D.A. Enikeyev, E.N. Khisamov, S.A. Enikeyeva, I.T. Idrisova FREE RADICAL OXIDATION IN THE BLOOD SYSTEM CHEMICAL POLLUTION ENVIRONMENT Hannover 2014 Sonderdruck aus Hannoversches Jahrbuch Band 2, 2014 D.A. Enikeyev E.N. Khisamov S.A. Enikeyeva I.T. Idrisova Free radical oxidation in the blood system chemical pollution environment Sonderdruck aus Hannoversches Jahrbuch Band 2, 2014 Serie: Medizin Unter Förderungen der Europäischen Akademie der Naturwissenschaften e.V. Hannover 2014 1 Europäische Akademie der Naturwissenschaften e.V. medical sciences UDC 621.1.54.111 Free radical oxidation in the blood system chemical pollution environment D.A. Enikeyev, E.N. Khisamov, S.A. Enikeyeva, I.T. Idrisova GOU VPO «Bashkir State Medical University, Ministry of Health of Russia», Ufa, Russia ( 450000, Ufa, st. Lenin 1; email: Hisamov7958 @ yandex.ru) Occupation: pathophysiology 140303 . A study of rabbit blood chemiluminescence contained towns and rural areas of the Republic of Bashkortostan ( RB) . Assessing the level of free radical oxidation was conducted in terms of the light sum (S) and the maximum luminescence (J). Received negative indicators in the urban environment . Comparative study showed declines CL AFO process of phagocytosis of white blood cells and red blood cells increase in lipid peroxidation and serum of rabbits kept in the cities in terms of chemical pollution. Was investigated in parallel character of individual adaptation to chemical factors of low intensity environment in terms of FRO blood. For stays animals brought from the countryside to cities , industrial centers of RB for three months were marked by phase states - signs of adaptation and subsequent deadaptatsii , particularly depression of phagocytic activity and increased lipid peroxidation of blood, refl ecting uncoupling of oxidative phosphorylation. Keywords: blood chemiluminescence, town, village . 2 Sonderdruck aus Hannoversches Jahrbuch Band 2, 2014 Free radical oxidation in the blood system chemical pollution environment Relevance Contamination of the environment creates new challenges for the biotic environment. In particular, in the industrial centers of the Republic of Bashkortostan (RB) recorded a signifi cant chemical pollution. [4]. Complex eff ects of negative factors of low intensity can adequately assessed only in the process of biological monitoring. The aim of the present work was put on the establishment of the mammalian response eff ect of chemical pollutants in RB. A comparative analysis of blood parameters of rabbits are in urban and rural areas in a comparative perspective. Research methods The material was analyzed in adult chinchilla rabbits. A comparative analysis of free radical oxidation (FRO) of blood in animals fi nding in urban and rural areas in a comparative perspective. In order to establish the nature of adaptation to adverse environmental factors have been made dynamic observation of the animals brought from rural to urban conditions for 3 months. State of free radical oxidation (FRO) in the blood was investigated using biochemiluminometer BKL 06, wherein the detector was installed lights photomultiplier PV 000.335.557.TU. At the same time studied the oxidation of lipids (LPO) and red blood cell mass, (LPO) serum and chemiluminescence (CL) caused by the release of reactive oxygen species (FRO) during phagocytosis. Assessing the level of free radical oxidation was conducted in terms of the light sum (S) and the maximum luminescence (J) [1,6]. Statistical data processing was carried out under the program statistics MO Excel (defi nition of signifi cant diff erences in the t -Studentt-test). [9]. Results and discussion Application of chemiluminescence (CL) in the study of hematological parameters diff erentially expanded opportunities to evaluate the nature of the adaptive response of the organism under the infl uence of chemical factors of low-intensity environment. View of the state of FRO in the blood system was compiled on the basis of CL intensity of ROS during phagocytosis of red blood cells, as well as lipid peroxidation in erythrocytes and serum. FRO study was conducted in a relatively environmentally friendly zone - in 3 Europäische Akademie der Naturwissenschaften e.V. Gorny Chishminsky district as well as in the temperate zone of contamination - the village „Ceramics workshop“ Annunciation district and cities with heavy chemical pollution of the environment – Belebey, Ufa, Ishimbay [4]. Thus, the average values of the light sum for 300 seconds. Lumino initiated chemiluminescence of reactive oxygen species (ROS) during phagocytosis in rabbits fi nding in Gorny Chishminsky area was 31.2 x 102 impulses. Amplitude maximum luminescence average equaled 26.9 pulses / sec. Obtained in this kinetic curves of CL had a gentle nature. For example, the rabbit N 3 slope of the curve changes in light intensity corresponded with an increase in radiation - 2.6 E - 0:2 and recession - 3.2 E - 0.2 C. Research parameters initiated luminol chemiluminescence in the AFO tsiolitom stimulated phagocytosis in rabbits in the village. Gorny