RESEARCH ARTICLE Genetic Diversity and Phylogenetic Evolution of Tibetan Sheep Based on mtDNA D-Loop Sequences Jianbin Liu1,2☯‡*, Xuezhi Ding1☯‡, Yufeng Zeng1☯‡, Yaojing Yue1,2, Xian Guo1, Tingting Guo1,2, Min Chu1, Fan Wang3, Jilong Han1,2, Ruilin Feng1,2, Xiaoping Sun1,2, Chune Niu1,2, Bohui Yang1,2, Jian Guo1,2, Chao Yuan1,2 1 Lanzhou Institute of Husbandry and Pharmaceutical Sciences of the Chinese Academy of Agricultural Sciences, Jiangouyan Street, Lanzhou, China, 2 Sheep Breeding Engineering Technology Research Center of Chinese Academy of Agricultural Sciences, Jiangouyan Street, Lanzhou, China, 3 China Agricultural a11111 Veterinarian Biology Science and Technology Co. Ltd, Xujiaping, Lanzhou, China ☯ These authors contributed equally to this work. ‡ These authors are co-first authors on this work. *
[email protected] OPEN ACCESS Abstract Citation: Liu J, Ding X, Zeng Y, Yue Y, Guo X, Guo T, The molecular and population genetic evidence of the phylogenetic status of the Tibetan et al. (2016) Genetic Diversity and Phylogenetic ’ Evolution of Tibetan Sheep Based on mtDNA D-Loop sheep (Ovis aries) is not well understood, and little is known about this species genetic Sequences. PLoS ONE 11(7): e0159308. diversity. This knowledge gap is partly due to the difficulty of sample collection. This is the doi:10.1371/journal.pone.0159308 first work to address this question. Here, the genetic diversity and phylogenetic relationship Editor: Bi-Song Yue, Sichuan University, CHINA of 636 individual Tibetan sheep from fifteen populations were assessed using 642 complete Received: January 28, 2016 sequences of the mitochondrial DNA D-loop. Samples were collected from the Qinghai- Tibetan Plateau area in China, and reference data were obtained from the six reference Accepted: June 30, 2016 breed sequences available in GenBank.