DOCSLIB.ORG

Pharmacognosy and Phytotherapy Volume 6 Number 5, June 2014 ISSN 2141-2502

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Pharmacognosy and Phytotherapy Volume 6 Number 5, June 2014 ISSN 2141-2502 Journal of Pharmacognosy and Phytotherapy Volume 6 Number 5, June 2014 ISSN 2141-2502 ABOUT JPP The Journal of Pharmacognosy and Phytotherapy (JPP) is published monthly (one volume per year) by Academic Journals. The Journal of Pharmacognosy and Phytotherapy (JPP) is an open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as ethnobotany, phytochemistry, ethnopharmacology, zoopharmacognosy, medical anthropology etc. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published shortly after acceptance. All articles published in JPP are peer-reviewed. Submission of Manuscript Submit manuscripts as e-mail attachment to the Editorial Office at: [email protected]. A manuscript number will be mailed to the corresponding author shortly after submission. The Journal of Pharmacognosy and Phytotherapy (JPP) will only accept manuscripts submitted as e-mail attachments. Please read the Instructions for Authors before submitting your manuscript. The manuscript files should be given the last name of the first author. Editors Dr. (Mrs) Banasri Hazra Dr. Maryam Sarwat Research Scientist (U.G.C.) C/O A.M. Khan, House No. 195 Department of Pharmaceutical Technology Jadavpur University Dr. Yong-Jiang Xu Calcutta - 700032 Saw Swee Hock School of Public Health, India National University of Singapore, Singapore. Prof. Dr. Adeolu Alex Adedapo Dr. Yuanxiong Deng Department of Veterinary Physiology, Dept of Pharmaceutical Science Biochemistry and Pharmacology School of Medicine University of Ibadan, Nigeria Hunan Normal University Tongzipo Road 371, Changsha 410013, Dr. Joana S. Amaral Hunan China Campus de Sta Apolónia, Ap. 1134, 5301-857 Bragança, Portugal Prof. Maha Aboul Ela Beirut Arab University, Faculty of Pharmacy, Beirut Dr. Asad Ullah Khan Campus Interdisciplinary Biotechnology UNIT Aligarh Muslim University, Dr. S. RAJESWARA REDDY India Assistant Professor, Division of Animal Biotechnology Department of Biotechnology, School of Herbal Dr. Sunday Ene-ojo Atawodi Studies and Naturo Sciences, Biochemistry Department Dravidian University, Kuppam – 517 425, A.P. Ahmadu Bello University India Zaria, Nigeria Dr. Mekhfi Hassane Prof. Fukai Bao University Mohammed the First, Faculty of Sciences, Department of Microbiology and Immunology, Department of biology, Oujda, Morocco Kunming Medical College Morocco China Dr. Ilkay Erdogan Orhan Dr. Bhaskar C Behera Faculty of Pharmacy, Gazi University, Agharkar Research Institute Ankara, Turkey Dept. of Secience &Technology, Turkey Plant Science Division India Dr. Arun Kumar Tripathi Central Insttute of Medicinal and Aromatic Plants Prof. R. Balakrishna Bhat P.O. CIMAP, LUCKNOW-226015, Walter Sisulu University India Department of Botany Mthatha, South Africa Dr. Wesley Lyeverton Correia Ribeiro Universidade Estadual do Ceará, Faculdade Dr. Mohammad Nazrul Islam Bhuiyan de Veterinária/Laboratório de Doenças BCSIR Laboratories; Parasitárias Av. Paranjana, 1700 Chittagong cantonment; Itaperi - Fortaleza Chittagong-4220; 60740-903, CE - Brazil Bangladesh Dr. Baojun Bruce Xu Dr. Adibe Maxwell Ogochukwu Beijing Normal University-Hong Kong Baptist Clinical Pharmacy and Pharmacy Management, University United International College Zhuhai, Faculty of Pharmaceutical Sciences, Guangdong Province, University of Nigeria, Nsukka China Enugu state, Nigeria. Dr. Hamad H. Issa Department of Physical Sciences, Dr. Odukoya, Olukemi Abiodun School of natural Sciences, Department of Pharmacognosy, The University