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ISSN 2035-4088 Proceedings of the 6th International Symposium on the Mediterranean Pig October 11 – 13, 2007 Messina - Capo d’Orlando (ME), Italy Editors L. Nanni Costa, P. Zambonelli, V. Russo TABLE OF CONTENTS Foreword ………………….………………………………………………...………..… 8 SESSION 1. IMPROVEMENT AND MANAGEMENT OF THE GENETIC RESOURCES L. Ollivier. Analyses of the European pig diversity using genetic markers ………………..… 10 A.Fernández, J. Rodrigáñez, C. Rodríguez, and L. Silió. Heterosis for litter size of Iberian sows is limited to second and later parities .… 23 A. Fernández, J.M. García-Casco, and L. Silió. Genetic trends for growth of young pigs in two breeding nuclei of Iberian breed .. 27 L. J. Royo, I. Álvarez, I. Fernández, L. Pérez-Pardal, A. Álvarez-Sevilla, J. Santos e Silva, R. Godinho, N. Ferrand, and F. Goyache Genetic characterisation of Celtic-Iberian pig breeds using microsatellites ......… 31 A. Crovetti, R. Bozzi, L. Nardi, M. Gallo, L. Buttazzoni, and O. Franci Pedigree analysis of Cinta Senese breed ……………………………………….…. 35 R. Bozzi, A. Crovetti, L. Nardi, C. Pugliese, F. Sirtori, and O. Franci Study of genes related to meat quality in Cinta Senese pig breed …………...…. 41 R. Davoli, P. Zambonelli, M. San-Cristobal, E. Scotti, L. Fontanesi, M. Colombo, S. Dall'Olio, S. Braglia, V. Russo Snps and microsatellite markers analysis for genetic diversity study in Italian pig breeds …………………………………………………………………………….... 46 D’Alessandro E., Fontanesi L., Liotta L., Scotti E., Davoli R., Chiofalo V., Russo V. Genetic analysis of the KIT and MC1R coat colour affecting genes in the Nero Siciliano pig breed and perspectives on the use of mutations at these two loci for breed traceability ……………………………………………………………..…… 54 D. Matassino, N. Castellano, D. Falasca, D. Fornataro, P. Petrillo, C. Rossetti, E. Cristofaro, G. Varricchio Variation of micronuclei frequency in lymphocytes of ancient ‘Casertana’ autochthonous pig genetic type (AAGT) in relation to photoperiod. Preliminary results ………………………………………………………………..…… 60 D. Matassino, S. Bordonaro, N. Castellano, A. M. Guastella, C. Incoronato, F. Monaco, M. Occidente, F. Pane, C.M.A. Barone CRC locus screening in some Italian pig ancient autochthonous genetic types (AAGTs). Preliminary results ……………………………………………………....... 68 Proceedings of 6th International Symposium on the Mediterranean Pig 1 SESSION 2. SANITARY APPROACHES IN THE FARMING SYSTEMS G.Poglayen, S. Giannetto, A. Scala, M.T. Manfredi, L. Rinaldi, B. Marchesi Pig parasites: a short Italian history ………………………………………..……….. 75 L. Battistacci, M. Sensi, M. Timi, S. Marchi, L. Moscati Adaptation ability of natural immune system in cinta senese swine and commercial hybrid breeding pigs to outdoor rearing …………………………...…. 82 M. Sensi, L. Moscati, S. Costarelli, C. Mariotti, R. Ciappelloni, L. Battistacci Ideal approach to health management in an outdoor pig breeding farm: a case study on the Umbrian appennine ……………………………………...……. 85 G. Lanteri, F. Marino, G. Mazzullo, D. Macrì Nero Siciliano pig. A model for the study of unusual pathologies ……………..… 89 C. Richomme, F. Casabianca, O. Maestrini, C. Ducrot, P. Boireau Trichinellosis and extensive farming system: an eco-epidemiological approach of the sanitary situation in insular area of Corsica ………….…………. 93 A. Scala, G. Sanna, A. Varcasia, R. Marrosu, G. Cocco, A.P. Pipia, L. Polinas, B. Tanda, G. Garippa Isosporiasis in domestic pigs of Sardinia (Italy) …………………………………… 98 A. Varcasia, G. Tosciri, T. Pedes, A.P. Pipia, R. Marrosu, A. Scala, G. Garippa Cystic echinococcosis in pigs and wild boars of Sardinia (Italy) …….….………. 102 E. Brianti, G. Gaglio, M. Ferlazzo, S. Abbene, G. Poglayen, S. Giannetto A review of parasites found in the Sicilian Black pig …………………...