYDIL¨ programme TWYDIL¨ programme significantly placement during the race. To be sure by a team led by Professor Lekeux improved the antioxidant blood ca- the water is appetizing, some trainers (ULg). pacity (figure 1) and the platelet ag- bring their own water. Electrolyte In this trial, supplemented and gregation factor (figure 2). supplementation during the race can- control (placebo) groups both re- not replace the total electrolyte loss ceived the normal recommended Practically, the TWYDIL¨ pro- but will stimulate drinking. It’s es- amounts of vitamins, macro-ele- gramme improves antioxidant de- sential to keep giving electrolyte for ments (electrolytes) and trace-ele- fences of endurance horses and also several days after the race, to allow ments. In addition, the supplemented allows a good microcirculation dur- the replenishment of the stock in the group received TWYDIL¨ OME- ing the race. gut trough the forage. 18 HPH 06/07 Table II: sense at each stage of the selection, Indicative values for the sodium, potassium and chloride contents of equine sweat. breeding, growing, training and rac- Electrolyte Sweat concentration Amount to be ingested (g) to ing. (g/L) replace one litre of sweat Management of nutrition during growing, especially in terms of en- Sodium (Na) 3.1 3.45 ergy, of calcium-phosphor ratio and Potassium (K) 1.6 2 copper-zinc ratio, is critical. Chlorine (Cl) 5.3 5.5 During training, use of a chon- (P. Harris, ENUCO 2005) droprotector can be beneficial as a long term treatment. Caution : don’t use a too concen- this way, 50% of the total water in- Conclusion: In such a competitive trated electrolyte paste as it is proved take was given with TWYDIL¨ discipline with incremental pressure to increase the risk of gastric ulcers. ELECTROLYTES. on horses, a professional nutritional Also, at each stage of the race, give Lameness during endurance races approach is indispensable to help free access to fresh water, with or is still very frequent and is a major Arabian endurance horses reach and without electrolytes or access to a cause of disqualification. Lameness maintain their best physiological po- mash with a high level of moist sugar also induces artificial increase of tential for a maximum length of time. beet pulp. heart rate frequency and indirect During a trial conducted by Pro- muscle stiffness. fessor Kirschvink (FUND), in which In order to prevent these particular SOURCES: a standardised dehydration was cre- problems, the interrelation between Schott et al., 2006, ICEEP ¨ Rose et al., 1986, Br Vet J ated, TWYDIL ELECTROLYTES veterinarians, trainers, farriers and Harris , 2005, ENUCO was added to a known amount of wa- breeders is very important. The ex- Goachet et al., 2006, AVEF ter and given to horses in solution. In pression "no foot no horse” makes Forsyth et al., 2006, ICEEP 19 HPH 06/07 EVALUATION OF A SPECIFIC PROGRAMME FOR ENDURANCE HORSES Drs Audrey Fraipont, Tatiana Art & Pierre Lekeux Liège University, Faculty of Veterinary Medicine, Equine Sport Medicine Centre 20, Bld de Colonster, B-4000 Liège The aim of this study was to assess 2) that the supplement could reduce runs of 20 to 40 km. Between these the effects of dietary n-3 fatty acids, the oxidative stress potentially in- runs a break of pre-determined dura- electrolytic and antioxidant supple- duced by intensive training. tion is imposed. During these breaks, mentation in endurance horses per- as well as at the beginning and the forming at a high level. Amongst all the equestrian disci- end of the race, the horses have to be Thanks to this study, we were able plines, the endurance ride is probably seen by veterinarians who assess to demonstrate in these horses: the most demanding. The horses have their clinical state and recovery, al- 1) that the administration of the to cover distances from 20 to 160 km lowing them to continue or not. above-mentioned products in- in one day at a sustained and steady Performing a long lasting, low in- creased the blood level of the dif- pace, and on an irregular terrain in tensity exercise, the horse’s system ferent component, demonstrating sometimes extreme weather condi- adopts a different strategy from the that way the good biodisponibil- tions. one seen during short intense exer- ity of the endurance programme*; These races are subdivided into cise (such as sprint races). 20 ** TWYDIL® RACING (37,5g/day) + TWYDIL® ELECTROLYTES (40g/day) + TWYDIL® PROTECT PLUS (60g/day) + TWYDIL® OMEGADIL (60ml/day) HPH 06/07 domination. The body can fully con- trol the exercise. Knowing that a horse loses 2-8L of sweat every hour depending on workload, temperature and humidity, increasing to more than 15L in ex- ceptional conditions (Carlson, et al. 1983), we must appreciate the im- portance of good rehydration during exercise to maintain blood volume and thus the correct cardiac output. The quantity of electrolytes (present in large concentration in the sweat) is also very important in this type of exercise because electrolytes are es- sential for the systemic (intra and ex- tra cellular fluid balance) and effi- cient cellular (nervous conduction, muscle depolarisation) functions. The rapid oxygen supply from the lungs to the muscles depends on good blood volume and cardiac out- put, and the integrity of blood ves- sels and red blood cells. Red blood cells have to distort easily in order to enter the smallest blood vessels. Re- cent studies indicate that the admin- istration of (n-3) fatty acids can modulate the distortion ability of the red blood cells. Thanks to (n-3) fatty acids, red blood cell membranes develop better elasticity (Portier, et al. 2006). Exer- cise seems to decrease the fluidity of the membranes and potentially the cellular distortion (O’Connor, et al. 2004). These (n-3) fatty acids could reduce platelet aggregation (respon- sible for thrombus formation poten- tially induced by exercise or inflam- The energy metabolism is almost entirely aerobic: after a short period of anaerobic metabolism observed at the very beginning of the muscular work, the muscles function only with cellular respiration (Art, et al. 2000). This cellular respiration is en- tirely dependent on a persistent and regular supply of oxygen and ener- getic substrate. The endurance horse has to establish a stable blood circu- lation and distribution to all the dif- ferent parts of the body. The circula- tion cannot be directed only to the muscles at the expense of other or- gans because it might expose the horse to different pathologies such as colic, laminitis or hyperthermia. With a good general circulation, thermo-regulation, digestion and lo- comotion can occur without any 21 HPH 06/07 mation) (Zamaria, 2004, Din, et al. the same protocol was applied. Blood 2004). function analyses were performed. The red blood cells’ distortion The following factors were investi- ability can also be altered by oxida- gated: cellular lesion markers, antioxi- tive stress (Baskurt, 1999). This ox- dant blood capacity, haematology and idative stress is described as the syn- fatty acids of the red blood cells’ drome in which the pro-oxidant radi- membranes. Samples were collected cals overcome the anti oxidant capac- from each horse in each group, on the ities of the body (see HPH 2002- morning prior to the first supply of 2003, p. 4-10). This imbalance re- supplement (T0) and after four weeks sults from the formation of reactive of supplementation (T1). oxygen species (ROS) within the me- tabolism (this process is exacerbated by the increase of oxygen consump- RESULTS tion during exercise, for example). ROS have deleterious effects on TWYDIL¨ SUPPLEMENT lipids, proteins and DNA. It had been EFFECTS: shown earlier that exercise induces oxidative stress and the administra- a) Electrolytes plasmatic tion of antioxidants can help de- concentration: crease the deleterious effects of this The additional TWYDIL¨ fatty acid oxidative stress in sport horses (de and antioxidant supplement enabled Moffarts, et al. 2005, Deaton, et al. supplemented horses to keep their 2002, Kirschvink, et al. 2002b). This study’s aim is to assess the ef- fects of an antioxidant supplement enriched with electrolytes and se- EffeEffectEt ffeduct pr ofog enduranceerammendurance enduran programmeprogramce suron on water la water capacité soluble soluble antioxydante antioxidant antioxidant lected (n-3) fatty acids on oxidative hydrophilecapacitycapacity of duplasma of pl plasmaasma stress and its consequences in high CAHP(nmolACW (nmol équ. éq aa/ml)u. aa/ml) level endurance horses during a pe- 160 riod of intensive training. 120 STUDY PROGRAMME PlaceboPlac group**ebo 80 Nine national and international SupplementedTraité* group* healthy endurance horses (CEI**, 40 CEI*** and CEI****, 120 km and 160 km races) were selected in four 0 endurance stables located in Bel- d0j0 j28d28 gium, Luxembourg and France. Gen- TeTime mps eral examination was performed in CEMESPO (Faculty of Veterinary Medicine in Liège) by a veterinarian (respiratory system examination, car- diac examination…). Throughout the EffectEffect of of endurance endurance programme program onon fat fast soluble soluble antioxidant antioxidant entire study the horses’ diet was stan- capacitycapacity of ofplasma plasma dardized (good quality hay and main- tenance grain diet) in order to avoid ACL(nmol équ. Trol./ml) any variances due to diet. Horses were divided into two groups: the first group received a com- mercially available balanced supple- ment (placebo group**), the second PlaceboPlacebo** group** group received, in addition to the SupplementedSupplémenté* group* placebo, an oral antioxidant supple- ment enriched in selected (n-3) fatty acids (endurance programme group*). Horses received the supplement dur- ing four weeks prior to an important d0 d28 race. After four weeks wash out period Time the supplements were switched and ** TWYDIL® RACING (37,5g/day) + TWYDIL® ELECTROLYTES (40g/day) + TWYDIL® PROTECT PLUS (60g/day) + TWYDIL® OMEGADIL (60ml/day) 22 ** TWYDIL® RACING (37,5g/day) + TWYDIL® ELECTROLYTES (40g/day) + placebo HPH 06/07 plasma electrolytes concentration c) Red blood membranes fatty is done with high quality products, stable during the intensive training acids profile: associated with a high concentration period (sodium, potassium, chlo- In the supplemented group, a rise in of antioxidants. It was shown that op- ride, calcium). The maintenance of omega-3 type membrane fatty acid is posite effects might be observed with the hydrolytic and electrolytic bal- clearly noted, especially docosa- low quality fatty acids (Song, JH. et ances is a priority for the endurance hexaenoic acid (DHA) and eicos- al. 2000, Palozza, et al. 1996). horse. apentaenoic acid (EPA). The gain with this last fatty acid was statisti- GENERAL CONCLUSIONS b) Antioxidant protection: cally significant. DHA and EPA have The lipophilic antioxidant ability considerable effects on the