DOCSLIB.ORG

Information to Users

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Information to Users INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of tftis reproduction is dependent upon the quality of the copy sutHnitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. in the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with smal overlaps. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9* black and white photographic prints are available for any photographs or Uustrations appearing in this copy for an additional charge. Contact UMI directly to order. ProQuest Information and Learning 300 North Zeeb Road, Atm Arbor, Ml 48106-1346 USA 800-521-0600 UMT MOLECULAR ANALYSIS OF L7/PCP-2 MESSENGER RNA AND ITS INTERACTING PROTEINS DISSERTATION Presented in Partial Fulfillment of the Requirements for The Degree Doctor of Philosophy in Ohio State Biochemistry Program of The Ohio State University By Xulun Zhang, M.S. ***** The Ohio State University 2001 Dissertation Committee: Approved by Professor John D. Oberdick, Adviser Professor Kathleen Boris-Lawrie - Professor Michael Ostrowski Adviser Professor Harald Vaessin Ohio State Biochemistry Program UMI Number 3031292 UMI" UMI Micrafbrm 3031292 Copyright 2002 by Bell & HoweM Information and Learning Company. All rights reserved. This microfbrm edition is protected against unauthorized copying under Title 17, United States Code. Bell & Howell Information and Laammg Company 300 North Zeeb Road P.O. Box 1346 Arm Arbor. Mi 48ia6-1346 ABSTRACT L7 protein is a G protein a subunit modulator that is specifically expressed at high levels in cerebellar Purkinje cells. L7 mRNA has been shown to be localized in both cell bodies and dendrites in Purkinje cells, but the mechanisms of L7 mRNA localization, translation and the regulation of its translation in dendrites remain unclear. Here we present data to show that L7 mRNA has two different transcripts due to the choice of two alternative first exons, and this property is A conserved in rodent and in human. Both transcript forms are found in both cell bodies and dendrites in human and in rodent, and the distribution of the two transcripts in Purkinje cells is developmentally regulated. These two transcripts in turn will give rise to two L7 protein isofdnns. The shorter form contains one GoLoco motif, a motif which has been shown to interact with GDP-bound Go, whereas the longer form has two GoLoco motik. A ds-element which is responsible for the formation of an RNA-protein complex has been identified in the L7 3' UTR, and this element is conserved in rodent and in human. Two of the protein components of the RNA-protein complex have been partially purified by FPLC and they have been identified as CGI-145 and UNR protein. CGI-145 has no known function so far, but UNR has been shown elsewhere to be involved in cap-independent translational control and/or mRNA stability. Dedicated to my family III ACKNOWLEDGMENTS I wish to thank my adviser, Dr. John Oberdick , for intellectual support, encouragement, and enthusiasm which made this thesis possible, and for his patience in correcting both my stylistic and scientific errors. I would like to thank my dissertation committee members. Dr. Michael Ostrowski, Dr. Harald Vaessin and Dr. Kathleen Boris-Lawrie for their support and inspiring discussions. I would like to thank the Ohio State Biochemistry Program and the Ohio State University for their support. I would like to thank all of my colleagues Dr. Stephan Baader, Dr. Feng Bian, Dr. Teresa Chu, Yufang Zhang and Hailing Zhang for their help in the experiments and inspiring discussions. I would like to thank my wife Haidong and my daughter Yaning for their support during the whole work. IV VITA March 11,1966 ..................................................................Bom in Tianjin, P.R.China 1984 - 1988 .................................................... B.S. Biochemistry, NanKai University 1988 -1 9 9 1 ....................................................M.S. Biochemistry, Nankai University 1991 -1 9 9 5 ....................................................Research Assistant, Chinese Academy of Medical Sciences 1995 - present .............................................. Graduate Research Associate, The Ohio State University PUBUCATIONS 1 ) Zhang,X.; Baader,S.L; Bian,F.; Muller,W.; Oberdick,J. High level Purkinje cell specific expression of green fluorescent protein in transgenic mice Histochem.Cell Biol. 115,455-464,2001 2) Baader, S. L, Vogel, M. W., Sanlioglu, S., Zhang, X., Oberdick, J., Selective disruption of "late onset* sagittal banding patterns by ectopic expression of Engrailed-2 in cerebellar Purkinje cells. J. Neurosci. 