DDT HEK293 Cell Transient Overexpression Lysate(Non-Denatured) Catalog # : L066T6 規格 : [ 100 ug ] List All Specification Application Image Transfected HEK293 Western Blot Cell Line: Immunoprecipitation Plasmid: pCMV-DDT full length Host: Human Theoretical MW 13 (kDa): Lysis Buffer: Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. Concentration: 2 mg/ml Quality Control Transient overexpression cell lysate was tested with Anti-DDT antibody Testing: () by Western Blots. SDS-PAGE Gel DDT transfected lysate Western Blot Lane 1: DDT transfected lysate ( 13 KDa). Lane 2: Non-transfected lysate. Recommend Use it directly for immuno-precipitation, or heat lysate with SDS gel Usage: loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. Page 1 of 2 2016/7/25 Storage Buffer: In modified RIPA Lysis Buffer. Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing. Instruction: Applications Western Blot Immunoprecipitation Protocol Download Gene Information Entrez GeneID: 1652 GeneBank BC005971 Accession#: Protein AAH05971 Accession#: Gene Name: DDT Gene Alias: DDCT Gene D-dopachrome tautomerase Description: Omim ID: 602750 Gene Ontology: Hyperlink Gene Summary: D-dopachrome tautomerase converts D-dopachrome into 5,6- dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq Other D-dopachrome decarboxylase Designations: Related Disease Cardiovascular Diseases Diabetes Mellitus, Type 2 Edema 服務條款 | 隱私權政策 | 著作及商標 | 網站地圖 ©2016 亞諾法生技股份有限公司 Abnova Corporation. 版權所有. Page 2 of 2 2016/7/25.