cardio- The tested endurance programme* of the plasma (plasma ACL, corre- vascular system and on the inflam- might prevent an oxidant/antioxidant lated to the vitamin E and to the bêta- matory phenomenon in horse species imbalance potentially induced by carotene) was significantly increased (decreased heart rate, effects on ratio training. It increases the proportion of after the endurance programme* sup- LT4/LT5, on macrophages’ func- omega-3 fatty acids in the red cell plementation period, as well as the tions,…) (0’Connor, et al. 2004). membranes which could improve their hydrophilic antioxidant ability of the The ratio of arachidonic acid fluidity and thus their ability to circu- plasma (plasma ACW, correlated to (omega-6) to eicosapentaenoic acid is late in the capillaries. It also helps to the vitamin C) that increased even significantly reduced in the supple- maintain correct plasma electrolytes though ACW decreased slightly for mented group, this ratio being corre- concentration necessary for every sin- the placebo group**. The endurance lated with the platelet aggregation gle endurance exercise. programme* seems to partially coun- ability. The additional omega-3 type terbalance the imbalances that can fatty acids in cell membranes is corre- PERSPECTIVES occur throughout a race season in lated with the red blood cells mem- horses that are submitted to intensive brane fluidity and to a lower blood Knowing that intense exercise can training (de Moffarts, et al. 2004). viscosity, and helps to maintain lower induce thrombi in small vessels, it This observation confirms the heart rate (O’Connor et al. 2004). would be very interesting to study good absorption and excellent It is important to underline that the more specifically the effects of (n-3) bioavailability of the fatty acids and addition of fatty acids to endurance fatty acids on platelet aggregation antioxidants contained in the en- horses’ diets (as is frequently the and its consequences on general durance programme*. case) has beneficial effects only if it blood circulation. ** TWYDIL® RACING (37,5g/day) + TWYDIL® ELECTROLYTES (40g/day) + TWYDIL® PROTECT PLUS (60g/day) + TWYDIL® OMEGADIL (60ml/day) ** TWYDIL® RACING (37,5g/day) + TWYDIL® ELECTROLYTES (40g/day) + placebo 23 HPH 06/07 REFERENCES ART T., AMORY H., LEKEUX P. Notions de base de physio- Effect of endurance program on platelets aggregation index Effect of endurance programme on platelet aggregation index logie de l’effort. Pratique Vétérinaire Equine 2000, 32, n° afterafter 4 weeks supplementationsupplementation spécial, 7-14. BASKURT O.K., MEISELMAN H.J. Susceptibility of equine PlatPlateletelets aggregation aggregation index index erythrocytes to oxidant-induced rheologic alterations. Am J Vet Res. 1999, 60, 1301-6. CARLSON G.P. Thermoregulation and fluid balance in the exercising horses. In SNOW D.H., PERSSON S.G.B., ROSE R.J. (eds): Equine Exercise Physiology. Cam- bridge, England, Granta editions, 1983, 291. PlaceboPlacebo** group** DEATON C.M., MARLIN D.J., ROBERTS C.A., SMITH N., HARRIS P.A., KELLY F.J., SCHROTER R.C. Antioxidant SupplementedSupplémenté* group* supplementation and pulmonary function at rest and exercise. Equine Vet. J., 2002, Suppl 34, 58-65. DE MOFFARTS B., KIRSCHVINK N., ART T., PINCEMAIL J., MICHAUX C., CAYEUX K., DEFRAIGNE J-O., LEKEUX P. Impact of training and exercise intensity on blood an- tioxidant markers in healthy Standardbred horses. d0 d28 Equine and Comparative Exercise Physiology 1 (2004) 211-220. Time DE MOFFARTS B., KIRSCHVINK N., ART T., PINCEMAIL J., LEKEUX P. Effect of oral antioxidant supplementation on blood antioxidant status in trained thoroughbred horses. The Veterinary Journal 169 (2005) 65-74. DIN J.N., NEWBY D.E., FLAPAN A.D. Omega 3 fatty acids and cardiovascular disease-fishing for a natural treat- EffectEffeEffe ofct t endurance ofdu e prnduranceogramme programme program endurance onon membranemembrane sur le profil eïcosapentaenoic d'acide ment. BMJ (2004), 328, 30-35. eïcosapéïencotaesanoicpentaènoïquefatty fatty acid acid composition composition membranaire of erythrocytesof erythrocytesdu globule rouge KIRSCHVINK N., FIEVEZ L., BOUGNET V., ART T., DE- GAND G., SMITH N., MARLIN D., ROBERTS C., HARRIS P., LEKEUX P. Effect of nutritional antioxidant supple- EPAEPA mentation on systemic and pulmonary antioxidant sta- 0,8 tus, airway inflammation and lung function in heaves-af- fected horses. Equine Vet. J., 2002b, 34, 705-712. 0’CONNOR C.I., LAWRENCE L.M., LAWRENCE A.C., JA- 0,6 NICKI K.M., WARREN L.K., HAYES S., The effect of die- tary fish oil supplementation on exercising horses. J. Anim. Sci. 2004, 82, 2978-2984. Placebo**Placebo 0,4 Placebo group** PALOZZA P., SGARLATA E., LUBERTO C., PICCIONI E., SupplementedSupplémenté*Traité* group* ANTI M., MARRA G., ARMELAO F., FRANCESCHELLI P., BARTOLI G.M. N _ 3 fatty acids induce oxidative modifi- 0,2 cations in human erythrocytes depending on dose and duration of dietary supplementation. American Journal of Clinical Nutrition, 1996, 64, 297–304. 