Flora Mediterranea IO - 2000 381

Mediterranean chromosome number reports - IO edited by G. Kamari, F. Felber & F. Garbari

Abslracl

KamarL G., Fclbcr, F. & Garbari, F. (cd): Meditemll1ean çhromosomc numbcr reports - IO. -l'I. Medit IO: 381-430. 2000. - ISSN 1120-4052.

This is me lenm inSlalment or Il serìes or repons or çhromosome numbers from M«IilerranClII1 1IIl:a. peri-Alpine commumties and!hl: Atlanlic; Islands. in Frençh or English hmguage. (I com­ pr;scs çontribulions on 120 1a.'II: &1fr.~'Qliu, Delphm/Um. Muscm/ 3fld AII/um from Grccçe. by E. P. Barcka. M. Koutola & G. Kllmari (Nos. 1106(1109): RosuuJr/Q. Sedum. Umbili<;us. Aerhwl!ema. Af}·.uum. ArubidOlnis. Arobis, Aubrlefa. Aurmla. BlJCu/d/o. Bra.uica. Bunius. Cable, Calepmo, Capsella, Cardamme, Cardtuia, Camch/era, CJ)~oIQ , D/pIO/aris. ErfJI!hi/a. Eruea, Erysimum , Fiblgia, ba/is, upidium, Lobula"a. LI/llarm, Mal/lliola. Rupha/ll/s. Rapis/rum, Rorippa, Sinopis, Sis)'mbrlUm, raMa/ia. rh/aspi, Fumarla, GlauCi"",. Papal"t'r. Clema/is, Delphinium, Rammcu/us, Reudu and Saxifraga From Greece, by H. Runcmark (Nos. 1110- 1188): Genisla from Sardinia and Portugal. by T. Cusmn Velari, L Feo]; Chiapella & G. Bnçchenn (Nos. 1189-1190); Ge"lsla (rom Moroc,o and Dalcari, Islluuls, by T. eU'II'iI Vdwi, L. Feoli Chiapell a & V. Koso ~d (Nos. 1191-1192); Anel/usa, Pllimunoria and L)'copsis from Ilaly, Greeee and Turkey. by M. Bigazzi, G. Fiorini & F. Scl"i (Nos. 1193-1200); Atgl'/'OC)'/IsUJ, Chalfl(XC)'lisws, C)"IisUJ and ~"'Sta from Morocco, by H. Tahiri & P. Cubas (Nos. 1201-1207): 0 l'I0II1$ and Melllolus r,om Bulgatia. by D. Pa"IO\f1 &: A. Tosheo.'a (Nos. 1208·1212); Cu~x. C)'p"I'1IS, EkocharJ$, Sc-"pus, Sc-lfoenllS and rr/glachl1l from Bu lgaria. by M. Sloc"a (Nos. 1213-1226).

Addresses of the editors: Prof. G. Kamari. Depanment ofBiology. BOianicallnstitute, Un ivcrsity OfPalras. GR-265 00 Patras, Greece. Dr F. Felber, InstitUI de Botanique, Universile de Neuchàtel, eh. dc Chantcmerle 22. CH- 2000 Neuchiilel, Switzerland. Prof. F. Garbari, Dipanimento di Scienze Botaniche dell'Uni versità, via Luca Ghini 5, 1- 56126 Pisa, Italy, 382 Kamari, Felber & Garbari: Mediterranean chromosome number reports - IO

Reports (1l06-1I 09) by Eleftheria-Perdiko Bareka, Margarita Koutoula & Georgia Kamari

1106. Belle~'alla dubla (Guss.) Roemer & Schuhes - 2n "" 8 (Figs. la, b & 5a).

Gr: fonian islands, Nomos Zakinthou, Zakinthos island, elose to the villagc of Korithi, 37°54'N,200 42'E, 13 Oct 1991 , Philos & Kamari 26822 (UPA).

Be/levalia dubia is distributed in the C. and E. Mediterranean region. The chromosome number 2n "" 8 reportcd here confinns previous records from Greece, on material from Idhra island (Bothmer & Benzer 1973), and Turkey (Ozhatay el al. 1991). A triploid cnromosome nwnber of 2n - 12 nas also been reported for this taxon from Ilaly (Musano & Maggini 1976). The rnorpnology oflhe chromosome complernenl is in aCCOf­ dance with the generai "basic" Bel/{>va/ia. type (Bothmer & Wendelbo 1972), which includes one pair ofmetacentric (m), !wo pairs ofsubmetacentric (sm) and one pair ofacrocentric (st) chromosomes. Satellites are found on the short arrns of Ine mClacentric and on the long anns oflne submelacentric chromosomes. MicropnOlographs (Figs. la. b) and a karyogram (F ig. 5a) ofthe lonian popu lation srudied are presented here. The karyotype fonnula consist of211 = 2m-SAT + 4sm-SAT + 2s1 = 8 chromosomes, ranging in size from 12 to 6.7 I-un.

1107. Delphlnium !.-tuphi"sugriu L. - 2n >= 18 (Figs. 2a, b).

Gr: Peloponnisos, Nomos Achaias, elose lo the village of Metochi, piace named Strofilia with Pinus pinea foresl. in pnrygana, 38°0TN. 2 1°24' E, IO m, 25 May 1998, Phitul" & Ku",uri CII/t. No 8./5 (UPA).

De/phillium staphisagria is mainly distributed in Ihe Mediterranean region and is also found in the Canary Islands. The chromosome number 211 '"' 18 and the karyotype fonnula nave bcen reponed by Constantinidis & Kamari (1995, sec for references) in material from Makedonia and Sicrea Ellas and it is in accordance wilh our counts from Pelopoonisos. Moreove r, Simon et al. ( 1995) also reported the samc chromosome number in material from Spain. TIle karyotype is asymmetrical having one long melacentric (m) chromosome pair and onc submctacen­ Iric (sm) pair, while Ihe resI are acrocentric (SI) or subtcloscntric (t) (Figs. 2a. b). The kary­ otype fonnula is 211 .. 2m + 2sm + 10s1 + 41-SAT = 18, with chromosomes varying in size belween 16 and 2.7 }.1m.

1108. Muscar; macrocarpum Sweet - 2n "" 18 (Figs. 3a, b & 5b).

Gr: Easl Aegean Islands (EAe), Nomos Samou, Is l. Samos, W. rocky slopes of Mt Kerkis, E. ofthc piace known as Plaka, in margins of olive grovcs, sercc and grave­ Iy places, on li mestone, c. 450-650 m, 37°43 ' N. 26°35'E, 18 May 1999, Christodou/aki5 & Constanrillidis eu/t. no K285 (UPA).· (Fig. 3b) East Aegean Islands (EAe), omos Samou. Isl. Samos. MI Kerkis, ravine named Flora Mediterranea 10 - 2000 383

\

I la --.... ', lb

2a 2b

I

3a ,~ 3b

Figs. 1·3. Mitotic mctaphase plates or: l, B, b, HelJe~' alia dubia. 2n = 8: 2, I, b, Delphinium sfaphi. sagria. 2n = 18: 3, B, b. Muscari macrocarpum, 2n - 18. - Arrows indicate SAT-chromosorncs. Scale bar = IO ~rn. 384 Kamari, Fclber & Garbari: Mediterranean chromosome number rcports - IO

Kakopcrato, ca 500 m, 37"43'N. 26"3TE, 9 May 1984, Chrislodo/llakis 96 & 186 (UPA). - (Fig. l.) Easl Aegean Islands (EAe), Nomos Samou, Isl. Samos, dose lo the village Drakaioi, piace named Misomisi, ca 400 m, 37"45' , 26"36'E, 24 Apr 1976, Chrislodolllokis J47 (UPA). East Aegean Islands (EAe). Nomos Samou, Isl. Samos, N. of Pythagorio, hill named Spiliani. rocky places. 150 m, 37"4I'N, 26"56'E, 14 Apr 1999, Andreadakij.· cui,. no K340 (UPA). - (Fig. 5b)

Muscari macrocarpum is an E. Mediterranean elemcnt. In Greecc, il oceurs in the East Aegean islands. Kiklades islands and Kriti. 11 has been eonfused unlil rcecnlly with the taxonomically relatcd Muscari moschatum Willd., an endemie species of S. and W. Anatolia (Davis 1984). whieh also shares the same chromosome nurnber (Sluan 1970, Ùzhatay & Johnson 1996). The somatic number 2n = 18 of this species has previously been reported by Montmollin ( 1986) in material from Kriti and by Ozhatay & Johnson (1996) in material from Turkey. The same ehromosome number 2n = 18, microphotographs (Figs. 3a, b) and a karyogram (Fig. 5b) are presented here on material trom Samos island. The karyotype is symmetrieal, consisti.ng of2n = 10m + 6sm + 2sl-SAT " 18 ehro­ mosomes. The ehromosome size ranges from 7.9 to 2.7 Ilm. In populalion no K285 the satellites orthe homologous chromosomes show heterozygosity (Fig. 3b); also in popula­ tion no K340, the longest metacentric chromosome pair presenls an heterozygosity in the size of the two homologous ehromosomes (Fig. 5b).

11 09. Allium cQ llim;schon Link subsp. callimisclw" - 2n = 16 (Fig. 4).

Gr: Sierea Hellas. Nomos Elolias---Akamanias, MI Boum is tos, W. of Koumboti village. 500 m, 38"45 ' N, 21 °04' E, IO Oel 1998, V/achos 45/895 (UPA).

This Greek endemic spccies has been divided by Sleam (1978) inlo two subspecies. The typical subsp. callimischon is distribuled in Ihe mainland, while subsp. hacmusliclIIlII Sleam is moslly known from the island of Kriti . The chromosome number of 2n = 16 and a similar karyolype morpho!ogy have previ­ ous!y been reportcd by Stcam (1978), Johnson (1982), and latrou (1986) in materia! from Peloponnisos, by Tzanoudakis (1983) in materia! [rom Pe loponnisos and the 10n ian islands, and by Tzanoudakis et al. (1991) in material [rom Ipiros, Pcloponnisos and Kilhira island. Our eounl originating from Sterea Hellas adds a new record in lhc cytogcographi­ cal study ofthis taxon. The karyotype of the population sllldied is symmetrical, with mostly metaccnlric ehro­ mosomcs. Thc karyotype formula is described as 2n = 12m + 2m/sm + 2sm = 16. wi th chromosomes varying in size belween 12,5 and 10,9 Ilm.

Acknowltdgemtnt5

Thanks are due to allthose who kindly sent us material far cytologieal study. Financial suppon by thc Greek Minisny of Development. Generai Secretarial of Researeh and Flora Mediterranea lO - 2000 385

4

Fig. 4. Mitolic mcwphase plal( or ~Ihllm callinusc/1011 sllbsp. callil/li­ scholl. 211 " 16: - Scale bar = lO ~m.

m-SAT 'lsI s"m-SA T s"m-SA T a 'I Il Il " • .....1 'I m m sm m st-SAT sm m sm m b

Fig. 5. Karyograms of: a, Bel/n'olio dI/bio. 2/1 = 8: b, Muscori inacrocarpllm. 2/1 = 18. - Scale bar"'" lO ~m. 386 Kamari, Felber & Garbari: Mediterranean ehromosome number reports - lO

Technology (Projecl EPET Il , N.79) is gralefully acknowledged. Rtftrtn~ts

Bothmer, R. von & Bentzer, B, 1973: Chronl osome morphology in 8e/lewllia dubia subsp. boissù:ri nnd B. In/oliala (Liliaceae) from Greete. ~Ann , Mus. Goulnndris l : 11-13 . ~ & Wcnde\bo. P. 1981 : Cytologieal and morpbologieal variation in Bel/el'alia. ~ Nord. J. Bol. l : 4- 11. Constanlinidis, Tb. & Kamari, G. 1995: Repons (401 4 14). [In Kamari, G .. Felbcr. F. & Garbari. F. (cd.). Mediterranean chromosome number rcpons -5]. ~ Flora Mcditerranea 5: 265-278. Davis, P. H. 1984: Flora orTurkey and tbe East Aegean Islands 8: 248-249. - Edinburgh. Edinburgh University Prcss.. latrou, G. 1986: Contribution to tbc study of the endemie flora of Pcloponn isos. - rh.D. tbcsis. l'atras lin Greck. with English summar)' l. Jobnson, M. A. T. 1982: Kar)'otypes of some Greck spccies of A/lium. - Ann. Mus. Goulandris 5: \07-1 19. Montmollin de. B. 1986: Etude eytOlaxonomique dc la flore de la Crète [IL Nombres chromo­ somiques. - Cnndollea 41 : 43 1-439. Musano, L & Maggini. F. 1976: Triploidia in Bel/el'alia dubia (Guss.) ROc:me r & Scbultcs. - InCorm. Bol. haI. 8: 186. Olhatay, N. , Joh nsoll. M. A. T. 1996: Some karyological remarks 011 Turkish Allium seet. Allillm, Bel/elIalia, Aluscari, and Ornithogalum subg. Ornithogalum. - Ilocconca 5: 239-24 9. -, - & Mathew. B. 1991 : Cbromosome numbers ofTurkish 8el/elIalia species. inc1uding a n..:w hexaploid from Europcan Turkey. - I)ot. Chron. IO: 813-818. Simon, J., Bosch, M., Blanché, C. & Molero 1. 1995: Repons (481490). [In Kamari. G .. Fclbcr. F. & Garbari. F. (ed.). Mediterranean chromosomc number rcpons -5J. - Flora Mediterranea 5: 323-33 1. Stcam, W. T.. 1978: European spccies of Al/il/m and allied ge nera of Alli(lCf{/e~ a synonimic enu· meration. -Ann. Mus. Goulandri s 4: 83 · 198. Stuan. D. C. 1970: Chrornosome numbcrs in tbc gcnus Muscari Mill. - Notes Roy. Bot. Gard. Edinburg h 30: 189· 1%. Tzanoudakis. D. 1983 : Kar)'otypes of ten taxa of Allium ~c tion ScorodolT from GIl."ece. - Caryologia 36(3): 259-284. -. latrou, G., Kypriotakis, Z. & Cbristodoulakis. D. 1991: Cylogeogrnphical studies in some Acgean Liliaceae. - Bot. Chron. IO: 76 1-775.

Address ofthe autbors: E.-P. Bareka, M. Koutoula & !"rof. G. Kamari. Botanical Instit ute. Depanrncnt or Biolog)'. University of Patras, GR · 265 00 Patras, Hel las (G reece).

Reports (1110-1188) by Hans Runemark

The herbarium sheelS from whieh seeds were taken (colleclors and numbcrs in italies) as we ll as voucher specimens of cultivated p/ants (kept separately) are preserved al LD. For planlS raised from seeds eolleete

Snogeru p), St (Ame Srrid) and Sv (Linus Svensson). References to previously published chromosome studies for taxa treated are usually noi cited. For detailed infonnation of published counts see Jalas el aL , Alias Fl. Eu r.: voI. 8, 1989 (Rammculaceae), vol. 9, 1991 (Papaveraceae), val. IO , 1994 and val. I l, 1996 (Cruciferae), and vol. 12, 1999 (Resedaceae, Crassulaceae, Saxifragaceae).

Crassulaceae

1110. Rosular;a serrata (L.) A. Berger - 2n = 18.

o o Gr: E. Aegean islands. Ikaria, 4-5 km E. of Ag. Kirikos. 37 40'N, 26 20'E. 13 Jul 1958. R & S 12539.

1111. Sedum llispanicum L. - 2n = 14.

o Cr: E. Aegean islands. Ikaria, 2 km W. of viI. Plagia. E. exposed cliffs, 37 34'N, 26 <> 09'E, 14 Jul 1958, R & S 1257/.

1112. Sedum litoreum Guss . subsp. liloreum - 211 = C. 60.

o o Gr: Dodecanisa. ASlipalea, the island ofOfidousa, 36 33 'N, 26 09'E, 12 May 1960, R & N 13862. • • O" Klklades. Naxos, the N. Islet al Akr. Pardenos, 37 02'N. 25 22'E. 2 Jul 1967. R & Be 30737.

1113. Sedum rubem L. subsp. rubens - 2n = c. 84.

o o Gr: Kiklades. Kato Koufonisi (S. ofNaxos), 36 55'N, 25 34'E, Il Jun 1960, R& N 15854.

1114. Sedum sediforme (Jacq.) Pau - 2n = 48 .

o o Gr: Kiklades. 10s, N. ofthe harbour, 36 44'N, 25 I7'E,5 May 1957, R 2332.

1115. Umbilicus clliorantllus Boiss. - 2n = 48.

o o Gr: Evvia. N. of Ag. Dimitrio, 38 08'N, 24 27'E, 23 Jun 1958, R & S 1/ 82 1.

To my knowledge, Ihe first chromosome. report for the species.

1116. Umbilicus rupestris (Salisb.) Dandy - 2n = 48.

Gr: Evvia. 3 km W.S. W. of Akr. Kafirefs, 38 <> 08'N, 24"33 'E, 22 Jun 1958. R & S seeds, 388 Kamari, Felber & Garbari: Mediterranean chromosome number reports - IO

cult. R-1 308.