of Dodoma, Faculty of Pharmacy Tanzania University of Lagos. Nigeria. Dr. Gagan Deep Department of Pharmaceutical Sciences Dr. Qinxue Richard Ding School of Pharmacy, Medical Center at Stanford University, University of Colorado Denver, Palo Alto, Colorado, USA USA Dr. Sulejman Redžic Dr. Fengguo Xu Faculty of Science of the University of Sarajevo Dept of Epidemiology and Public Health, 33-35 Zmaja od Bosne St., Yong Loo Lin School of Medicine, Sarajevo, National University of Singapore, Bosnia and Herzegovina Singapore Dr. Michal Tomczyk Dr. Haitao Lv Medical University of Bialystok, Medicine and Endocrinology, Faculty of Pharmacy, Albert Einstein College of Meidicine, Department of Pharmacognosy, Yeshiva University, Poland USA Dr. Ugur Çakilcioglu Hassane MEKHFI Firat University, University Mohammed the First, Faculty of Science and Arts, Faculty of Sciences, Department of Biology, Department of biology, Elazig Laboratory of Physiology and Ethnopharmacology, Turkey Morocco Dr. Subhash C. Mandal Prof. Samson Sibanda Division of Pharmacognosy National University of Science and Technology Pharmacognosy and Phytotherapy Research Cnr Gwanda Road/Cecil Avenue, Laboratory, Ascot, Bulawayo, Department of Pharmaceutical Technology, Zimbabwe Jadavpur University, India. Journal of Pharmacognosy and Phytotherapy Table of Contents: Volume 6 Number 5 June 2014 ARTICLES Research Articles Risk of liver damage, reduced serum T3 and TSH, reduced placental and litter birth weights are some of the outcomes of Hybanthus enneaspermus infusion to pregnant rats 54 Awobajo F. O., Iranloye B. O., Olatunji-Bello I. I. and Adegoke O. A. African medicinal plant derived products as therapeutic arsenals against multidrug resistant microorganisms 59 Assob Jules Clement Nguedia and Nsagha Dickson Shey Vol. 6(5), pp. 54-58, June 2014 DOI: 10.5897/JPP2014.0310 Article Number: 7E1D00945350 Journal of Pharmacognosy and ISSN 2141-2502 Copyright © 2014 Phytotherapy Author(s) retain the copyright of this article http://www.academicjournals.org/JPP Full Length Research Paper Risk of liver damage, reduced serum T3 and TSH, reduced placental and litter birth weights are some of the outcomes of Hybanthus enneaspermus infusion to pregnant rats Awobajo F. O., Iranloye B. O., Olatunji-Bello I. I. and Adegoke O. A. Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Nigeria. Received 27 Jan, 2014: Accepted 30 May, 2014 Hybanthus enneaspermus is used by traditional birth attendants (TBAs) in southwest Nigeria in antenatal care. We examined the effects of oral administration of aqueous leaf extract of H. enneaspermus (HEaq) on liver weight and assay, maternal-thyroid hormones, foetal growth and litters birth weights in Sprague-Dawley (SD) rats. Twelve pregnant SD rats were used for this study. Control group received distilled water while the test group received 2 g/kg body weight of HEaq orally throughout the period of pregnancy. On day 19 of pregnancy, animals were sacrificed by CO2 asphyxiation and cervical dislocation. Blood samples were withdrawn into sterile tubes and centrifuged to obtain serum that was used for assay. Liver hormogenate was used for liver function test. The following were assayed; alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase (ALT), thyroid hormone, thyrotrophin and TSH in both control and treated rats. Some rats were allowed to carry their pregnancy to term. All rules guiding animal care and humane handling methods of laboratory animals in animal experimentation were followed. The results of the study showed a significant increase in serum and liver homogenate level of biomarkers of liver injury (ALP, ALT and AST). The serum T3 and