DDT Antibody (R30113)

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DDT Antibody (R30113) Catalog No. Formulation Size R30113 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request Availability 1-3 business days Species Reactivity Human Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Antigen affinity Buffer Lyophilized from 1X PBS with 0.025% sodium azide UniProt P30046 Applications Western blot : 0.5-1ug/ml IHC (FFPE) : 0.5-1ug/ml ELISA : 0.1-0.5ug/ml (human protein tested) Limitations This DDT antibody is available for research use only. Western blot testing of human recombinant protein with DDT antibody. IHC staining of FFPE human placenta with DDT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing. Description DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10. Application Notes The stated application concentrations are suggested starting amounts. Titration of the DDT antibody may be required due to differences in protocols and secondary/substrate sensitivity. Immunogen Human DDT recombinant protein (AA 1-118) was used as the immunogen for this DDT antibody. Storage After reconstitution, the DDT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:[email protected] Copyright © NSJ Bioreagents. All rights reserved.

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Research Article Human Environmental Disease Network: a Computational Model to Assess Toxicology of Contaminants1
ALTEX Online first published October 21, 2016 https://doi.org/10.14573/altex.1607201 Research Article Human Environmental Disease Network: A computational model to assess toxicology of contaminants1 Olivier Taboureau1,2,3and Karine Audouze1,2 1INSERM UMR-S973, Molécules Thérapeutiques in silico, Paris, France; 2 University of Paris Diderot, Paris, France; 3Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark Summary During the past decades, many epidemiological, toxicological and biological studies have been performed to assess the role of environmental chemicals as potential toxicants for diverse human disorders. However, the relationships between diseases based on chemical exposure have been rarely studied by computational biology. We developed a human environmental disease network (EDN) to explore and suggest novel disease-disease and chemical-disease relationships. The presented scored EDN model is built upon the integration on systems biology and chemical toxicology using chemical contaminants information and their disease relationships from the reported TDDB database. The resulting human EDN takes into consideration the level of evidence of the toxicant-disease relationships allowing including some degrees of significance in the disease-disease associations. Such network can be used to identify uncharacterized connections between diseases. Examples are discussed with type 2 diabetes (T2D). Additionally, this computational model allows to confirm already know chemical-disease links (e.g. bisphenol A and behavioral disorders) and also to reveal unexpected associations between chemicals and diseases (e.g. chlordane and olfactory alteration), thus predicting which chemicals may be risk factors to human health. With the proposed human EDN model, it is possible to explore common biological mechanism between two diseases through chemical exposure helping us to gain insight into disease etiology and comorbidity.
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