0 PORTIER K., DE MOFFARTS B., FELLMAN N., KIRSCH- VINK N., MOTTA C., LETELLIER C., RUELLAND A., VAN j0d0 d28 j28 ERCK E., LEKEUX P. and COUDERT J. The effects of dietary N-3 and antioxidant supplementation on erythro- Time Te mps cyte membrane fatty acid composition and fluidity in exercising horses. Equine Exercise Physiology 7, 2006, 36. SONG J.H., FUJIMOTO K., MIYAZAWA T. Polyunsaturated (n _ 3) fatty acids susceptible to peroxidation are increa- sed in plasma and tissue lipids of rats fed docosahexae- Effet duEffe prctog oframme endurance endu programrance sur on lemembrane profil en acide gras noic acid-containing oils. Journal of Nutrition, 2000,130, Effect of endurance programme on membrane docosahexaenoic 3028–3033. dodoccosahexaenoicosahexaèfattynoïque acid fatty composition membranaire acid composit of erythrocytes duion gl ofobule erythrocytes rouge ZAMARIA N. Alteration of polyunsaturated fatty acid status and metabolism in health and disease. Reprod. Nutr. DHADHA Dev., 2004, 44, 273-282. 0,6 0,4 PlaceboPlacebo**Placebo group** SupplementedSupplémenté*Traité* group* 0,2 0 d0j0 j28d28 TimeTe mps ** TWYDIL® RACING (37,5g/day) + TWYDIL® ELECTROLYTES (40g/day) + TWYDIL® PROTECT PLUS (60g/day) + TWYDIL® OMEGADIL (60ml/day) 24 ** TWYDIL® RACING (37,5g/day) + TWYDIL® ELECTROLYTES (40g/day) + placebo HPH 06/07 NEW ANTI-DOPING PRECAUTIONS FOR US, YOUR SECURITY HAS NO PRICE Philippe Henry, Marketing Director TWYDIL¨ TWYDIL¨’s objective has always anti-doping controls which each pro- dertakes such external anti-doping been to develop a range of products duction batch of the whole range has controls (control of product, but also which are both efficient and safe. to undergo. on urine and blood of a horse having From the beginning TWYDIL¨ As far as we know, up to now no received an overage). has been transparent in communicat- other manufacturer of feed supple- The various certificates estab- ing to its clients the list of external ments for horses, systematically un- lished by LCH (Central Laboratory of the International Equestrian Fed- eration and official French laboratory of anti-doping control for animals) are available on our website: www.twydil.com. In summary almost all TWYDIL¨ products* as certified by LCH can be used without risk up to the day of the competition. This general principle has certain provisos: 1) Depending on the discipline and the country, it is the user’s re- * Exceptions: Recently a waiting time of 48 hours has been recommended for TWYDIL¨ ARTRIDIL and TWYDIL¨ HIPPACAN+C. TWYDIL¨ LIQUID BLISTER has to be stopped 5 days before any competition. 26 HPH 06/07 ¨ sponsibility to check that the B) TWYDIL PRODUCTS QUESTIONS TO product complies with local regu- REQUIRING A DR YVES lations: in some cases, the local WITHDRAWAL PERIOD regulations forbid the provision BONNAIRE, of supplements to horses during a 1) TWYDIL¨ HIPPACAN+C Director of LCH certain period (generally 48 AND TWYDIL¨ ARTRIDIL hours) before any competition. Due to rapid scientific progress in HPH: Why are the eleutherosides control laboratories we must now in- and harpagosides now considered as 2) The certificates of control for the troduce a withdrawal period for com- prohibited substances? TWYDIL¨ products issued by plementary feeding stuffs containing YB: Eleutherosides and harpagosides LCH are based on the most se- eleutherosides and harpagosides. To substances with known therapeutic quali- vere controls anywhere in the avoid all possible risks, we undertook ties, consequently they act on one or world. But neither LCH nor a scientific trial with LCH to establish more body systems of the horse thus they naturally and by definition enter the TWYDIL¨ can be held responsi- the persistence of the eleutherosides ¨ category of prohibited substances as ble in relation to any particular or contained in TWYDIL HIP- described in the corresponding articles of original regulation valid in cer- PACAN+C and of harpagosides con- ¨ the international agreements (Trotting & tain countries for a certain disci- tained in TWYDIL ARTRIDIL. Galop). pline. We remember an example Based on the results of these tri- of a laboratory declaring a horse als, the withdrawal period for HPH: If the finding of a physiologi- positive for Vitamin E last year! TWYDIL¨ HIPPACAN+C and ¨ cal effect is sufficient for them to be It is the user’s responsibility to TWYDIL ARTRIDIL has been de- considered as doping substance, why assure that the formulation used termined as 48 hours before any not also consider water, oats and Vita- conforms to the regulations of the competition. min C as doping substances? specific competition involved. This delay is an official insurance YB: The problem does not arise for ¨ that TWYDIL HIPPACAN+C and water and oats (even vitamin C) which ¨ TWYDIL ARTRIDIL can be given are part of the normal feed of a horse, in full safety at the recommended consequently, a "normal” daily supply REMINDER OF THE quantities until 48 hours before any is not a problem. PRECAUTIONS competition. Note: this official guarantee is not A) OFFICIALLY CERTIFIED Practically, what do professionals valid for other feed supplements PRODUCTS CAN BE USED risk when they supply supplements con- containing the same substances un- WITHOUT RISK UP TO THE taining eleutherosides and harpago- less their persistence has been es- sides whose persistence has not been DAY OF COMPETITION tablished in a scientific study. tested? In France, France Galop, also YB: They risk using a product with a 1) THE TWYDIL¨ FEED guarantees that, even in case of con- higher concentration whose persistence SUPPLEMENTS trol during a training session, active may be greater. TWYDIL¨ validates every batch of substances of TWYDIL¨ HIP- production after analysis by LCH of PACAN+C and TWYDIL¨ AR- HPH: And this is not the case for the final product but also of the urine ¨ ¨ TRIDIL would not be considered