Chishminsky district showed that the average values of the light sum for 300 seconds. accounted for 35.9 x 102 impulses amplitude maximum luminescence - 24.3 pulses / sec. Kinetic curves were similar with those observed in the experiments without stimulation of phagocytosis tsiolitom. Parallel study of the AFO CL during phagocytosis of peripheral blood cells of rabbits contained in other regions of Belarus, revealed signifi cant diff erences compared with data from Gorny Chishminsky district. For ease of comparison, fi gures CL Gorny animals were taken as 100%. Thus, data AFO CL process of phagocytosis of red blood cells contained in the village. „Pottery workshop“ Annunciation area were signifi cantly lower than those in Gorny Chishminsky district. Average value lightsum 300 sec. initiated luminol chemiluminescence ROS during phagocytosis without stimulation equaled 23.1 x 102 impulses, representing 74.2 % of respective animals in Gorny soderzhavschihsya Chishminsky area (P < 0.05). The average value of the amplitude of the maximum luminescence corresponded to 19.5 pulses / sec. - 72.8 % (P < 0.05). Directly proportional shifts were identifi ed and the process of phagocytosis stimulation tsiolitom. Average values lightsum within 300 seconds. x 102 impulses were 27.1 (75.5 %) (P < 0.05). and the amplitude of the maximum illumination - 17.7 pulses / sec (72.8%) (P < 0.05). Consequently, aggregates AFO CL initiated the process of phagocytosis without stimulation and with stimulation indicates a weakening of cellular immunity in animals kept in the village. „ Ceramic workshop „ Annunciation district. AFO CL study phagocytosis of red blood cells in rabbits in fi nding in BELEBEY revealed a reduction of the amplitude maximum luminescence and light sum compared with those of animals in Gorny Chishminsky district. Thus, the light sum for 4 Sonderdruck aus Hannoversches Jahrbuch Band 2, 2014 300 seconds. luminol chemiluminescence initiated by ROS phagocytosis without stimulation average equals 22.1 impulses x 102 (71.1 %) (P < 0.05) and the maximum amplitude of the luminescence - 18.8 imp / s (69.9 %) (P < 0.05). These indicators initiated luminol chemiluminescence AFO process of phagocytosis stimulated tsiolitom respectively equaled 28.4 x 102 impulses (79.2%) (P <0.05) and 18.9 impulses / sec (78.1%) (P <0.05). Consequently, in Belebey also was a decrease in phagocytic function from the peripheral blood leukocytes. The rabbits were kept in Ufa, AFO CL process of phagocytosis of blood cells revealed a further decline in cellular immunity. Thus, the average value of the light sum for 300 seconds. initiated luminol chemiluminescence AFO not stimulated phagocytosis equaled 19.6 x 102 impulses (62.9%) (P <0.05), and the amplitude of the maximum emission was 17.0 impulses / sec (63.4%) (P <0.05). These parameters initiated chemiluminescence AFO process tsiolitom stimulated phagocytosis average equaled 21.4 x 102 impulses respectively (59.8 %) (P < 0.05) and 15.0 imp / sec (61.9 %) (P < 0, 05). About the same level of changes in the phagocytic function of blood cells were observed in animals in fi nding in Ishimbay. Luminol CL light sum initiated by AFO during phagocytosis without stimulation averaged 19.0 x 102 impulses 300 per sec. (61.1 %) (P < 0.05) and the maximum amplitude of the luminescence - 16.1 imp / s (59.9 %) (P < 0.05). The average value of the described indicators when stimulated phagocytosis tsiolitom equal respectively 21.9 h102 pulses (61.1%) (P <0.05) and 15.1 pulses / sec (62.1 %) (P < 0.05). Kinetic curves of CL initiated by AFO phagocytosis with stimulation and without it had roughly the same type of character. For example, in the rabbit under N 2 contained in Belebey, the value of the slope of the curve changes in light intensity radiation corresponded with an increase in E 5.1 - 0.2, while the decline was 2.4 E - 0.2. Thus, the study states in animals that were in the regions of RB, with varying degrees of chemical contamination by AFO CL initiated the process of phagocytosis and stimulated without stimulation showed a mixed picture. The most favorable level of cellular immunity was observed in animals kept in Gorny Chishminsky district. Several depression was found in rabbits that were in the village. „Pottery workshop„ and Belebey. Relatively lower rates of phagocytic function of blood cells observed in animals kept in the cities. Ufa, Ishimbai (table 1, Figure 1). Bactericidal eff ect of elevated concentrations of FRO products used by phagocytes (leukocytes, tissue macrophages) for primary „ oxidative attack„ 5 Europäische Akademie der Naturwissenschaften e.V. on alien membrane of bacteria, viruses, and then these FROs damaged membranes can be disassembled second acting factor - lysosomal hydrolytic enzymes. During phagocytosis has been increasing (explosion) consumption and activation of FROs. Phagocytes at the place of contact with the membrane in the opponent‘s cells begin to conduct an intensive one-electron reduction of oxygen in the electron transport chain at the level of oxidases ksantooksidazy, aldehyde.