Effect of Frozen Storage on the Chemical Composition of Sand Smelt (Atherina Hepsetus) Fish Burger and Finger El-Lahamy AA1*, Khalil KI2, El-Sherif SA1, Mahmud AA2
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13(1): 007-013 (2019) Journal of FisheriesSciences.com E-ISSN 1307-234X © 2019 www.fisheriessciences.com Research Article Effect of Frozen Storage on the Chemical Composition of Sand Smelt (Atherina hepsetus) Fish Burger and Finger El-Lahamy AA1*, Khalil KI2, El-Sherif SA1, Mahmud AA2 1National Institute of Oceanography and Fisheries, Fish Processing Technology Laboratory, Cairo, Egypt 2Food Science and Technology Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt Received: 21.01.2019 / Accepted: 01.03.2019 / Published online: 08.03.2019 Abstract: Sand smelt fish (Atherina hepsetus) one of the low economic fish and unacceptable from consumers in Qarun lake, Egypt. Sand smelt fish burger and finger made from sand smelt fish with soybean flour (SF) and minced boiled potatoes (MBP) to improve the economic value for fish. Changes in proximate composition of sand smelt fish products during frozen storage were determined. Chemical analysis indicated that moisture, protein and fat contents of burger and fingers samples gradually decreased during frozen storage period while ash and carbohydrate contents increased during frozen storage. No considerable changes in cooking loss could be observed in the early stages of frozen storage. Meanwhile, as the storage period prolonged, cooking loss values increased. The filling materials SF and MBP used in the production of fish products showed an observed effect in maintaining the chemical composition of the products for 90 days of frozen storage. It can be concluded that sand smelt fish products opening an opportunity for this good nutritious protein source to be used as a daily food. Keywords: Fish products; Soybean flour; Frozen storage; Bissaria; Cooking loss *Correspondence to: El-Lahamy, National Institute of Oceanography and Fisheries, Fish Processing Technology Laboratory, Cairo, Egypt, Tel: +201100425093; E-mail: [email protected] 7 Journal of FisheriesSciences.com El-Lahamy et al., 13(1): 007-013 (2019) Journal abbreviation: J FisheriesSciences.com Introduction Research Center (ARC) at Giza Governorate, Egypt. MBP was prepared by boiling potatoes for 15 min, peeled and minced. Fish is one of the most important sources of animal protein available in the tropics and has been widely accepted as a source Ingredients Sunflower oil, wheat flour, starch, sugar, salt, of high quality protein and other elements for the maintenance of garlic, onion, spices, and other additives were obtained from the healthy body (Gomma, 2005). Fish contain high quality protein, local market at Fayoum Governorate. low in saturated fatty acids and contain high content of unsaturated Preparation of fish fatty acids (Venugopal & Shahidi, 1995). There is a large quantity of very small fish landed as by-catch which do not find a ready Fish was gutted, beheaded and washed gently with tap water. market as fresh fish. Sand smelt (Atherina boyeri) lives in the The edible portion of sand smelt using an electric meat mincer Marmara Sea, Aral and Caspian Sea Black Sea and Aegean Sea (Braun plus 1300). The minced fish was divided into two portions, (Altun, 1999). The numbers of sand smelt fish (Bissaria) has kept to process fish products of burger and finger. increased in the Lake of Qarun In the recent years. Sand smelt Fish burger formulation and processing fish is characterized by the high edible portion and small size but Table 1 indicates the recipe of sand smelt burger as mentioned is unacceptable for consumer. Bissaria is an uneconomical fish by (Chandrasckhar and Mohite 1978) and sand smelt finger by in Egypt. In recent years, the increases civilizations have led to (Çakli et al., 2005 and Tokur et al., 2006). The experimental consumer's preference to ready-to-eat foods (Cakli et al., 2005). formulated burger and finger samples were made by replacing Fish burgers and fingers are one of the secondary minced fish based the minced fish with the filling material at the desired level as products and are popularly distributed frozen for longer shelf life illustrated in Table 1. All the formulations were needed by hand (Jamilah, 2004). There are few published studies on the effect of until homogenous dough was obtained. Portions of 50g were frozen storage on the chemical composition sand smelt fish burger shaped (8.5 cm diameter and 1.0 cm thickness) by manually and finger. Freezing is the process that lowers the temperature to operated forming machine (NOAW- Affetacrane, Italy). The below the freezing point. These temperatures allow most of the dough fish finger was shaped into fingers and frozen at -18C for water to turn into ice. The freezing point depends on the substances 2 h before battering. The frozen fish fingers were rapidly coated dissolved in the fluid of the tissue. Fish contain 75–80% water. with batter solution (3 parts of water plus 2 parts contained 94% The freezing process concerns the removal of latent heat during maize flour, 2% skim milk, 2% egg yolk and 