Received: 7 May 2020 | Revised: 18 July 2020 | Accepted: 24 July 2020 DOI: 10.1002/edn3.125 ORIGINAL ARTICLE Efficient assessment of nocturnal flying insect communities by combining automatic light traps and DNA metabarcoding Vanessa A. Mata1 | Sónia Ferreira1 | Rebeca M. Campos1 | Luís P. da Silva1 | Joana Veríssimo1,2 | Martin F. V. Corley1 | Pedro Beja1,3 1CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Abstract Universidade do Porto, Vila do Conde, Increasing evidence for global insect declines is prompting a renewed interest in the Portugal survey of whole insect communities. DNA metabarcoding can contribute to assess- 2Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, ing diverse insect communities over a range of spatial and temporal scales, but ef- Portugal forts are still needed to optimize and standardize procedures. Here, we describe and 3CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, test a methodological pipeline for surveying nocturnal flying insects, combining auto- Instituto Superior de Agronomia, matic light traps and DNA metabarcoding. We optimized laboratory procedures and Universidade de Lisboa, Lisboa, Portugal then tested the methodological pipeline using 12 field samples collected in northern Correspondence Portugal in 2017. We focused on Lepidoptera to compare metabarcoding results with Vanessa A. Mata, CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos those from morphological identification, using three types of bulk samples produced Genéticos, Universidade do Porto, Campus from each field sample (individuals, legs, and the unsorted mixture). The customized de Vairão, Vila do Conde, Portugal. Email:
[email protected] trap was highly efficient at collecting nocturnal flying insects, allowing a small team to operate several traps per night, and a fast field processing of samples for subse- Funding information Energias de Portugal (EDP); Agência quent metabarcoding.