DOCSLIB.ORG

Genetic Heterogeneity Reveals On-Going Speciation And

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Genetic Heterogeneity Reveals On-Going Speciation And View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by University of Debrecen Electronic Archive Genetic Heterogeneity Reveals On-Going Speciation and Cryptic Taxonomic Diversity of Stream-Dwelling Gudgeons (Teleostei, Cyprinidae) in the Middle Danubian Hydrosystem (Hungary) Pe´ter Taka´cs1*,Pe´ter Bihari2, Tibor Ero˝s1, Andra´s Speczia´r1, Ildiko´ Sziva´k1,Pe´ter Bı´ro´ 1, Eszter Csoma3 1 Department of Hydrozoology, Balaton Limnological Institute, MTA Centre for Ecological Research, Tihany, Hungary, 2 Institute of Genetics, MTA Biological Research Centre, Szeged, Hungary, 3 Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary Abstract Although stream-dwelling gudgeons (Cyprinidae, genus: Gobio) are widespread in Central Europe, the taxonomy of this group and the distribution of its species are still unexplored in detail. The aims of our study are to ascertain taxonomic composition and distribution of the former Gobio gobio superspecies in the inner area of the Carpathian Basin. Since the presence of cryptic species is suspected in this area, we examined the taxonomic and phylogenetic relationships of Central European Gobio taxa by sequencing the mitochondrial DNA control region (mtCR). Additionally, we characterized the genetic structure of 27 stream-dwelling gudgeon populations of this area by Amplified Fragment Length Polymorphism (AFLP). Results of mtCR analysis proved the presence of three species already known as G. obtusirostris (dominant in NW- Hungary), G. gobio (sporadic) and G. carpathicus (sporadic). Additionally, the analysis revealed the existence of one doubtful taxon, G. sp1 (dominant in NE-Hungary), and a new isolated haplogroup (dominant in SW-Hungary). Although Network analysis showed significant detachment among haplogroups, their genetic distances were quite small. Therefore Bayesian phylogenetic analysis showed weak nodal support for the branching pattern both for newly described haplotypes, and for the already accepted species. AFLP data showed distinct population structure and a clear pattern of isolation was revealed by distance of stocks. At the same time, level of separation was not affected by the altitudinal position of sites. Moreover we found three major clusters of populations which were separated according to hydrographic regions, and corresponded to the findings of mtCR analysis. Our results suggest the on-going speciation of gudgeons in the Carpathian Basin, however the separation of haplogroups seems to only be an intermediate phase. The discovered natural pattern seems to be only slightly influenced by anthropogenic impacts. Additionally our results put into question the suitability of the recently accepted within Gobio genus taxonomy. Citation: Taka´cs P, Bihari P, Ero˝s T, Speczia´r A, Sziva´k I, et al. (2014) Genetic Heterogeneity Reveals On-Going Speciation and Cryptic Taxonomic Diversity of Stream-Dwelling Gudgeons (Teleostei, Cyprinidae) in the Middle Danubian Hydrosystem (Hungary). PLoS ONE 9(5): e97278. doi:10.1371/journal.pone.0097278 Editor: Jesus E. Maldonado, Smithsonian Conservation Biology Institute, United States of America Received July 14, 2013; Accepted April 18, 2014; Published May 13, 2014 Copyright: ß 2014 Taka´cs et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The study was supported by the Hungarian Scientific Research Fund (OTKA-NKTH CNK 80140). ‘‘The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript’’. Competing Interests: The authors have declared that no competing interests exist. * E-mail: [email protected] Introduction species (i.e. on-going speciation) [10], [11], [12], [13], [14]. Such phenomena are more likely to occur in organisms with limited Freshwaters are exceedingly diverse ecosystems, but at the same dispersion ability and/or in organisms living in separated or time they are extremely sensitive to habitat degradation and narrowly connected habitats [15], [16], [17]. Many stream- pollution [1]. Accurately quantifying their taxonomic and dwelling fishes have specific environmental needs and therefore functional diversity is a fundamental requirement of conservation form discrete populations, not only between geographical areas biological, ecological, biogeographical, and macroevolutionary with separated