Cruciferae

111 7. Aelhlon emo saxalile (L.) R. Br. subsp. crelicum (Soiss. & Heldr.) l. A. Andersson etal.-2n = 24.

G r: E. Aegean islands. tkaria, Ag. Nik.olaos (S. of Evthelo), 37°37'N, 26°09'E. 24 Apr 1958, R & S 6809. Kik.lades. Donousa (E. of Naxos), innermost part of Ormos Rousa (Kalotaritissa), o o 37 07'N,25 49' E, 23 May 1958. R & S 9438; d:o, 25 May 1958, R & S 9605; Naxos, . o o AmmomaxlS Oros, c. 800 m, 37 07'N, 25 33'E. 31 May 1958. R & S 9927, R & S o o 9949; Naxos, Fanari Oros, 700 m, 37 05'N. 25 30'E, 31 Jun 1958, R & Sseeds, eu/t. o o R-1250; Naxos, 2 km N.N.W. ofOnnos Leonis, 37 09' N, 25 35'E, 21 May 1958, R & S 9070; Naxos, Metri (N. of Moni), 37°05'N, 25°30' E, 20 May 1957, R 3/77; Na.xos, N. ofMirria (Engares), 37°06'N, 25 °26' E, 26 lui 1958, R & Sseeds. CII/t. R· • o o 1289; Naxos, Slavros Keramotls, 37 06' N, 25 32'E, Il lun 1957. R 445/ ; Paros, mI. o o Prof. Elias, 700 m, 37 02 ' N, 25 12 'E, 18 Jul 1958,.R & S seeds, CII/t. R-1 376.

111 8. A/yssumfollosum Bol)' & Chaub. - 2» = 16.

Gr: Aniki. Gerania Oros. E. of the peak area, c. 1000 m, 38°01 'N, 23°09 'E, 20 May 1982, R & Sv 48929.

111 9. A/yssum slmplex Rudolfi ("A . campestre subsp. campestre") - 2n "" 16.

. o o Gr: Klk1ades. Kea, N. ofOnnos Ka logeros, 37 37'N, 24 17'E, 17 Apr 1969. R. SI, & G o o 40812; Kea, E. or Ag. Theodoro, 37 34'N,24 19'E, 15 Apr 1969, R. SI & G 40670: o o Kithnos, the valley N.E. of Ag. Louka, 37 26'N. 24 23 ' E. 3 Jun 1968, R & E 379/3.

1120, Alyssum simula" s Runemark ined. - 2n = 32.

Gr: Argolis. Arachnion Oros, the W. part and the peak area, 600-1 200 m. 37°39' N. 22°58' E, 14May 1982. R&Sv4866/. Attiki. Gerania Oros, E. ofthe peak area, c. 1000 m, 38°Ol'N, 23°09' E, 20 May 1982, R & Sv 48925; Gerania Oros. the peak area, 1300-1315 m, 38°01 ' N, 23°08' E. 20 May 1982, R & Sv 4892/a.

A. simulans is in several respects intennediate between A. foliosum BOf)' & Chaub. (2n = 16) and A. minutum DC. (2n - 16) and may well be an allotetraploid based on these IWO Flora Mediterranea IO - 2000 389

species. [t is a mOnlane Greek endemie known from Argolis, Sterea Ellas and Kriti. Il will be formally described in the fonhcorning vol. 2 of Flora Hellenica.

1121. A/yssum slrigosum Banks & Solander - 2n = 16.

• o o Gr: Dodeeanisa. Karpathos, the island ofSaria, N. ofOnnos Ammo, 35 50'N, 27 14 'E, 4 May 1958, R & 5 7656.

1122. Alyssum umbellatum Desv. - 2n = 16.

o o Gr: E. Aegean islands. 1karia, S.w. of"Kaka Rafija", 37 33'N, 26 O9'E, 15 Ju1 1958, R & S_seeds, cu/I. R-1394. o o Kiklades. Tos, Ag. Theodotis, 36 47'N, 25 18'E, 22 Apr 1969, R, 5r & G 41048: o o Mikonos, Onnos Ftelia, 37 28'N, 25 23'E, 12 May 1968, R & E 3533/; Mikonos, the va lley S. of western mI. Ag. Elias, 37°28' N, 25°20'E, 13 May 1968, R & E o o 35396; Mikonos, the S, part ofeastem mI. Ag. El ias, 37 2TN, 25 27'E, 17 May 1968, R & E 36230; Mikonos, the island of Dilos, C. part, 37°22'N. 25° 16·E. 15 o o May 1968, R & E 35758; Kithnos, the valley N.E. of Ag. Louka, 37 26' N, 24 23'E. 3 Jun 1968, R & E 37912.

1123. Arabldopsis thaliana (L.) Heynh. - 2n = IO.

Gr: E. Aegean islands, Ikaria, 1 km N. of western Ag. Nikolaos. 37°32'N. 26°04'E, 21 Apr 1958,R& 56153. Kiklades. Mikonos. belween Lino and Ano Mera, 37°27'N, 25°24'E, 27 Mar 1969, • o o R, 5f & G 39531 ; Naxos, Faneromem, 37 09'N, 25 29'E, 22 May 1957, R 3394; Naxos, 1 km E. ofStavros KeramOlis, 37°06'N, 25°33'E, 31 Mar 1958, R & 54567; Serifos, Livadia, 37°09'N, 24°31'E, 26 ApT 1967, R & Be 27595; Sifnos, S. of o o Kastro, 36 59'N,24 45'E, IO ApT 1969, R. SI & G 40426.

1124. Arabis verna (L.) R. Br. - 2n = 32.

o o Gr: Kiklades. Naxos, Metri (N. of Moni), 37 05'N, 25 30' E, 13 Jun 1957. R 3213; Naxos, Stavros Keramotis, 37°06' N. 25°32'E, l3 Jun 1957, R seeds, culI. R-15 3.

1125. AuhTieta deltoidea (L.) DC. - 2n = 16,

Gr: E. Aegean islands. Samos. Mt Kerkis, 1200 m. 37° 44'N. 26°37'E, 2 Aug 1960. R & N /6925. 390 Kamari, Felbcr & Garbari: Mediterranean chromosome number reports - IO

1126. AUT/n/a saxotJ/Lf (L.) Oesv. subsp. orienta/is (Ard.) T. R. Dud ley - 2/1 = 16.

. 0 . ° Gr: Arkadm. 3 km S. or Astros, 37 23 N, 22 45' E, l May 1969, R & Be ./1527.

1127. Biscutella didymo L. - 2" = 16. e r: Sit ia. Above Ori, 450-700 m, 35 •09'N,26 • 07 ' E, 12 May. 1962, R & S / 72 /./. G r: Aniki. 4 km S.E. or Markopoula, 37 • 52 'N, 23 •58 ' E, 12 May 1963, S 20/2/. Dodecanisa. Astipalea, Maltesana to Vriseu Punda, 36°J5' N, 26°25'E, Il May 1960. R & N /3579; Astipalea, the island ofOtidousa, 36°33'N, 26°09' E, 12 May 1960, R o • & N 13 712; Dio Adelti (N.W. or Sirina), the E. islet, 36 25 'N, 26 38'E. 14 May 1960, R & N 14077; d:o, the W. islet, 36°25'N, 26°37'E, 14 May 1960, R & N 1./ 188. Kiklades. Amorgos. Katapola, 36°50'N, 25°52'E, 15 Apr 1957, R /222: Ant imilos. 36°48'N, 24° 15'E, 15 May 1958, R & S 8755; Ano Koufonisi (S. of Naxos). 36560 , N, 25 °36'E, 14 Apr 1957. R 1070; Klmolos. , S. and S.W. or thc harbour, 36°47'N,24°35'E, 17 Apr 1967, R & Be 258/0; Kimolos,thc i51et or Ag. Gcorgios, 36• 45 ' N,24 •34'E , 17 Apr 1967. R& Be 25657: Milos, Akradi, thc E. islet,36• 45 ' N, 24°24'E, 2 1 Apr 1967, R & Be 26824; Milos, Kastro, 36°44 'N, 24°25'E. 20 Apr 1967, R & Be 26504: Mikonos, the valley S. or western mt. Ag. Elias, 37°28 ' N, 25 •20'E, 13 May 1965, R & E 3536/; Naxos 2 km E,N. E. or Skado, 400 m, ° ° . ° 0 ... 37 OS 'N. 25 33'E, I Jun 1957, R 3791; Naxos, E.N. E. orMoni, 37 05 ' , 25.,,0 E, 30 May 1957, R 3574; Paros, Pankla.. , 37 °05 ' N, 25 °09 ' E, 5 Apr 1967, R & Be 24/50: Policgos, the W. shore inside the islet, 36• 46'N , 24 •37'E, 19 Apr 1967, R & Be o • 2639/; Scrifos, berween Livadia and the town. 37 09'N, 24 30'E, 26 Apr 1967. R & Be 27353; Slkmos. . , N. or Kastro. 36° 42 'N. 25 °OTE, 9 Apr 1967, R & Be 24553; Siros, Oros Kapari , 37°27 'N, 24°56'E, 7 Apr 1958, R & S 510/; Thira (Santorini). thc island ofNca Kaimcni, 36°24 'N, 25°24'E, 19 Ap r 1967, R & Be 27/73.

1128. BTosslca lournefortl1 Gouan - 2n = 20.

G r: Kiklades. Mi los, Chivadolimni, 36°41 ' N. 24°27'E, 20 Apr 1967, R & Be 27254; Si· ros, lahssas,, 37 o26'N, 24 °53'E. 30 May 1968, R & E 3 76 92.

1129, Bunlas erucagQ L. - 2n = 14 .

Gr: E. Aegean islands. Ikaria, Kampos, 37°38' N, 26° IO'E, 25 Apr 1958, R & S 690$8. Klkl· ades. Naxos, 2 km N.E. of Sangn,. 37 °03 ' N, 25 °28'E , 18 Apr 1958, R & S 5856; Naxos, between Moni and Sirones, 500-700 m, )7°05 ' N, 25°20' E. IO Ma)' 1968, R Flora Mediterranea IO - 2000 391

& E 3501/; Serifos, Koutalas, 37°08 'N, 24"'27' E, 28 Apr 1967, R & Be 17969.

1130. CokiJe maritima Scopo - 2n = 18.

o o Gr: E. Aegean islands. lkaria, Faros beach, 37 40'N. 26 21 'E, 13 luI 1958, R & S 11503. o o Kiklades. Antiparos, near the vi llage, 37 02'N, 25 05'E, 17 May 1967. R & Be o o 18837; 10s, Orrnos Tris Klisies. 36 40'N, 25 23'E, 26 luI 1967, R & Be 30178; Milos, Aliki, 36°42 ' N, 24°28 'E, 19 Ju l 1967, R & Be 19820; Tinos, Platia Ammos, 37°33'N, 2S "08'E, 18 May 1968, R & E 36360.

1131. Calepina irregularis (Asso) Thell. - 2n = 28.

Gr: E. Aegean islands. lkaria, N. ofKampos. 37"38 'N, 26" 10 'E, 25 Apr 1958, R & S 6926.

1132. Capsella bursa-pastoris (L.) Medie. - 211 = 16.

o o Gr: Kiklades. Ano Koufonisi (S. of Naxos), 36 56'N, 25 36' E. 14 Apr 1957, R 1063; Naxos, mI. W. ofAxapsis, 37"07'N, 25°25'E, 27 Mar 1957, R 488.

For morphology and chromosome numbers see Svensson (1984).

1132a. Capsella bursa-pastoris (L.) Medie. - 2n = 32.

Gr: Kiklades. Kimolos, between the town and the harbour, 36"48' N, 24"35 ' E, 17 Apr 1967, R & Be 25855.

1133. Cardamine hirsuta L. - 211 = 16.

Gr: E. Aegean islands. Ikaria, above the western Ag. Nikolaos, 300-500 m, 37"32'N, 26"04 'E, 21 Apr 1958, R & S 6232. o o Kiklades. Sifnos, 2 - 3 km S. of Akr. Khondropo, 36 57'N, 24 41'E, 12 Apr 1967, R & Be 25280.

1134. Cardamine graeca L. - 2n = 18.

Gr: E. Aegean islands. Ikaria, above western Ag. Nikolaos. 37"32'N, 26"04 ' E, 21 Apr 1958, R & S 6137.

1135. Cardaria draba (L.) Desv. - 2n = 64. Gr: Kiklades. Milos, the bayN.N.E. ofmt. Pro f. Elias. 36°41 'N, 24"23'E, 19 Ju n 1967. R & Be 1989/. 392 Kamari , Felber & GarbaTi: Mediterranean chromosome number reports - IO

1136. Carr;chle, a annua (L.) DC. - 2n = 16.

Gr: Attiki. The S. islet E. oflpsili (N.E. ofEgina), 37<>48' N, 23 <> 18'E, 17 May 1974. R & Bo 47456 b.

1137. Clypeolajonthlaspi L. subsp. jonthlaspi - 2n = 32.

G r: Kiklades. Amorgos. Ayiali to Tourlaria., 36 <> 55'N, 25 <> 59'E, 25 Apr 1969. R, SI & G 41289; los, P5ati s, 36<>45'N, 25 <> 22'E, 22 Apr 1969. R, SI & G 4/090: Naxos. Or05 o • o Zeu5, 450-600 m, ]7 O,, ' N. 25 29'E. 6 Apr 1957. R 79/; Paros. above Levka. 37<> 03'N, 25<> 12 ' E. 6 Apr \967, R & Be 244/3; Siros , ~Oros Kapari. 37°27'N. o o o 24 56'E, 7 Apr 1958, R & S 5245; Siros, Kastri, 37 30'N, 24 56'E, 3 Apr 1969. R, SI & G 40044. Lakonia. MI Taygetos, N. W. of Ama, c. 1000 m, 36 <> 53'N, 22 <> 23'E, 2 1 May 1964, R & S 20586.

1137a. C/ypeo/ajonth/aspi L. subsp. microca,pa (Moris) Archangeli - 2" = 16.

Gr: E. Acgcan islands. Ikaria, N. ofwestem Ag. Nikolaos. c. 300 m, 37°32' N. 26<> 04 ' E. 2 \ Apr 1958, R & S 6204. 9 Kiklades. Kea, N. ofOnnos Kalogeros, 37°37'N, 24 17' E, 17 Apr 1969. R, SI& G 9 9 40809; Naxos; the pass between Filoti and Apirantos, 37 03'N, 25 30·E. 19 Mar o o 1957, R 200; Naxos. N. ofthe monaslery ot"Chrisoslomos, 37 07'N, 25 24 ' E, 16 Mar 1957. R 79.

A report of2n '" 32 from Iran by Ayravand (1975) needs con finnalion.

1138. Diplolaxil' l'iminea (L.) DC. - 2" = 20.

9 G r: Dodecanisa. The smal! island of Astakida (N. of Karpathos), 35°53'N. 26 50' E, 8 May 1967,R& Be 28452. Ki kladcs. Paros, the isici ofGlaropounda, 36°59'N, 25°06'E. 17 May 1967, R & Be 29004.

11 39. Erop"J/a macroca'pa Boiss. - 2n = 24.

G r: Kiklades. Tinos, Koris Pirgos, 400 m, 37°38'N, 25°04 'E, 2 Apr 1969. R, SI & G 39983. To my knowledge me firs t cnromosome report for the specics.

1140. Erophila ,'erna (L.) Chevall. subsp. verna - 2n = c. 36. Flora Mediterranea IO - 2000 393

o o Gr: Ki kl ades. Tinos, 1 km N.E. ofFalatados, 37 36'N, 25 12'E, 29 Mar 1969, R. SI & G 39689.

1140a. Erophila verna (L.) Cheval1. subsp. praecox (Steven) Vollmann - 2n = c. 34.

o o Gr: Kiklades. Mikonos, western mI. Ag. Elias. 37 29'N,25 20'E, 28 Mar 1969, R. SI & G 39592.

11 4 1. Eruca vesicaria (L.) Cav. - 2n = 22.

Gr: Klklades... SlroS , Ag. Varvaras, 37 °28 ' N, 24 °54'E , 30 May 1968, R & Bo 37582: o o Tinos, between Koli bitras and Komi , 37 37'N, 25 08'E, 23 May 1968, R & E 37027.

1142. Erysimum hayekii (Jav. & Rech. fiL) Polalschek - 2n = 14.

G r: Kìklades. Naxos, Stavros Keramotis, 600 m, 37°06'N, 25°31'E, 13 May 1957. R seeds, cl/lt. R-57.

1143. Erysimum rllOdium Snogerup - 2n = 12.

o o G r: Dodecanisa. Rodos, cliffs S.E. ofSalakos. 300 m, 36 16'N, 27 57'E, 2 Jul 1967, R & Be 29372; Rodos, S.w. of Siana, cliffs 380 m, 36°08'N, 27°47'E, 2 lui 1967, R & Be 29395. Erysimum senoneri (Heldr. & Sart.) Snogerup subsp. amorginum Snogerup - 2n = 12.

o o G r: Kiklades. Amorgos, the island ofNikouria, 36 53'N, 25 54'E, 30 luI 1967, R & Be 30580.