TSH were significantly lowered in all extract treated rats compared to control, while T4 and thyrotrophin were not significantly altered. A significant reduction in placenta and foetal birth weights were recorded in the extract treated group. Oral administration of HEaq during pregnancy adversely affected maternal thyroid hormones, placental and foetal development. HEaq administration to pregnant rats also precipitated increased biomarkers of liver damage. Key words: Hybanthus enneaspermus, pregnancy outcome, thyroid hormone, litters birth weight, liver function test. INTRODUCTION The activities of the traditional birth attendants (TBAs) are enneaspermus is one of the most important medicinal still common in Nigeria society where they attend to > plants used in southwest Nigeria by the TBAs (Awobajo 50% of deliveries (Oruamabo, 2007). One of the tools et al., 2013). The TBAs claimed that administration of the used by the TBAs is medicinal plant. Hybanthus concoction of the leaf extract of the H. enneaspermus Awobajo et al. 55 (HEaq) plant invigorate the pregnant woman, boost the following assays were carried out; alanine aminotransferase (ALT), haematological parameters and make delivery easy and aspartate aminotransferase (AST), alkaline phosphatase (ALP) and safe (Awobajo et al., 2013). However, these claims are thyroid hormones assay. AST, ALT, ALP was measured using commercially available kits ELISA kit and a UV-spectrophotometer not scientifically verified. Several other medicinal plants (Hitachi 736-60, Japan). Hormonal assay was carried out with have been shown to possess some advantages while Randox assay kit, (UK) according to manufacturer specification. many others have deleterious effects on body system The liver was dissected out over ice pack, homogenized in phos- (Dahanukar et al., 2000). phate buffer after which it was centrifuged at 1500 g for 15 min. The H. enneaspermus (Linn) F. Mull commonly called supernatant from liver hormogenate and serum from blood samples were stored frozen at -8°C until used for the assays. “Abiwere” in Yoruba land, south west Nigeria is a shrub that belongs to the Violaceae family. We have earlier reported on the identification, extraction,
Load more

Recommended publications
  • List of Candidates Who Have Not Submitted Their Neet Ug 2017 Roll Number
    LIST OF CANDIDATES WHO HAVE NOT SUBMITTED THEIR NEET UG 2017 ROLL NUMBER Sl. Application Candidate's Name Father's Name Mother's Name DOB No. No. 1 100249 SACHIN KUMAR PAWAN KUMAR ANITA SHARMA 15.Nov.93 2 100808 ANUSHEEL NAGAR OMBIR SINGH GEETA DEVI 07/Oct/1998 3 101222 AKSHITA DAAGAR SUSHIL KUMAR SEEMA 24/May/1999 4 101469 ANKITA YADAV SANJAY YADAV CHANCHAL YADAV 31/Dec/1999 5 101593 ZEWA NAWAB KHAN NOOR JAHAN 10/Feb/1999 6 101814 SHAGUN SHARMA GAGAN SHARMA RACHNA SHARMA 13/Oct/1998 7 102087 MOHD FAISAL SHAHABUDDIN JANNATUL FIRDOUS 14/Aug/1998 8 102121 SNEHA SOBODH KUMAR RAJENDRI 20/Jul/1998 9 102256 ABUZAR MD SHAHZAMAN SHABNAM SABA 25/Mar/1999 10 102397 ANJALI DESWAL JAGBIR SINGH RAJ RANI 05/Sep/1998 11 102402 RASMEET KAUR SURINDER PAL SINGH GURVINDER KAUR 11/Sep/1997 12 102474 ABU TALHA MD SHAHZAMAN SHABNAM SABA 25/Mar/1999 13 102515 SHIVANI SHARMA RAKESH KUMAR SHARMA KRISHNA SHARMA 01/Aug/2000 14 102529 POONAM SHARMA GOVIND SHARMA RAJESH SHARMA 04/Nov/1998 15 102532 SHAISTA BANO MD SHAHZAMAN SHABNAM SABA 09/Nov/1997 KARUNA KUMARI 16 102711 ANUSHKA RAJ SINGH RAJ KUMAR SINGH 12/Mar/2000 SINGH 17 102841 AAMIR SUHAIL MALIK NASIMUDDIN SHANNO 26/May/1996 18 102971 ABLE MOGHA MANOJ KUMAR MOGHA SARITA MOGHA 19/Aug/1998 19 103053 HARSHITA AGRAWAL MOHIT AGRAWAL MEERA AGRAWAL 07/Sep/1999 20 103245 HIMANSHI GUPTA VIJAY KUMAR GUPTA INDU GUPTA 09/May/2000 NGULLIENTHANG 21 103428 PAOKHUP HAOKIP VANKHOMOI HAOKIP 03/Oct/1998 HAOKIP 22 103433 APOORVA SINHA GAUTAM BIRENDRA ALPANA DEVA 12/Sep/1999 23 103518 TANYA GUPTA RAJIV GUPTA VANDANA GUPTA 29/Apr/1999