catchment systems, but also between closely related research [2], [3], [4]. However, when species are not clearly sites of the same catchment [18], [19], [20], [21]. These isolated distinguishable by the conventional methods using ecological and fish populations may then genetically differentiate with time, morphological traits, have highly similar environmental needs and although they may still maintain their similar morphological reveal a high level of phenotypic plasticity, then estimates of appearance and ecological function [22]. However, this process is biodiversity and ecosystem functioning will be biased [5], [6], [7], not yet fully understood in seemingly well connected catchment [8]. systems. With the increasing use of molecular techniques, it has become The type species of the Gobioninae subfamily (Fam: Cyprinidae), evident that many species formerly believed to have widespread the common gudgeon Gobio gobio Linnaeus (1758), was known as geographical distribution can in fact be divided into numerous the most widely distributed lentic gudgeon species in West Eurasia more or less discrete entities -so called cryptic or sibling species by Mayr [9]- or represent genetic gradients between separating PLOS ONE | www.plosone.org 1 May 2014 | Volume 9 | Issue 5 | e97278 Cryptic Speciation of Stream-Dwelling Gobio [23]. However, the high between and within population morpho- 3714-2/2009, 14/1237/2/2010, 14/881/5/2011, 14/678-9/ logical variability of this taxon [24] resulted a long-standing debate 2012). Fish collected for this study were narcotized using clove regarding its taxonomical status [25], [26]. As a result, numerous oil. After fin tissue sampling, when they regained consciousness, forms/varietas/subspecies were described, nearly all from larger they were returned to the wild. Field studies did not involve fish catchment areas within its range (e.g. [23]). Recent genetic studies that were endangered (The IUCN Red List of Threatened Species have raised some of the former subspecies to species level and v. 2013.1; www.iucnredlist.org). several new species have been described as well [27], [28] [29], [30], [31], [32]. However, the taxonomic and genetic status of Study Area gudgeon populations in the Carpathian Basin is still unclear. Gobio All sampling sites were situated in the inner area of the gobio was considered a common species in the waters of the Carpathian Basin, which belongs to the drainage system of the Carpathian Basin for a long time [33], [34]. On the contrary, Danube River. Based on its topographic characteristics, the recent studies [31], [35] excluded the Carpathian Basin from the Hungarian part of Middle Danubian hydrosystem can be divided potential range of G. gobio and suggested the occurrence of the into two larger catchments and ten smaller sub-catchments Danube gudgeon, Gobio obtusirostris Valenciennes (1842) in the (Table 1, Figures 1 and 2). The hydrography of this area is western region of the basin, and the Carpathian gudgeon, Gobio biaxial. From the western region, all watercourses empty into the carpathicus Vladykov (1925) in the drainage system of River Tisza. Danube River. The eastern part of the country belongs to the Furthermore, Mendel et al. [31] indicated the presence of a drainage system of the Tisza River, which is the largest tributary of ‘‘species’’ (sic!: Gobio sp1) from the catchment of River Tisza, but the Danube (157 000 km2 catchment area). The structure of this the taxonomic position of this newly described taxon has not yet drainage system is dendritic, with the Tisza River forming the been firmly established. It is also important to note that the central axis. All the studied watercourses connected to the middle findings of Kottelat and Freyhof [35] are based exclusively on data section of the River Tisza [42], therefore this region was not from the literature. Moreover, although the study of Mendel et al. differentiated further. The hydrography of the Danubian system is [31] is the most comprehensive genetic study on the Middle more complicated. This system consists of three comparatively European Gobio species to date, it included only a very limited isolated subsystems: North, Middle and South Danubian regions number of samples from the Carpathian Basin and furthermore all (Table 1). North Danubian region is formed by the drainages of of those samples originated from the edges of this region. The River Ra´ba (Raab), River Ipoly (Ipel) and by the drainages of above mentioned Gobio taxa (i.e. G. gobio, G. obtusirostris, G. some direct inflowing streams. Middle Danubian catchment carpathicus and G. sp1) are morphologically very similar [35], [36] originally joined to the River Danube through a marshy area. and thus their distribution and ecology cannot be explored without Until the construction of the Sio´ canal at the end of the 19th molecular justification [24]. Furthermore, recent studies on G. century, there was only intermittent connection between