………… 105 G. Gaglio, E. Brianti, A.L. Risitano, G. Poglayen, S. Giannetto Ectoparasitoses in pigs slaughtered in Sicily: prevalence and diagnostic tools………………………………………………………………………… 108 SESSION 3. FEEDING AND REARING SYSTEMS B. Lebret Effects of feeding and rearing systems on growth, carcass traits and meat quality in pigs ……………………………………………………………….………… 113 B. Šegula, M. Škrlep, D. Škorjanc, M. Čandek-Potokar Preliminary results on extensive free range rearing of pigs with access to pasture indicate inferior meat quality and improved fatty acids composition …. 127 J. Tirapicos Nunes, J.Neves, L. Campaniço; H.Santos Trial to study possible effect of acorns source (green oaks vs cork oaks) on traditional pork products ……………………………………………………………. 134 Proceedings of 6th International Symposium on the Mediterranean Pig 2 R. Charneca, J. Tirapicos Nunes, J. Le Dividich Comparative study on colostrum production and colostrum composition in Alentejano swine breed and LWxLR sows – preliminary results ……….……….. 138 L. Moscati, L. Battistacci, S. Orsini, M. Sensi Organic breeding herd: managerial difficulties in order to satisfy the requirements of the lactation sows …………………………………………………. 144 J. Robledo, J. D. Vargas, F.González, L. Prieto, J. A. Andradas, M. A. Aparicio Animal welfare and production in the Iberian Pig …………………………………. 146 F.González, J. D.Vargas, J. Robledo, L. Prieto, J. A. Andrada, M. A. Aparicio Influence of environmental conditions in Iberian pig rearing systems …..……… 153 J. Neves, A.B. Freitas, J.M. Martins, M.I. Ferraz de Oliveira, M. Cancela d’Abreu Evolution of the composition in fatty acids of the dorsal subcutaneous adipose tissue from Alentejano pigs fed on pasture and acorns ……………..…. 161 J. Neves, A Freitas, J. Martins, J. Nunes Alpha-tocopherol content on the semimembranosus muscle of Alentejano pigs reared in intensive and extensive conditions ………………………...……………. 164 L. Giuliotti , J. Goracci , M. N. Benvenuti, A. Acciaioli, G. Campodoni Effect of pasture on meat and fat quality in Cinta Senese pigs ……………...….. 168 M. Bonanzinga, F. Cappè, G. Nardi The "Cinta Senese" breeding system: production system, breeding strategies and perspectives of valorization …………..…..………………………………………… 172 A. Zumbo, A.R. Di Rosa, V. Di Marco, V. Aronica, M. Russo, V. Pruiti In vitam and post mortem performances of “Nero Siciliano”fattening pigs fed with different diets …………...……………………………………………………….. 179 B. Chiofalo, L. Liotta, L.Sanzarello, D. Piccolo, V. Chiofalo Vitamin E administration in Nero Siciliano pigs: Effet on the oxidative stability of “Nebrodi” cured sausage ………….………………………………………………… 183 L. Liotta, B. Chiofalo, E. Scinardo Tenghi, V. Chiofalo Dietary supplementation of rosemary extract in growing Nero Siciliano pigs: effect on some haematological parameters ……………………………………….. 187 L. Liotta, G. Madonia, E. D’Alessandro, A. D’Amico, M. Cavallaro, V. Chiofalo Performances of Nero Siciliano sows and lactating piglets …………..…………. 191 A. Zumbo, A.R. Di Rosa, V. Lo Presti, V. Pruiti, D. Piccolo analysis of the volatile composition of fresh and seasoned lard in “Nero Siciliano” pigs in relation of fattening diet ………………………………...…………………… 194 Proceedings of 6th International Symposium on the Mediterranean Pig 3 A. Zumbo, C. Pugliese, V. Lo Presti, F. Sirtori, D. Piccolo, A. R. Di Rosa Effect of fattening diets on the volatile flavour compound of “Cinta Senese” salami and seasoned lard ..............................................................................…. 198 A. Acciaioli, F. Sirtori, L. Pianaccioli, S. D’Adorante, S. Parenti Replacement of soybean with Vicia Faba and Pisum Sativum in the growht - fattening of Cinta Senese pig …………………………………..…………………… 203 D. Karolyi, Z. Luković, K. Salajpal Production traits of Black Slavonian pigs ………………………………………..