19, 5370- 5379,1999 3) Sanlioglu, S., Zhang, X., Baader, S. L, Oberdick, J., Regulation of a Purkinje cell-specific promoter by homeodomain proteins: repression by Engrailed-2 vs. synergistic activation by HoxaS and Hoxb7. J. Neurobiol. 3 6 ,559- 571, 1998 4) Zhang, X., Wang, Y., Streptomyces mycarofaciens midecamycin 4*-0- propionyltransferase gene structure. Weishengwu Xuebao 36,417-422,1996 5) Gao, C., Zhang, X., Cai, B., Li, X., Qiao, M., Zhang, X., Copy number determination and restriction map of plasmid pNK866. Weishengwu Xuebao 34, 81-84, 1994 6) Yang, Y., Zhang, X., Yu, Y., Guo, X., Study on immobilization of DNA on VT copolymer. Lizi Jiaohuan Yu Xifu 6,172-177,1990 FIELDS OF STUDY Major Field: Biochemistry VI TABLE OF CONTENTS Abstract ........................................................................................................................ii Dedication .................................................................................................................... iii Acknowledgments ...................................................................................................... iv Vita ............................................................................................................................... V List of Tables ............................................................................................................ viii List of Figures ............................................................................................................. ix Chapters: 1. Introduction............................................................................................................1 1.1 Cerebellum serves as an Ideal model for the study of the nervous system ..............................................................................................1 1.2 L7/pcp-2 is specifically expressed in cerebellar Purkinje cells ................... 4 1.2.1 Discovery of L7 gene ..............................................................................4 1.2.2 L7 expression in cerebellum is regulated spatially and temporally, and L7 promoter is a valuable cell type specific expression tool 5 1.3 The expression of L7 is regulated by thyroid hormone and other factors..9 1.3.1 RORa regulates L7 expression .............................................................. 9 1.3.2 Evidence of other L7 gene regulators ................................................. 10 VII 1.4 L7 protein interacts with Goi and Goo modulating GDP release from Go...................................................................................................... 11 1.4.1 L7 knockout animals showed no significant phenotype ................... 11 1.4.2 L7 protein interacts with Go subunit .................................................. 12 1.5 L7 protein contains GoLoco motif ..............................................................14 1.6 RGS proteins belong to a protein kwnily and undertake multiple functions including GAP and GDI .............................................................................. 15 1.6.1 RGS proteins are stemmed from yeast Sst2 protein and are present in other lower eukaryotic organisms .................................................. 16 1.6.2 Mammalian RGS proteins are grouped into subfamilies ..................17 1.7 AGS proteins .............................................................................................. 23 1.8 Other GoLoco motif-containing proteins ...................................................24 1.9 GoLoco motif is the interaction site for Gasubunit and it acts as GDI 25 1.10 L7 mRNA is localized in both cell body and dendrites in Purkinje cells..28 1.11 Cis-elements and trans-factors mediate mRNA translocation ............... 30 1.11.1 Cis-eiement is required for mRNA translocation in neurons ......... 31 1.11.2 Protein factors are involved in RNAIocalization in dendrites ......... 32 1.12 Local protein synthesis at synapse in dendrites ....................................33 1.13 Work presented here ............................................................................... 36 2. Material and methods .....................................................................................