Load more

Recommended publications
  • Gobio Gobio (Linnaeus, 1758). Gobio AUTÓCTONA Catalán; Gobi
    Atlas y Libro Rojo de los Peces Continentales de España ESPECIE Gobio gobio (Linnaeus, 1758). Gobio AUTÓCTONA Catalán; Gobi. Vasco; Gobioa. Portugués; GÓbio. DESCRIPCIÓN Ciprínido de pequeño tamaño, que raramente supera los 15 cm de longitud. La forma de su cuer­ po refleja su condición de pez de fondo con una parte ventral más o menos plana, un dorso conve­ xo y la boca ínfera, con una barbilla a cada lado. El co­ lor del dorso es oscuro y en los flancos aparece una Clase: Actinopterygii línea de manchas redondeadas y azuladas sobre un Orden: Cypriniformes fondo más claro. La aleta dorsal y aleta caudal, fuerte­ Familia: Cyprinidae mente escotada, están irregularmente punteadas. Tiene Sinonimias: Cyprinus gobio Linnaeus, 1758. Leuciscus entre 37 y 41 escamas en la línea lateral. gobio (Linnaeus, 1758). Cobítis fundulus Wulff, 1765. Gobio fluviatilís Fleming, 1828. Gobio phoxinoides De BIOLOGÍA Y ECOLOGÍA la Pylaie, 1835. Gobio vulgaris Heckel, 1836. Gobio sa­ xatilis Koch, 1840. Gobio obtusirostris Valenciennes, Los adultos pueden encontrarse en fondos más o 1842. Gobio fluviatilis Cuvier, 1842. Bungia nigrescens menos blandos o cubiertos por sedimentos y también Keyserling, 1861. Gobio fluviatílís cynocephalus Dy­ en ambientes más lénticos, siempre que haya lugares bowski, 1869. Gobio fluviatilis lepidolaemus Kessler, cercanos donde existan las condiciones necesarias para 1872. Gobio gobio lepidolaemus Kessler, 1872. Gobio le­ su reproducción Se alimenta básicamente de macroin­ pidolaemus Kessler, 1872. Gobio lepidolaemus caucasi­ ca Kamensky, 1901. Gobio latus Ankin, 1905. Gobio go­ vertebrados bentónicos (larvas de insectos, crustáceos bio brevicirris Berg, 1914. Gobio gobio katopyga Berg, y moluscos). 1914. Gobio gobio longicirris Berg, 1914.
    [Show full text]
  • Teleostei: Cyprinidae), in the Upper Vistula Drainage (Southeast Poland)
    Arch. Pol. Fish. (2011) 19: 37-49 DOI 10.2478/v10086-011-0005-8 RESEARCH ARTICLE Contributions to the morphological variation of the common gudgeon, Gobio gobio complex (Teleostei: Cyprinidae), in the upper Vistula drainage (southeast Poland) Micha³ Nowak, Jan Mendel, Pawe³ Szczerbik, Artur Klaczak, Tomasz Miko³ajczyk, Konrad Ozga, Barbara Fa³owska, W³odzimierz Popek Received – 09 November 2010/Accepted – 10 February 2011. Published online: 30 March 2011; ©Inland Fisheries Institute in Olsztyn, Poland Abstract. Recent molecular research indicates that several Introduction distinct species have been confused under the name Gobio gobio (L.); thus, comparative investigations of numerous local populations are urgently needed. The present study presents Gobioninae are a subfamily of the family Cyprinidae and discusses detailed morphometric characteristics of 82 comprising small, bottom-dwelling fishes of practi- individuals from the G. gobio complex of nine tributaries of cally no commercial importance (Kottelat and the upper Vistula River (southeast Poland) within the context Freyhof 2007, Mendel et al. 2008a, 2008b, Nowak et of the known variability of this species. The specimens al. 2008a). This is why they have long been disre- analyzed generally resemble G. gobio s. stricto in a number of garded as a subject of ichthyological investigations in morphometric and meristic characters. The results are rather consistent with previous literature data on Polish populations Poland, as well as in Europe in general. Nevertheless, of the “common gudgeon”; however, as long as molecular they have been investigated extensively by taxono- analyses are not available, precise identification is impossible. mists. It was Bãnãrescu (1961) who divided the ge- nus Gobio into the three subgenera, namely Gobio Keywords: biometrics, Gobioninae, ichthyofauna, sensu stricto, Romanogobio, and Rheogobio.