as TWYDIL HIPPACAN+C and TWYDIL of a horse having received an overage a breach of the Racing Code, pro- ARTRIDIL ? of the product. viding their use has been admitted. YB: No, as we have used the same As with other feed supplements and formulations during our trials which 2) TWYDIL¨ COSMETICS vitamins, the French code does not showed clearly (with an important secu- Cosmetic products cannot be con- require a veterinary prescription for rity factor) that the molecules were not trolled by analysis of the product for feed additives containing extracts of no longer found in the urine and blood technical reasons, LCH validates harpagophytum and eleutherococ- of treated horses after 48 hours. every batch of production after cus. Consequently their use may be analysis of the urine and blood of a HPH: Many professionals fear to be mentioned by the trainer and not horse having received a large overage exposed to a positive control because of necessarily by the veterinarian. of the product. the presence of a contaminant even if this substance is found in infinitesimal 2) TWYDIL¨ LIQUID BLISTER ¨ quantity not having any physiological TWYDIL LIQUID BLISTER is a effect on the horse? product particularly containing YB: The sensitivity of our methods is camphor for horses with leg prob- generally adjusted according to the sub- lems. A withdrawal period of 5 days stances under investigation, an "infini- before any competition must be re- tesimal” quantity of feed contaminants spected.is a product particularly would not produce a positive result. containing camphor for horses with Only the substances exclusively leg problems. A withdrawal period "doping” are sought with the greatest of 5 days before any competition possible sensitivity. must be respected. 27 HPH 06/07 LIST OF CONTROLS REALISED BY LCH ON EACH BATCH OF PRODUCTION OF THE TWYDIL® PRODUCTS (certificates available at www.twydil.com) Each batch of final Urine of a horse having Blood of a horse having Until when is the product certified by received an overdose of received an overdose of product certified to be LCH not to contain any the batch, certified by the batch, certified by LCH used without any risk natural contaminants (1) LCH to be free of any to be free of any forbidden forbidden substances (2) substances (3) FEED SUPPLEMENTS TWYDIL® ARTRIDIL YES YES YES Until 48 hours before (on urine collected (on blood collected competition or doping 48 h after the last 48 h after the last control administration of administration of ARTRIDIL) (6) ARTRIDIL) (6) TWYDIL® CALMIN YES YES YES Day of the competition TWYDIL® COMPETITION YES YES YES Day of the competition TWYDIL® RACING YES YES YES Day of the competition TWYDIL® STUD YES YES YES Day of the competition TWYDIL® ELECTROLYTES YES YES YES Day of the competition Pails TWYDIL® ELECTROLYTES YES YES YES Day of the competition Mouth syringes TWYDIL® ELECTROLYTES+C YES YES YES Day of the competition TWYDIL® HEMATINIC YES YES YES Day of the competition TWYDIL® HEMOPAR YES YES YES Day of the competition TWYDIL® HIPPACAN+C YES YES YES Until 48 hours before (on urine collected (on blood collected competition or doping 48 h after the last 48 h after the last control administration of administration of HIPP+C) (7) HIPP+C) (7) TWYDIL® MINERAL COMPLEX YES YES YES Day of the competition TWYDIL® OMEGADIL YES YES YES Day of the competition TWYDIL® PMC YES YES YES Day of the competition TWYDIL® PROTECT PLUS YES YES YES Day of the competition TWYDIL® STOMACARE YES YES YES Day of the competition TWYDIL® STUD CAROTENE YES YES YES Day of the competition TWYDIL® TWYBLID YES YES YES Day of the competition TWYDIL® VIGORADE YES YES YES Day of the competition COSMETICS TWYDIL® 4LEGS NO (4) YES YES Day of the competition TWYDIL® LEG GEL NO (4) YES YES Day of the competition TWYDIL® HOOFCARE NO (4) YES YES Day of the competition TWYDIL® LEG PAINT NO (4) YES YES Day of the competition TWYDIL® LIQUID BLISTER NO (5) NO (5) NO (5) Until 5 days before competition or doping control (1) LCH certifies that the final product does not contain any of the 9 natural contaminants (caffeine, theobromine, theophyline, atropine, scopo- lamine, morphine, methylbufotenine, dimethyltriptamine, bufotenine) even at the limits of detection. (2) LCH certifies that the urine of a horse having received an overdose of the product does not contain any prohibited substances. (3) LCH certifies that the blood of a horse having received an overdose of the product does not contain any prohibited substances. (4) Due to the specific nature of the cosmetic products, LCH cannot control the product itself. However, LCH does control the urine and blood of a horse having been treated by these products during 3 days. (5) TWYDIL® LIQUID BLISTER (which will be withdrawn within the next months from the range of TWYDIL® products) contains camphor which is a prohibited substance. (6) Harpagophytum procumbens (one of TWYDIL® ARTRIDIL’s ingredients) henceforth is considered as a prohibited substance. LCH certifies that 48 hours after the last intake of TWYDIL® ARTRIDIL (with an overdose), the blood and urine of the horse do not contain any forbidden substance. (7) Eleutherococcus senticosus, Maxim. (one of TWYDIL® HIPPACAN+C’s ingredients) henceforth is considered as a prohibited substance. LCH certifies that 48 hours after the last intake of TWYDIL® HIPPACAN+C (with an overdose), the blood and urine of the horse do not contain any forbidden substance. 