Load more

Recommended publications
  • DM Domain Proteins in Sexual Regulation 875 Transformation Marker Prf4 (Containing the Mutant Rol-6 Allele Green fluorescent Protein (GFP) Coding Region
    Development 126, 873-881 (1999) 873 Printed in Great Britain © The Company of Biologists Limited 1999 DEV5283 Similarity of DNA binding and transcriptional regulation by Caenorhabditis elegans MAB-3 and Drosophila melanogaster DSX suggests conservation of sex determining mechanisms Woelsung Yi1 and David Zarkower1,2,* 1Biochemistry, Molecular Biology and Biophysics Graduate Program, and 2Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA *Author for correspondence (e-mail: [email protected]) Accepted 17 December 1998; published on WWW 2 February 1999 SUMMARY Although most animals occur in two sexes, the molecular transcription, resulting in expression in both sexes, and the pathways they employ to control sexual development vary vitellogenin genes have potential MAB-3 binding sites considerably. The only known molecular similarity upstream of their transcriptional start sites. MAB-3 binds between phyla in sex determination is between two genes, to a site in the vit-2 promoter in vitro, and this site is mab-3 from C. elegans, and doublesex (dsx) from required in vivo to prevent transcription of a vit-2 reporter Drosophila. Both genes contain a DNA binding motif called construct in males, suggesting that MAB-3 is a direct a DM domain and they regulate similar aspects of sexual repressor of vitellogenin transcription. This is the first development, including yolk protein synthesis and direct link between the sex determination regulatory peripheral nervous system differentiation. Here we show pathway and sex-specific structural genes in C. elegans, and that MAB-3, like the DSX proteins, is a direct regulator of it suggests that nematodes and insects use at least some of yolk protein gene transcription.
    [Show full text]
  • A W-Linked DM-Domain Gene, DM-W, Participates in Primary Ovary Development in Xenopus Laevis
    A W-linked DM-domain gene, DM-W, participates in primary ovary development in Xenopus laevis Shin Yoshimoto*, Ema Okada*, Hirohito Umemoto*, Kei Tamura*, Yoshinobu Uno†, Chizuko Nishida-Umehara†, Yoichi Matsuda†, Nobuhiko Takamatsu*, Tadayoshi Shiba*, and Michihiko Ito*‡ *Department of Bioscience, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan; and †Division of Genome Dynamics, Creative Research Initiative ‘‘Sousei,’’ Hokkaido University, North10 West8, Kita-ku, Sapporo, Hokkaido 060-0810, Japan Communicated by Satoshi Omura,¯ The Kitasato Institute, Tokyo, Japan, December 27, 2007 (received for review September 11, 2007) In the XX/XY sex-determining system, the Y-linked SRY genes of In some species of amphibians, sex determination is controlled most mammals and the DMY/Dmrt1bY genes of the teleost fish genetically (13), even though the animals’ sex chromosomes are medaka have been characterized as sex-determining genes that morphologically indistinguishable from the autosomes. The trigger formation of the testis. However, the molecular mechanism South African clawed frog Xenopus laevis uses the ZZ/ZW of the ZZ/ZW-type system in vertebrates, including the clawed frog system, which was demonstrated by backcrosses between sex- Xenopus laevis, is unknown. Here, we isolated an X. laevis female reversed and normal individuals (14), but its sex chromosomes genome-specific DM-domain gene, DM-W, and obtained molecular have not yet been identified. Moreover, as with other animals evidence of a W-chromosome in this species. The DNA-binding that use the ZZ/ZW system, no sex-determining gene(s) has domain of DM-W showed a strikingly high identity (89%) with that been identified.