    [Show full text]
  • Systematic List of the Romanian Vertebrate Fauna
    Travaux du Muséum National d’Histoire Naturelle © Décembre Vol. LIII pp. 377–411 «Grigore Antipa» 2010 DOI: 10.2478/v10191-010-0028-1 SYSTEMATIC LIST OF THE ROMANIAN VERTEBRATE FAUNA DUMITRU MURARIU Abstract. Compiling different bibliographical sources, a total of 732 taxa of specific and subspecific order remained. It is about the six large vertebrate classes of Romanian fauna. The first class (Cyclostomata) is represented by only four species, and Pisces (here considered super-class) – by 184 taxa. The rest of 544 taxa belong to Tetrapoda super-class which includes the other four vertebrate classes: Amphibia (20 taxa); Reptilia (31); Aves (382) and Mammalia (110 taxa). Résumé. Cette contribution à la systématique des vertébrés de Roumanie s’adresse à tous ceux qui sont intéressés par la zoologie en général et par la classification de ce groupe en spécial. Elle représente le début d’une thème de confrontation des opinions des spécialistes du domaine, ayant pour but final d’offrir aux élèves, aux étudiants, aux professeurs de biologie ainsi qu’à tous ceux intéressés, une synthèse actualisée de la classification des vertébrés de Roumanie. En compilant différentes sources bibliographiques, on a retenu un total de plus de 732 taxons d’ordre spécifique et sous-spécifique. Il s’agît des six grandes classes de vertébrés. La première classe (Cyclostomata) est représentée dans la faune de Roumanie par quatre espèces, tandis que Pisces (considérée ici au niveau de surclasse) l’est par 184 taxons. Le reste de 544 taxons font partie d’une autre surclasse (Tetrapoda) qui réunit les autres quatre classes de vertébrés: Amphibia (20 taxons); Reptilia (31); Aves (382) et Mammalia (110 taxons).
    [Show full text]
  • Increased Spatial Resolution of Sampling in the Carpathian Basin
    Takács et al. BMC Zoology (2021) 6:3 https://doi.org/10.1186/s40850-021-00069-7 BMC Zoology RESEARCH ARTICLE Open Access Increased spatial resolution of sampling in the Carpathian basin helps to understand the phylogeny of central European stream- dwelling gudgeons Péter Takács1*, Árpád Ferincz2, István Imecs3, Balázs Kovács2, András Attila Nagy4,5, Katalin Ihász2, Zoltán Vitál1,6 and Eszter Csoma7 Abstract Background: Phylogenetic studies of widespread European fish species often do not completely cover their entire distribution area, and some areas are often excluded from analyses than others. For example, Carpathian stocks are often omitted from these surveys or are under-represented in the samples. However, this area served as an extra- Mediterranean refugia for many species; therefore, it is assumed that fish stocks here may show special phylogenetic features. For this reason, increased spatial resolution of sampling, namely revealing genetic information from unexamined Carpathian areas within the range of doubtful taxa, may help us better understand their phylogenetic features. To test this hypothesis, a phylogenetic investigation using a partial mtCR sequence data was conducted on 56 stream-dwelling freshwater fish (Gobio spp.) individuals collected from 11 rivers of the data- deficient Southeastern Carpathian area. Moreover, we revieved the available phylogenetic data of Middle-Danubian stream-dwelling gudgeon lineages to delineate their distribution in the area. Results: Seven out of the nine detected haplotypes were newly described, suggesting the studied area hosts distinct and diverse Gobio stocks. Two valid species (G. obtusirostris, G. gobio), and a haplogroup with doubtful phylogenetic position” G. sp. 1" were detected in the area, showing a specific spatial distribution pattern.