1145. Fibigia lunarioides (Wi11 d.) Sibth. & Sm. - 2n = 16.

o Gr: Dodecanisa. Chalki (N. of Rodos). 4 km N.W. of the harbour village, 36 14'N, o 27 35'E, 15 lun 1966, Bo 23000; Kinaros (E. of Alllorgos), N. exposed cliffs. 36°59'N, 26°17'E, 3 Jul 1958, R & S 12111; Mavronisi (islet between Kinaros and Lev.ltha) , 36° 59'N, 26 0 20 , E. 2 luI 1958, R & S 12070. Kiklades. Folegandros, 2 km W. ofPelagia, 36°36'N, 24 °56 'E, 15 Jun 1967, R & Be . . ° o 29526; los, Akr. PlrgaTl, 36 39'N, 25 23'E, 26 l un 1967, R & Be 30/93; Keros (S. ofNaxos), the island of Antikeros, 36°57'N, 25°29'E, 6 lui 1958, R & S 123 78. Endemie to the C. and S. Aegean areas and confined to coastal cl iff s and ungrazed is lets. To my knowledge, the first chromosome repolls for the species. 394 Kamari. Fe lber & Garbari: Mediterranean chromosomc number repons - lO

1146. Iratis lusitanica L. - 2n "" 14.

o o Gr: Dodecanisa. Karpathos. E. or Vurgunda, 35 48' N, 27 Il 'E. 7 May 1967, R & Be 28356.

11 47. Lepidium lpinosum Ard. - 2n - 24 .

Cl': Kik lades. Mikonos, the bay ofElia, 37• 28'N, 25 •24'E. 12 May 1968. R & E 35251: • o o Mlkonos, N. of Ano Mera, 37 2TN. 25 24'E. 12 May 1968. R & E 351 77,

To my know ledge Ihe fi rs l chromosornc reports for this spec ies,

11 48, Lobularia IIbyca (Viv.) Webb & Berth. - 2n - 22.

Cl': Kiklades. Thira (Santorini), near the lown, 36°25'N, 25°26'E. 16 Apr 1967, Il 26989; d:o, 22 Apr 1967, H 27250.

Apparcnllyan introduced and establishcd weed on Thira (fi rsl collected in 19 11).

1149, Lu"aria annua L. subsp. paclty rlriza (Borbas) Hayek - 2n = 30.

0 Gr: lonian islands. Kerallinia, above Sami, 38° 15'N, 20 39'E, 9 Jun 1966, S 236/2.

liSO. Malthiola rùluata (L.) R. Br. - 2n = 14.

Gr: Dodecanisa. Karpalhos, 3 km N. of Diafan i, 3So46' N, 27° I3 ' E, 15 Jul 1966. Bo 23181 . E. Aegean islands. Ikaria, Praia P:t, 37°34'N, 26°09' E, 171un 1958, R & S 1/34 7; Q Ikaria, N.W. or Praia P:t, 37 34'N, 2l09'E, 17 lun 1958, R& S 11352. Evvia. Peta lides, the islel of Prassonisi, 3S002'N, 24°15'E, 2 1 Jun 1958. R & S 1/562. Kiklades. 105, Varvaronisi, the largcr islel, 36°39'N, 25°23'E, 26 lui 1967. R & Be 30202; Kcros (S. ofNaxos), thc islct of Plaki, 36°S2'N. 25°31'E, IO lun 1958, R & S 10947; Makares, the .lslet of Strong .llo, 37° 04'N, 25 °42'E. 5 Jun 1958, R & S o • 10343; Milos.Akr. Rom ma, 36 42'N. 24 33' E, 16 lun 1967. R & Be 2962/; M il os. Q Ananes, the N.W. is let, 36 3 1'N, 24°0S'E, 17 lun 1967, R & Be 29764: Poliegos, Q Ihe islel al the N. shore. 36 47'N, 24 °31'E, 16 Jun 1967, R & Be 29608: Poliegos, the N. shore, 36 •47'N, 24• 37'E, 16 J un 1967, R & Be 29602.

1151 . l'tlallhiola tricuspidata (L.) R. Br. - 2n = 14. Flora Mediterranea IO- 2000 395

o o Cr: Sitia. S.w. ofLeopetra, 35 13'N, 26 0 1' E, 18 May 1962, R & S /8491. o o G r: E. Aegean islands. Ikaria, Faros, 37 40'N, 26 21'E, 13 Ju l 1958, R & S 12476. Kiklades. Antiparos. the islet of Kavouras, 37°04'N, 25°05 'E, 3 Ju l 1967. R & Be Q 30835: lraklia (S. ofNaxos), S. ofthe harbour. 36°50'N, 2S 29'E, 7 Jun 1957, R o o 4205; Kea. Ozia, 37 41'N, 24 2 1' E, 4 Jun 1968, S & Bo 3423 l; Kithnos. the valley N.E. or Ag. Louka, J7~ °26'N, 24 ° 2 3' E, 3 Jun 1968, R & E 37895: Mtkonos. , Onnos Omos, 37 °25 'N, 25 ° 19' E, 14 May 1968, R & E 35505; Naxos, 3 km N. of Mt.t n.a o o (Engares), 37 08'N, 25 26'E, 18 May 1957, R 3/04: Naxos. E. of Apollona. 37°1 l'N, 25°33'E, 5 Jun 1957, R 4/07; Siros, the islet or Ampelos (S. or Vari). 37°23'N, 24°58'E, 3 Jun 1968, S & Bo 33498.

1152. Rapllanus rapltalllsirum L. - 2n = [8.

Gr: Kiklades. Mikonos, the bay of Elia, 37°25'N. 26°0 1'E, 17 May [968, R & E 36267; Mikonos, tbe is land or Rinia, centrai pan, 37°25 ' N, 25 ° 13 ' E, 25 Mar o o 1969. R. St & G 39295; Naxos tbe beacb S. oftbe lown, 37 06' N,25 23 ' Et 4 May 1957, R 2151; Naxos. Stavros Keramotis, 500 m, 37°06' N, 25°31' E, I I Jun 1957, R4461.

11 53. Rapistrum rugosum (L.) A Il. - 2n = 16.

Gr: Dodecanisa. Sirilla (S.S.E. ur A~lipa!t:a), S. ur tht: sma ll vil[agc. 36°20'N, 26°41 'E, 2 May 1958, R & S 7309; Levi tha. "Pono di Levitha", 37°00'N, 26°26'E, 2 lui 1958, R & S 1/993. Kiklades. Sifnos, Ormos Kondos, 36°54'N, 24°43 'E, 13 May 1958, R & S 8508: o o Naxos, N. of Ag. Sofia, 37 10 ' N, 25 29' E, 3 Apr 1958, R & S ./985.

1154. RoripplI icarica Rech. fil. - 2n = 16.

G r: E. Aegean islands. Ikaria, MI Alhcras. E. pan, c. 800 m (Iocus classicus), 37°38' N, 26° 16'E, 12 luI 1958, R & S 1244/: Ikaria, N. ofwestem Ag. Nikolaos, 300-400 m. 37°32'N, 26°03 'E, 16 Jun 1958, R & S 11267; lkaria, Mt Melissa, c. 800 m. 37°32'N, 26°05 ' Et 15 Jul 1958, R & S /1638.

Endemie to the island or Ikaria. To my knowledge, me firsl chromosome reports for the spec ies.

11 55. Rorippa naslurlium-aquulicum (L.) Hayek - 211 "" 32. 396 Kamari, Felber & Garbari: Med iterranean chromosome number reports - lO

Gr: Ki klades. Kit hnos. Merixas. 3{22'N, 24°24'E, 2 Jun 1968. R & E 38020; Naxos, o o E. of Apo llona, 37 Il 'N, 25 33'E. 4 Jun 1957, R 398/; Naxos, I km E.N.E. of Skado, 37°08'N, 25°33'E, I Ju n 195 7, R 3832; Serifos, the shore near the harbour, )7°09'N, 24°31'E, 20 Jun 1967, R & Be 30062.

1156. Sinapis alba L. subsp. millrel (H. Lindb.) Maire - 2n = 24 .

o o Gr: Kiklades. Makares (E. or Naxos), the is let of Strongil0, 37 04 ' N, 25 42'E, 5 Jun 1958, R & S 103 70.

11 57. S lsymbrium Ido L. - 2n - 14.

Gr: Klk. lades. SlroS,. near the town, 37 °27'N, 24 °56'E, 30 Jun 1968, H 37545.

1158. S lsymbrlum orientale L. - 2n = 14.

Gr: Kiklades. Mikonos, N. orOrmos Omos, 37°26'N, 25°19' E, 18 Jun 1960, R & N o o /6/02; Siros, me islet orStrongilo (E. ofDidimi), 37 26'N, 24 59 'E, 27 May 1968, S & Bo 33389; Skinousa (S. of Naxos), between the village and me harbour. 36°52' N, 25 °31'E, 9 Jun 1957, R 4317; Thira (Santorini), the island ofThirasia, o o between the harbour and Manolas, 36 26'N, 25 21 'E, 27 Jun 1967, R & Be 30332; Tinos, S. ofthe town, 37°32 'N, 25°1D'E, 19 May 1968, R & E 36507.

1159, S il'ymbrium polyceratium L. - 2n '"' 28.

o o Cr: Sitia. S. ofthe town of Sitia, 35 II 'N, 26 07'E, 15 May 1962, R & S 1792/. Gr: Kiklades. Siros, near the town, 37°27'N, 24°56' E, 30 May 1968, H 375./3; Th ira o (Santorini), the island of Thirasia, between the harbour and Manolas, 36 26'N, 25°21 'E, 27 May 1967, R & Be 30312; Tinos, below ISlernia, 37°3TN, 25°03'E, 26 May 1968, R & E 37243.

1160. Teel'dalia coronopifolia (J. P. Bergeret) Thell . - 2n <= 36.

Gr: Klk. lades. Naxos. 3 km S. or AxapSlS,. 37 °07 ' N, 25 °24 'E, 26 Mar 1957, R 408.

1161 . rh/aspi bulbosum Boiss. - 2n = 14.

Gr: E. Aegean islands. Ikaria, mI. Melissa, near the peak, 930 m, 37°32'N, 26°05'E, 12 Jul 1958, R & S secds, culto R· 1228. A very distinct, montane Greek endemie, related lO Th/aspi arlanricum Balt. from Algeria. Known from a few loca lities in Sterea ElIas, Evvia, and the E. Acgean islands Flora Mediterranea IO - 2000 397

(Ikaria and Chios). To my know ledge, {he fi~ t chromosome repon for the species.

11 62. Thlal"p; pe,folitllum L. subsp. pe'foJJa1um - 2n:o 42.

Gr: E. Aegean islands. Jkaria, N. of western Ag. Nikolaos, gorge 300-500 m. 37"32'N, 26°04'E. 21 Apr 1958, R & S 6252. o o Kik lades. Naxos, Oros Zeus, 900 m, 37 02'N, 25 05'E, 14 May 1957, R seeds ,clI/I. . o o R-178; Naxos, N.E. ofMonl, 700 m, 37 05'N, 25 30'E, 31 Jun 1958, R & Sseeds, cult. R-1317.

Papaveraceae

11 63. Fuma,ia judaJca Soiss. - 211 .. 48.

, o Gr: Kik lades. Anafi, Ka1amos, 36 22'N, 25 50'E. 8 May 1958, R & S 8097; Mikonos. 0 , o . 2 km N. of Ag. Stefano, 37 30 N. 25 19' E, 17 May 1%8, R & E 36166; Mlkonos. o o 1-2 km N.N.W. of Ano Mera, 37 28'N, 25 23'E, 12 May 1968, R & E 35147: o o Naxos, 2 km N.N.W. ofKalo POlarnia, 37 05'N, 25 25 'E, 2 Apr 1958, R & S 4804: Naxos, S. of the town, 37°06'N, 25°23 'E, 15 Apr 1958, R & S 5536.

11 64. Fuma,ia macroca'pa Pari. - 2n - 16.

Gr: E. Aegean islands. Ikaria, westem Ag. Nikolaos, 37°3 1'N, 26°0:3"13, 20Apr 1958, R &S607/. o o Kiklades. Naxos, Oros Zeus, W. slope,400 m, 37 02'N, 25 29'E, 16 Apr 1958, R & o o S 5743: Tinos, between Mousoulo and Livadia, 37 35'N. 25 13 ' E. 22 May 1968, R & E 37020,

11 65. Fuma,ia officinalis L. - 2n ~ 32.

Gr: Kiklades. Naxos. W. of Mt Prolì lis Elias, 37°01 'N, 25°28'E. 21 Mar 1957, R 290; . o , ° Naxos 3 km W. ofAxapsls, 37 07 N, 25 24'E, 26 Mar 1957. R 389.

11 66. Fuma,ia pener; Reichenb. - 2n = 32.

Gr: Kiklades. Naxos, Onnos Leonis, 37°09'N, 25°35 ' E, 2 1 May 1958, R & S 9072: o o Naxos, Oros Zeus, W. part, 400 rn, 37 02'N, 25 29'E, 16 Apr 1958, R & S 56/9; o o Naxos, N. of Ag. Sofi a, 37 09'N, 25 28'E. 3 Apr 1958, R & S 4956.

11 67. Glauciumj1avum Crantz - 2n - 12. 398 Kamari, Felber & Garbari: Mediterranean chromosomc number reports - IO

Gr: Kiklades. Kea. I km S. W. ofthe islet ofSpanopoula, 37°41 'N, 24°22'E. 4 Jun 1968. S & Bo 34290.

1168. Papaver apulum Ten. - 2n= 12.

o o Gr: Kiklades. Kea, S.E. of the vi llage of Kea. 400 m, 37 28'N. 24 21'E, 1 Jun 1968, S. Bo & E 33957.

11 69, Papa ver argemone L. subsp. I/igrotinctum (Fedde) Kadere it - 2n = 42.

Q G r: Kik ladcs. Mikonos, Onnos Omos, 37 25'N, 25°19'E, 17 Jul 1960, R & N 16082 : o o Kithnos, thc vall cy N.E. ofAg. Louka, 37 26 'N, 24 23'E, 3 Jun 1968, R & E 37909.

The plants from both localities have been detennined by Kaderci t. Only 2/1 - 14 has prc­ viously been reported for this subspecies.

11 70. Papal'er dubium L. subsp. dubium - 2n = 42.

o o Gr: Kiklades. Naxos, Ammomaxis Oros, 1 km E.S.E. of Votri, 37 07'N, 25 33' E. 31 May 1958, R & S 9895.

11 71. Papaller IIybridum L. - 2n = 14 .

Q Q G r: Kikladcs. Naxos, 2 km S.W. ofAxapsis, 37 08'N , 25 24' E, 30 Jul 1958. R \( S o o seeds, CII/t. R- 1537 ; Kato Koufonis i (S. of Naxos), E. part, 36 55'N. 25 34'[, II Jun 1960, R & N /5866.

11 72. Pap aw!r purpureomarginQtum Kadereit - 2n = 28.

o Gr: Dodecanisa. The is[et or Khamili (between Anafi and Karpathos), 35 52'N, 26°14'E, 4 May 1967, R & Be 28 f.18.

11 73. Papaver r/lOeQs L. - 2n :::: 14.

Gr: Dodccanisa. Astipalea, belween Maltcsana and Vriseu Punda, 36°35'N. 26°25'E. Il May 1960. R & N lJ474; Astipalea. the island ofOfidousa, 36°33'N, 26°09'E, 12 May 1960, R & N /3764. o o E. Aegean islands. Ikaria, Onnos Praia. 37 34'N, 26 09'E, 17 Jun 1958, R & S o o 1/327; Samos, Ag. Kiriaki, near !he W. shore ofthe island. 37 42 'N, 26 39'E. 27 May 1962, R & Sseeds, cult. R-4466. o o Kiklades. Folegandros, Ihe S.E. point, 36 36'N, 24 57'E, 23 May 1960, R & N Flora Mediterranea IO - 2000 399

14634; lraklia (S. ofNaxos), S. of lne harbour, 36°50 ' N, 25 °29'E, 7 Jun 1957, R o o 4233; Mikonos, N.N.W. ofthe town, 37 27'N,25 20 'E, 1 I May 1968, R & E 35042: Naxos, I km E.S.E. of Akr. Axapsis, 37°08'N, 25°25'E, 18 May 1957. R 3128: o o Sifnos. Ormos Kondos, 36 54'N, 24 42'E, 13 May 1958, R & S 8493; Skinousa (S. o o ofNaxos).36 52'N, 25 31'E, 9 Jun 1957, R seeds, CIIII. R·9 .

RanunclIlaceae

11 74. Clematis cirrI/osa L. - 2n == 16.

o o Gr: Kiklades. Naxos. 2 km E.N.E. ofKomiaki. 37 09'N, 25 32'E, 26Apr 1957, R 170./.

11 75. Delpllinium peregrinum L. - 2/1 '"' 16.

o o Gr : Kiklades. Naxos. the chapel N. of Oros Zeus, 37 02' N. 25 30'E, 4 Aug 1958, R & o o S seeds, clilt. R-1483; Siros, W. of Finikas, 37 24'N. 24 53 'E. 29 May 1968. R & Bo 33 783.

11 76. Delpllinium ,IIi tapllysagria L. - 2n = 18.