    [Show full text]
  • Enrolment List Dated 20.10.2020
    Enrolment List dated 20.10.2020 S.No. D/ EnNo Year Date Month Name Father Name Documents to be submitted 1 D/ 1 /2020 02nd January, Gaurav Dixit Tejpal Singh Attendance Certificate 2 D/ 2 /2020 02nd January, Mohit Sharma U.K. Sharma 3 D/ 3 /2020 06th January, Shailendra Sharma S.C. Sharma 4 D/ 4 /2020 06th January, Kasiraman K. Kannuchamy K. 5 D/ 5 /2020 06th January, Saurabh Kumar Suman Ramakant Jha Grad. & PG Degree 6 D/ 6 /2020 06th January, Gaurav Sharma K.K. Sharma 7 D/ 7 /2020 06th January, Jai Prakash Agrawal Tek Chand Agrawal FIR Pending 8 D/ 8 /2020 07th January, Renuka Madan Balram Krishan Madan 9 D/ 9 /2020 07th January, Arokia Arputha Raj T. Thomas Org. LLB. Attendance Certificate 10 D/ 10 /2020 07th January, Anandakumar P. O. Paldurai 11 D/ 11 /2020 08th January, Anurag Kumar Late B.L. Grover 12 D/ 12 /2020 08th January, Atika Alvi Late Umar Mohd. Alvi 13 D/ 13 /2020 08th January, Shahrukh Kirmani Shariq Mahmood 14 D/ 14 /2020 08th January, Shreya Bali Sanjay Bali 15 D/ 15 /2020 09th January, Ashok Kumar Dhanpal Grad. Degree 16 D/ 16 /2020 09th January, Sachin Bhati Mansingh Bhati 17 D/ 17 /2020 09th January, Jaskiran Kaur Satwinder Singh 18 D/ 18 /2020 09th January, Hitain Bajaj Sunil Bajaj 19 D/ 19 /2020 09th January, Shikha Shukla Ravitosh Shukla Org. LLB. Attendace Certificate 20 D/ 20 /2020 10th Janaury, Paramjeet Singh Avtar Singh 21 D/ 21 /2020 10th Janaury, Kalimuthu M. K. Murugan 22 D/ 22 /2020 13th January, Raju N.
    [Show full text]
  • Doctor of Philosophy
    Nanoparticle induced suppression of microbial biofilm THESIS SUBMITTED FOR THE AWARD OF THE DEGREE OF Doctor of Philosophy IN BIOTECHNOLOGY BY Shatavari Kulshrestha UNDER THE SUPERVISION OF Dr. ASAD ULLAH KHAN INTERDISCIPLINARY BIOTECHNOLOGY UNIT ALIGARH MUSLIM UNIVERSITY ALIGARH (INDIA) 2015 Certificate This is to certify that the thesis entitled “Nanoparticle induced suppression of microbial biofilm” herewith submitted by Shatavari Kulshrestha, in fulfilment of the requirements for the degree of Doctor of philosophy in Biotechnology of the Aligarh Muslim University, is an authentic record of the research work carried out by her under my supervision and guidance and that no part, thereof, has been presented before for any other degree. Prof. Asad Ullah Khan (Supervisor) Declaration I, hereby, declare that the thesis entitled “Nanoparticle induced suppression of microbial biofilm” is free from any sort of plagiarism. (Shatavari Kulshrestha) Declaration I, hereby, declare that the thesis entitled “Nanoparticle induced suppression of microbial biofilm” embodies the work carried out by me. (Shatavari Kulshrestha) COURSE WORK/COMPREHENSIVE EXAMINATION/ PRE- SUBMISSION SEMINAR COMPLETION CERTIFICATE This is to certify that Ms. Shatavari Kulshrestha, Interdisciplinary Biotechnology Unit, has satisfactorily completed the Course work/Comprehensive examination/Pre-submission seminar requirement which is a part of her Ph.D. programme. Date: ……………… (Signature of the Chairman) Acknowledgement ACKNOWLEDGEMENT All praises to the God, the almighty for providing me this opportunity and granting me the capability to proceed successfully ….. I take this opportunity to express my deep and sincere gratitude to my supervisor Dr. Asad U Khan, Professor, Interdisciplinary biotechnology unit AMU, for giving me the opportunity to do research and providing invaluable guidance throughout this study.
