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Framingham Heart Study 73 Mt Framingham Heart Study 73 Mt. Wayte Avenue Framingham, MA 01702 Laboratory data - method manual dataset name= l_urmtbl_ex06_1_0742s Analyte(s) measured Urine Panel (Metabolomics and CKD) Units none (Integrated LC-MS peak areas) Laboratory information Laboratory name Broad Institute of MIT and Harvard Location 7 Cambridge Center, Cambridge, MA 02142 Contact name Contact email Other contacts Contact email Sample information Offspring exam 6, This was a nested case control study of 386 individuals. All participants were free of CKD at baseline. Half developed CKD at follow- up (exam 8); cases and controls were age-sex Cohort / Exam number matched Sample type Urine Number of samples N=386; case-control [Incident CKD between exams 6 and 8] Methodology Method Type LC-MS: CHM or HILIC Assay characteristics Number of measurements (single/duplicate/other design) 10% in duplicate; randomly chosen Inter-assay CV See worksheet Intra- Inter-assay CVs Intra-assay CV See worksheet Intra- Inter-assay CVs Run dates Sept - Dec 2012 Framingham Heart Study Offspring exam 6 - Urine panel Fox/Broad Intra- and Inter-assay CVs 2/4/2013 Variable Method Intra-assay CV N Inter-assay CV N 2-aminoadipate CMH 9.8 15 10.5 23 3-hydroxykynurenate CMH 10.6 15 13.7 23 3-phosphoglycerate CMH 8.2 15 10.5 23 alpha-glycerophosphate CMH 7.2 15 7.0 23 4-pyridoxate CMH 10.9 15 17.9 23 aconitate CMH 12.6 15 9.3 23 adenine CMH 9.4 15 14.0 23 adipate CMH 10.5 15 12.5 23 AMP CMH 21.7 15 18.4 23 ADP CMH 23.2 14 22.2 21 cAMP CMH 8.2 15 6.9 23 citrate CMH 7.7 15 6.8 23 isocitrate CMH 9.4 15 12.5 23 CMP CMH 13.2 14 17.5 20 cystathionine CMH 8.7 14 7.5 22 cytidine CMH 8.2 15 6.9 23 erythrose-4-phosphate CMH 10.2 15 12.0 23 F1P/F6P/G1P/G6P CMH 16.9 15 13.0 23 fructose/glucose/galactose CMH 5.9 15 5.0 23 fumarate/maleate/alpha-ketoisovalerate CMH 12.8 15 18.7 23 glucuronate CMH 11.5 15 11.5 23 guanine CMH 9.6 15 11.5 23 guanosine CMH 7.6 14 11.2 23 hippurate CMH 5 15 6.2 23 homocystine CMH 24 14 23.5 22 homovanillate CMH 11 15 11.3 23 hypoxanthine CMH 6.9 15 8.6 23 inosine CMH 8 15 9.0 23 kynurenine CMH 11.2 15 14.2 23 lactate CMH 13 15 15.8 23 lactose CMH 8.9 15 8.0 23 malate CMH 7.9 15 9.4 23 orotate CMH 14.4 15 15.5 23 oxalate CMH 8.8 15 14.0 23 pantothenate CMH 10.8 15 11.1 23 phosphotyrosine CMH 18.1 15 17.8 21 propionate CMH 20.8 14 20.3 23 quinolinate CMH 11.5 15 15.2 23 salicylurate CMH 24.1 15 34.3 22 sorbitol CMH 5.1 15 3.6 23 succinate CMH 7.3 15 10.4 23 methylmalonate CMH 12.5 15 17.6 23 sucrose CMH 7.8 15 6.1 23 thymine CMH 8.7 15 7.5 23 uracil CMH 6.5 15 6.3 23 urate CMH 5.6 15 5.4 23 uridine CMH 6.8 15 5.2 23 xanthine CMH 10.4 15 12.8 23 xanthurenate CMH 9.1 15 11.1 23 cholate CMH 22.1 13 25.8 20 glycocholate CMH 10.5 15 7.3 23 taurocholate CMH 15.8 15 21.1 23 glycodeoxycholate/glycochenodeoxycholate CMH 9.4 15 10.4 23 taurodeoxycholate/taurochenodeoxycholate CMH 15 15 32.7 23 suberate CMH 9.6 15 10.2 23 hydroxyphenylacetate CMH 8.9 15 9.2 23 indoxylsulfate CMH 9.7 15 7.6 23 indole-3-propionate CMH 9.3 15 10.1 23 gentistate CMH 9.6 15 7.6 23 3-methyladipate-pimelate CMH 10.8 15 9.0 23 FMN CMH 28.9 15 38.9 23 Framingham Heart Study Offspring exam 6 - Urine panel Fox/Broad Intra- and Inter-assay CVs 2/4/2013 VMA