    [Show full text]
  • Gudgeon (Gobio Gobio) ERSS
    Gudgeon (Gobio gobio) Ecological Risk Screening Summary U.S. Fish and Wildlife Service, April 2011 Revised, April 2018 Web Version, 4/30/2018 Photo: J. C. Schou, Biopix. Licensed under Creative Commons BY-NC. Available: http://eol.org/data_objects/19163802. (April 2018). 1 Native Range and Status in the United States Native Range From Froese and Pauly (2018): “Europe: Atlantic Ocean, North and Baltic Sea basins, from Loire drainage eastward, eastern Great Britain, Rhône and Volga drainages, upper Danube and middle and upper Dniestr and Dniepr drainages; in Finland, north to about 61°N. […] Eastern and southern limits unclear [Kottelat and Freyhof 2007]. Occurs as far east as Korea [Robins et al. 1991].” 1 From Freyhof (2011): “Austria; Belarus; Belgium; Czech Republic; Denmark; Estonia; Finland; France; Germany; Latvia; Liechtenstein; Lithuania; Luxembourg; Netherlands; Norway; Poland; Russian Federation; Slovakia; Sweden; Switzerland; Ukraine; United Kingdom” Status in the United States This species has not been reported as introduced or established in the United States. No documentation was found to suggest trade of this species occurs in the United States. Means of Introduction into the United States This species has not been reported as introduced or established in the United States. Remarks From Freyhof (2011): “Usually considered to be a morphologically variable species, with different morphologies reflecting adaptations to different habitats. Kottelat and Freyhoff's [sic; 2007] morphological and molecular data indicate that in
    [Show full text]
  • Distribution of Endangered Italian Gudgeon Romanogobio Benacensis (Cypriniformes, Cyprinidae, Gobioninae) with Remarks on Distinguishing Morphological Characters
    A peer-reviewed open-access journal ZooKeys 729: 103–127Distribution (2018) of endangered Italian gudgeon Romanogobio benacensis... 103 doi: 10.3897/zookeys.729.20615 RESEARCH ARTICLE http://zookeys.pensoft.net Launched to accelerate biodiversity research Distribution of endangered Italian gudgeon Romanogobio benacensis (Cypriniformes, Cyprinidae, Gobioninae) with remarks on distinguishing morphological characters Dušan Jelić1,2, Mišel Jelić3, Petar Žutinić3, Ivana Šimunović1, Primož Zupančič4, Alexander M. Naseka4,5 1 Croatian Institute for Biodiversity, Maksimirska cesta 129/5, HR-10000 Zagreb, Croatia 2 BIOTA j.d.o.o./ Ltd, Braće Radića 128A, HR-43290 Grubišno Polje, Croatia 3 University of Zagreb, Faculty of Science, De- partment of Biology, Rooseveltov trg 6, HR-10000 Zagreb, Croatia 4 Dinaric Research Institute, Dolsko 14, SI-1262 Dol pri Ljubljani, Slovenia 5 Faculty for Biology and Soil, Saint Petersburg State University, Univer- sitetskaya Emb. 7/9, Saint Petersburg 199034, Russia Corresponding author: Dušan Jelić ([email protected]) Academic editor: M. Bichuette | Received 26 August 2017 | Accepted 20 October 2017 | Published 16 January 2017 http://zoobank.org/F9BA4C98-F57C-4138-961D-900C329A6A16 Citation: Jelić D, Jelić M, Žutinić P, Šimunović I, Zupančič P, Naseka AM (2018) Distribution of endangered Italian gudgeon Romanogobio benacensis (Cypriniformes; Cyprinidae; Gobioninae) with remarks on distinguishing morphological characters. ZooKeys 729: 103–127. https://doi.org/10.3897/zookeys.729.20615 Abstract Distribution data on many freshwater fish species in Croatia are scarce and species identifications are dif- ficult, requiring further detailed studies. This paper presents a report of the Italian gudgeonRomanogobio benacensis from the Mirna River in the Istra Peninsula in Croatia, in the south-east from its previously known distribution range.
