Supplementary Table 3: Calcineurin- and Aging-Sensitive Genes

Supplementary Table 3: Calcineurin up/ Aging up (pages 1 -2)- genes classified as Ad-aCaN up in the present study (see Supplementary Table 1), as well as reported as significantly increased in our prior aging study (Blalock et al., 2003, see supplementary tables 3, 4 and 5). Calcineurin Down/ Aging Down (page 2), Calcineurin Up/ Aging Down (pages 2-3),and Calcineurin Down/Aging Up (page 3)as above except for direction of change. - Columns: Probe Set- Affymetrix probe set identifier for RG-U34A microarray, Symbol and Title- annotated information for above probe set (annotation downloaded June, 2004), Calcineurin ANOVA- p-value for 1-way Analysis of Variance.

Calcineurin Up/Aging Up Probe set Symbol Title CaN ANOVA rc_AI170268_at B2m beta-2 microglobulin 0.00000 rc_AI102299_s_at Bid3 BH3 interacting domain 3 0.00721 X52477_at C3 complement component 3 0.00000 X58294_at Ca2 carbonic anhydrase 2 0.00004 X76489cds_g_at Cd9 CD9 antigen 0.00000 L07736_at Cpt1a carnitine palmitoyltransferase 1, liver 0.00001 M55534mRNA_s_at Cryab crystallin, alpha B 0.00000 X60351cds_s_at Cryab crystallin, alpha B 0.00000 rc_AI234146_at Csrp1 cysteine and glycine-rich protein 1 0.00000 rc_AI176595_s_at Ctsl cathepsin L 0.00001 D00569_at Decr1 2,4-dienoyl CoA reductase 1, mitochondrial 0.00000 rc_AA924925_at Dri42 ER transmembrane protein Dri 42 0.00000 rc_AA848831_at Edg2 endothelial differentiation, lysophosphatidic acid GPCR, 2 0.00000 X14323cds_g_at Fcgrt Fc receptor, IgG, alpha chain transporter 0.00000 L00191cds#1_s_at Fn1 fibronectin 1 0.00013 rc_AI231807_at Ftl1 ferritin light chain 1 0.00000 rc_AI231807_g_at Ftl1 ferritin light chain 1 0.00000 rc_AA799645_at Fxyd1 FXYD domain-containing ion transport regulator 1 0.00031 rc_AA799645_g_at Fxyd1 FXYD domain-containing ion transport regulator 1 0.00000 U92081mRNA_s_at Gp38 glycoprotein 38 0.00399 X62322_g_at Grn granulin 0.00000 X78848cds_f_at Gsta1 glutathione S-transferase, alpha 1 0.00000 rc_AI012589_s_at Gstp2 glutathione S-transferase, pi 2 0.00001 X02904cds_s_at Gstp2 glutathione S-transferase, pi 2 0.00007 AF008554_at IAG2 implantation-associated protein 0.00007 rc_AI231213_g_at Kai1 kangai 1 0.00000 D90211_s_at Lamp2 lysosomal membrane glycoprotein 2 0.00004 M32016_at Lamp2 lysosomal membrane glycoprotein 2 0.00012 X54096_at Lcat lecithin cholesterol acyltransferase 0.00000 rc_AI237535_s_at Litaf LPS-induced TNF-alpha factor 0.00009 M23601_at Maob monoamine oxidase B 0.00066 J03752_at Mgst1 microsomal glutathione S-transferase 1 0.00002 U10357_g_at Pdk2 pyruvate dehydrogenase kinase 2 0.00002 AB000778_s_at Pld1 phospholipase D1 0.00000 S55427_s_at Pmp22 peripheral myelin protein 22 0.00000 Y17295cds_s_at Prdx6 peroxiredoxin 6 0.00000 M19936_at Psap prosaposin 0.02210 L40362_f_at RT1-Aw2 RT1 class Ib, locus Aw2 0.00000 M24026_f_at RT1-Aw2 RT1 class Ib, locus Aw2 0.00000 M11071_f_at RT1-Aw2 RT1 class Ib, locus Aw2 0.00037 M31018_f_at RT1-Aw2 RT1 class Ib, locus Aw2 0.00000 M24324_f_at RT1-Aw2 RT1 class Ib, locus Aw2 0.00001 X06916_at S100a4 S100 calcium-binding protein A4 0.00000 S53527mRNA_s_at S100b S100 protein, beta polypeptide 0.00000 S61868_g_at Sdc4 syndecan 4 0.02260 U37138_at Sts steroid sulfatase 0.00108 D38380_at Tf Transferrin 0.00000 D38380_g_at Tf Transferrin 0.00000 X62952_at Vim vimentin 0.00000