Gr: Kiklades. Naxos, 2 km S.S.w. of Filati, 400 m, 37°02' N, 25°29' E, 8 Jun 1957, R seeds,culi. R-168.

11 77. Ranunculus bulbosus L. subsp. neapolitanus (Ten.) H. Lindb. - 2" = 16.

o o Gr: Evvia.3 km W.S.W. of Akr. Kafirefs, 38 OS'N, 24 33 'E, 22 Jun 1958, R & S 11695: N. of Ag. Dimilriou. 3S008 ' N, 24°27' E, 23 Jun 1958, R & S 1/8/0. Kiklades. Naxos, the W. peak ofKoronos Oros, 900-1000 m, 37°08' N, 24°29'E, 2 Jun 1958, R&Sl0012.

11 78. Ranullculus cllius DC. - 2n = 14.

G r: Kiklades, los, N.N.W. ofthe highcst peak, 36°44'N. 25°20'E, 7 May 1957, R 2554: Naxos, 3 km E.N.E. of Mitria (Engares), 37°08'N, 25°28' E, 13 Ma)' 1957, R 2569.

11 79. Ranunculus creticus L. - 2/1 "" 16,

Gr: Kiklades. Naxos, 2 km E. of Apiranthos, 400 m, 37°04'N, 25°32'E, 2 May 1957. R o o 2055; Naxos. the chapel N. ofOros Zeus, 600 m. 37 02'N, 25 30'E, 14 May 1957, R seeds, culi. R· 1489, 400 Kamari, Felber & Garbari: Mediterranean chromosomc numbcr rcpoTtS - IO

1180. Ranunculusflcaria L. subsp. chrysocephala P. D. Sell - 2n = 32.

Gr: Kiklades. Kithnos, Driopis. 37°23'N, 24 "'26' E, 12 Apr 1969, R. SI & G 40569.

1181. Ranunculus marginatus Dum.Urv. - 2n = 16.

o o Tu: Mugla. GOcik, N. ofthe town, 36 42'N, 29 06'E, 5 Jun 1967. R & Be 29506.

1182. Ranunculus murlcalus L. - 2" = 32.

Gr: E. Aegean islands. Ikaria, S. ofEvthclo, 37°38'N, 26"' 11 ' E. 24 Apr 1958. R & S 6713. o o Kiklades. Naxos, the beach 3 km N. of Mitria (Engarcs). 37 08'N. 25 26'E, 18 May 1957, R 3090; Naxos, S. ofKato Potamia. 37"' 05'N, 25 °26'E, 29 May 1957, R 3467.

1182a. Ranunculus murlcalus L. - 2n = 48.

Gr: Kiklades. los, N.W. of mI. Prof. El ias, 200 m, 36°44'N, 25°20'E, 6 May 1957, R 2477; Naxos, 3 km S. of Akr. Axapsis, 37°07'N, 25 "' 25' E. 30 Apr 1957, R 193/; Naxos, Axapsis IO Mitria (Engares), 3?"'07'N. 25°26' E. 3 Jun 1957. R 3838: Naxos, o o 2 km E. of Apiranthos, 500 m, 37 04' N, 25 32' E, 2 May 1957, R 2/33.

The ripe achcncs ( includins the beak) wcre 6-8 mm long in the hexaploids, while lhose of the letraploids were 4-6 mm.

1183. Rununculus sprunerlanus Boiss. - 2" = 16.

o o Gr: Evvia.3 km W.S.W. of Akr. Kafirevs, 38 08 ·N. 24 33'E. 22 Jun 1958, R & S 1/695. Kik ladcs. lraklia (S. of Naxos), 1 km W. of Onnos Pegad i, 36°50'N. 2So28'E. IO o Apr 1958, R & S 5423; Naxos, Koronos Oros, the W. peak, 900- 1000 m. 37 08'N. o . o 25 31'E, 30 May 1958, R & S 9727; Paros, MI Prof. Ehas, 650 m, 37 Ol'N. 15° 12 ' E, 18 Jul 1958, R & S seeds, cult. R- 1377: Sifnos, Livadi, 36"' 56'N. 24 "'4 1' E, 9 Apr 1969, R, SI & G 4032 7.

1184. Ranunculus Ihas iu5 Halaesy - 2n = 16.

Gr: E. Aegean islands. Ikaria. MI Atheras. 800 m. 37°38' N. 26° 11'E, Il Jul 1958. R & S seeds, culI. R-1261. Endemie IO the N. and C. Aegean areas. To my knowledge. Ùl e first report for Ihis species. Flora Mediterranea IO- 2000 401

- ...... t.~ ttl.,,~ .;:" :'- .~ ... ft..... b

Fig. l. A photomicrogmph and a drawing or somatic metaphast: platt: of: Il, Geli/sIa 1/I0IlSpeSSU­ lana, 2n = 48; b. Genisla corsica, 20 " 48. - Scale bars" 5 !lID.

Resedaceae

1185, Reseda lulea L. subsp. lulea - 2n = 48.

o o Gr: Kiklades. Skinousa(5. ofNaxos), 36 52'N,25 3 1'E, 9 Jun 1957, R 4329.

Saxifragaceae

1186. Saxifraga graeeu Boiss. - 2n = c. 50.

G r: Kiklades. Naxos, E. of Stavros Keramotis, 650 m, 37°06'N, 25°32'E, 4 Apr 1957, o o R 750; Naxos, 2 km S. of Moni, 400 m, 37 04'N, 25 30'E, 1 Apr 1957, R 624; Naxos, Oros Zeus, 650 m, 37°03'N, 25°30'E, 23 Mar 1957, R 336.

The chromosome number n = 32 has been reported by Larsen & Laegaard ( 1971) from Sicily for S. cf. graeca, with the reservation that the leafshape differs from Greek materi­ al available in Copenhagen (C). According to Pignatti (1982, p. 515) S. graeca does nOI 402 Kamari, Felber & Garbari: Mediterranean chromosome num ber reports - IO

occur in Sicily but instead the closely related, W. Mediterranean S. carpe/ana Boiss. & Re uter. The coulIl by Larsell and Laegaard has apparently by mistake been referred to S. graeco (as S. carpe/ana subsp. graeca) in Jalas et al. ( 1999), although the map (no. 3173) shows that S. graeca does not occur iII Sicily. Therefore the present counts are apparently the first ones for S. graeca.

11 87, Saxifraga hederllcea L. - 2n '"" 36.

Gr: Kiklades. Kea, 1-3 km S. ofChora, 300 m, 37°38' N, 24°20' E, 16 Apr 1969. R. St & . .. G 40755; Ktthnos, Kastro, 37 28'N. 24 24 ' E, 12 Apr 1969, R, St & G 40534.

To my knowledge this is the first chromosome repot1s for the spccies.

1188, Suxifraga triducty/i/es L. - 211 '" 22.

Gr: Kiklades. Kea, 1-3 km S. of Chora, 37 •38'N , 24 •20'E , 16 Apr 1969, R. SI & G 40754.

Rderencts

Aryavand A. t957: COlltributioll Il l'èlude cytotaxinomique de quclques Crueif,,!res de l'Iran et dc la Turquic. - Bull. Soc. Neuchmel. Sci. Nat. 98: 45, 54. Jllias J. & Suominen J. (eds.) 1989: Alias Florae Europacac 8. - Hel sinki. 261 pp. -& - 199 1: Alias Florac Europaeae 9. - l-Ii!l sinki. 11 0 pp. - & - 1994: Atlas Florae Europaealol IO, - ['Iclsinki. 223 pp. -. - & Lampi ll en R. 1996: Alias Flome Europaeae I t. - Helsinki. 3 10 pp. - . - . - & Kunto A. 1999: Ali as Flome Europacae 12. - I-k ls ink i. 250 pp. Larscn K. & Laegaard S. 197 1: Chromosomc number:s ofthe Sicilian flora. - Bot, Tidsskrifi 66: 252.253. Pignani S. 1982: Flom d'Italia. \'01 . primo. p. 51 4. 515. - Bologna. Svensson. S. 1984: Chromosome numbcr:s and morphology in thc Capsella buna-pastoris complcx (Bra.u icace~) in Greece. - Willdellovia 13: 267-276.

Adress of the author: Prof. Hans Runemark. DcpartIllcnt of Systcmalic Ilotany. Universit}' of LUlld. O. Vallgalan 20. S-22361. Lund, Sweden.

Repons (1189-1190) by Tiziana C usma Velari, Laura Feoli Chiapclla & Cianluigi Bacchetta

11 89. Genista monspessu/unQ (L.) L. John son (= Te/ùle monspesslllolla (L.) C. Koch)J - 2n = 46, 48 (Fig. la).

Sa: Mesu e Monte, Romana (Sassari). oligo-miocenic rhyolite, 400 29'N, 8°36' E. 180 m, 6 Dct 1997, G. Baccheua (CAG), s.n , . Lu: Estrada de Loures (Pontes), 38°49'N, 9° IO ' W, Jul 1989, sccds obtained from Botanical Garden, Lisboa (s.n. , s. coll., s. exsicc.). Flora Mediterranea lO - 2000 403

GeniSla monspessulana is most frequent in the centrai Western pan of the Mediterranean region and less frequent in the Easlem part; il is presenl also in Ponugal and in the Açores. 11 grows in the "maquis" and open Quercus i1ex woods (Gibbs 1968. Gibbs & Dingwall 1971. Pignaui 1982. Greuler & al. 1989). Our chromosome counl was 2n - 46, 48. Chromosome size ranges between 0.67 and 2.85 ).lrn . De Castro (1949) eounted 2n = ± 46 and 2n = 48. respectivel y sub ()·tisus 11/011- spessulanus L. and C. candicans (L.) Lam., in cultivated material from the Botanical Garden of Coimbra. Sai\udo ( 1973) reponed /I = 23 in populalions from Los Barrios (CAdiz., Spain). On the olher hand, Femandes & Santos (1975) found 2n - 48 in popula­ tions from Pono, Lordalo do Ouro (Ponugal). Some aulhors (Gibbs 1966, 1968, Gibbs & Dingwall 1971, Pignaui 1982, del Arco Aguilar, 1983) prcfer IO mainlain the indi vidualilY of lhe genus reline Mcdie. for the co­ presence of "Genisla-characlers" Cupper lip of the calyx deeply bifid, keel oblong, leaf wilh three vascular traces al the node) and of"Cytisus-characters" (Ieaves trifoliolate, usu­ all y petiolate, seeds strophio1ale). Others (Polhill 1976, Oreuter & al. 1989) have choscn , •

,

a

•~• I , ) ... • -• ..: '... --, . , .,... '.. , , .. ,",, , '. -r . • • r ,", b

Fig.. l. A photomicrograph and a drawin g or somatic mctaphasl! platI! or: a. Ge'llsta cephalalllllfl subsp. cephalantha. 2n - 26 + 20; b. Genisla rl'icuspidata, 2n = 48 + 29. - Arrows indicale B-chro­ mosomes. Scale bars " S I!m. 404 Kamari, Felber & Garbari: Mediterranean chromosome number repons - lO

IO include it in Genisla L. The centre of disu ibUlion of this group is in the Canary Islands, some species bcing presenl also in the Madera and Açores Islands, and in Ihc Medilerranean region, particu­ larly in the Western parto Genisfa monspessulona is Ihe mosi widely dislributed species, the only other species presenl in Europe, according IO Gibbs ( 1968) and G ibbs & Dingwall (1971), bcing Genista lini/olia L. (=Teliflc linifolia (L.) Webb el Berth.J. Talavera & Gibbs (1999) have rcccntly added IO Ihe genus Teline also r palens (DC) Talavera & P.E. Gibbs and T. fribracleolala (Wcbb) Talavera & P.E. Gibbs, which are endemie to Eastem and Southem Spaln respeclively and wcrc prcvlously placed by Frodin & Heywood (1968) and Polhill (1976) in Cylisus Desf.

1190. Gellisla corsica (Loisel.) DC. - 2n = 48 (Fig. I b).

Sa: Pranedda, Pula (Cagliari), granite, 39°2'N, 8°50'E, 760 m, I lui 1998, G. Bacchetta (CAG), s.n. Sassari. Baldedda, 400 44'N, 8°34'E, 225 m, 12 Jul 1989, S. Diana Corrias. s. n.

Genisla corsica is endemic to Corsica and Sardinia, where il is widely distributed al varo ious altirudes (Gibbs 1966, Valsecchi 1977). The chromosome number 2n = 48, based on 30 mClaphase plales, confirrns existing ref· erences. In fact, Conlandriopoulos (1957, 1962) reports 2n = 48 far plants originated from the " Fon génois, Col de Vizzavona", from the "Défilé de l'Inzecca'' and from Zonza (Corse); Villa (1978) coumed thc same somatic number on samples from Cala Gonone, Codula di Sisine (Sardinia). Chromosome size ranges between 0.77 and 2.42 flm. The speeies belongs IO secl. Scorpioides Spach. The chromosome number 2n = 48 is by far the most frequent fo r the species o fthis section: Genistaferar; Poirct (Tschcchow 1931, Villa 1980), G. morisii Colla (Villa & Sanna 1983), G. cadasonensis Valsecchi (Vi lla 1988) e G. ifniensis A. Caballero (Cusma Velari & al. 1999).

Acknowledaements

The financial suppon by the Ministero dcII' Università e della Ricerca Scientifica e Tecnologica (Roma) is gratdul1 y acknowledged. We thank l'ror. Silvana Diana Corrias far having supplied us with the sceds.

Reference!

Arco Aguilar. M. del. 191:13 : Catalogo actualil.lldo de 105 taxones del genero Telille Medie. cn la region Maearonesica. Adiciones y nucvas eombinacioncs. - Vicraea 12 (1·2): 193-232. Castro. D. dc, 1949: No\'as numc:ros dc cromosomas para o géncro Cylisus L. - Agron. Lusil. Il (2): 85-89. Contandriopoulos, J. 1957: Contribution à l'étudc caryologiquc des endémiques de la Corse. ? Ann. Far. Sci. Marseille 26: 51- 65. - 1962: Recherches sur la flore endémique de la Corse et sur ses origines. - Ann. Fac. Sci. Marseille 32: 1-354. Cusma Velari, T., Feoli Chiapella. L~ Cristin, C. & Kosovc:l, v. 1999 : Karyological systc:m8lics of Gellisla ijlliellsis A. Caballero, Gellisla tricuspidata Dcsr., and related specics (Gcllisleae Flora Mediterranea lO - 2000 405

-Fabaceae). - SlUd. Geobot. 17: 77-8]. Femandes, A. & Santos, M.F. 1975: Contribution à la connaissancc c)1otaxinomiquc des SpermmophY/a du Portuga1. IV. Leguminosae (Suppl. 1l. - BaI. Soc. Brot. 49: 17]-196. Frodin. D.G. & Ileywood, V.ll 1968: C},tislis L. In: Tutin, T.G., Heywood. VJ1., Burges. N.A .. Moore. D.M., Valcntinc. D.H .. Waltcrs. S.M. & Wcbb. D.A. (cd.). Flora Europaca. 2: 86-90. - Cambridge, Gibbs, P.E. 1966: A rcvision ofthe genus GenislQ L. - NoI. Roy. Bot. Ga rd. Edin b. 27(1): 11-99. - 1968: 7èline Medie .. In: Tutin. T.G., Heywood. V.H.. Burgcs, N.A., Moore. D.M .. Valenti ne. D.H .. Walters. S.M. & Webb. DA (ed.l. Flora Europaea. 2: 9]-94. - Cambridge. - & Dingwall, I. 1971: A rcvision ofthe genus Te/ine. - BaI. Soc. Bro!. 45: 269-]16. Greuter, W.• Burdet, H.M, & Long. O. (ed.) 1989: Mcd-chccklist. 4. - Genèvc. Pignatti, S. 1982 : Fl ora d·ltalia. 2. - Bologna. Polhill, R.M. 1976: Genisleae (Adans.l Benth. and rclaled tribes (Legumillosae). - BaI. Sysl. l ' 143-368. Sanudo, A. 1973: Variabilidad cromos6mica dc las Genisteas dc la Flora espanola en rdaci6n con su ecologia. 1. Numero y comportamiento de los cromosomas durante la me iosis. C. Seccion Cephalosparlum dci Oen. Genisla L. y géneros Lygos Adanson. Spartium L.. Teline Medic .. Calicolome Link y Argyrolobium Ecklon & Zeyhcr. - Cuad, C. (1 iol. Univo Granada 2(2): 117- 120. Talavera, S. & Gibbs. P.E. 1999: Teline Medik. - Pp. 14J · 147 in: Talavera. S.. Aedo. c.. Castrovicjo. S., Rom cro larco. c.. Saez.. L Salgueiro. F.L & Velayos. M. (ed.), Flora Iberica. 7 (1). ­ Madrid. Tschechow. W. 1931: Karyologisch systematischc Untersuchung der Triblls SopllOrY!oe. Podo~vrieoe und Genisteae. -Izv. Tomsk. Old. Gosud. Russk. Bot. Obsc. 3: 121- 131. Valsecchi, F. 1977: Le piante endemiche della Sardegna: ]8. - BolI. Soc. Sarda Sci. Na!. 17: 318- 323. Villa, R. 1978: Numeri cromosomici per la Flora Italiana: 457-463. - Inform. Bol. Ital. l O: 241-243. - 1980: Numeri cromosomici per la Flora Ital iana: 733-741. - Infonn. Bot. haI. 12: 155-160. - 1':I1$1S: Numeri cromosomici per la flora Italiana: I 197· 1204. -Inform. 130\. Ital. lO: 047-M2. -& Sanna, D. 1983 : Numeri cromosomici per la Flora Italiana: 956·961. -Infonn, Bot. haI. 15: 49-52.