28 HPH 06/07 NEW TWYDIL® DIRECTOR OF PRODUCTION time. In 1993 he took over his par- ents’ farm which he now maintains as an organic farm with suckler cows. His hobbies are skiing in winter and climbing in summer; the latter he enjoys together with his daughter. With his wife he likes travelling to foreign countries. On the left: Ruedi Bernet, new TWYDIL® Director of Production. Ruedi Bernet joined TWYDIL¨ on In 1992, Ruedi Bernet continued 1 April 2006 to take responsibility his career as a sales representative for for the following departments: F.Hoffmann-La Roche Ltd in Basel, Ð purchasing manager of raw materi- selling vitamins, amino acids, trace als and packaging elements, enzymes and carotenoids Ðproduction manager of the entire in Switzerland and Austria. In this TWYDIL¨ product range position he came across TWYDIL¨ a Ð central stock and warehouse con- devoted customer of ROCHE’s high trol quality products for years. Through Ð sales in Germany, Switzerland and constant dialogue with TWYDIL¨ he Austria got to understand their needs and so could help them to constantly im- Professional career prove their products. In 2005 Ruedi Bernet went to Pan- Ruedi Bernet grew up on a farm in cosma Ltd, Geneva. where he en- Central Switzerland. He studied agri- larged his knowledge of flavours and culture, specialising in animal nutri- Climbing tour in his daughter’s company organic trace elements in animal nu- tion, at the Federal Technical Univer- trition. He has been able to utilize sity in Zurich where he obtained his this knowledge for the benefit of degree in 1979. Following his studies Age 53 TWYDIL¨. he managed the parental farm for When, in 2006, TWYDIL¨ was three years, working at the same time Civil status Married looking for a new production and as a part time teacher at an agricul- sales manager responsible for Ger- Nationality Swiss tural college. man speaking countries Ruedi Bernet In 1982 he was appointed as tech- didn’t hesitate to apply for the posi- Residence Buttisholz /Switzerland nical director for the cooperative tion since he had known the firm VLGZ (third biggest cooperative in since 1992 as the market leader for Diploma Degree in Agricultural Switzerland), where he was responsi- high quality horse products. Engineering / Animal ble for the production of 50,000 tons Nutrition of feedstuffs, minerals and special products. Alongside his responsibili- Languages German, French, ties he established the company Private life English Agrotronic Ltd, a firm specialising in Ruedi Bernet and his family live computer feeding systems for calves, near Lucerne in Central Switzer- Hobbies Skiing, climbing, cows and pigs. Very soon this firm land. The two adult children will travelling became the Swiss leader. complete their studies in 2 years 29 HPH 06/07 RESPIRATORY TROUBLES Dr Brieuc de Moffarts (DVM, Ms, PhD) TWYDIL¨ Research and Development Director The main factors limiting the can influence the necessary respira- When considering these factors it performance of sport horses are tory pressure to a considerable ex- is essential to differentiate between articulation difficulties (latest stud- tent. affecting the superior and the interior ies are demonstrated in this HPH The cause of respiratory patholo- air passages. It is necessary to recog- pages 4 to 8) and respiratory prob- gies with racing and competition nise that the damage at one level can lems. horses is often multifactorial. These negatively influence the other. For in- The output of maximum effort cre- factors include: ates a huge need for oxygen which puts the respiratory system under • particles floating in the air (dust, heavy stress. pollution, fungal spores….) Even the air flow is capable by it- • physical qualities of the air (tem- self of creating inflammation of the perature, humidity) trachea. • presence or absence of clinical vi- Only a small imbalance may be ral infections enough to harm the horse’s capacity • latent inflammation related to to breathe efficiently. So, for in- problems with the immune system stance, the variation of only one mil- • anti-oxidant imbalance limetre in the superior air passages • genetic and selection factors 30 HPH 06/07 Endoscopic pictures of the trachea. Left : accumulation of pus in trachea, right: accumulation of blood trachea. stance, the reduction of the diameter of the superior air passages can in- duce an increase in frequency and in- tensity of pulmonary bleeding. Here are two examples of prob- lems at each level found in active horses, 1) superior air passage : Ðswollen dorsal soft palate Ð haematogenous hemiplegia 2) lower air passage: Ð inflammation of small air pas- sages Ðbleeding due to exercise Preventive measures are first the se- lection of horses with no identifiable potential genetic troubles (haematogenous hemiplegia, ……) Then all possible hygienic mea- sures must be taken to limit the cre- ation of dust and ensure forage feed of premium quality. ANOTHER CONSIDERATION OF THE TWYDIL¨ TWYBLID FORMULA TWYDIL¨ has developed and reinforce the anti-viral de- TWYDIL¨ TWYBLID which fences. helps to reduce the risk factors TWYDIL¨ TWYBLID is a associated with inflammation of complementary feed supplement the inferior and superior air pas- which helps to control certain risk sages. factors related to problems of the The original formula of respiratory system. TWYDIL¨ TWYBLID, now re- TWYDIL¨ TWYBLID can be cently improved, contains vita- beneficially combined with mins, anti-oxidants and several TWYDIL¨ OMEGADIL whose bioflavonoids. TWYDIL¨ TWY- beneficial action on the capillary BLID helps to ensure the integrity blood circulation and on the gen- of capillary blood vessels, main- eral inflammatory balance may tain a good biochemical balance prove synergistic and beneficial. 