    [Show full text]
  • Noelia Díaz Blanco
    Effects of environmental factors on the gonadal transcriptome of European sea bass (Dicentrarchus labrax), juvenile growth and sex ratios Noelia Díaz Blanco Ph.D. thesis 2014 Submitted in partial fulfillment of the requirements for the Ph.D. degree from the Universitat Pompeu Fabra (UPF). This work has been carried out at the Group of Biology of Reproduction (GBR), at the Department of Renewable Marine Resources of the Institute of Marine Sciences (ICM-CSIC). Thesis supervisor: Dr. Francesc Piferrer Professor d’Investigació Institut de Ciències del Mar (ICM-CSIC) i ii A mis padres A Xavi iii iv Acknowledgements This thesis has been made possible by the support of many people who in one way or another, many times unknowingly, gave me the strength to overcome this "long and winding road". First of all, I would like to thank my supervisor, Dr. Francesc Piferrer, for his patience, guidance and wise advice throughout all this Ph.D. experience. But above all, for the trust he placed on me almost seven years ago when he offered me the opportunity to be part of his team. Thanks also for teaching me how to question always everything, for sharing with me your enthusiasm for science and for giving me the opportunity of learning from you by participating in many projects, collaborations and scientific meetings. I am also thankful to my colleagues (former and present Group of Biology of Reproduction members) for your support and encouragement throughout this journey. To the “exGBRs”, thanks for helping me with my first steps into this world. Working as an undergrad with you Dr.
    [Show full text]
  • Ubiquitin-Dependent Regulation of a Conserved DMRT Protein Controls
    RESEARCH ARTICLE Ubiquitin-dependent regulation of a conserved DMRT protein controls sexually dimorphic synaptic connectivity and behavior Emily A Bayer1, Rebecca C Stecky1, Lauren Neal1, Phinikoula S Katsamba2, Goran Ahlsen2, Vishnu Balaji3, Thorsten Hoppe3,4, Lawrence Shapiro2, Meital Oren-Suissa5, Oliver Hobert1,2* 1Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, United States; 2Department of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, United States; 3Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany; 4Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany; 5Weizmann Institute of Science, Department of Neurobiology, Rehovot, Israel Abstract Sex-specific synaptic connectivity is beginning to emerge as a remarkable, but little explored feature of animal brains. We describe here a novel mechanism that promotes sexually dimorphic neuronal function and synaptic connectivity in the nervous system of the nematode Caenorhabditis elegans. We demonstrate that a phylogenetically conserved, but previously uncharacterized Doublesex/Mab-3 related transcription factor (DMRT), dmd-4, is expressed in two classes of sex-shared phasmid neurons specifically in hermaphrodites but not in males. We find dmd-4 to promote hermaphrodite-specific synaptic connectivity and neuronal function of phasmid sensory neurons. Sex-specificity of DMD-4 function is conferred by a novel mode of *For correspondence: posttranslational regulation that involves sex-specific protein stabilization through ubiquitin binding [email protected] to a phylogenetically conserved but previously unstudied protein domain, the DMA domain. A Competing interest: See human DMRT homolog of DMD-4 is controlled in a similar manner, indicating that our findings may page 25 have implications for the control of sexual differentiation in other animals as well.
    [Show full text]
  • Roles of Wrky Proteins in Mediating the Crosstalk of Hormone Signaling Pathways: an Approach Integrating Bioinformatics and Experimental Biology
    UNLV Retrospective Theses & Dissertations 1-1-2006 Roles of Wrky proteins in mediating the crosstalk of hormone signaling pathways: An approach integrating bioinformatics and experimental biology Zhen Xie University of Nevada, Las Vegas Follow this and additional works at: https://digitalscholarship.unlv.edu/rtds Repository Citation Xie, Zhen, "Roles of Wrky proteins in mediating the crosstalk of hormone signaling pathways: An approach integrating bioinformatics and experimental biology" (2006). UNLV Retrospective Theses & Dissertations. 2711. http://dx.doi.org/10.25669/0cw1-sipg This Dissertation is protected by copyright and/or related rights. It has been brought to you by Digital Scholarship@UNLV with permission from the rights-holder(s). You are free to use this Dissertation in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s) directly, unless additional rights are indicated by a Creative Commons license in the record and/or on the work itself. This Dissertation has been accepted for inclusion in UNLV Retrospective Theses & Dissertations by an authorized administrator of Digital Scholarship@UNLV. For more information, please contact [email protected]. ROLES OF WRKY PROTEINS IN MEDIATING THE CROSSTALK OF HORMONE SIGNALING PATHWAYS: AN APPROACH INTEGRATING BIOINFORMATICS AND EXPERIMENTAL BIOLOGY by Zhen Xie Bachelor of Sciences Shandong Agricultural University 1998 Master of Sciences Shandong Agricultural University 2001 A dissertation submitted in partial fulfillment of the requirements for the Doctor of Philosophy Degree in Biological Sciences Department of Biological Sciences College of Sciences Graduate College University of Nevada, Las Vegas December 2006 Reproduced with permission of the copyright owner.