Address of the authors: Tiziana Cusma Velari & Laura Feoli Chiapclla. Dipartimento di Biologia. Università degli Studi di Trieste, Via Licio Giorgi eri 10,1·34127 Trieste, Ital)', Gianluigi l3acchella. Dipartimento di Scienze Botaniche. Università degli Studi di Cagliari. V.le S. Ignazio da Laconi Il 1-09123 Cagliari. haly.

Reports (1191 -11 92) by Tiziana Cusma Velari, La ura Feoli Chiapella & Ve ra Kosovel

119 1. Genista cephalantlla Spach subsp. cephafanllla - 2n = 26 + 28 (Fig. la).

Ma: Nador-Melilla, clearing in a Mediterranean wood, 35°16'N, 2°58'W, 200 m, 25 Jul 1989, L. Feoli Chiapella et E. Feoli (TS8), s.n.

Genista cephalantha Spach subsp. cephalantha grows on the coastal ranges of Marocco and Western Algeria (Quezel & Santa 1962, Raynaud 1979, Maire 1987). A chromosome number of2n = 26 + 28 = 28, based on 7 metaphase plates, was found; 406 Kamari, Felber & Garbari: Mediterranean chromosome number reports - IO

chomosome size ranges between 0.66 and 2.64 ~m . 0.33 - 0.44 ~m for B chromosomes. There are no referenccs IO our knowledge regarding Ihe caryologieal data of this taxon . This number may be considered a case of hyperaneuploidy (ascending aneuploidy). the basic number heing x= 12. Thcre are no reports of 2n = 26 for taxa of Genista. Very few cases have been found of n = 26, 2n = 52, such as in G. oemensis (Biv.) DC, in popula­ lions of both Sardinia and Sicily (Forissier 1973. Villa 1988, Cusma Velari & al. 199 7). Genisra cephalantha Spach subsp. cephalamha belongs IO sect. Cephalosptlrfum Spach. The section has a Westem Mediterranean distribution. 1t includes six species limit­ ed IO Northem Africa. from Morocco to Libya (Greuter & al. 1989): G. capilellaw Cosson. G. cephalamha Spach, G. clavata Poiret. G. demnatensis Murb. [= G. cephalantho subsp. demnatensis (Murb.) C. Raynaud}, G. microcephala Cosson & Duricu, G. quadrijlora Munby and G. umbellata, whieh is tbe on ly species present in Europc (Gibbs 1966). The latler is, so far, the on ly karyologically slu ~ied laxon wilhin the seclion. The chromosome numbers n = 23, 2n = 46 were counled by Sai'ludo (1973) and Cusma Ve lari & al. (1998), 2n = ± 42 by Santos (194445).

11 92. Genlsta tricuspidala Desf. (= Genisw lucida Camb., G. tricuspidaw varo lucida (Camb.) O. Bolòs & Molin .l ~ 2n = 48 + (O-4B) (Fig. I b).

BI: Mallorca, betwecn Andratx and SanI Telm. clearing in a Pine wood, 39°33' N. 2°22' E. 100 m, 2 Aug 1999, L. Feoli Chiapella et E. Feoli (TSB). s. n. Mallorca, NE- KUste. Cabo Capdepera. 39°41 'N, ) 026'E. Royl. seeds oblained from Botanical Garden. Berlin-Dahlem (s. n., S. exsicc.).

Genista lucida was considered as endemie lo tbe island of Mallorca by Kn ochc (1922). Gibbs (1966), Bonafè BarcelO (1978). Peris & Stubing (19lS5) indicate tha! the species also occurred in Sautheaslem continental Spain (Cabo Moraira, Alicante). Recenti)' Talavera (1999) eSlablished tbc idenlity ofG. lucida witb G. tricuspidata, already suggesled by Gibbs (1966) and Maire (1987) as endemie to Northweslem AtTica (Morocco. Algeria and Tun isia). A chromosomc num ber of 2n = 48 + (0-4B), based on 22 metaphasc plates, was counl­ ed; chromosome size ranges betwecn 0.88 and 2.09 ~m , 0.44 lIffi for B chromosolllcs. These are tbe first chromosome data oblaincd from malerial deriving from the European populalions ofthe species; Cusma Velari & al. (1999) have counled 2n = 48 for Moroccan populations (Beni-Snassen. Imi-n-I fri). Genista tricuspidata belongs to secl. Vog/era (Gaenn., May. & Schreb.) Spach. Thc chromosome number 2n = 48 was reponed in several taxa ofthis seelion: G. hirsuta Vahl (Sai'ludo 1972, Gallego Manin & al. 1986), G. aristalO C PresI (Cusma Velari & Feoli Chiapella 1991). G. cllpanii Guss. (Banolo & al. 1977), G. anatolica Boiss. (Krusheva 1975). G. carina/is Griseb. (Kuzm anov 1975) and G. germanica L. [Reese 1952, Semerenko & Shvel 1989 (after Goldblatt & Johnson 1991»).

Ack nowledgemf nts

The financial support by the Ministero dcII' Univcrsità e dclla Ricerca Scientifica e T«nologiea (Roma) is graterully aeknowledgcd. Flora Mediterranea lO - 2000 407

~ a , b , , J , .. ~ ,1 "" .so • . - , • • , , , .. , • • • , ~ ~ , 'ti' 'It • , • ~ . , • \ -:-, , e -- "T' e • .. .. • . ~ r • • r. ~ . " •• • i • • • ~ • ,". !. • • , I \/~--:"~ ~ • ·1" '- " /'

Fig. 1. Methaphase plah::s of: a. AnchllS(I crispa subsp. crispa. 2/1 = 16: b. A/lchusa procera . 211 = 16; c. Anchusa pusilla , 2" =32. - Scale bars '" IO /lm. 408 Kamari, Felber & Garoari: Mediterranean chromosome number reports - IO

a • • JI· ! • -. I

• • • \ • • • • • • c I -- l l - ) . - • ,.- ". . -. •• ..J,/., t; I , ...... • d .. ,., - -'\ ... •r \ l , •. ~ . \ ~ "- .... ' , , '1'.\ , ~ , ... O. ,) '..s- ·- . .,. V , ,f ~ .. Il . .' .. .t ., \ '00 •• • .'. , ....1 S ' \ • ... .. - . Fig. 2. Mcthaphasc platcs of: I , Ilnchl/sa Ihessala. 2n = 12: b. AncJmsu 1llldllla/a subsp. hyb,.ida. 2n = 16; c. Anchusa capellii, 2/1 = 16: d, PulmO/laria rubra. 2n = 28; t , Lycopsis oriemalis. 2n " 16. ­ Scale bar = lO 11m. Flora Med iterranea lO - 2000 409

Il Il Il .... Il l. Ii Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il

~I, __~ __~'~' I~I __~ ____~I J 101m .... " 101 m .... " a b

Il Il Il Il Il Il Il Il .1 Il Il Il l. Il Il l. Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il

• m " c Il Il Il Il Il Il Il Il Il Il Il Il Il .1 Il Il Il Il Il Il Il Il Il Il Il Il Il Il I I I I , I I , I m .... ., M m .m ., d e

Il Il Il Il Il Il Il .. Il Il Il Il Il Il Il i l Un 11 11 ,11111111 ~IIIIIII , 111111 ,II M m sm st m s m st f g

Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il Il

m .m h

Fig, 3. IdiogrJms of: a. AI/ciII/sa C l'i~pll subsp. cl'i.l]m; h. Alldw.wl prm:era: c. AII('hu~' a plI.\'ilfa; d, Ancill/sillh('~'.m/a: e, Al/ehUl'a IIndlllU/o subsp. hyhridu: f. AllcJlIISiI (;apdlii; Il , Lycopsis orielllalis: h. PII/mOllar;a /'IIhra. 410 Kamari, Felber & Garbari: Mediterranean chromosome number rcpons - IO

Rt(t rences l3 al'lolo. G., Brullo. S .• Majorana. G. & Pavone, P. 1977: Numeri cromosomici per la Flora Ilaliana: ]05-]28. - Infonn. Bot. Ilal. 9: 7 1-87. BonaR l3arcel6. F. 1978: Flora de Mallo~n. 2. - Mallorca. Cusma Velari. T. & Fecli Chiapclla. L. 1991: Syslcmatic relationships within the GeniSI(J sylnmris group (Gem"steae. Fabaceae) on thc basis of karyological and biometrical data. - Flora Medit. I : 2 1- 29. -, - & Mangiavacchi. L. 1997: Rcports (840-842). [In Kamari, G .. Fclbcr. F. & Garbari. F. (ed.). Mediterranean chromosome numbcr Ti:ports · 7j. - Flora Medil. 7: 2]6-240. - , - & Cristin. C. 1998: Reports (994.995). [In Kamari. G .. Felbcr. F. & Garb.1ri. F. (ed.). Mediterrancan chromosome numlKr rcpons· 8]. - Fl ora Mcdit. 8: 291-29]. -. -. - & Koso\'cl. V. 1999: Karyological systematics of GenisTa ijniensis A. Caballero. Genisia In'cuspidata Desf.. and related species (Genisleae -Fabaceof). - Stud. Gcobot. 17: 77-8]. Forissier. R. ]97]: Rcports. [In LOvc. A. (ed.). ]01>13 chromosomc numbcr repons XLI II . - Taxon 22(516): 647-654. Gallego Manin. F.. Sanchcz Anla. M.A. & Na\'arro Andrés. F. 19M: Acerca de la cariologia dc algu. lIas gellistcas del cent(()-()(;cidente cspanol. - Lazaroa 9: 55-60. G ibbs. P. E. 1966: A rcvision ofthc genus Genisla L. -Not. Roy. Bot. Gard. Edinb. 27( 1): 11 -99. Goldblan. P. & Johnson. D.E. (ed.) 1991: Index to pianI ehromosomc numbers 1988-1989. Mi ssoori Bot. Garden. Greuter. W., Burdet. H. M. & Long. G. (ed.) 1989: Mcd·chccklist. 4. - Gcnèvc:. Knoche. H. 1922: Flom baleari ca, 2. - Mompellicr. Krushe\'a. R. M. 1975: Repor1s. [In U~vc. A. (cd.). IOPll Chromosomc Numbcr Reports LI . Taxon 24: 6 71 -678. Kuzmanov. B. A. 1975: Reports. [In Uh'e. A. (ed.). lOPS Chromosornc Numbc:r Reports XLlX].­ Taxon 24: 501-5 16. Main::. R. 1987: f'lore dc l'Afrique du Nord. 16. - Paris. l'cri$. J. 11 . & StObing, G. 1985: Gellista IlIcid(. Camb. eli Cabo Moraira (Alicante). - Lazaroa 6: 295. Qw:zel. P. & Santn. S. 1962: Nou\'clle Flore dc l'AIgéric: et dcs régions llé sc:niques méridionulcs. I. - Paris. Rayllaud. C. 1979: Le genre Genista L. au Maroc: Monogmphie. iconographic. cles dc delennina· lion. ? Naturnlia Monspcliensia 28: 1-52. Reese. G. 1952: Erg!lnzendc Mineilungen OlKr die Chromosomenzahlen millclcuropaischer Geràsspnanzen. I. - Ber. Deutsch. Bol. Ges. 64: 240-255. Santos dosoA. C. 1944-45: A!gumas contagens de Cromosomas nos Géncros Ge/lis/a L. c C)'lisIiS L. ~ Bol. Soc. I]rol. 19: 519-522. Sanudo. A. 1972: Variabilidad cromosomica dc las Gcnistc:as dc: la Flora csp:mola eli n:hlciòn con su ecologia. lo Numero y componamicnco dc: los cromosomas durantc la mciosis. B. Secciones Genista. Span ioides Spach. Plryffospanium Willk. y l'aglera (Il. Gac:nner. Il Mcycr & Schreb.) Spach. del Geli. Genisla L. - Cuad. C. Biol. Univo Onmada 2: 4] - 52. - 1973: Variabilidad cromos6mica dc las Gcnisteas dc la Flora c:spanola CII rc lacion con su ccologia_ l. Numero y componamicnto dc los cromosomas durante la IIIciosis. C. Seccion Cephalospar/um del Oen. Genisla L. y géneros l.ygos Adanson. SparliUIII L .. Telille Medic .. Calicolome Link y i1rgyrolobiulII Eckloll & leyhcr. - Cuad. C. Biol. Univo Granada 2(2) : 117- 120. Talavera. S. 1999: Genista L. - Pp. 45-119 in : Talavem, S .• Aedo. C .. Castroviejo. S .. Rornero Za~o. C .. Sàez. L.. Salgueiro.l-". l . & Vclayo$. M. (ed.), Flora Iberica. 7( 1). - Madrid. Villa. R. 1988: Numeri cromosomici per la Floro lt ali an a:1 1 97-1 2~ . - Infonn. Bot. 11 01. 20: 647-652. Flora Mediterranea 10- 2000 4 11

Address of the authors: Tiziana Cusma Velan. Laura Feoli Chiapel1a & Vera Kosovel. Dipartimento di E1iologia. Università degli Studi di Trit.'Sle. Via Licio Giorgicri IO. [.)4127 Tricslc, haly.

Reports (1193. 1200) by Massimo Bigazzi, Graziana fio rini & Federico Selvi

1193, A nchusa crispa Vivo subsp. crispa - 2n = 16 (Figs. la, 3a).

Il: Sardinia, Gallura (S5), retrodunal areas of the bcach " La Marinedda", Isola Rossa. 41 0 1' N, 8"53'E, 4 Jul 1998, Se/vi 98.001 (FI).

Karyotype fonnula: 2n = 2x "" 2M + 6m + 6sm + 2sl·SAT = 16. This number matches previous findings on ssp. crispa from Sardinia and Corsica (Contandrioupolos 1962), and on ssp. maritùna from Sardinia (Valsecc hi 1976, SeIv i & Bigazzi 1998). However, thete are some differences in the chromosome morphology of lhis populat ion in respect ofthe othcr examined provenances oflhe Iwo above·memioned subspecies. Th is karyotype poly­ morphism is consislent wilh the high degree of morphological varialion shown by this Cymo-Sardinian psammophylic endemie, which is slill in aCl ive evolution.

1194. A lle/.usa proura Besser ex Link - 2n = 16 (Figs. Ib, 3b).

Gr: Evros, Nom. Oresliadas, sandy fie lds along the ri ver Ardas close IO Rizia, ca. 80 m, 4 1"36'N, 26°22'E, 24 Ju n 1999, 8iga=zi & Selvi 99.0/4 (FI).

Karyotype fonnula: 2n = 2x = 2M + 6m + 6sm + 2s1 -SAT - 16. A. pr()cera belongs to the groupof A. ojJìcinalis L. (2/1 = 16), and is mainly distribuled in SE Europe. This counl confinns a previous repo rt from NE Gl'cece (Slrid 1980).

1195. Anchuj'Q pusilla Gu"u l. - 2n - 32 (Figs. le, 3e).

Tu: BS Nevsehir, fa llow fie lds around OrgOp, ca. 1100 m, 38°42'N. 35°56'E. 4 Jun 1997 , Bigazzi & Se/vi 97.0/8 (FI).

Karyotype formula: 2n = 4x · 8M + 10m + IOsm + 4s1 =32. This is thc firs l report fo r Il . pusil/a, an annual species stretching from Eu ropean Turk ey to centraI Anatol ia. A. pusilla is relatcd to thc perennial A. azurea M iII. (A. italica Relz.), a widcspread mediterranean species wilh a tctraploid complemcllt of 2n - 32 chromosomes (D'Amato & Trojani 1985). The complement of A. pusilladiffers from thal of A. azurea in Ihc absence of$AT· chromosomcs.

1196. A nchusa /hessala Boiss. & Spruner - 2n = 12 (Figs. 2a, 3d).

1ù: A l Edime, ruderal plaees along the road between Ulleburgaz and Babaeski. sandy soil, ca. 40 m, 41 "25 ' N, 27°IS'E, II Jun 1997, Biga::!' & Se/vi 97.012 (FI). Karyorypc fo rmu la: 2n = 2x = 2m + 8sm + 2st ·SAT = 12. This counl confinns previ· 412 Kamari, Felber & Garbari: Mediterranean chromosome number reports - IO

ous reports From Bulgaria (Markova 1983, Markova & Goranova 1995). However, tetraploid and hexaploid cytolypes wim 2n '" 24 and 36 have been reported. respectivel y, in planls From Romania (Tarnavschi 1948) and Bulgaria (Markova 1983). Alllhese find­ ings confinn me base chromosome number x = 6. which is unique within the genus Anchusa s.l. and the lowest one in the Boraginaceae (Brinon 1951).

11 97. Am:husa undu lata L. subsp. hybrida (Ten.) Bég. - 2n = 16 (Figs, 2b, 3e).

