Plant Pathology Bulletin 18: 247-262, 2009 Pratylenchus penetrans P. loosi (Nematoda: Pratylenchidae) 1, 6 2 3 4 5 1. 2. 3. 4. 5. 6.
[email protected] +886-4-2330-2803 99 3 31 . 2009. Pratylenchus penetrans P. loosi (Nematoda: Pratylenchidae) . 18: 247-262 2007 10 9 (Pratylenchus spp.) 5 P. penetrans (Cobb, 1917) Chitwood & Oteifa, 1952 4 P. loosi Loof, 1960 SEM (morphometrics) 5.8S ITS-1 ITS-2 18S 28S DNA (ribosomal DNA, rDNA) P. penetrans P. loosi rDNA GenBank 916 bp 1238 bp 80.9 % DNA Pratylenchus penetrans Pratylenchus loosi (1964) (23) Toung (1962, 1963) (39,40) Hung (1966) (26) Wu (root-lesion nematode, Pratylenchus (2002) (44) Filipjev, 1936) 19 (cortex) (3, 30) (35) (root-knot nematode, Meloidogyne spp.) (scanning electron microscope, SEM) P. P. coffeae P. brachyurus (26) P. coffeae (25, 44) P. loosi (43, 44) P. penetrans penetrans P. loosi P. zeae Chen (26, 44) P. pratensis (23, 39, 40) P. zeae (11) 6 (2009) (11) P. pratensis P. brachyurus Hu Chu 248 18 4 2009 Pratylenchus spp. Secernentea von Linstow, 1905 (class) Tylenchida Thorne, 1949 (order) Hoplolaimina Chizhov & 1 Berezina, 1988 (suborder) Hoplolaimoidea Filipjev, 1934 (Paramonov, 1967) (superfamily) Pratylenchidae Thorne, 1949 (Siddiqi, 1963) 4 100 (family) (37) Pratylenchus (flat) (stylet) (basal knot) (intestine) 20-80 70-80 % (3, 37) (species) 30-50 (genomic DNA) 2% (formaldehyde de Man's formula solution) SEM (morphometrics) (valid species) 49 (17) 55 (2) 63 (19) (32) (entomopathogenic) (21, 22, 33) (5, 7, 9, 12, 15, 16, 20, 27, 28, 34, 38) (ribosomal DNA, rDNA) 3-5 200 2007 10 400 (excretory pore) (hemizonid) (median bulb) 5 (populations) 1000 4 (stylet) (spicule) SEM (orifice of dorsally esophageal gland) rDNA (phasmid) Chen (4) P.