    [Show full text]
  • Researches on the Fish Fauna in Some Scis Natura 2000 from Romania Aurel Năstase, Vasile Oțel
    Researches on the fish fauna in some SCIs Natura 2000 from Romania Aurel Năstase, Vasile Oțel Danube Delta National Institute for Research and Development, Tulcea, Romania. Corresponding author: A. Năstase, [email protected] Abstract. In the autumn 2010 the fish fauna of water basins from 12 sites of community interest (SCI) Natura 2000 were investigated as follows: river Mureș (ROSCI0108 Lunca Mureșului Inferior), river Timiș (ROSCI0109 Lunca Timișului), rivers Târg, Argeșel and Râușor (ROSCI0381 Râul Târgului- Argeșel-Râușor), river Nera (ROSCI0031 Cheile Nerei – Beușnița), river Ialomița (ROSCI0290 Coridorul Ialomiței), river Little Danube (ROSCI0173 Pădurea Stârmina), river Suceava (ROSCI0379 Râul Suceava), Danube-Black Sea channel (ROSCI0398 Straja-Cumpăna), valleys Argel and Moldovița (ROSCI0328 Obcinele Bucovinei), river Siret (ROSCI0162 Lunca Siretului Inferior), Casimcea valley (ROSCI0215 Recifii Jurasici Cheia) and Danube (ROSCI0044 Corabia-Turnu Măgurele). The researches were focused on the fish species of community interest (Annex II of Habitat Directive), its position in the ichthyocoenose (numerical abundance, biomass) and anthropic disturbances. In investigated area 15 species of community interest were found while in the Form SCIs 20 ones are included. Concerning the ecological parameters used, in Alpine bioregion (ROSCI0381 and ROSCI0328) Cottus gobio and C. poecilopus were dominant while in Continental bioregion Barbus meridionalis and Romanogobio spp. were dominated. Regarding to anthropic impact, it have to be
    [Show full text]
  • A REVIEW of EXTANT CROATIAN FRESHWATER FISH and LAMPREYS Annotated List and Distribution
    Croatian Journal of Fisheries, 2019, 77, 137-234 M. Ćaleta et al. (2019): Extant Croatian freshwater fish and lampreys DOI: 10.2478/cjf-2019-0016 CODEN RIBAEG ISSN 1330-061X (print) 1848-0586 (online) A REVIEW OF EXTANT CROATIAN FRESHWATER FISH AND LAMPREYS Annotated list and distribution Marko Ćaleta1, Zoran Marčić2*, Ivana Buj2, Davor Zanella2, Perica Mustafić2, Aljoša Duplić3, Sven Horvatić2 1 Faculty of Teacher Education, Savska cesta 77, University of Zagreb, Zagreb, Croatia 2 Faculty of Science, Department of Zoology, Rooseveltov trg 6, University of Zagreb, Zagreb, Croatia 3 Ministry of Environmental Protection and Energy, Radnička cesta 80, Zagreb, Croatia *Corresponding Author, Email: [email protected] ARTICLE INFO ABSTRACT Received: 22 July 2019 A checklist of the freshwater fish fauna of Croatia is presented for the first Accepted: 26 September 2019 time. It is based on 1360 publications of historical and recent data in the literature. According to the literature review, there were 137 fish species in 30 families and 75 genera recorded in Croatia. The checklist is systematically Keywords: arranged and provides distributional data of the freshwater fish fauna as Danube drainage well as whether the species is endemic, introduced or translocated. Adriatic basin Endemism Introductions Translocations How to Cite Ćaleta, M., Marčić, Z., Buj, I., Zanella, D., Mustafić, P., Duplić, A., Horvatić, S. (2019): A review of extant Croatian freshwater fish and lampreys - Annotated list and distribution. Croatian Journal of Fisheries, 77, 137-234. DOI: 10.2478/cjf-2019-0016. INTRODUCTION the Dinaric karst region of Croatia (Banarescu, 2004; Smith and Darwall, 2006; Oikonomou et al., 2014) The Republic of Croatia is a small country with a harbours numerous endemic species that are found only land area of 56,594 km2 at the crossroads of several in Croatia, while some are also found in neighbouring European biogeographical regions: Continental, Alpine, Bosnia and Herzegovina (Ćaleta et al., 2015).
