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Supplement of Screening of Cloud Microorganisms Isolated at the Puy De Dôme (France) Station for the Production of Biosurfactants

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Supplement of Screening of Cloud Microorganisms Isolated at the Puy De Dôme (France) Station for the Production of Biosurfactants Supplement of Atmos. Chem. Phys., 16, 12347–12358, 2016 http://www.atmos-chem-phys.net/16/12347/2016/ doi:10.5194/acp-16-12347-2016-supplement © Author(s) 2016. CC Attribution 3.0 License. Supplement of Screening of cloud microorganisms isolated at the Puy de Dôme (France) station for the production of biosurfactants Pascal Renard et al. Correspondence to: Anne-Marie Delort ([email protected]) The copyright of individual parts of the supplement might differ from the CC-BY 3.0 licence. Table S1. Strains are isolated during 39 cloud events (from 2004 to 2014) gathered in four categories according to the physicochemical characteristics of the cloud waters (Blue: marine, purple: highly marine, green: continental and black: polluted) as described by Deguillaume et al. (2014). Cloud Nb of Ions (µM) Composition Date pH 2- - - + + 2+ + 2+ Event strains SO 4 NO 3 Cl Acetate Formate Oxalate Succinate Malonate Na NH 4 Mg K Ca 21 Marine 2 2004-01 5.6 NA NA NA NA NA NA NA NA NA NA NA NA NA 23 Polluted 2 2004-02 3.1 NA NA NA NA NA NA NA NA NA NA NA NA NA 29 Marine 1 2004-07 5.5 NA NA NA NA NA NA NA NA NA NA NA NA NA 30 Marine 3 2004-09 7.6 3.8 6.5 12.0 6.0 6.0 0.5 0.1 0.16 19.4 54.9 4.9 4.1 12.0 32 Continental 1 2004-12 5.5 72.1 95.8 31.5 0.0 0.3 0.3 0.1 0.17 74.4 132.4 7.6 9.0 73.7 42 Continental 25 2007-12 4.7 39.7 198.4 20.2 10.2 5.8 2.9 0.6 0.58 19.1 148.2 3.5 11.9 58.0 43 Highly marine 25 2008-01 5.9 9.4 21.4 81.4 11.4 6.7 1.2 0.2 0.28 315.7 35.9 11.8 13.7 26.0 44 Continental 14 2008-02 5.2 24.6 65.9 17.2 26.8 18.0 1.3 0.5 0.39 15.2 148.5 1.8 5.8 22.3 45 Continental 22 2008-04 4.6 44.7 65.5 31.7 17.7 17.9 2.8 0.9 0.89 33.3 122.5 4.9 9.7 53.6 46 Continental 2 2008-10 5.0 13.2 102.4 28.7 2.7 14.2 1.3 0.4 0.52 10.4 72.7 6.9 5.3 77.7 47 Marine 14 2008-11 5.4 6.5 33.2 15.7 5.3 8.0 0.8 0.2 0.33 8.2 13.7 1.4 6.3 14.4 49 Marine 6 2009-01 6.5 14.7 20.0 113.9 4.8 8.9 1.2 0.3 0.44 70.9 58.2 29.2 12.5 14.5 50 Marine 7 2009-02 4.9 7.1 23.3 72.5 22.2 13.4 0.8 0.2 0.56 NA NA NA NA NA 53 Polluted 8 2009-03 4.0 73.8 516.5 193.8 41.2 13.7 3.5 1.2 0.62 171.9 363.2 13.5 71.6 52.3 54 Marine 62 2009-11 5.2 2.3 13.3 30.5 4.4 15.0 1.7 0.1 0.34 37.0 6.6 12.1 20.5 0.0 55 Marine 11 2009-11 5.8 9.3 34.8 97.5 6.7 10.1 3.3 0.3 0.36 95.1 31.1 12.6 17.2 0.0 60 Highly marine 32 2010-03 5.5 39.0 9.7 231.5 3.5 4.3 1.2 0.0 0.00 114.1 28.6 12.9 12.8 2.7 61 Marine 2 2010-05 6.2 3.1 6.0 11.6 6.0 6.4 0.1 0.0 0.00 11.8 15.4 0.2 6.2 4.2 62 Marine 1 2010-06 6.1 3.5 4.5 2.3 3.4 6.2 1.4 0.0 0.00 1.8 6.0 0.0 0.0 0.0 66 Marine 1 2010-09 5.7 4.0 17.8 1.5 7.1 8.6 2.6 0.2 0.00 2.3 32.4 0.2 0.7 0.5 71 Marine 2 2011-03 5.9 3.5 6.6 6.2 7.1 4.2 2.2 0.3 0.39 6.9 42.5 0.1 3.6 5.1 72 Marine 5 2011-03 7.0 12.7 26.0 26.4 16.6 12.7 1.8 0.5 0.53 40.2 75.1 0.4 12.5 15.2 75 Marine 9 2011-06 5.2 NA NA NA NA NA NA NA NA NA NA NA NA NA 76 Continental 4 2011-06 5.9 52.2 126.0 16.9 NA NA NA NA NA NA NA NA NA NA 77 Continental 