Tu: 8 5 Vozgat, ruderal places along the road to Sorgun, ca. 11 50 m, 39"II'N, 35"6'E, 4 Jun 1997. Biga:zi & Se/vi 97.010 (FI).

Karyotype fonnula: 2n ;. 2T = 2M + 6m + 6sm + 2 st-SAT '" 16. Thi s report is the first one for Turkey and confirrns previous eounts from olher Mediterranean counlrics (Srnith 1932. Valsecchi 1976, Capineri & al. 1978. Strid 1991 , Markova & Goranova 1995. Selvi & Bi gazzi 1998). A high dcgree of karyorype slability oecurs in Ihis polymorphic and widespread Mediterranean laxon.

1198. Anchusa capellil Moris - 2n = 16 (Figs. 2e, 3f),

Il: Sardinia, Esterzili (NU), rock fissures and slOny pasturcs on thc summit of Monte Santa Vittoria, ca. 1200 m, 39"44'N, 9"18'E, 30 May 1999, BaccheI/a & Se/vi 99.002 (FI, CAG).

KaryOlype Fonnula: 211 ;;; 2"( '" 2M + 6m + 6sm + 2st-SAT '" 16. A previous eQUnt from the same locality, whieh is me only one known for Ihis endem ie species, is here confinned (Valsecchi 1976, Selvl 1998, Selvi & Bigazzi 1998). The complemenl here observed, howev­ er, dilfm from thc previous one in me presence of 2 M and 8 (ramerthan 6) sm ehromosomes. t 199. Pulmonarla rubra Scho" - 2n = 28 (Figs. 2d, 3h).

G r: E. Makedonia, DI'lima, Mt Rh odopi, montane woods near the vil1age or Prasinadas (Paranésti), ca. 1350 m, 4 1°13'N, 24°3 1' E, 26 Jun 1999, Biga::i & Se/vi 99.025 (FI, culta). Karyotype fonnula: 2n = 4x = 20m + 8sm = 28. This tetraploid eomplemcnt docs not mateh the diploid number 2n " 14 reported for Bulgarian and Greek provenanees. This lax­ onomieal1y criticai species mainly oecurring in S.E. Europc (Markova & Ivanova 1970; Strid & Franzén 1983).

1200, Lycopsis orlenla/is L. - 2n = 16 (Figs 2e, 3g).

Hcterorypic synonym of this spccics is Anchusa ovaIa Lehm.; homotypie synonyms are Anchusa arve1lsis (L.) M.Bieb. subsp. oriemalis (L.) Nordh., A, orientalis (L.) Rcichenb. fil. and Lycopsis arveluis L. subsp. orienla/is (L.) KuZJl,

Tu: A9 Kars. Fallow fields around the eastern shores of the Cildir GOl u. igneous Flora Mediterranea lO - 2000 4\3

1 • 2 .. • • ..... , ' -. . " , ~.

3 4

5 6 •. , ..."- 't. .,. ( .._.... ,- ro . • • •

Fig. 1·6. MilOlic rnctaphasc platcs or: I, Argyroc)'lislis ballalldieri. 211 = 50: 2, Chullla('cytfslIs mal· Iis, 2n = 50; 3, Cylislis jOltlane1;; subsp_ plumwus, 2/1 -.- 50: 4. Cylisus grandiflorus subsp. barbartlS. 2n = 46: 5, C)'llsus villosI/s. 2/1 = 52: 6. Gellisla manspessulana. 2n - 46. - Scale bars = lO ~m. 414 Kamari, Felber & Garbari: Mediterranean ehromosome num her repons - IO

soil, ca. IS50 m, 4 1°4'N, 4) OI9'E. 27 May 2000, Bigazzi & SelviOO. IO (FI ). Karyolypc fonnula: 2n '" 2l' == Sm + 6sm + 2sl·SAT '" 16. This eount eonfinns a previ· ous re port from eultivatcd plants ofunknown geographic origin (Strey 193 1). The diploid number of th is W. Asiatic speeies suppon s its separati on, al the spccies ra nk. from the related L. arvensis, ofwhieh was considered as subspeeies by several authors. L. ar\'ensis has a Central· European distribution and an hexaploid chromosome complement of 2n '" 6x = 48 (Gadctla & Kliphuis 1970, Bigazzi & al. 1997). In both taxa. karyolype morphol· ogy is ve!)' simi lar to mosl Anchllsa spedes.

Referen<:es

Bigazzi. M .. Nardi, E. & Selvi. F. 1997: .""chl/sella, a nt:w gc:nus of Boraginaccac from the CCnlral­ Easlc:m Mcditcrrancan. - PI. Sysl. E,·ol. 205: 241-264. Brinon. D. 195 1: Cytogcnctic studies on lhe Boraginaceae. - Ilrillonia 7: 2))·266. Capineri. R .. D'Amato. G. & Marchi. P. 1978: Nume ri cromosomici per la flora italiana n. 577. Inform. Bot. Ilal IO: 456. Contandriopoulos. l. 1962: Rccherches sur la flore endemiquc dc la Corse et sur SI.'S ongins. Ann. Fac. Sci. Marscille 32: 1-354. D'Amato, G. & Trojani. Z. 1985: Giemsa banding and \.:aryoty~ in IhTec sp.;:cies of AndlUs" (Boraginaceae). - Caryologia 38: 1]-2 1 . Gadella. T.W.G. & Kli phui s. E. 1970: CYlota.\onomic invesligalions in some angiospenns l,:Oll cClcd in the valley of Aosta amI in Ihe national par\.: "Gran Pamdiso·'. - Caryologia 23: ]6]·]79. Marko\'a. M. 198]: Iln LOve, A. (ed), 100lf>Chromosome Number Repons LXXVI III. - Ta\on 32: 140. - & Ivanova. P. 1970: Karyologische Untersuchungen dcr Vcnrcler der Film. Boraginaccac. Labilliac und Scrophula riaceae in Bulgarien. 1. - IlV. BuI. losi. (Sofia) 20: 93-98 . - & Goranova. V. 1995: Repons (4]5,47]) I[n Kamari, G .. Felbcr. E & Gerbari. F. led.). Meditemmean chromosomc numbt!r repons ·5). -1'1. McdiI. 5: 289· ]16. 5eh'i, F. 1998: Fiorai biometrics in the Anchus" IIndl/fala L group (lJoraginaceael from thc Centrai· Eastem Mcditerranean. - Bot. 1. Linn. Soc. 128 : 25 1-270. -& Bigazzi, M. 1998: Andlllsa L. and allicd gcnera (Boraginaecac) in Ital)'. - l'l. l3iosystcms 132: 110- 140. Smith. S.O .. 19]2: Cytology of Anclmsa and its rclation lo thc ta.\onomy ofthe gcnus. - l3ot. Gaz. 94 : ]94-40]. Stre)', M. 19] I: Kar)"ologische studien an Ilorraginoidcae. - Pianta 14: 68]-730. Strid, A. 1980: Repons [In !.Ave. A. (ed). IOIlP Chromosomc Number Repons no. LX IX]. Taxon 29: 709-7 10. - 1991: AIlc1lUsa L. In: Sirid, A. & Tan. K. (cd.). Mountain Flora ol' Gretce 2: 44-46. Edinburgh Universit)' Press. - & Franl.tn. R. 1983: Chromosomc numbers in flowcring ('IlanlS from Grcl'CC. - Wi lldenowia U : ] 29-]33. Tamavschi. I. T. 1948: Die chromosomenzahlen der Anthophyten flora \'on RUmanicn mi! einem Ausblic\.: auf das Polyploidie Problem. - Ilu ll. l ardo Bot. Univo Cluj. 28: 1-130. Valsccchi. F. 1976: Il genere Anehusa in Sardegna. - Webbia 30: 4]-68.

Address of lhc authOB: Massimo 8igazzi. Gra:i(iarn! Fiorini &. Federico Sc]v; Dipanimento di Biologia Vegetale. Via G. La l'ira 4 1-5012 1 Firenze. Italy: Tel.: + ]9 55 27562 15: Fax: +]9 55 2757]7]. Corrcsponding aut hor: F. Sclv!. E-mail: sel\[email protected] Flora Mediterranea IO - 2000 415

ReporlS (1201-1207) by Hikmat Tahiri & Paloma Cubas

1201. ArKYrocytisus baltulldieri (Mairc) C. Raynaud - 2n = 50 (Fig. l).

Ma: Moyen Atlas, sur la roule cnlre Azrou cl Timahdilc, 33 °20'N. 5°1 J 'W. 1800 m, 20 Scp 1998, Tahiri, RAB62 163. - (Fig. 1) Rif,jbel Tizirene, 35°01'N, 4°56' W, 1800 m, 14 Sep 1997, Tahiri, RAB62 169.

The monotypic genu$ Argyrocytisus is an endemie of Ihe Middle Alias and the Rif Mountains of Morocco. We havc counted 2n = 50 chromosomes in root mitosis of both populalions. These are Ihe firsl dala oblained from wild populations and agree wiih thc only previous report (Castro 1949) on plants growing al the Honus Bot. of Mooreslwon.

1202, Cllumuecylisus mollls (Cav.) Grcuter & Burdcl - 211 = 50 (Fig. 2).

M a: Maroc atlantique moyen, entre Bir Jdid et Souk el Tnine, 33 c20'N, 8c06' W. 17 May 1998, Tahiri, RAB62153.

Is an endemie species orlhc soulhern and centrai-western Morocco. The occurrence of Ihis spccies in the Canary Islands is doubtfu1 (Francisco-Ortega & al. 1992). No previous dala on the chromosome number of Ihis taxon have been reported. We counted 211 = 50 chromosomes in mitosis from root meristems.

1203. Cytlsus arboreus CDesf.) DC. subsp. baelicus (Webb) Ma ire - 211 = 50.

Ma: Rif, L8 Km avanl Bab Berred cn direelion d'lssaguène, 34c 5Y ' N, 4"46·W. 6 Jul 1998, Tahiri, RAB62168. Maroc atlantique moyen, forfl dc Benslimane, JJ"36'N, 7°C)7'W, 2 1 May 1998. Tahiri, RAB62133. C. arboreus is reslricted IO Morocco. Algeria, the lberian Peninsula and France. In Morocco, populalions growing in dif'ferent regions have been recogniscd as different sul>­ species: subsp. a,boreus (nonheast), subsp. catalallnicus (Rif, High and Middle Atlas) and subsp. baeticus (more widely dislribuled). Our previous dala from thcsc three subspeeies in Morocco, indicated 2n = 50 (Cubas & al. in press). We report dala from Iwo new popu­ lalions of subsp. baeticus, which also show 2n = 50 in root mitosis. These data diffcr from Ihe only prev ious report oflhis laxon (2n = 48, n = 24 bivalcnls) given by Sanudo 1973a. oblained from a Spanish samplc.

1204. Cytisusfon,anesii Bali subsp. plumosus (Boiss.) Nyman. - 2/1 = 50. (Fig. 3).

Ma: Moyen Atlas, enlre Imouzz.cr du Kandar el Ifrane. 33°48·N. 5°06' W, 1500 m, 27 Jul 1998, Tahiri, RAB62146.

C. fontanesii is restricled IO Morocco, Algeria and the south and east of Spain. In Morocco, dif'ferenl taxa have been classificd either al the vari elal (Maire 1987) or subspccif- 416 Kamari, Felber & Garbari: Mediterranean ehromosome numher repol1s - IO

ie rank based on the indument morphology. Cfontanesii subsp. plllmoslIs, which shows long patem hairs, grows in the Middle Alias and Rif Mountains. This is the firsl eount ofthc ehro­ mosome number ofthe this taxon . and agrees with previous studies (n = 25 bivalents) given by Safludo ( 1973b) carried out on plants from Spain wnich belong IO the subsp.folllanesii.

1205. Cylisus gra"diflorus (Sro!.) DC. subsp. barbarus (Jahandiez & Maire) Mairc 211 = 46 (Fig. 4).

Ma! Haut Atlas, jbel Oukaimcden, 31"13'N, 7"5I'W, 2250 m, 8 Ju1 1999, Talliri. RAB62 121.

C. grondiflorus is reslricted to Morocco .and the lberian Peninsula. In Morocco, two subspecies are recogniscd (Maire 1987): s ubsp. haplophyllu.t, an endemie of the Eastem Rif (jbel Gourougou) and subsp. barbarus , widespread in the nOl1hwest of Morocco. We counted 2n = 46 in root mitosis in a sample belonging lO subsp. barbarus. Our repol1 agrees with previous data for both subspecies from other Moroccan localities (Cubas & aL in press). However. these data diffcr from the reported chromosome numbers for susbp. grandiflorus in samples from Port ugal and Spain, which indicated differem chromosome numbers: 2n = 24, 2n = 48 or n = 24 (Castro 1949, Femandes & Santos 1971. Sai'! udo 1973a, Horjales 1974, Femandes & al. 1977, Femandes & Queiros 1978. Talavera & Arista 1995). On Iy G ilot (1965) had previously found 2n = 46 .

1206. Cylisus villasus Pourret - 2n = 50. 52 (Fig. 5).

Ma: Moyen Al ias, jbel Tazekka, 34°03 ' N, 4"09'W, 3 Jul 1999, Tahiri, RAB62 155. Rif, enrre Bab Taza et Bab Berred, 35"04'N, S"03' W, 6 Jul 1998. Tuhiri, RAB62 157.

This species is distributed in the Mediterranean area, from the northwest of Africa, the nonheast of the lberian Peninsula. the south of France, It al y, and finally to the Balkan Peninsu1a and Turkey. The first sample shows 2n = 52 in root mitosis. Exceplionally, 211 =50 were found in cells from a single rOOL The second sample shows 2/1 = 50 in ali the studied roots. Previous reports indicate two different chrornosome numbers for this species. Sai'!udo ( 1973a) found 2n z 48 in a sample from Spain, and the same number was obtained in sam­ ples [rom Sardinia (Cusma Velari & al. 1999). Forissier (1975) ind icated /I '" 25 in a sam­ pIe from Italy. and Troia & al (1997) 2n = ca. 48 in samples from Sici1y (ltaly). Our results, the fi rst obtained in material from Morocco, show variation in Ihe chromo­ some number ofe. villo5us and add a Ihird chromosome number lo this taxon.

1207. Genisla mOllspessulana (L.) L. Johnson - 211 = 44. 46 & n = 22 (Fig. 6). Ma: Rif, entre Bab Taza et Bab Berred, 34"59'N. 4°45'W, 6 Ju l 1998, Talliri. RA B62170. Maroc atlantique nord, foret au sud de Laraehe. 35°38' N, 6"08' W, 29 May 1998. Tahiri. RAB6216L Flora Mediterranea iO - 2000 417

This taxon is widely diSlributed in North Africa and the soulh and centraI Europe, extending also imo Asia. No data are available for Morocean plants. The only previous records ofthe chromosome number ofthis species are from the lberian Peninsula (2n '" ± 46, by Castro 1949, sub C. monspessulanlls; 2n = 48 by femandes & Santos 1975: and n = 23 by Sanudo 1973c). We have found 2n = 46 ehromosomes in rool mitosis or both sam· ples. However, in the second population there are also plants whieh show 2n = 44 ehro­ mosomes in root mitosis and n = 22 bivalents at meiotic metaphase.

Ac knowledgemenls

This work was panially supportcd by a grant from thc Comunidad Autonoma dc Madrid (CAM. 07B/00 1411997).