CMH 10.2 15 7.8 23 alpha-hydroxybutyrate CMH 7.8 15 14.2 23 beta-hydroxybutyrate CMH 10.1 15 12.8 23 2-hydroxyglutarate CMH 9.7 15 12.6 23 inositol CMH 9.7 15 9.2 23 4-hydroxybenzaldehyde CMH 20.5 14 20.8 22 phosphocreatine CMH 22.1 3 30.5 7 benzoate CMH 30.4 6 27.9 10 indolelactate CMH 27.7 10 28.2 10 homogentisate CMH 18.6 12 24.2 15 PEP CMH 27.9 13 29.5 17 glycine HILIC 5.7 16 5.5 22 alanine HILIC 4.7 16 6.1 22 serine HILIC 3.7 16 6.2 22 threonine HILIC 5.6 16 7.2 22 methionine HILIC 9.1 13 14.8 22 glutamate HILIC 5.4 16 6.3 22 asparagine HILIC 4.5 16 4.9 22 glutamine HILIC 3.4 16 4.6 22 histidine HILIC 7.4 16 6.6 22 arginine HILIC 6.3 16 6.2 22 lysine HILIC 7.2 16 6.4 22 valine HILIC 2.5 16 4.6 22 leucine HILIC 3.4 16 5.1 22 isoleucine HILIC 5.7 16 6.2 22 phenylalanine HILIC 4 16 4.6 22 tyrosine HILIC 5 16 5.4 22 tryptophan HILIC 3.7 16 5.8 22 proline HILIC 4.6 16 5.4 22 cis/trans-hydroxyproline HILIC 4.2 16 5.6 22 ornithine HILIC 13.3 16 12.0 22 citrulline HILIC 5.3 16 6.7 22 taurine HILIC 3.6 16 3.5 22 5-hydroxytryptophan HILIC 12.2 15 20.1 21 5-HIAA HILIC 6.9 16 7.2 22 serotonin HILIC 7.5 16 8.9 22 GABA HILIC 10.4 16 10.5 22 acetylglycine HILIC 11.2 15 13.1 22 dimethylglycine HILIC 2.8 16 5.9 22 argininosuccinate HILIC 14.6 16 11.9 22 ADMA HILIC 3.7 16 2.7 22 SDMA HILIC 4 16 6.0 22 NMMA HILIC 5.1 16 9.8 22 allantoin HILIC 3.4 16 4.9 22 aminoisobutyric acid HILIC 3.3 16 4.6 22 anthranilic acid HILIC 5.3 16 6.6 22 kynurenic acid HILIC 7.1 16 5.3 22 1-methylhistamine HILIC 4.2 16 4.4 22 5-adenosylhomocysteine HILIC 10 15 8.4 22 carnosine HILIC 6.4 16 8.2 22 histamine HILIC 14 16 14.3 22 3-hydroxyanthranilic acid HILIC 12.2 16 20.0 22 N-carbamoyl-beta-alanine HILIC 5.6 16 5.8 22 thiamine HILIC 4.5 16 11.2 22 niacinamide HILIC 7.1 16 7.5 22 betaine HILIC 2.1 16 2.6 22 choline HILIC 2.1 16 3.4 22 alpha-glycerophosphocholine HILIC 4.5 16 7.2 22 acetylcholine HILIC 3.1 16 5.4 22 creatine HILIC 2.4 16 3.3 22 creatinine HILIC 1.3 16 1.6 22 trimethylamine-N-oxide HILIC 2 16 2.1 22 Framingham Heart Study Offspring exam 6 - Urine panel Fox/Broad Intra- and Inter-assay CVs 2/4/2013 glycerol HILIC 14.9 16 11.6 22 glucose HILIC 7.2 16 11.2 21 adenosine HILIC 5 16 8.1 22 cytosine HILIC 4.8 16 13.1 22 thymidine HILIC 6.8 16 7.4 22 xanthosine HILIC 5.8 16 4.2 22 2-deoxyadenosine HILIC 9.4 16 6.2 22 2-deoxycytidine HILIC 13 16 13.8 22 cotinine HILIC 23.1 16 26.4 22 mevalonic acid HILIC 5 16 6.2 22 pipecolic acid HILIC 10.6 16 11.2 22 5-aminolevulinic acid HILIC 9.3 16 12.5 22 pyroglutamic acid HILIC 5 16 4.9 22 1-methylnicotinamide HILIC 2.6 16 4.4 22 butyrobetaine HILIC 2.4 16 4.2 22 putrescine HILIC 17.3 12 24.6 17 methionine sulfoxide HILIC 4.4 16 3.2 22 sarcosine HILIC 13.9 16 15.7 22 beta-alanine HILIC 5.9 16 7.9 22 anserine HILIC 6.9 16 6.9 22 carnitine HILIC 2.5 16 3.9 22 acetylcarnitine HILIC 3.5 16 3.3 22 propionylcarnitine HILIC 2.4 16 3.2 22 malonylcarnitine HILIC 3 16 5.0 22 butyrylcarnitine/isobutyrylcarnitine HILIC 3.3 16 4.7 22 valerylcarnitine/isovalerylcarnitine/2-methylbutyroylcarnitine HILIC 4.6 16 7.6 22 heptanoylcarnitine HILIC 2.3 16 6.1 22 octanoylcarnitine HILIC 4.3 16 6.2 22 nonanoylcarnitine HILIC 5.7 16 7.5 22 decanoylcarnitine HILIC 11.6 16 14.0 22 lauroylcarnitine HILIC 11.7 16 17.7 22.
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