    [Show full text]
  • Cellular Roles of PKN1
    Cellular Roles of PKN1 Neil Torbett This thesis is submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy University College University of London August 2003 London Research Institute Cancer Research UK 44 Lincoln’s Inn Fields London W C2A 3PX ProQuest Number: U642486 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest. ProQuest U642486 Published by ProQuest LLC(2015). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States Code. Microform Edition © ProQuest LLC. ProQuest LLC 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106-1346 A b s t r a c t Protein Kinase Novel 1 (PKN1), which in part resembles yeast PKCs, has been shown to be under the control of Rho GTPases and 3-Phosphoinositide Dependent Kinase 1. Initial studies tested the hypothesis that Rho-PKNI inputs control PKB phosphorylation. Despite a demonstrable intervention in Rho function, no evidence was obtained that indicated a role for Rho-PKN1 in PKB control. In seeking a cellular role for PKN1, it was found that GPP tagged PKN1 has the ability to translocate in a reversible manner to a vesicular compartment following hyperosmotic stress. PKN1 kinase activity is not necessary for this translocation and in fact the PKN inhibitor HA1077 is also shown to induce PKN1 vesicle accumulation.
    [Show full text]
  • Identification and Characterization of the Doublesex Gene of Nasonia Oliveira, D.C.S.G.; Werren, J.H.; Verhulst, E.C.; Giebel, J
    University of Groningen Identification and characterization of the doublesex gene of Nasonia Oliveira, D.C.S.G.; Werren, J.H.; Verhulst, E.C.; Giebel, J. D.; Kamping, A.; Beukeboom, L. W.; van de Zande, L. Published in: Insect Molecular Biology DOI: 10.1111/j.1365-2583.2009.00874.x IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2009 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Oliveira, D. C. S. G., Werren, J. H., Verhulst, E. C., Giebel, J. D., Kamping, A., Beukeboom, L. W., & van de Zande, L. (2009). Identification and characterization of the doublesex gene of Nasonia. Insect Molecular Biology, 18(3), 315-324. https://doi.org/10.1111/j.1365-2583.2009.00874.x Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license. More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne- amendment. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
    [Show full text]
  • Sexual Dimorphism in Diverse Metazoans Is Regulated by a Novel Class of Intertwined Zinc Fingers
    Downloaded from genesdev.cshlp.org on October 4, 2021 - Published by Cold Spring Harbor Laboratory Press Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers Lingyang Zhu,1,4 Jill Wilken,2 Nelson B. Phillips,3 Umadevi Narendra,3 Ging Chan,1 Stephen M. Stratton,2 Stephen B. Kent,2 and Michael A. Weiss1,3–5 1Center for Molecular Oncology, Departments of Biochemistry & Molecular Biology and Chemistry, The University of Chicago, Chicago, Illinois 60637-5419 USA; 2Gryphon Sciences, South San Francisco, California 94080 USA; 3Department of Biochemistry, Case Western Reserve School of Medicine, Cleveland, Ohio 44106-4935 USA Sex determination is regulated by diverse pathways. Although upstream signals vary, a cysteine-rich DNA-binding domain (the DM motif) is conserved within downstream transcription factors of Drosophila melanogaster (Doublesex) and Caenorhabditis elegans (MAB-3). Vertebrate DM genes have likewise been identified and, remarkably, are associated with human sex reversal (46, XY gonadal dysgenesis). Here we demonstrate that the structure of the Doublesex domain contains a novel zinc module and disordered tail. The module consists of intertwined CCHC and HCCC Zn2+-binding sites; the tail functions as a nascent recognition ␣-helix. Mutations in either Zn2+-binding site or tail can lead to an intersex phenotype. The motif binds in the DNA minor groove without sharp DNA bending. These molecular features, unusual among zinc fingers and zinc modules, underlie the organization of a Drosophila enhancer that integrates sex- and tissue-specific signals. The structure provides a foundation for analysis of DM mutations affecting sexual dimorphism and courtship behavior.