2% salt) and then the phase transition of water from liquid to solid and removal of they were rubbed with ground crumb. Burger and finger samples sensible heat, depending on the reduction of temperature (Gokoglu were packaged in polyethylene bags and stored at -18°C until and Yerlikaya, 2015). Frozen storage has been widely employed to required for analysis. retain fish properties before it is consumed or employed in other Storage studies technological processes. Fish is usually processed in different techniques such as boiling, smoking, roasting, frying and grilling. The formulated samples along with the control samples of These processing and cooking techniques improved the hygienic burgers and fingers were packed in polyethylene bags and stored quality by inactivation of pathogenic microorganisms and in deep freezer at –18°C for 90 days. Samples of burger and finger enhanced the digestibility and bio-availability of nutrient in the were withdrawn periodically at intervals of 15 days for analysis. digestive tract (Kocatepe et al., 2011). Analytical methods Therefore, the overall aims of this study was to investigate Proximate chemical composition 1- Determine the changes in proximate composition of sand Moisture, crude protein, crude fat and ash contents were smelt frozen fish products stored at -18ºC for a 3 month period. determined according to AOAC (2012). Total carbohydrates were 2- Determine the changes in cooking loss of sand smelt fish Table 1: Recipes of sand smelt fish burger and fingers. burger and finger during frozen storage at -18 ̊ C, for 90 days. Fish burger Fish fingers Materials and Methods Ingredients % Ingredients % Fish mince 75 Fish mince 93.5 Fish Vegetable oil 9 Salt 1.5 About 25 kg of fresh sand smelt fish (Atherina hepsstia) was Starch 8 Sugar 1 obtained from Qarun Lake, Fayoum Governorate, Egypt during Salt 2.3 Wheat flour 3 April, 2017. Fresh fish were transported in ice-box to the laboratory Sodium bicarbonate 0.4 Cumin 0.24 of Fish processing and technology Research Station (at Fayoum), Onion 2.5 Onion 0.24 National Institute of Oceanography and Fisheries (NIOF). Garlic 0.5 Garlic powder 0.24 Polyphosphate 0.3 Black pepper 0.24 Filling materials *Spices mixture 2 Thyme 0.04 Filling materials used in the processing of fish products were *Spices mixture composed of 32% black pepper, 22.5% coriander, Soybean flour (SF) and Minced boiled potatoes (MBP). SF was 15% cumin, 10% cardamom, 9% red pepper, 7.5% cubeb and 4% obtained from Food Technology Research Institute, Agriculture clove. 8 Journal of FisheriesSciences.com El-Lahamy et al., 13(1): 007-013 (2019) Journal abbreviation: J FisheriesSciences.com calculated by difference method using the following equation: under the same conditions. Data presented in Table 3 indicated Total carbohydrates =100% - (% protein + % fat + % ash + % that moisture contents of control, 20% SF and 15% MBP finger moisture) (Merrill and Watt, 1973). sample gradually decreased from 64.77, 63.34 and 65.12% at day zero storage to 59.10, 58.15 and 61.82%, respectively. The Cooking loss total losses in moisture contents of sand smelt burger at the end Cooking loss and was determined and calculated as described of storage period were 8.42, 7.13 and 7.5% in control, 15% SF by Roland et al., (1981) using the following equations: and 15% MBP samples, respectively. Similarly moisture losses (Weight before cooking - weight after cooking) of control, 20% SF and 15% MBP samples were 10.22, 6.69 Cooking loss (%) = ×100 (weight before cooking) 5.10% respectively. This observation indicated that incorporation of filling materials in the processing of sand smelt fish products Sand smelt fish samples cooked by frying method improved the water binding capacity in these products during Statistical analysis: Data obtained were analyzed statistically frozen storage. The improving effect of filling materials was using the least significant difference test (LSD) at (p ≤ 0.05) and more pronounce in fish fingers particularly with minced boiled Standard Error (Mean ± SE) which calculated using SPSS 16.0 for potatoes. Similar findings were reported by Baaiu (1974), El- windows (SPSS Inc., Chicago, USA). Akeel (1983), Abo-zied (1995), Gomma (2005), Ibrahim and El- Sherif (2008) and Gandotra et al. (2012). Also these results agreed Results and Discussion with studies made on fish products processed from other types of fish. Orban et al. (2002). In sea bass (Dicentrarchus labrax) Chemical analysis was carried in the uncooked burger and fillets, moisture content decreased during frozen storage (Benjakul fingers samples to determine changes occurred in moisture, et al. 2005). This decrease in moisture content was attributed to protein, fat, ash and carbohydrates contents during frozen storage the sublimation of ice in frozen storage and the loss of drip during period. thawing process. Meanwhile, in contrary to these findings, in a Moisture study made on crab, Zamir et al. (1998) found an increasing trend in moisture content during storage and attributed this increase to Changes in moisture content of raw (uncooked) fish products the loss of water holding capacity of tissue.