    [Show full text]
  • Endangered Fish Species in Balkan Rivers: Their Distributions and Threats from Hydropower Development
    Balkan Rivers Endangered Fish Species Distributions and threats from hydropower development 1 Balkan Rivers Endangered Fish Species Distributions and threats from hydropower development 1 Project Coordination & Writing Assoc. Prof. Dr. Steven Weiss, University of Graz, Institute of Zoology Universitätsplatz 2, A-8010 Contributions from Assoc. Prof. Dr. Apostolos Apostolou, Bulgarian Academy of Sciences Univ. Prof. Dr. Samir Đug, University of Sarajevo Dr. Zoran Marčić, University of Zagreb Dr. Anthi Oikonomou, Hellenic Centre for Marine Research Dr. Spase Shumka, Agricultural University of Tirana Univ. Prof. Dr. Predrag Simonović, University of Belgrade Dr. Daša Zabric, Hydrological Institute, Slovenia Technical Work (Preparation, Mapping, Layout, Artwork) M.Sc. Laura Pabst M.Sc. Peter Mehlmauer M.Sc. Sandra Bračun B.Sc. Ariane Droin Cover Page The upper Neretva River (A. Vorauer); marble trout (Salmo marmoratus) & Neretva spined loach (Cobitis narentana) (Perica Mustafić); map of distribution of the endangered softmouth trout (Salmo obtusirostris) Photo Credits Each photo is credited with the photographer’s name in the photo. For the following photographers, we additionally credit shutterstock.com: hdesislava, Dennis Jacobsen, Vladimir Wrangel, Rostislav Stefanek, Georgios Alexandris, Ollirg, Jasmin Mesic, Mirsad Selimovic, paradox_bilzanaca, Alberto Loyo, Alexandar Todorovic, bezdan, balkanyrudej, evronphoto, phant, nomadFra, Nikiforov Alexander, Irina Papoyan, Torgnoskaya Tatiana, Fesenko, Brankical, Sergey Lyashenko, Zeljko Radojko. Imprint This study is a part of the „Save the Blue Heart of Europe“ campaign (www.balkanrivers.net) organized by Riverwatch – Society for the Protection of Rivers (www.riverwatch.eu/en/) and EuroNatur – European Nature Heritage Foundation(www.euronatur.org). Supported by MAVA Foundation and Manfred-Hermsen-Stiftung. Proposed citation Proposed citation Weiss S, Apostolou A, Đug S, Marčić Z, Mušović M, Oikonomou A, Shumka S, Škrijelj R, Simonović P, Vesnić A, Zabric D.