    [Show full text]
  • MIND Bomb 2 Prevents RIPK1 Kinase Activity-Dependent and -Independent Apoptosis Through Ubiquitylation of Cflipl
    ARTICLE https://doi.org/10.1038/s42003-020-01603-y OPEN MIND bomb 2 prevents RIPK1 kinase activity-dependent and -independent apoptosis through ubiquitylation of cFLIPL Osamu Nakabayashi 1, Hirotaka Takahashi2, Kenta Moriwaki1, Sachiko Komazawa-Sakon1, Fumiaki Ohtake3, 1234567890():,; Shin Murai 1, Yuichi Tsuchiya1, Yuki Koyahara1, Yasushi Saeki 4, Yukiko Yoshida4, Soh Yamazaki1, ✉ Fuminori Tokunaga 5, Tatsuya Sawasaki 2 & Hiroyasu Nakano 1 Mind bomb 2 (MIB2) is an E3 ligase involved in Notch signalling and attenuates TNF-induced apoptosis through ubiquitylation of receptor-interacting protein kinase 1 (RIPK1) and cylin- dromatosis. Here we show that MIB2 bound and conjugated K48– and K63–linked poly- ubiquitin chains to a long-form of cellular FLICE-inhibitory protein (cFLIPL), a catalytically inactive homologue of caspase 8. Deletion of MIB2 did not impair the TNF-induced complex I formation that mediates NF-κB activation but significantly enhanced formation of cytosolic death-inducing signalling complex II. TNF-induced RIPK1 Ser166 phosphorylation, a hallmark of RIPK1 death-inducing activity, was enhanced in MIB2 knockout cells, as was RIPK1 kinase activity-dependent and -independent apoptosis. Moreover, RIPK1 kinase activity-independent apoptosis was induced in cells expressing cFLIPL mutants lacking MIB2-dependent ubiqui- tylation. Together, these results suggest that MIB2 suppresses both RIPK1 kinase activity- dependent and -independent apoptosis, through suppression of RIPK1 kinase activity and ubiquitylation of cFLIPL, respectively. 1 Department of Biochemistry, Toho University School of Medicine, 5-21-16 Omori-Nishi, Ota-ku, Tokyo 143-8540, Japan. 2 Division of Cell-Free Sciences, Proteo-Science Center (PROS), 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan.
    [Show full text]
  • Wo 2008/022335 A2
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date (10) International Publication Number 21 February 2008 (21.02.2008) PCT WO 2008/022335 A2 (51) International Patent Classification: Pemburton Avenue, #806, Toronto, Ontario M2M 4K8 C12Q 1/68 (2006.01) (CA). (21) International Application Number: (74) Agents: MYERS, Louis et al.; Fish & Richardson P.C., PCT/US2007/076248 P.O. Box 1022, Minneapolis, Minnesota 55440-1022 (US). (22) International Filing Date: 17 August 2007 (17.08.2007) (81) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of national protection available): AE, AG, AL, AM, AT,AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, (26) Publication Language: English CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, (30) Priority Data: ES, FT, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, 60/838,662 18 August 2006 (18.08.2006) US IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, 60/845,564 19 September 2006 (19.09.2006) US LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, (71) Applicants (for all designated States except US): THE PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, BRIGHAM AND WOMEN'S HOSPITAL, INC. TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, [US/US]; 75 Francis Street, Boston, Massachusetts 021 15 ZM, ZW (US).