… 207 A. Rossi, P. Ferrari, M. B. Bossio, F. Monaco, A. Fusaro Growth performance and meat quality of outdoor reared Calabrese pigs …..…. 214 A. Zumbo, V. Lo Presti, A. R. Di Rosa, V. Pruiti, V. Di Marco, D. Piccolo Fatty acid profile of intramuscular fat of “Nero Siciliano”fattening pigs fed with different diets ……….………………………………………………………………… 224 A.R. Di Rosa, R. Ordile, V. Di Marco, M. Pagliaro, L. Sanzarello, A. Zumbo Physical and chemical traits of meat of “Nero Siciliano” fattening pigs fed with different diets ……..………………………………………………………………..…. 228 B. Chiofalo, R. Costa, L. Mondello, V. Chiofalo Nero Siciliano pig for the production of “Nebrodi” cured sausage: Effect of some traditional diets on sensorial characteristics ………...…………………………….. 232 M. I. Ferraz de Oliveira, M. Cancela d´Abreu, A. Freitas The effect of Polyethylene Glycol (Peg) on protein output of free range Alentejano pigs …………..…………………………………………………………… 236 SESSION 4. QUALITY OF MEAT AND MEAT PRODUCTS I. Muñoz, J. Arnau, P. Gou, P. Picouet New methods to assess and improve the quality of Mediterranean dry-cured hams …….…………………………………………………………………………….. 241 M. Prevolnik, M. Čandek-Potokar, M. Novič, D. Škorjanc An attempt to predict meat drip loss by means of artificial neural networks …… 249 E. Berni, C. Cacchioli, C. Diaferia, E. Spotti Microbial surface colonization in Nebrodi salame ………………………………... 253 J. Gonzalez, M. Gispert, P. Rodríguez, M. Gil, J. Jaume, J. Tibau, M.A. Oliver Carcass and meat quality of porc negre Mallorquí (Majorcan Black Pig) ……… 258 G. Pirone, C. Diaferia, T. Iaccarino, G. Madonia, V. Demarco, V. Pruiti Ripening techniques and microbiological characteristics of Nebrodi salame …. 262 Proceedings of 6th International Symposium on the Mediterranean Pig 4 R. Morales, J. Arnau, X. Serra, & P. Gou Meat pH influence on texture of dry-cured ham with reduced salt content and submitted to different storage temperatures ………….…………………………… 267 B. Martínez, B. Rubio, C. González-Fernández, D. 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  • Identification of a Coat Colour Gene Marker in Cinta Senese

    Identification of a Coat Colour Gene Marker in Cinta Senese

    1 Authentication of “mono-breed” pork products: identification of a coat colour gene marker in 2 Cinta Senese pigs useful to this purpose 3 4 L. Fontanesi a,*, E. Scottia , M. Gallob, L. Nanni Costaa, S. Dall’Olioa 5 6 7 a Department of Agricultural and Food Sciences, Division of Animal Sciences, University of 8 Bologna, Viale Fanin 46, 40127 Bologna, Italy 9 b Associazione Nazionale Allevatori Suini (ANAS), Via L. Spallanzani 4, 00161 Roma, Italy 10 11 * Corresponding author: tel. +39 051 2096535 12 E-mail: [email protected] (L. Fontanesi) 13 14 Running title: A DNA marker useful for pork product authentication 15 1 16 Highlights 17 Cinta Senese is a local Italian pig breed with a belted coat colour 18 The value of its meat production chain should be protected from frauds 19 We analyzed the KIT gene to identify a suitable DNA marker for this purpose 20 A single nucleotide polymorphism (g.43597545C>T) was almost fixed in Cinta Senese 21 Probability to correctly assign an unknown meat sample to Cinta Senese was ~1.00. 2 22 Abstract 23 The possibility to authenticate food products is crucial to defend local livestock production chains 24 from frauds. Cinta Senese is an autochthonous pig breed reared under extensive or semi-extensive 25 management systems, mainly in the Tuscany (Italy). A Protected Designation of Origin (PDO) 26 brand for Cinta Senese meat was recently obtained. The breed is characterised by a typical black 27 with a white-belted coat colour pattern. We analyzed a coat colour gene (KIT) to identify a DNA 28 marker that could be useful for Cinta Senese meat product authentication.
