DDT HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

Catalog # : L066T6 規格 : [ 100 ug ] List All

Specification Application Image

Transfected HEK293 Western Blot Cell Line: Immunoprecipitation

Plasmid: pCMV-DDT full length

Host: Human

Theoretical MW 13 (kDa):

Lysis Buffer: Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Concentration: 2 mg/ml

Quality Control Transient overexpression cell lysate was tested with Anti-DDT antibody Testing: () by Western Blots. SDS-PAGE Gel

DDT transfected lysate Western Blot

Lane 1: DDT transfected lysate ( 13 KDa). Lane 2: Non-transfected lysate.

Recommend Use it directly for immuno-precipitation, or heat lysate with SDS gel Usage: loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

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Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing. Instruction:

Applications Western Blot

Immunoprecipitation Protocol Download

Gene Information

Entrez GeneID: 1652

GeneBank BC005971 Accession#:

Protein AAH05971 Accession#:

Gene Name: DDT

Gene Alias: DDCT

Gene D-dopachrome tautomerase Description:

Omim ID: 602750

Gene Ontology: Hyperlink

Gene Summary: D-dopachrome tautomerase converts D-dopachrome into 5,6- dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq

Other D-dopachrome decarboxylase Designations:

Related Disease Cardiovascular Diseases Diabetes Mellitus, Type 2 Edema

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