References

Castro D. de. 1949: Novas numeros de cromosomas para o género CyfisliS L. - Agron. Lusil. I l: 85-89. Cubas, P., Tahiri, H. & Pardo, C. 2000: Karyological and taxonomiç notes on Cylislis Dcsf. SCCI. Spartopsis Dumort. and Seet. Albumoidcs DC. (Gcnisteac. Leguminosae) from thc lberian Peninsula and Marocco. - BaI. J. Linn. Soc., in presso Cusma Velati, T. , Fooli Chiapella, L.. Bacchetta. G. & Kosovel. V. 1999: Reports (1071 - 1072). [In Kamari, G., Felber. F. & Garbari. F. (ed.). Mediterranean chromosome numbcr reports-9] ­ F1. Mcdit. 9: 36 1-363. Fernandes, A. & Santos, M.F. 1971: Contribution à la connaissanee cytotaxinomique des Spermatophyta du Portugal. - Bol. Soc. Brot. sér. 2. 45: 177-225. - & - 1975: Contribution a la connaissancc cytotaxinomique dcs Spcrrnatophyta du Portugal. IV. Leguminosae (suppl. I). - Bol. Soc. Brot. sér. 2, 49: 173- 196. - . - & Queir6s, M. 1977: Contribution il. la connaissancc cytotaxinomique des Sperrnatophyta du Portuga1. IV. Leguminosae (suppl. 2). Bol. Soc. Brol. sér. 2. 51 : 137-186. - & Queiros, M. 1978: Contribulion il. la connaissance eytolaxinomique des Sperrnatophyta du Portugal. IV. Leguminosae (suppl. 3). - Bol. Soc. Brot. sér. 2 . .5 2: 79· 164. Forissier, R. 1973: Rccherches cytotaxonomiques préliminaircs sur les genrcs Lembotropis. CylisliS. Chamaecylisus, Genisla el Chamaesparfium. - Bull. Soc. Neuchàtc10isc Sçi . NaL 96: 51-65. - 1975: Reports. [In LO'lc A (ed.), IOPA Chomosome number reports XLIX]. - Taxon 24(5/6): 671-678. Francisco-Ortega, J. • FemandeL·GalvM. M. & Santos-Guerra, A. 1992: On Ihe nomcnclature of Chamaecy/isus mollis (Cav.) GreUler & Burdet lFabaccac: Genistcae) and its distribution in the Canar)' Islands. Lcctotypification ofSpartium molle Cav. and Cytisus albidus DC. Candolka: 47: 1·9. Gilot,l. 1965: Contribution à !,étude C)1otaxonomiquc des Gcnistcac et des Lolcae. - La Cellule 65: 317-347. Horjales. M. 1974: Numcros cromos6micos cn Genisteas. - Ana/es del IlIslilufo Botanica Cavanilles 3 1( I): 175· 178. Maire, R. 1987: Flore d' Afrique du Nord. 16. - Paris Sai\udo. A. 1973a: Variabilidad cromosomica dc las Genisteas de la flora cspar'lola en rdacion con su ecologia. 1.- Numero y componamicnto dc los cromosomas durante la mciosis. E. Género Cytisus L. - Bol. Real Acad. Esp. Hisl. Nat. (Biologia) 71: 34 1·355. 1973b: Variabilidad cromos6mica dc las Genislcas dc la flora espanola cn rc1ac i6n con su ecologia. I.. Numero y componamiento dc los cromosomas durante la meiosis. D. Generos Flora Mediterranea lO - 2000 419

Chronanlhus (DC.) C. Koch, Adenocarpus DC. y Erinacea Adanson. - Lagascalia 3(2) : 205- 210. 1973c: Variabilidad cromosomica de las Gcnistcas dc la nOni espanola cn rclacion con su \:cologia. 1.- Numero y comportamicnto dc los cromosomas duronte la mciosi s. C. Scec10n Cephalospanum del Gen. Genisla L. y géneros Lygas Adarnson. SpartiurII L.. Teline Medic .. Calycotome Link y ArgyrolobiurII Ecklin & Zenhyer. - Cuademos de Ciencias Biologicas 2.2 : 117-120. Talavcra. S. & Arista, M. 1995: Numeros eromosomatieos de plantas oecidenta!cs. 712·7 17.­ Anaks Jard. Bol. Madrid 53( 1): 101. Troia, A.. Conte, L. & Cristofolini. G. 1997: Isolalion and biodiversity in Cy/islis villoslIs Pourrel (Fabaeeae. Genisteae): enzyrne polymorphism in disjunel populations. - Plant Biosystcms 131(2): 93 -1 01.

Addrcss of Ihc authors: l-likmat Tahiri. Depanemenl de Biolog ie, l'acuite dcs Sciences. Universi!c Mohamed v, Av. Ibn Balloula, BP 1014. Rabal, Morocco. Paloma Cubas. Depanamento de Biologia Vegetai Il, Facultad de Famlacia, Univcrsidad Complutcnsc. 28040 Madrid. Spain.

Reports (120S-1212) by Dolj a Pavlova & Anita Tosheva

120S. Ononis pusilla L. - 2n = 30 (Fig. l).

Ou : Thracian plain, Besapara hills, calcareous terrains near 10 Ognjanovo village, 42°07' N, 24°2S' E, 150 m a.s.i., 23 Ju1 1998, D. Pavlava, (SO 97805).

The distribution range of this species covers South Europe, the Mediterranean, Ihe Caucasus Mts. and Soulhwest Asia. This species is rarely distribuled in Bulgaria up lo 1000 m a.s.I..(Kuzmanov 1976, Kozuharov 1992). The chromosome number 2n = 30 studied here confinns previous dala from Bulgaria (Kuzmanov & Markova 1973) and from Porruga1 (Femandes & Queiros 1978). Othcr exist­ ing cytotypcs (Majovsky 1970) give us reason to comprehend numbcr 2n = 30 as an ancuploid (2n = 4x-2 = 30). The ann index (R = US) shows chromosomes ofmetacentric and submela­ centric types. The chromosome si:ze is vaned between 3.2 mm and 5.2 mm. The ralio X"'''' : Xmin is 1.75: I. The karyotype is symmetrical consisting of2n '" 22m + 8sm chromosomes.

1209. Ononis adenotricha Boiss. - 2n = 16 + 2B (Fig. 2).

Bu: Thracian plain, Besapara hills, cakareous terrains near IO Ognjanovo village, 42°07' N, 24"28' E. 150 m a.s.i.. 23 Ju11998. D. Pavlava, (SO 97804).

The distribution range of this species covers Ihe Balkan peninsula, Turkey, Syria and Lebanon (Kuzmanov 1976). This plant is included in thc Red Data Book of Bulgaria wilh category "rare" (Velchev 1984). The chromosome number for this population is 2n =- 2x = 16 +28. A previous invest i­ gation of Kuzmanov & Markova (1973) yielded 2n = 16. The karyotype consists of 2n = 2x = 8m + 6sm + 2srn-$AT + 2B. Il ditTers from the 420 Kamari. Felber & Garbari: Mediterranean ehromosome num ber reports - lO

eount2n = 2x = 4m + 6sm + 4a + 2a-5AT ofKunnanov & Man:ova (I.e.) who found acro­ eentrie ehromosomes. Thc arm index shows chromosomes ofmetaeentric and submetacentric types. The ehro­ mosome size is varied between 4.0 mm and 6.4 mm. The ratio Xn\n: Xmin is 1.6 : 1. In ali metaphase plates a pair of submetacentric chromosome with satcllites is fou nd. The salel­ lites are small, ball-shaped and conneeted to tbe short arm of the chromosome. A pair of B-chromosomes Is also observed. This pair is distinguished from olher chromosomes being mueh shorter and probably of submelaeenlrie type.

1210. Ononis arvensis L. - 2n - 30 (Fig. 3).

Bu : Ri la Mts, Eleshnitza village, close to Quercus cerris fore sl. 42°08 ' N, 23°15' E, 650 m a.s.l., 23 Jul 1998, A. Tosheva, (SO 99785).

The distribution range of Ihis species covers Europe, thc Caueasus Mts. Centrai and Southwest Asia, and India. Th is spec ies is widespread in the Bulgarian fl ora up IO 1600 m a.s. 1. (K uzmanov 1976, Kozuharov 1992) The ehromosome number 2n = 30 confirms tbe data of Moriset ( 1978) and Magulaev (1980) for populations from South Europe and Russia. The numbers 2n "" 32 and 2n = 24 were reported by Chuksanova (1967) and Kuzmanov & Markova (1973). The chromosomes are small and the pos ition ofthe centromeres is not expressed in mosl of Ihern. (Fig. 3) The chromosorne size is between 2.4 mm and 4.8 mm. The ralio Xmax : Xmm is 2 : I. A rypical di vision of the chromatides in some chromosomes is observed. Taking into account the basic chromosome numbcr x = 8 the count 2n '" 30 is considered as a resul1 of dcscending aneuploidy.

1211. Melilolus alba Med. - 2 = 16 (Fig. 4, 5).

Uu : Blaek Sea Coast, the town of Balcik, 43OZ2~ N, 28 ° 09~ E, 31 Jul 1999. A. Tos!Je\'o, (SO 100288) Sofia region, western PB;rt ofthe city ofSofia, 42OZ8~ N. 23° 12,. E, 500 m a.s.l.. 28 Jul 1999, A. Tosheva, (SO 100289).

This species is widesprcad in Europe, Bulgaria also included (Kozuharov 1976, 1992). The chromosome number 2n = 16 confinns previous investigations (sce Fedorov 1969: 310. Shopova & Sekovski 1982a: II , Goldblatt 1981: 247, 1984: 188. 1985: 97, 1988: 109, Goldblatt & Johnson 1990: 90, 1991 : 106. 1994: 100, 1996: 117 for references). The num­ bers 2n - 24 (A(Wood 1936) and 2n - 32 (Lesins 1952. Raghuvanshi & al. 1980) are poly­ ploid varianlS OD tbe basis of x=8. The karyoty"pes ofthe (wo investigated populations are similar. They are symmctrical. The on1y difference is a pair of submetacentric chromosomes with satellites found in the population frorn Sofia (Fig. 5). The karyotypes consist of211 "" 2x = 10m + 4sm +2 sm­ SAT = 16 chromosomes (Sofia popu lalion) and 2n = 2x :: 10m + 6sm = 16 (Balcik popu­ lalion). The arm index (R=US) shows mClacentric and submetacentric chromosomes. The chromosome size fro m Sofia population is rangcd between 7.2 mm and 8.8 mm with Flora Mediterranea 10 - 2000 421

approximately minor differences in length between the longest and shortesl chromosome ( xma~ : Xmin is 1.2 : I). This ralio is 1.6 : I for the population from Balcik.

1212. Melilolul" officinalis (L.) PalI. - 2 = 16 (Fig. 6).

Bu: Black Sea Coast, the city ofVarna, St. Konstantin resort. 43°12t N, 27°53t E, 27 Jul 1999, A. Tosheva, (SO 100300).

This species is widespread in Europe, the Caucasus Mts, the Mediterranean, Centrai and Southwest Asia. lt is also common in Bulgaria (Kozuharov 1976, 1992). The chromosome number 2n = 16 is reported for the first time for population from Bulgaria. This chromosome number confinns previous counts (see Fedorov 1969: 3 10, Goldblatt 1981: 248; 1988: 109, Goldblatt & Johnson 1990: 90. 1991: 106, 1994: 100, 1996: Il 7 for references). A tetraploid chromosome number (2n '" 32) is reported by Lesins (1952). The arm index (R = US) gives reasons IO consider the chromosomes being or metacentric and submetacentric types. One ofthe submelacentric pairs has small ball-shaped satellitcs. Two pairs with loose centromeres are found in ali metaphase plates. This is mOSI probably Ùle rea­ son for the observed division ofthe chromosome arros. The karyotype is slightly as}1llmetri­ cal. lt consisls of2n = 2x'" 10m + 4sm + 2sm-SAT '" 16 chromosomes. This karyotype differs mainly from the result of Shopova & Sckovski ( 1982b) by the smal1er number of metacentric chromosomes (l pair) and the higher number of submelacentric chromosomes (7 pairs). The chromosome sizes are between 4.8 mm and 8.8 mm. The ratio Xmax: x min is 1.8 : I.

ACknowledgements

Tbe financial suppon provided by the Sofia Universit)' St. KlimcnI Ohridski Rcscarch Fund - Project 13 269199 is gralefutly acknowledgcd.

References

AIWood, S. 1936. Telrap10id and aneup10id Melilotus alba resutting from hcat trcatmenl. - Amer. louro.l3ot. 23(10): 674-678. Chuksanova, N. 1967: Chromosomnle chisla nekotorlch vidov flori SSSR is scmeistva Leguminosl.'. - Bol. Zum. 52(8): 1124-1131 (In Russian). Femandes. A. & Quciros, M. 1978: Contribution a la conne5sance cytota>;onomique des spermatao­ phyta du Portugal. - 1301. Soc. Brot. Scr. 2 52: 72·164. Go1dblatt, P. 1981: 1ndcx to chromosome numbers for 1975-1978. - Monogr. Syst. Botany. Missouri Bol. Gard. 5. - 1984: Index to pianI ehromosome numbcrs for 1979-1981. - Monogr. Syst. Botan)'. Missouri 801. Gard. 8. - 1985: Index to plant chromosomc numbcrs for 1982-1983. - Monogr. Syst. BOlany. Mi ssouri Bot. Gard. 13. - 1988: Index IO pianI chromosome nllmhcrs for 1984-1985. - Monogr. Syst. BOlany. Missouri Bot. Gard. 23. - & lohnson. D. E. 1990: Index IO plant chromosome numbers far 1986-1987. - Monogr. Syst. Botany, Missouri Bot. Gard. 30. 422 Kamari. Felber & Garbari: Mediterranean chromosome number repons - IO

...... - '...... ~ -- '-.... ~ 1-.~,., -_'... - .. la 2 ...... ­ ,. #, .... : .-...... ,... .. I··...­ l, ·'Il.t.~ e. 4!. ~ .... ,~ ,,, . = ., ~ ... ,.~. .. .­ (... '.!'t ..•••• .. ':0-"~ ..... • • 3 .... 4

Figs. 1-6. Mi tocic me taphasc plates of: I. Carex forruginea. a. 211 - 38: b. 2/1 - 40: 2, C firma. 2/1 - 34: J . C hal/erialla. 2/1 - 50: 4. C. hordeistichas, 2/1 = 54: S, C. mcla/lrutachya. 2/1 ... 76: 6. C michelii. 211 - 62. Flora Mediterranea IO - 2000 423

- & - t99t: Indcx IO pianI chromosomc nllmbcrs lor 1988· 1989. - Monogr. Sysl. BOlany. Missouri Bot. Gard. 40. - & - 1994: Indcx lO pianI chromosome numbcrs for 1990·1991. - Monogr. Sysl. BOlany. Missouri Bot. Gard. 51. - & - 1996: Indcx IO pianI chromosomc numbcrs for 1992· 1993 . - Monogr. Sysl. BOlan}'. Missouri Bot. Gard. 52. Kozuharov. S. 1976: !llelilotus Mi ll. In: Jordanov. D. (cd.). Flora NR Bulgaria. 6: 3 18·327. - BAN. Sofia. - 1992: Faboçeo(' [In Kozuharov, S. (ed.). Opredd ilel na \'ischile raslcnija v Ualgarija] : 382·44 1. - Nauka i Izkuslvo, Sofia KU7.manov. B. 1976: Ol/ol/is L. In: Jordano\'. D. (cd.). Fl ora NR Bulgaria 6: 259·269. - BAN. Sofia. - & Matkova. T. 1973: Repons [In Lo\"e. A. (ed.), IO PB Chomosomc numbcrs rcpons. XL]. Ta.'(on 22 : 288·289. Lesins. K. 1952: Some data on thc c}' logcnclics of alfalfa· dour. - Hcredil)' 43 (6): 287·291 . ~' I agulacv, A. J. 1980: Chromosome numbers of some Fabact'{le in the nonh Caueasus. - Bot. lurn. 65(6):836· 842. Mnjovsky, J. 1970: Index of ehromosomc numbcrs of Slo\'akian flora (Pan I). - Aela Fae. Rcrum. Nat. Univo Comen. Bot. 16: 1·26. Morisct. P. 1978: Chromosornc numbers in OllOlI;S L. scrics l 'ulgares Si rj. - \Vatscnia 12: 1"5· 153. Raghuvanshi S. S .. Nahajan S. & Singh. A.K. 1980: Meiolic bchador IInd fcnilit}' of rnw and ( l'oh'cd auloleuaploid swCCI co\·cr. - New Botanist 7: 1·5. Shopova. M. & Sekoyski. 1.. 1982a Chromosomen atlas of some Mecedonian Angiospcrms. - Maccdonian Acad. Sci. Ans. Skopje:. 4 1pp. . - & - 1982b. Chromosomcn alias of some: Mecedoninn Angiospcnns 11 1. - I\nn. Fac. Biol. Skopje: J5: 147. Vc1chey. V. 1984: Rcd Data l300k of Bul garia I : 197. - BAN. Solia.

Addrcss of the authors: Dr. D. ['a\" lo\'a & A. T(l ~ hcv a. Univcrsity of Sofin ·· St. Kliment Ohridski··. f aculty or Biology. De:panmcnl orBotany. bh"d. Dragan Tzankov 8. 142 1 Sofia. Bulgaria

Reports ( 1213-1226) by Milka S toeva

1213. Curexferruginea Scopo - 211 = 38. 40 ( Figs. la, b).

Bu : Piri n mountain, Dalbokolo dcre gu lly, between Bande rica reslhouse and Vi hren resthouse, 41 °45'N, 23I>2S'E, around running water ofthe gull y, 2200 m, Sloewl S 1635 (SOM) - 2" ~ 38. Pirin mounlain, a gully under Vi hren summil parallel w;th Dalbokoto dc re gully, 4 Io45·N. 23OZS' E, around run ning water oflhe gu lly, 2400-2500 m. SloevG S1631 (SOM) ~ 2n = 40.

This species is ;ncJ uded in Thc Red Data Book of PR Bu lgaria (VeJchev 1984). The chromosome nu mbers found and the karyotypes are like those repo r1ed by Dietrich (1967) ~ 2n = 38 = 6A + 28 + 30C; 2n = 40 = 4A + 2B + 34C. The chromosome number 2n = 58 was also reponed for the same spec ies by Tanaka ( 1942 , 1948). bUI il has nOI been confinned by other authors. 424 Kamari, Felber & Garbari: Medilerranean chromosome number reports - IO

, ... C:'-J- •••-. .. ,. .-~, . e.,'. -,-:... ,.1'. . l''., ,. ...,• I I. 8 • •.. - ,. .".. '-...... 'o.-, \\...... "', \~...... :".. "~' 9 lO ...... "

Figs. 7·11. Mitotic metaphase plat/!S or: 7, C. p(micufafa, 2/1 " 60; 8. C. pel/dlda. 2/1 '" 58: 9, C. pseudocyperus. 21/ '" 66; 1O. Cyperus serafinl/s. 2n '" 46; Il, E/eocllaris qllinquejlora. 2/1 .. 50. Flora Mediterranea IO ~ 2000 425

1214. Cara firma Host ~ 2n - 34 (Fig. 2).

Po: Tatra mountains, Dolina Strazyska va lley, slopes of Trzy kominy. limestone. 490 16 'N, 19056'E, 1000-1100 m, Sloeva S1404 (SOM).