11 2011-07 6.0 19.6 47.9 12.8 58.3 109.6 12.3 NA NA 124.8 408.5 26.8 22.8 148 78 Marine 8 2011-07 5.5 7.1 10.4 24.1 3.3 11.3 3.1 NA NA 22.7 32.7 12.3 9.2 7.1 79 Marine 5 2011-11 4.6 1.3 5.0 0.3 4.3 5.6 1.0 NA NA 0.6 52.7 0.8 1.6 3.2 80 Marine 2 2012-01 4.9 1.8 2.2 13.4 7.4 5.3 1.1 NA NA 80.6 39.3 12.0 6.1 5.2 81 Marine 7 2012-01 5.8 4.8 11.2 23.4 15.3 15.6 6.7 NA NA 145.7 188.6 20.3 6.2 9.3 82 Marine 2 2012-03 5.3 1.6 2.0 0.2 4.2 6.2 3.0 NA NA 1.0 75.4 1.0 0.1 6.1 83 Continental 10 2012-04 5.6 10.7 49.9 12.2 0.0 39.0 6.5 NA NA 93.5 531.1 14.3 10.7 34.6 84 Marine 21 2012-04 5.5 1.6 1.9 6.0 3.9 7.7 1.2 NA NA 36.0 38.1 6.0 1.4 5.2 85 Marine 17 2012-06 5.5 1.2 3.0 0.9 0.0 18.2 3.3 NA NA 6.0 77.9 2.2 4.0 4.3 86 Marine 42 2012-09 5.9 0.5 1.0 1.0 3.2 3.2 1.0 NA NA 8.8 16.8 1.4 5.5 4.5 87 Marine 28 2012-10 6.2 5.3 11.0 26.7 4.0 13.6 0.7 NA NA 21.2 39.3 8.3 7.6 2.3 88 Polluted 2 2012-11 4.6 111.1 346.5 47.6 18.5 13.1 6.5 NA NA 17.6 59.3 15.8 53.6 6.6 89 Marine 31 2013-01 5.2 32.9 29.5 109.9 19.9 15.6 2.7 NA NA 77.5 81.2 21.5 4.8 23.5 91 Marine 7 2013-05 5.5 28.5 9.3 16.3 21.8 9.8 3.3 NA NA 13.3 57.4 6.2 8.0 22.2 97 Marine 26 2014-02 5.8 NA NA NA NA NA NA NA NA NA NA NA NA NA 2 Table S2. 480 strains tested for biosurfactant production. Strains are isolated during 39 cloud events (from 2004 to 2014) gathered in four categories according to the physicochemical characteristics of the cloud waters (Blue: Marine, purple: Highly marine, green: Continental and black: Polluted, see table S1) as described by Deguillaume et al. (2014). Strains are differentiated into four main categories, according to the measured surface tension (red: σ ≤ 30, orange: 30 < σ ≤ 45, yellow: 45 < σ ≤ 55 and green: σ > 55 mN m -1, see details in text). Phylum colors correspond to those of Figure 1. All surface tension measurements are performed using the pendant drop method with an OCA 15 Pro tensiometer (Data Physics, Germany). A.N, accession number in GenBank. Cloud σ Composition Strain Phylum (class) Species AN Events (mN m -1) 76 Continental 49b04 ϒ-Proteobacteria Pseudomonas sp. KR922066 24.4 77 Continental 50b03 ϒ-Proteobacteria Pseudomonas sp. KR922069 25.6 77 Continental 50b08 ϒ-Proteobacteria Pseudomonas sp. KR922074 25.6 77 Continental 50b04 ϒ-Proteobacteria Pseudomonas sp. KR922070 25.9 77 Continental 50b02 ϒ-Proteobacteria Pseudomonas sp. KR922068 26.1 45 Continental 26b18 Basidiomycota Udeniomyces sp. JF706566 33 44 Continental 25b01 Basidiomycota Bullera globispora HQ260318 36 77 Continental 50b07 ϒ-Proteobacteria Pseudomonas syringae KR922073 36 42 Continental 23b16 Actinobacteria Leifsonia sp. HQ256777 39 77 Continental 50b09 ϒ-Proteobacteria Pseudomonas syringae KR922075 39 44 Continental 25b04 Basidiomycota Udeniomyces sp. HQ256877 39 76 Continental 49b03 Unidentified yeast unidentified 42 44 Continental 25b05 ϒ-Proteobacteria Pseudomonas sp. HQ256806 43 45 Continental 26b25 β-Proteobacteria Variovorax sp. HQ256810 44 44 Continental 25b07 ϒ-Proteobacteria Erwinia billingiae HQ256807 46 44 Continental 25b11 ϒ-Proteobacteria Erwinia billingiae HQ256802 46 44 Continental 25b13 ϒ-Proteobacteria Erwinia billingiae HQ256804 46 77 Continental 50b05 ϒ-Proteobacteria Pseudomonas sp.
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