    [Show full text]
  • Dmrt1, a Gene Related to Worm and Fly Sexual Regulators, Is Required for Mammalian Testis Differentiation
    Downloaded from genesdev.cshlp.org on October 7, 2021 - Published by Cold Spring Harbor Laboratory Press Dmrt1, a gene related to worm and fly sexual regulators, is required for mammalian testis differentiation Christopher S. Raymond,1 Mark W. Murphy,2 M. Gerard O’Sullivan,3 Vivian J. Bardwell,2,4 and David Zarkower1,2,5 1Graduate Program in Biochemistry, Molecular Biology and Biophysics, 2Department of Genetics, Cell Biology, and Development, 3Department of Veterinary Medicine and Pathology, 4Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA. The only molecular similarity in sex determination found so far among phyla is between the Drosophila doublesex (dsx) and Caenorhabditis elegans mab-3 genes. dsx and mab-3 contain a zinc finger-like DNA-binding motif called the DM domain, perform several related regulatory functions, and are at least partially interchangeable in vivo. A DM domain gene called Dmrt1 has been implicated in male gonad development in a variety of vertebrates, on the basis of embryonic expression and chromosomal location. Such evidence is highly suggestive of a conserved role(s) for Dmrt1 in vertebrate sexual development, but there has been no functional analysis of this gene in any species. Here we show that murine Dmrt1 is essential for postnatal testis differentiation, with mutant phenotypes similar to those caused by human chromosome 9p deletions that remove the gene. As in the case of 9p deletions, Dmrt1 is dispensable for ovary development in the mouse. Thus, as in invertebrates, a DM domain gene regulates vertebrate male development. [Key Words: DM domain; Dmrt1; sex determination; doublesex; mab-3] Received July 15, 2000; revised version accepted September 6, 2000.
    [Show full text]
  • 253 References
    References Aanes, H., C. L. Winata, C. H. Lin, J. P. Chen, K. G. Srinivasan, S. G. Lee, A. Y. Lim, H. S. Hajan, P. Collas, G. Bourque, Z. Gong, V. Korzh, P. Alestrom and S. Mathavan (2011). "Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition." Genome Res 21(8): 1328-1338. Aberdam, D., T. Virolle and P. Simon-Assmann (2000). "Transcriptional regulation of laminin gene expression." Microsc Res Tech 51(3): 228-237. Abrass, C. K. and K. M. Hansen (2010). "Insulin-like growth factor-binding protein-5-induced laminin gamma1 transcription requires filamin A." J Biol Chem 285(17): 12925-12934. Ackley, B. D., S. H. Kang, J. R. Crew, C. Suh, Y. Jin and J. M. Kramer (2003). "The basement membrane components nidogen and type XVIII collagen regulate organization of neuromuscular junctions in Caenorhabditis elegans." J Neurosci 23(9): 3577-3587. Airenne, T., H. Haakana, K. Sainio, T. Kallunki, P. Kallunki, H. Sariola and K. Tryggvason (1996). "Structure of the human laminin gamma 2 chain gene (LAMC2): alternative splicing with different tissue distribution of two transcripts." Genomics 32(1): 54-64. Alexandre, C., A. Jacinto and P. W. Ingham (1996). "Transcriptional activation of hedgehog target genes in Drosophila is mediated directly by the cubitus interruptus protein, a member of the GLI family of zinc finger DNA-binding proteins." Genes Dev 10(16): 2003-2013. Allamand, V., Y. Sunada, M. A. Salih, V. Straub, C. O. Ozo, M. H. Al-Turaiki, M. Akbar, T. Kolo, H. Colognato, X. Zhang, L. M. Sorokin, P.
    [Show full text]