bioRxiv preprint doi: https://doi.org/10.1101/437590; this version posted October 8, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Optimised DNA extraction and library preparation for minute arthropods: application 2 to target enrichment in chalcid wasps used for biocontrol. 3 4 Astrid Cruaud1*, Sabine Nidelet1, Pierre Arnal1, 2, Audrey Weber3, Lucian Fusu4, Alex 5 Gumovsky5, John Huber6, Andrew Polaszek7 and Jean-Yves Rasplus1 6 7 1 CBGP, INRA, CIRAD, IRD, Montpellier SupAgro, Univ Montpellier, Montpellier, France 8 2 ISYEB–UMR 7205 MNHN, CNRS, UPMC, EPHE, Sorbonne Universités, Paris, France 9 3 AGAP, INRA, CIRAD, Montpellier SupAgro, Univ Montpellier, Montpellier, France. 10 4 Faculty of Biology, Alexandru Ioan Cuza University, Iasi, Romania 11 5 Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, Kiev, 12 Ukraine 13 6 Natural Resources Canada, c/o Canadian National Collection of Insects, Ottawa, Canada 14 7 Department of Life Sciences, Natural History Museum, London, UK 15 16 *corresponding author:
[email protected] 17 18 running headline = target-enrichment in minute wasps 19 20 Abstract 21 Enriching subsets of the genome prior to sequencing allows focusing effort on regions that are 22 relevant to answer specific questions. As experimental design can be adapted to sequence 23 many samples simultaneously, using such approach also contributes to reduce cost. In the 24 field of ecology and evolution, target enrichment is increasingly used for genotyping of plant 25 and animal species or to better understand the evolutionary history of important lineages 1 bioRxiv preprint doi: https://doi.org/10.1101/437590; this version posted October 8, 2018.