    [Show full text]
  • Institute of Vertebrate Biology Academy of Sciences of the Czech Republic
    INSTITUTE OF VERTEBRATE BIOLOGY ACADEMY OF SCIENCES OF THE CZECH REPUBLIC 2007–2008 Biennial Report 2007–2008 Periodical continuation of the former Institute’s bulletins Vertebratologické Zprávy (1969–1987), Zprávy ÚSEB (1988–1991) and the ILE Biennial Report (1993–1994). Edited by Miroslav Čapek, Hana Slabáková and Jan Zima Published by the Institute of Vertebrate Biology of the ASCR, v. v. i., Brno Graphic design by Jan Dungel Printed by Metoda, spol. s r. o., Brno © Institute of Vertebrate Biology of the Academy of Sciences of the Czech Republic, v. v. i. ISBN 978–80–87189–04–7 CONTENTS Contents ........................................................................................................................ 3 Preface ........................................................................................................................... 5 Structure of the Institute of Vertebrate Biology of the ASCR, v.v.i. .............................................. 7 Addresses ....................................................................................................................... 8 Management and Services ................................................................................................. 9 Board of the Institute ...................................................................................................... 11 Supervisory Board .......................................................................................................... 11 Research projects ..........................................................................................................
    [Show full text]
  • European Red List of Freshwater Fishes Jörg Freyhof and Emma Brooks Published by the European Commission
    European Red List of Freshwater Fishes Jörg Freyhof and Emma Brooks Published by the European Commission This publication has been prepared by IUCN (International Union for Conservation of Nature). The designation of geographical entities in this book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of the European Commission or IUCN concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views expressed in this publication do not necessarily reflect those of the European Commission or IUCN. Citation: Freyhof, J. and Brooks, E. 2011. European Red List of Freshwater Fishes. Luxembourg: Publications Office of the European Union. Design & Layout by: Tasamim Design - www.tasamim.net Printed by: The Colchester Print Group, United Kingdom Picture credits on cover page: The European Mudminnow (Umbra krameri) is a European endemic found in the Danube and Dniestr basin. Its population has declined by more than 30% in the past 10 years due to river regulation and drainage of wetlands for agriculture which resulted in a reduced number of backwaters. This species is therefore classed as Vulnerable. Photograph © Andreas Hartl. All photographs used in this publication remain the property of the original copyright holder (see individual captions for details). Photographs should not be reproduced or used in other contexts without written permission from the copyright holder. Available from: Publications Office of the European Union, http://bookshop.europa.eu IUCN Publications Services, www.iucn.org/publications A catalogue of IUCN publications is also available.
    [Show full text]
  • Fishes of Montenegro
    Conservation Leadership Programme Final Report Fishes of Montenegro Survey of Endangered Fish Species of the Morača River System Fishes of Montenegro 2002 – Conservation Leadership Programme Conservation Leadership Programme Bronze Award 2002 Fishes of Montenegro Survey of Endangered Fish Species of the Morača River System Project leader: Radek Šanda Team Members: Jasna Vukić Danilo Mrdak Jörg Bohlen Sanja Stana Kaludjerović e-mail: [email protected] Final Report 2002 -2009 Geographical location: Montenegro, Europe 1 Fishes of Montenegro 2002 – Conservation Leadership Programme Summary A survey of the freshwater fish fauna of the River Morača system in Montenegro and an awareness campaign was carried out in the period 2002-2009. The field work was conducted in this drainage in July and September 2002 and in July 2003. Altogether 20 localities were sampled. To be able to reliably assess the taxonomic status of the fish species in the River Morača system, it was necessary to collect additional comparative DNA material from the surrounding areas (Albania, Bosnia and Herzegovina, Croatia, Macedonia, Serbia), which has been done subsequently in the period 2003-2008. We have recorded a presence of 28 species, of which 23 native. Eighteen of the native species are listed in the IUCN Red List, of which 14 are evaluated as Least Concern, one as Vulnerable, two as Endangered and one as Critically Endangered. The most important result of this study is a discovery and description of two new species of gobies, Knipowitschia montenegrina and Pomatoschistus montenigrensis. Further, one species was found for the first time in Montenegro (lamprey Lampetra zanandreai) and another for the first time in the River Morača system (introduced cyprinid Pseudorasbora parva).
    [Show full text]