plants Article Collagenase and Tyrosinase Inhibitory Effect of Isolated Constituents from the Moss Polytrichum formosum Raíssa Volpatto Marques 1, Agnès Guillaumin 2, Ahmed B. Abdelwahab 2 , Aleksander Salwinski 2, Charlotte H. Gotfredsen 3, Frédéric Bourgaud 2,4 , Kasper Enemark-Rasmussen 3, Sissi Miguel 4 and Henrik Toft Simonsen 1,* 1 Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltoft Plads 223, 2800 Kongens Lyngby, Denmark;
[email protected] 2 Plant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandœuvre-lès-Nancy, France;
[email protected] (A.G.);
[email protected] (A.B.A.);
[email protected] (A.S.);
[email protected] (F.B.) 3 Department of Chemistry, Technical University of Denmark, Kemitorvet 207, 2800 Kongens Lyngby, Denmark;
[email protected] (C.H.G.);
[email protected] (K.E.-R.) 4 Cellengo, 19 Avenue de la Forêt de Haye, 54500 Vandœuvre-lès-Nancy, France;
[email protected] * Correspondence:
[email protected] Abstract: Mosses from the genus Polytrichum have been shown to contain rare benzonaphthoxan- thenones compounds, and many of these have been reported to have important biological activities. In this study, extracts from Polytrichum formosum were analyzed in vitro for their inhibitory properties Citation: Marques, R.V.; Guillaumin, on collagenase and tyrosinase activity, two important cosmetic target enzymes involved respectively A.; Abdelwahab, A.B.; Salwinski, A.; in skin aging and pigmentation. The 70% ethanol extract showed a dose-dependent inhibitory effect Gotfredsen, C.H.; Bourgaud, F.; against collagenase (IC50 = 4.65 mg/mL). The methanol extract showed a mild inhibitory effect Enemark-Rasmussen, K.; Miguel, S.; of 44% against tyrosinase at 5.33 mg/mL.