Page 1 Supplementary Table 3: Calcineurin- and Aging-Sensitive Genes

Probe set Symbol Title CaN ANOVA Calcineurin Down/Aging Down rc_AA942685_at Cdo1 cytosolic cysteine dioxygenase 1 0.00705 U66470_at Cgref1 cell growth regulator with EF hand domain 1 0.00002 rc_AA892506_at Coro1a coronin, actin binding protein 1A 0.00001 D89340_at Dpp3 dipeptidylpeptidase III 0.03376 AF023087_s_at Egr1 early growth response 1 0.00001 U78102_at Egr2 early growth response 2 0.00014 rc_AI230914_at Fntb farnesyltransferase beta subunit 0.00001 L21192_at Gap43 growth associated protein 43 0.01584 rc_AA799779_g_at Gnpat acyl-CoA:dihydroxyacetonephosphate acyltransferase 0.00012 D37951UTR#1_at Hivep2 human immunodeficiency virus type 1 enhancer-binding protein 2 0.00000 X54793_at Hsp60 heat shock protein 60 (liver) 0.02154 M24852_at Pcp4 Purkinje cell protein 4 0.00029 AB020504_at Pmf31 PMF32 protein 0.04777 U06099_at Prdx2 peroxiredoxin 2 0.02349 AA799389_at Rab3b Rab3B protein 0.00578 X78605_at Rab4b ras-related GTP-binding protein 4b 0.00186 L46873_s_at Slc15a1 solute carrier family 15 (oligopeptide transporter), member 1 0.00928 K02248cds_s_at Sst somatostatin 0.00000 M93273_at Sstr2 somatostatin receptor 2 0.00001 L42855_at TCEB2 transcription elongation factor B (SIII) polypeptide 2 (18kD, elongin B) 0.01315 M31174_at Thra thyroid hormone receptor alpha 0.03833 D10699_g_at Uchl1 ubiquitin carboxy-terminal hydrolase L1 0.00651 Calcineurin Up/Aging Down U89905_at Amacr alpha-methylacyl-CoA racemase 0.04540 AF087037_at Btg3 B-cell translocation gene 3 0.00118 M27207mRNA_s_at Col1a1 collagen, type 1, alpha 1 0.00195 U75405UTR#1_f_at Col1a1 collagen, type 1, alpha 1 0.01215 M21354_s_at Col3a1 collagen, type III, alpha 1 0.00080 U90610_g_at Cxcr4 Chemokine receptor (LCR1) 0.00000 J02773_at Fabp3 fatty acid binding protein 3 0.02837 rc_AI237731_s_at Lpl lipoprotein lipase 0.00766 X84039_at Lum lumican 0.00035 X76985_at Lxn latexin 0.00000 M83745_at Pcsk2 proprotein convertase subtilisin/kexin type 2 0.03212 U28938_at Ptpro protein tyrosine phosphatase, receptor type, O 0.00111 X98399cds_s_at Slc14a1 solute carrier family 14, member 1 0.00695 M15191_s_at Tac1 tachykinin 1 0.00000 rc_AI136891_at Zfp36l1 zinc finger protein 36, C3H type-like 1 0.00015 Calcineurin Down/Aging Up M77245_at Ap1b1 adaptor protein complex AP-1, beta 1 subunit 0.00022 U67137_at Dlgap1 guanylate kinase associated protein 0.00621 rc_AA892775_at Lyz lysozyme 0.00007

Page 2