31 HPH 06/07 THE WORLD’S BEST FOUR-IN-HAND DRIVER FELIX-MARIE BRASSEUR, TRIPLE WORLD CHAMPION Philippe Henry, Marketing Director TWYDIL¨ At 55 years old, Felix-Marie May I kindly ask you to pass in front the best horseman in France. Joining Brasseur has an impressive charisma. of me so that I can watch you at the Belgian, François Mathy, as a Meeting him, one understands work”. Half an hour later, the prince rider, Felix-Marie Brasseur broke in how tough this man is, winning the spoke again to the Belgian champion. "Gai Luron”, who, in 1976, won two last world championship in Aachen "Thank you, Monsieur Brasseur, for gold medals at the Olympic Games in in spite of 8,000 hostile German the best lesson of my life”. Montreal. spectators. "During the presentation Given so many compliments, Fe- In the meantime, the super talented of the medals, I thanked the specta- lix-Marie Brasseur reacts with mod- Brasseur, tried many other sports, tors as if nothing had happened and I esty: "The skill of my discipline con- such as skiing instructor or motocross was gratified to receive a standing sists simply in utilizing the horses’ rider even athletics and racing driver. ovation.” equilibrium to ensure that they are in "I participated in the 24 hours of Le For almost 15 years, this extraor- the best possible place”. Mans at the wheel of an old Porsche dinary champion has dominated the Son of a medical doctor, Felix- and I even passed the start/finish point four-in-hand obstacle competitions, Marie Brasseur started riding at 3 of the Boucles de Spa on the roof, hav- the supreme discipline. "I think that years old. After completing agricul- ing rolled several times”. there is a current that passes between tural studies and his military service Rider in jumping, eventing and in the horses and me. Maybe it is some in the cavalry, he returned to his pas- dressage, this daredevil collected kind of magnetism?” sion in the most demanding way: the many fractures before moving to har- Prince Philip, Duke of Edinburgh, horseman training school of Forêt de nessed teams, first as a judge and meeting him many years ago, spoke Rambouillet. "A school harder than then, for the first time in 1983, the to him quietly at a meeting of Gentle- the Foreign Legion”. Belgian Champion driver. A career is men Riders in Windsor Park in 1990. Felix-Marie Brasseur emerged as made of important encounters and "Monsieur Brasseur, I follow all major, and the Saumur Cavalry Brasseur is no exception. In 1987, the your competitions with great interest. School awarded him with the title of businessman, Jan de Clerck, asked 32 HPH 06/07 Brasseur to prepare four Lipizzaner for the World Championship the fol- lowing year. But the crucial move occurred in 1993 when Portuguese businessman, Manuel de Mello, persuaded Felix- Marie Brasseur to prepare a team of Lusitanian horses for the World Championship 8 months later. "A doubly foolish challenge as the Lusi- tanian’s were considered up to then as simple corrida horses and any- way, normally you need four years of training to get a team to competition level. However, I said to Mr de Mello - you are completely crazy but me too!” After his father and Maître Coul- land of Rambouillet, de Mello was the third key figure in Brasseur’s life. "A crucial encounter from the human as well as from the equine point of view”. Working with de Mello, the Bel- gian rider fell in love with Lusitanian horses, and all their many attributes. "For me it is the best horse in the world. I am fascinated by the beauty of its looks, its airiness and its artistic side which is not always appreciated at its real value by the judges”. And am doing nothing. You have to know at the last minute, I had to replace their faults? "A tendency sometimes that working with a team of four is the rear left horse with a small mare to be too vertical but this can be very long, slow and calm process. To who fulfilled its mission with perfec- changed through good training” ad- be successful it is necessary to show tion”. mits Felix-Marie Brasseur. calmness, logic and fairness. During In 1996, in his native Belgium, The four-in-hand turnout, the an- training hours, it is essential to be Brasseur realised his first great cient prerogative of kings, lives today absolutely consistent and always achievement by winning the Grand only thanks to some rich patrons "To make the same gestures”. Slam with the double title of World prepare a competitive team of four, Psychology is also essential. Champion and also by winning the you need about 300,000 Euros a "Even if I was almost killed by a World Cup. year, maintenance fees and a million horse, I would always show them I "The discipline has evolved very Euros to buy the right horses”. What am very happy with them, because I much. In the past, you had to pass is the best criterion for selection? "I never lose sight of the fact that they through a river on the straight. To- select nice looking horses a minimum are in charge”. day, they make you pass obstacles in 4 years old, who are receptive and A good four-in-hand horse must the water with four horses trailing find pleasure in teamwork." be capable of doing a lot of