  • The Exclusiveness of Privacy

    The Exclusiveness of Privacy

    ANCIENT FARMHOUSE IN THE WOODS. THE EXCLUSIVENESS OF PRIVACY A villa with 7 hectares of land, all surrounded by further hundreds of hectares of forest: The castle of Celsa and the Villa Cetinale are nothing but the homes of your neighbors. Nature and history close to the wonderful city of Siena. Among the many fantastic farmhouses in the green hills of the province of Siena, this is certainly the most exclusive, not for luxury or refinement, but simply thanks to the nature that surrounds it and for the discretion that guarantees. Just looking at the satellite photos it’s clear how this property, surrounded by greenery, enjoys peace, intimacy, absolute privacy. But in spite of his intimacy, this location so reserved doesn’t means that property is far from everything. The characteristic villages of Ancaiano, Santa Colomba, Carpineto are less than 2 km away and are reachable in five minutes using good roads. The Municipality of Sovicille is less than 4 km away, the wonderful ancient village of Monteriggioni, or D'Else valley are only 9 km away.Also Siena is very close and can be reached in less than 20 minutes. The property borders Villa Cetinale, a magnificent residence whose baroque landscaped garden is one of the most important in Italy. It was built in 1670 by Cardinal Flavio Chigi and is now owned by an English parliamentarian of the House of Lords who after restoring it also allows the visit to the public. A little further there is the wonderful Celsa Castle, dating back to the thirteenth century, also rich in history, charm and secrets.
  • Animal Genetic Resources Branch, FAO S

    Animal Genetic Resources Branch, FAO S

    Editor-in-Chief Guest-Editor The designations employed and the presentation of material in this information product do R. Baumung C. Ligda Editorial Advisory Board not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations (FAO) concerning the legal or development Editors status of any country, territory, city or area or of its authorities, or concerning the delimi- Editor-in-Chief: R. Baumung, Animal Genetic Resources Branch, FAO S. Galal; I. Hoffmann tation of its frontiers or boundaries. The mention of specific companies or products of manufacturers, whether or not these have been patented, does not imply that these have Editors: S. Galal; I. Hoffmann been endorsed or recommended by FAO in preference to others of a similar nature that Animal Genetic Resources is an international journal pub- are not mentioned. lished under the auspices of the Animal Genetic Resources Editorial Board: L. Alderson The views expressed in this information product are those of the author(s) and do not Branch of the Animal Production and Health Division, Food J.S. Barker necessarily reflect the views or policies of FAO. and Agriculture Organization of the United Nations (FAO). I. Curik Ressources génétiques animales est un journal interna- H. Jianlin Les appellations employées dans ce produit d’information et la présentation des données tional publié sous les auspices de la Sous-Division des qui y figurent n’impliquent de la part de l’Organisation des Nations Unies pour l’alimenta- ressources génétiques animales de la Division de la produc- J.