The obtained result confLmls the most literature data (see Dietrich 1967, Fedorov 1969, Murin & Padova 1979, Pogan & Wcislo 1983, Druskevic 1982 cit. in Goldblatt & Johnson 1990). The last author reported 2n = 68 for the same spccies too. The karyotype is similar to that described by Dietrich (1967) ~ 2n = 34 = 6A + 2B + 26C.

1215. Cara Ita/ledana Asso ~ 2n = 50 (F ig. 3).

Bu : Blaek sea eoast (northem), Natural Park "Zlatni pjasaci", 43017'N, 28003'E, oal ­ fores t, dI)' places, 200 m, Sloeva S 1626 (SOM).

The number agrees only with some resulls ofDietrich (1964, 1972) who eounted n = 25 and n = 26 in material trom Italy. Lueeno in 1988 reported n = 26 and in 1992 - n = 28 trom Spain . Kjel1kYist & Uive (1963) and U~ve & Kjellkvist ( 1973) published 2n = 54 from Spain 100.

121 6 Ca re.'r IlOrdeistichos ViII. - 2n = 54 (Fig. 4),

Bu : Sofia region, Kazichene marsh locality, 42039'N, 23 028'E, slampcd places, 550 m, Sloeva 51626 (SOM); Aldomirovci marsh locality near Ihe town Sli vni ca. 42052'N, 23001 'E, stam pcd places around the marsh, 600 m, Stoeva S1323 (SOM). Sredna gora region, Losensla mountain, along the path from the Red Cross IO the peal Polovrak, 42033'N, 23034'E, swamp habitats, 950 m, 5toc"\'a S 1256 (SOM). Blaek sea coast (northem), Ihc village Izgrev district Varna, 430 15'N, 270 45'E, dI)' places on Ihe South ofthe vi11age, limeslone, 350 m, Stoem 5 1604 (SOM).

DitTerenl nwnbers have been reported for this spccies: Helibom (1939) published n '" 27; Tamavsehi (1948) - n '" 27-30; Tanaka (1948) and Luccno ( 1992) - n " 28; Agapova & al. (1990 for refercnces) - 2n - 56. Two chromosomes ofthe karyolype are visible to be 10ngcr Ihan thc others.

121 7. Cara me/anoslachya Bieb. ex Willd. (= C. nl/tans Host) - 2n = 76 (Fig. 5).

Bu : Sofia region, Kazichene marsh locality, 420 39'N, 23028 ' E, swamp habitats, 550 m, Sloeva SI O19 (SOM). Thracian plain, the village Biser near the lown Hannanli, 41 050 ' N, 25058'E, damp meadows near the vil lage, 80 m, Stoeva S16 18 (SOM). The number 2n = 76 was unknown for this species unti I now, but il was nOI new one for secl. Pall/dosae. I counted the same number in C. riparia Cun. (Stoeva 1985) and in C. 426 Kamari, Felber & Garbari: Medilerranean chromosome number reports - IO

12a 12b ~

,.- ~ . . •• ~ • 14 • ,I.• •

FigS. 12-14. Mitotic metaphasc platcs of: 12, 5cirpus lacuslris. a. 2" = 40: b. 211 =42: 13, Sc!U>t'tIllS l1igricUt/J, 211 - 44: 14, Triglochifl palustris. 2/1 = 26. aCllliformis Ehrh. (unpubl. dala). Thrce other chromosomc numbers are known for C. mekm oslachya: Koyama (1962) counled 2" = 82 in malerial from Japan. Chebbotar & al . 1977 (In Agapova & al. 1990) 2,, :: 48 from Moldova and Nijalingappa & al. ( 1978) 2/1 =58 from India. Two cbromosomes orthe karyotype are visiblc longcr than thc others.

12 18. Carex ",ic!,elii Hosi - 211 = 62 (Fig. 6).

Bu : Black sea coast (Northem), the vi llage lzgrev district Vama. 43 0 15 ' N, 27045 ' E, dry shrubs above thc villagc, 350 m, Sioeva S I603 (SOM). Sredna gora region, L07.cnska mountain, bellow the peak Polovrak, 42°33 'N. 23033'E, shrubs of Coryllus ClVellana, 900- 1000 m, SloeVCl $ 1245 (SOM). Slruma valley, Konjavska mountain. the villagc Garbino, 42023 ' N, 220 44'E. dry shrubs ncar thc villagc, 500 m, Sioe\:a S I1 95 (SOM).

The resul! found is in agrccmcnt with thal of Druskevic (1982 ciI. after Goldblau & Johnson 1990) in material from Moldova. Toderash (1979) counlcd a tess chromosome number - 2n = 40 from Moldova 100. Murin & Majovsky (1976) reported 211 = C. 70 in material from Slovakia. Four chromosomcs ofthe karyocype are visible longer than the others. Flora Mediterranea 10 - 2000 427

121 9. Cara pan;culala L. - 2n - 60 (Fig. 7).

8u: Sofia region, Kazichene marsh loca1ity near the village Kazichene, 420 39'N, 23D:z8'E, swamp habitals, 550 m, Slvel,a 51639 (SOM). Toundzha hilly region, Ihe village Alexandrovo, 42035'N, 25005'E, damp meadows near the village, 420 m. Sioeva S 127 1 (SOM).

The oblained chromosome number confinns the resu1ts of Murin & Majovs\.."y (1978). LOve & LOve (198 1). Dietrich ( 1972) reported n = 31. Chromosome numbers 211 = 62 and 2n " 64 were pub1ished for this species too (see Moore 1982 for referenees).

1220. Cara pendula Hudson - 2n = 58 (Fig. 8).

Bu: Black sea coast (Nonhcm), Natural Park "Zlatni pjasaci", 430 17'N, 28003 ' E, damp shady places in me oakforesl, 200 m, Sloe>.'Q 51629 (SOM). Sredna gora region, Lozenska mountain, along the roule from the Red Cross lo the C loister. 42°33' N, 23°34' E. damp shady places among hombeam wood. 850 m. SlDeVa S I284 (SOM).

The ehromosome number eSlablished is me same reponed by Murin (see Moore 1982 for references) in materia1 trom Slovakia and Strid & Franzen (1981) in materia1 from Greece. Oltonello & al. ( 198 5 ciI. afler Goldblatt & Johnson 1990) published 211 = 60 and Druskevic (1982 ciI. after Goldb1att & Johnson 1990) - 211 '" 62. Chebbolar (1977 ciI. aflcr Agapova & al., 1990) counted 211 ::: 58 and 211 = 62 from Motdova. Two chromosomes of the karyotype are much 10nger than the others.

122 1. Care.\" pseudocyperus L - 2n ::: 66 (Fig. 9).

Bu: Sofia region, Kazichenc marsh locatity. 420 39 'N, 23 0 28 'N, 011 Ihe side ofa drain waler, 550 m, SlDeva 5 1638 (SOM).

The chromosome numbcr found agrces w ith the li teratu re dala for this species spccies (Koyama 1962, Uhe & Lave 198 1).

1222. Cyperus serot;nus ROllb. (= Pycrells serolinllS (Ronb.) Hayek) - 2/1 = 46 (Fig. IO)

Du : Danube plain, on the north ofthe to",n Mizia, 43038' N. 23055" E. on the side of Skat river, 100 m, Sloe\'Q 5 1645 (SOM). To my knowledge, the fi rst kal)'ological sludy for Ihis speeies.

1223, Eleocll aris qu;"queflora (F. X. Hanm.) O. Schwarz (= E. pauciflora Roemer & Schu1tes) - 211 = 50 ( Fig. II). 428 Kamari, Felber & Garbari: Medilerranean ehromosome numher repo l1S - lO

Bu: Ri la mountain, the village Mala Cherkva district Pemik. 420 13'N, 23 030'E. feo near the village, 1100 m, Sl oeva SI655 (SOM). Pirin mountain, Todorini ezero lakes, below thc pcak Todorka, 4 10 44'N, 2]°27'E, swamp habitats around thc lakc, 2500 m, Sloeva 51440 (SOM). Toundzh.a hilJy region, thc villagc Gabarcvo ncar Ihe town Kazanluk, 420 37'N, 25009'E, swamps ocar Ihc villagc, 430 m, 510eva 5 1658 (SOM).

Thc chromosome number 2n = 50 was unknown so far for Ihis specics, Davies (1956) counled n = IO. Some much greater numbers have been repol1cd 100: 2n = 80·100 (sce Fedorov 1969) and 2n "" 132, 134, 136 (sce Moore 1982 for rcferenccs). The number 2n = 50 was reportcd for E. paluslris by Tamavsehi (1948). The karyorype of E. quinqueflora is vcf)' helcrogcneous in relative length ofthe ehro­ mosomes and Ihus il is similar to the karyotype of E. palustris with 2n = 16 chromosomes.

1224. Scirpus lacustris L (::::: 5choelloplectus lacustris (L) Palla) - 2n = 40. 42 (Figs. 12a, b).

Su: Danube plain, the village Dekov oear me town SvishlOV, 430 36' N, 25007'E. damp placcs ncar by Cemilica marsh, 50- 100 m. 5lOeva S760 (SOM) - 20 = 40. North eastem Bulgaria, Srebama reserve. 44006'N. 27 0 04 'E. damp places ncar by the lake Srebarna, 50 m, Stoeva 5783 (SOM) - 20 = 42.

The chromosome nu mbers couoted agree wi th thc literaturc dala (sec Fedorov 1969 and Lnve & Ll'Ive 1974). Thc nmnncr 2n " 42 was also reported by Va chova (sce Moore 1982 and LOve & LOve 1981 , for refercnces). Two chromosomes of the eytQ(ype with 2n ; 40 are much longer than the others.

1225. ScllOenus nigricons L. - 2n = 44 (Fig. 13).

Bu : Toundzha hill y region, the village Gabarevo ocar the town Kazao luk. 420 37'N. 25 0 09' E. swamps near tbe village, 430 m, Sfoeva 51657 (SOM).

This resull agrees wi th the data of Davies ( 1956) for Great Bri lain, Labadie ( 1976) and Natarajan (1978) for France. The greater chromosome numbcr - 2n = 54 has bccn reponed for the same specics [00 (see U),ve & LOve, 1974 and Moore 1982 for refer­ cnccs).

1226. Trlglochin poluslris L. - 2n - 26 (Fig. 14). Bu: Sofia region, Kazichene marsh locality, 420 39' N, 23~ 8 ' E, swamp p[aces. 550 m, SlOevaSI643 (SOM).

The most aUlhors reported 211 = 24 for this species (see Fedorov 1969, LOve & Ltlve flora Mediterranea IO - 2000 429

1974, Krasnikov 1991 and Hol1ingsworth & al. 1992). Sokolovskaya (1 963 cito after Fedorov 1969) found 2n = 24 - 26. The karyotype consists ofholocentric short chromosomes.

Aeknowledgments

Fi nancial suppon by Ihe National Science Fund, Minislry of Education and Scicnce. Republic of Bu lgaria. project B-702 is gralefu!1y acknow[edged.

References

Agapova, N. D., Arkharova, K. B .. Vakhtina. L. I.. Zcmskova, E. A. & Tarvis. L. V. 1990: Numeri chromosomatum Magnoliophylorum Fl orac UR$S: Aceraceae - Menyalll/wceae. - Leningrad. Davies, E. W. 1956: Cy tology, evo[ution and orgin of the aneuploid scries of Care.r (C'.l'peraceae). - Hercditas 42: 349-]65. Dietrich, W. 1%4: Documented chromosome numbers ofp1ants. - Madrono 17: 266-268. - 1967: Die ZYlotaxonomie dcr Carex - Seelion Frigidae in Europe. - Fecds Repart. 75: 1-42 . - 1972: Reports [In LOvc, A. (ed). IOPB ehromosome numbcr reports XXXVI I. - Taxon 21 : 33]- 335. Fedorov. A. N. (ed) 1969: Chromosome numbers of flowering plants. - Lcningrad. Go[db[atl, P. & l ohnson, D. E. [990: Inde" IO p[anl chromosome numbers for [986- [987. Monogr. Sysi. 130t. Missouri Bot. Gard. 30. He [i born, D. 1939: Chromosome studies in Cyperaccae. lII-IV. - Hereditas 25: 224-240. Hollingsworth. P. M., Gornall, R. J. & Baite)', 1. P. 1992: Contribution IO a cyto[ogical eataloguc of the British and Irish flora. 2. - \Valsonia 19: 134-137. Kjcllk\'isl, E, & LOve, A. 1%3: Chromosome numbers of some Cm'ex spccics from Spain. - Bot. Nat. 116: 241 ·248. Koyama, T. 1962: Classificalion oflhe Fami ly Cypeaceae (2). - Journ. Fac. Sci Univo ofTokyo. sect. Bot. 8: 149-278. Krasnikov, A. A. 1991: Chromosome numhcrs in some spccics of vascular plants l'rom Novosibirsk region. - BaI. Zhum .. 76: 476-479. Labadie, J. P. 1976: Reports [In U~vc. A. (cd), !OPB chromosome numhcr reports L1 V] - Taxon. 25: 636-639. Lovc. A. & Kjcllkvisl, E. 197]: Cylola.'\onOnl)' of Spanish Plants. Il. Monocoty ledons. - Lagascalia J : 147-1 82. - & LOvc. D. (cds.) 1974: CYlotaxonomical alIas ofthe Slovenian Flora. - Leh re. - & - 1981 : Repons [In LOve, A. (ed), IDPB chromosome number rcports LXXIII] - Taxon 30: 845-851. Luceno, M. 1988: Notas cariologicas II I. - Anal. lardo Bot. Madrid 45: 189-196. 1992: C)10taxonomic sludies in Iberian and Macaronesian species of Care.T (Cyperaceae). Wi lldenowia 22 : 149-165. Majovsky, J & Murin, A. 1987: Karyolaxonomicky prchl ad fiol)' Slovenska. - Bratislava. Moorc, D. M. (ed.) 1982: Flora Europaea check-lisI and chromosome indcx. - Cambridge. Murin, A. & Majovs ky, J. 1976: Reports. [In LOvc, A. (cd). IOPB chromosome numbcr rcports. LIII]. - Taxon 25: 487-488. - & - 1978: Rcports Un U~ve, A. (ed). IDPB chromosome number reports LX I] . - Ta'\On. 27: 376-378. - & Paclova, L. 1979: Rc parts [In Love. A. (ed), IDrB chromosomc numbcr rcports LXIV ].­ Taxon 28: 403-405. 430 Kamari, Felber & Garbari: Mediterranean chromosomc number reports - IO

Natarajan, G. 1978: Rcpons [In LO\'c. A. (C1.i). IOPB chromosomc numbcr rcpons LX II[. ~ Taxon 27: 526-531. Nijalingappa. B., Nagaraj. N. & Tejavathi. D. 1978: Rcports [I n Ujvc. A. (ed.). IO PB chromosomc numbcr rcpons LXlll. - Taxon 27: 53 1-532. POgWl. E. & Wcislo. H. 1983: A li sI or chromosornc numbers of 1'0lish Angiosperms. II. - Acta Iliol. Cracov., Ser. Bot. 25: 103-172. Sloeva. M. 1985: Chromosomc numbers ofBulgarian Angiospcnns. - Fitologija Solia 30: 78-79. Strido A. & Frnnzen. R. 1981 : Rcpons Ii n Uh·c. A. (ed). IOPB chromosome number rcpons LXX I­ III. - Taxon 30: 829-861. Tanaka. N. 1942: Chromosornc studics in Cypcraccac. XVlll. Chromosome numbers of Carex (Primoc:ort'x). - Mcd. and BioL, 2: 210-214. - 1948: Thc prob1cm or ancuploidy (Chromosornc studies in Cypcraccac with sJK."Cia l r.:fcrenee IO Ihe prob1ern oraneuploidy). - Biol. COlltr. Japan 4: 1-327. Tarnnvschi. I. T. 1948: Die chrOlllosomcn zah1cn der Anthophyten Flora Rum!!nien mil cilu:n Ausblick aufdas Polyploidic Prob1cm. - l3u 1. Grad. Bot. Univ, Cluj 28. Suppl. I: l-3D. Todcr,lsh. L G. 1979: Chromosomc numbers of fouT spccies of the gcnus Carex L. - Izv. Akad. Nauk Moldovsk. SSR. Ser. Biol. Ki shincv 4: 82-87. Vc1chc\'. V. (cd.). 1984: Red Dala Book oflhe J>coplc's Republk of Bulgaria. VoI. I. Plants. - Sofia, (In Bulgarian)

Address of the author. M. Slocva. Thracian Univcrsity. Aggr. Faculty, Dt:partmenl or Biology. BG-600 Stara Zagora. Bulgaria.