DOCSLIB.ORG

Biomarkers and Metabolic Patterns in the Sediments of Evolving Glacial Lakes As a Proxy for Planetary Lake Exploration

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Biomarkers and Metabolic Patterns in the Sediments of Evolving Glacial Lakes As a Proxy for Planetary Lake Exploration ASTROBIOLOGY Volume 18, Number 5, 2018 ª Mary Ann Liebert, Inc. DOI: 10.1089/ast.2015.1342 Biomarkers and Metabolic Patterns in the Sediments of Evolving Glacial Lakes as a Proxy for Planetary Lake Exploration Vı´ctor Parro,1 Yolanda Blanco,1 Fernando Puente-Sa´nchez,1 Luis A. Rivas,1 Mercedes Moreno-Paz,1 Alex Echeverrı´a,2 Guillermo Chong-Dı´az,2 Cecilia Demergasso,2 and Nathalie A. Cabrol3 Abstract Oligotrophic glacial lakes in the Andes Mountains serve as models to study the effects of climate change on natural biological systems. The persistent high UV regime and evolution of the lake biota due to deglaciation make Andean lake ecosystems potential analogues in the search for life on other planetary bodies. Our objective was to identify microbial biomarkers and metabolic patterns that represent time points in the evolutionary history of Andean glacial lakes, as these may be used in long-term studies as microscale indicators of climate change processes. We investigated a variety of microbial markers in shallow sediments from Laguna Negra and Lo Encan˜ado lakes (Regio´n Metropolitana, Chile). An on-site immunoassay-based Life Detector Chip (LDChip) revealed the presence of sulfate-reducing bacteria, methanogenic archaea, and exopolymeric substances from Gammaproteobacteria. Bacterial and archaeal 16S rRNA gene sequences obtained from field samples confirmed the results from the immunoassays and also revealed the presence of Alpha-, Beta-, Gamma-, and Deltaproteo- bacteria, as well as cyanobacteria and methanogenic archaea. The complementary immunoassay and phylogenetic results indicate a rich microbial diversity with active sulfate reduction and methanogenic activities along the shoreline and in shallow sediments. Sulfate inputs from the surrounding volcanic terrains during deglaciation may explain the observed microbial biomarker and metabolic patterns, which differ with depth and between the two lakes. A switch from aerobic and heterotrophic metabolisms to anaerobic ones such as sulfate reduction and methanogenesis in the shallow shores likely reflects the natural evolution of the lake sediments due to deglaciation. Hydrodynamic deposition of sediments creates compartmentalization (e.g., sediments with different structure and composition surrounded by oligotrophic water) that favors metabolic transitions. Similar phenomena would be expected to occur on other planetary lakes, such as those of Titan, where watery niches fed by depositional events would be surrounded by a ‘‘sea’’ of hydrocarbons. Key Words: Glacier lakes—Sedimentation—Prokaryotic metabolisms and biomarkers—Deglaciation—Life detection—Planetary exploration. Astrobiology 18, 586–606. 1. Introduction of the world’s largest sources of glacial water, which includes mountain glaciers, ice, and snowfields that are receding rap- ith global temperature rising, ice worldwide re- idly (Haeberli et al., 2002; Le Quesne and Acun˜a, 2003; Downloaded by Consejo Superior De Investigaciones Cientificas CSIC from www.liebertpub.com at 07/22/18. For personal use only. Wtreats and thins. It is projected that many low-altitude Coudrain et al., 2005; Bradley et al., 2006; Rivera et al., 2007; glaciers could disappear within 20 years (IPCC, 2007). Long- Vuille et al., 2008; Le Quesne et al., 2009). Glacial lakes and term, multiproxy studies in regions between 33°S and 36°Sin their sediments are highly sensitive temporal markers of en- Chile and Argentina have shown a mean frontal retreat of vironmental variability, which in turn affects their biota. between -50 and -9my-1, thinning rates between 0.76 and Microbiological studies of oligotrophic Andean lakes have 0.56 m y-1, and a mean ice area reduction of 3% since 1955 shown that changes in the water column occur in association (Roig et al., 2000; Le Quesne and Acun˜a, 2003; Lara et al., with fluctuations in water transparency or turbidity during de- 2005; Vuille, 2006; Le Quesne et al., 2009). The IPCC (2007) glaciation (Modenutti et al., 2012). During early deglaciation lists the Central and Southern Andean countries as particu- (phase 1), the high silt content of water protects microorgan- larly vulnerable (Painter, 2007). Among those, Chile has one isms from the high UV that occurs at the higher altitudes of 1Department of Molecular Evolution, Centro de Astrobiologı´a (INTA-CSIC), Madrid, Spain. 