things by almost a ton”. Driving a pair is more a matter of itself. The competitions take place dur- speed with a direct influence by the "The best of all compliments is ing several days with an obstacle driver on the horses, whereas a team when I am told that my horses go all course asking for technique and of four is a scientist’s task requiring by themselves, without thinking that speed, a test of driven dressage, and hard work and perception. "In a team to arrive at that, thousands of hours of mobility. "In fact, having been a of four, the horses in front are 10 me- of work are needed”. racing car driver certainly helps in tres away from me. If I pull the reins Intelligent and unconventional, noticing the changes of track, and by 10 cm, this would have no effect. Brasseur, has brought new insights how to balance the centre of gravity. The only way to pass a message is the into the discipline. "Though every- I had also learnt that if you pass a driving whip or an order. If on the body struggles to work every horse turn at the limit, you have to raise the day of competition they do not obey, I for a specific place in the team, I, on accelerator foot at the approach of am lost”. the contrary, want all horses of the the next”. In this competition, the There is no other way to avoid this team to be interchangeable. This leader is helped by a groom, who is a issue than hard work. "I always rise makes them psychologically more bit like the co-pilot in a sidecar, lean- at 5 am and work 365 days a year. If adaptable and this approach saved ing into the turns to maintain the bal- you see me at work, you would think I me at the last World Games because, ance of the team. The groom may Ð 33 HPH 06/07 cionado of horses. A collector of car- 1952 : born in Belgium. riages at Huelva near Seville, Juan Andres Kifes dreamt for years of en- 1955 : started riding. tering a team of four at the World Championship. This is a new chal- 1987 : started driving four-in-hand. lenge for Brasseur who has to start 1993 : starts working with Lusitanian horses. all over again, this time with Andalu- sian geldings. "This is very exciting 1996 : Grand slam: double world champion in Waregem and world cup winner. but also intimidating” he confesses. At 55, Felix-Marie Brasseur, has 2000 : Individual bronze medal at Wolfsburg. known all the joys of world champi- 2006 : Individual gold medal at Aachen World Equestrian Games. onship but still dreams of a chal- lenge: to try a four-in-hand with Ara- bian horses. "I am also fascinated by Arabian horses and would like to try them in a within certain limits Ð intervene with "Those who use a horse to show-off four-in-hand. For this I would need certain technical problems. themselves or on the contrary, those to find horses capable of walking at 7 "I have such a relation of love and who present the horse”. km/hour drawing a load, to trot in confidence with my horses that some- Because he is inflexible in his ap- harness between 22 and 24 km/h, to times I have the impression that I can proach to his art, Felix-Marie have good airs and to have the ask anything from them. I had a stal- Brasseur has refused to allow his strength to pull the coach”. lion that cut his leg and could not be team of four, who won the World If you think you have Arabian anesthetized due to a coming compe- Championship in 2006, to be run as horses for sale answering these crite- tition. Believe it or not, I explained pairs in the 2007 World Champi- ria and if you would like to develop the situation to the horse. I told him, onship. In his opinion, this decision the image of Arabian thoroughbreds look old chap, the vet will come and would affect the chances of the team in a new discipline, you know who to he will hurt you a bit. You have to be in the World Games in Kentucky in call…. brave. I took his head in my arms and 2010. Consequently, he cancelled his "This would be a terribly exciting he did not wince even when the vet contract but did not stay long without challenge” he whispers as a stitched him up. The veterinary sur- a job. After Portugal, he moved to farewell, this champion who has geon has not yet recovered!”. Spain where he has been employed been appointed "Officier de l’Ordre Felix-Marie Brasseur classifies the by Juan Andres Kifes, a petrol refin- de la Couronne” by the King of professionals in two categories ery constructor and a great afi- Belgium. 34 TWYDIL®, THE POLYGLOT TWYDIL¨ DOCUMENTATION IS NOW TRANSLATED INTO 13 LANGUAGES. These catalogues are available at www.twydil.com or at request NOVÁ SKUPINA VÝROBKŮ 4 NEWS THE NEW RANGE OF PRODUCTS The Swiss equine products of superior quality Švýcarský vyvážený výrobek vynikající kvality . UNSERE NEUE TWYDIL® YENI ˘ PRODUKTEPALETTE 4 NOUVEAUTÉS ÜRÜN KATALOGU LA NOUVELLE GAMME DE PRODUITS . Schweizer Pferdeprodukte von höchster Qualität La garantie Suisse d’une gamme de qualité supérieure. Ustün kaliteli yaris, ati ürünlerinde Isviçre garantisi. NOVA GAMA LA NUEVA LINEA NOWY ASORTYMENT ® PROIZVODA 4 NUOVI DE TWYDIL PRODUKTÓW PRODOTTI LA NUOVA LINEA DI PRODOTTI Švajcarski proizvodi za konje vrhunskog kvaliteta Prodotti svizzeri di qualità superiore per equini PRODUCTO SUIZO DE ALTA CALIDAD PARA CABALLOS DE DEPORTES Najwy˝szej jakoÊci szwajcarskie produkty dla koni 4 nieuwigheden ONZE NIEUWE PRODUCTENGAMMA Zwitserse kwaliteitsproducten voor paarden Other translations are under development. 36 ? 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