    [Show full text]
  • Original Article Amplification of Meca Gene in Multi-Drug Resistant
    Original Article Amplification of mecA gene in multi-drug resistant Staphylococcus aureus strains from hospital personnel Asad U Khan,1 Ayesha Sultan,1 Anju Tyagi,2 Shazia Zahoor,3 Mohd Akram,1 Sukhminderjit Kaur,3 Mohd Shahid,2 Chetana V Vaishnavi 1 1Interdisciplinary Biotechnology Unit, Aligarh Muslim University Aligarh 202002 India; 2Microbiology Department JNMC, AMU, Aligarh, India; 3Gastroenterology Department, PGIMER, Chandigarh, India. Abstract Background: Antibiotic resistance is common among bacterial pathogens associated with both community acquired and nosocomial infections. In view of the present problem of drug resistance we investigated the prevalence of methicillin resistant Staphylococcus aureus (MRSA) and amplified the mecA gene in the isolates from the hand swabs of the hospital personnel. Methodology: The nuc gene was amplified to characterize these isolates at species level. The S. aureus isolates were analyzed for their susceptibility to different classes of antibiotics using the disk diffusion method. The spot inoculation test was performed to detect methicillinase production in these isolates. Results: In the screened isolates of S. aureus , 14.2 and 15 kb of plasmids were present. These isolates showed pronounced resistance against β-lactam antibiotics including second- and third-generation cephalosporins, aminoglycosides, macrolides and fluoroquinolone. Some of the isolates included in this study were resistant to three or more antibiotics. Expression of methicillinase was detected by spot inoculation test, and a few of the isolates were found to produce methicillinase. Moreover, mecA gene was also amplified. Of 17 isolates only 7 showed presence of mecA gene. Conclusion: This study highlights the emerging trend of multiple drug resistance in S. aureus strains isolated from hospital personnel working in a premier hospital in North India.
    [Show full text]
  • Maulana Azad Library, Aligarh Muslim University
    MOLECULAR CHARACTERIZATION OF CARBAPENEMASE PRODUCING BACTERIAL STRAIN OF ENTEROBACTERIACEAE FAMILY THESIS SUBMITTED FOR THE AWARD OF THE DEGREE OF Doctor of Philosophy IN BIOTECHNOLOGY BY NAYEEM AHMAD UNDER THE SUPERVISION OF PROF. ASAD ULLAH KHAN MaulanaINTERDISCIPLINARY Azad Library, BIAligarhOTECHNOLOGY Muslim UNITUniversity ALIGARH MUSLIM UNIVERSITY ALIGARH - 202002 (INDIA) 2019 Certificate This is to certify that the thesis entitled “Molecular characterization of carbapenemase producing bacterial strain of enterobacteriaceae family” herewith submitted by Nayeem Ahmad, in fulfilment of the requirement for the degree of Doctor of Philosophy in Biotechnology of the Aligarh Muslim University, Aligarh, is an authentic record of the research work carried out by him under my supervision and guidance and that no part, thereof, has been presented before for any other degree. He has fulfilled all the prerequisites necessary for the submission of the Ph.D. thesis according to 2009 regulation of University Grants Commission, New Delhi. Date: (Prof. Asad U Khan) Maulana Azad Library, Aligarh Muslim (Ph.D. University Supervisor) Candidate DECLARATION I, hereby declare that thesis entitled “Molecular characterization of carbapenemase producing bacterial strain of enterobacteriaceae family” embodies the work carried out by me. Dated: (Nayeem Ahmad) Maulana Azad Library, Aligarh Muslim University COURSE WORK/COMPREHENSIVE EXAMINATION/PRE-SUBMISSION SEMINAR COMPLETION CERTIFICATE This is to certify that Mr. Nayeem Ahmad, Interdisciplinary Biotechnology Unit, has satisfactorily completed the Coursework/Comprehensive examination/Pre-submission seminar requirement which is part of his Ph.D. programme. MaulanaDate:…….. Azad Library, Aligarh Muslim (Signature ofUniversity Chairman) Contents LIST OF CONTENTS Page No. Acknowledgement i-iii List of Abbreviation iv-v List of Figures vi-viii List of Tables ix Abstract x-xiii 1-33 Chapter 1: Review of Literature 1.1.
    [Show full text]