2Centro de Biotecnologı´a ‘‘Profesor Alberto Ruiz,’’ Universidad Cato´lica del Norte, Antofagasta, Chile. 3The SETI Institute, Carl Sagan Center, Mountain View, California, and NASA Ames Research Center, Moffett Field, California, USA. 586 BIOMARKERS IN EVOLVING GLACIAL LAKES 587 glacial lakes. With time, however, as glaciers continue to re- past 10 years and has been implemented—along with the cede, the meltwater discharge and sediment load into the lakes Signs of Life Detector (SOLID) instrument—for in situ detec- decrease (phase 2), and the transparency and UV levels of the tion of biomarkers (Parro et al., 2008, 2011b). The LDChip has water column increase. A comparative study of six Andean been used for in situ detection of prokaryotes and biomarker ultra-oligotrophic lakes characterized by low-phosphorus profiling in different extreme environments that include the concentrations revealed that high photosynthetically active acidic iron-rich sediments of the Rı´o Tinto in Spain (Parro et al., radiation (PAR), UVA, and UVB forced planktonic organisms 2008, 2011c; Puente-Sa´nchez et al., 2014), subsurface sedi- into deeper layers of the water column (Callieri et al., 2007). In ments (down to 5 m depth) cored in the hypersaline Atacama contrast, the microbial diversity (i.e., picocyanobacterial as- Desert (Parro et al., 2011a; Ferna´ndez-Remolar et al., 2013), semblages) of such lakes may remain high, a finding attributed and the surface and permafrost (down to 4.2 m) sediments to habitat fragmentation generated by geographic barriers, drilled on Deception Island in Antarctica (Blanco et al., 2012). which resulted in rapid speciation (Caravati et al., 2010). The LDChip results reported here reveal a rich geochemistry in The combination of environmental and climate factors can Andean lakes that is capable of sustaining an active anaerobic also affect the biodiversity of microbial communities in gla- metabolism and broad microbial diversity. Such data also cial lake sediments. Leo´n et al. (2012) reported differences in provides a baseline for further monitoring to understand the the metabolic activity and structural and functional compo- evolution of deglaciation as recorded in glacial lake sediments. sition of bacterial communities between the sediments of three Patagonian Chilean oligotrophic lakes of quaternary 2. Geological Setting glacial origin. They attributed these differences to the unique geomorphological pattern of each lake due to both local (e.g., Laguna Negra and Lo Encan˜ado are located on the south slope volcanic activity) and global (climate change) disturbances. of the Echaurren glacier watershed in the Central Andes of Chile Such examples illustrate how the microbial communities (33.65S, 70.13W; Fig. 1). Monitoring these lakes over time that live in the water column and sediments of glacial lakes and would allow for characterization of their prokaryotic diversity, reservoirs reflect their environmental setting and geochemical physical processes, and spatiotemporal changes. The two lakes input. Although lower and more acidic precipitation, increased are part of a complex of freshwater resources in the Santiago area temperature, and snow melting that accompanies deglaciation that includes El Yeso lake to the east, which is damned (on its strongly affect the dissolved organic carbon concentration of southern end). Though Laguna Negra and Lo Encan˜ado were glacial lakes (Beniston et al., 1997; Sala et al., 2000; Wil- connected by a human-made overflow tunnel, the lake level of liamson et al., 2009), microbes ultimately control the carbon Laguna Negra has decreased enough that the two lakes have cycle of glacial lakes through metabolic processes, such as been isolated from each other for at least the past 5 years. fermentation of complex organic matter or the production of Both lakes are located in the same catchment area (up to methane (Wadham et al.,2008). 4600 m in elevation), yet they are fed by two distinct stream Oligotrophic water, characterized by low organic carbon systems and have contrasting physical characteristics: Laguna and microbial biomass, is a general feature of glacial Andean Negra is a large (6.1 · 1.7 km) and deep (276 m in 2013) lake lakes. Although some works have reported on the microbial located at 2700 m above sea level, whereas Lo Encan˜ado is content, photosynthetic activity, and geochemistry of the water a smaller, 980 · 635 m wide, and 45 m deep lake located at column of these lakes, very little is actually known about their 2492 m elevation. sediments, which may reveal key aspects of the evolution of The watershed area of the lakes is composed primarily of these important ecosystems with regard to ecology, water re- volcanic constructs and basalt and andesite deposits that sources (e.g., for human consumption), and the evolution of were covered by modern glacial deposits from the receding glacier lakes at planetary scales. Determining whether there are Echaurren glacier, which now lies above the lakes at 3500 m predictable microbial successions or key biomarkers that might elevation. Lahar deposits mark recent to modern interactions be indicative of the progressive evolution of glacial lake
Load more

Recommended publications
  • Prokaryotic Diversity and Biogeochemical Characteristics of Benthic Microbial Ecosystems at La Brava, a Hypersaline Lake at Salar De Atacama, Chile
    RESEARCH ARTICLE Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems at La Brava, a hypersaline lake at Salar de Atacama, Chile Maria Eugenia Farias1*, Maria Cecilia Rasuk1, Kimberley L. Gallagher4, Manuel Contreras2, Daniel Kurth1, Ana Beatriz Fernandez1, Daniel Poire 3, Fernando Novoa2, Pieter T. Visscher4,5 a1111111111 1 Laboratorio de Investigaciones MicrobioloÂgicas de Lagunas Andinas (LIMLA), Planta Piloto de Procesos Industriales MicrobioloÂgicos (PROIMI), CCT-TucumaÂn, CONICET, TucumaÂn, Argentina, 2 Centro de a1111111111 EcologõÂa Aplicada (CEA), NÄ uñoa, Santiago, Chile, 3 Centro de Investigaciones GeoloÂgicas, Universidad a1111111111 Nacional de La Plata-Conicet, La Plata, Argentina, 4 Department of Marine Sciences, University of a1111111111 Connecticut, Groton, Connecticut, United States of America, 5 Australian Centre for Astrobiology, University a1111111111 of New South Wales, Sydney, New South Wales, Australia * [email protected] OPEN ACCESS Abstract Citation: Farias ME, Rasuk MC, Gallagher KL, Contreras M, Kurth D, Fernandez AB, et al. (2017) Benthic microbial ecosystems of Laguna La Brava, Salar de Atacama, a high altitude hyper- Prokaryotic diversity and biogeochemical saline lake, were characterized in terms of bacterial and archaeal diversity, biogeochem- characteristics of benthic microbial ecosystems at istry, (including O2 and sulfide depth profiles and mineralogy), and physicochemical La Brava, a hypersaline lake at Salar de Atacama, characteristics. La Brava is one of several lakes in the Salar de Atacama where microbial Chile. PLoS ONE 12(11): e0186867. https://doi.org/ 10.1371/journal.pone.0186867 communities are growing in extreme conditions, including high salinity, high solar insolation, and high levels of metals such as lithium, arsenic, magnesium, and calcium. Evaporation Editor: Stefan J.
    [Show full text]
  • Late Holocene Climate Variability in South- Central Chile: a Lacustrine Record of Southern Westerly Wind Dynamics
    Late Holocene Climate Variability in South- Central Chile: a Lacustrine Record of Southern Westerly Wind Dynamics. Jonas Vandenberghe ACKNOWLEDGEMENTS First of all, I would like to thanks Prof. Dr. Marc De Batist for the support and supervision of my thesis and for providing this interesting subject as a master thesis project. He was always available for questions and added meaningful corrections and insights on more difficult topics. Special thanks goes to my advisor Willem Vandoorne for his endless support, corrections and answers to my questions in both the lab and during the writing of my thesis. Also I would like to thank other researchers at RCMG especially Maarten and Katrien for very useful explanations and discussions. I am very thankful for the help of all other personel who, directly or indirectly, contributed to this work. Prof. Dr. Eddy Keppens, Leen and Michael from the VUB for their efforts during the carbon and nitrogen isotopic analysis and Rieneke Gielens for her great help with the XRF core scanning at NIOZ. Finally I would like to thank my parents for their support and my girlfriend Celine for coping with me when I was under stress and for providing solutions to all kinds of problems. 1 SAMENVATTING In de laatste decennia was er een opmerkelijke stijging van onderzoek in klimaat reconstructies van de voorbije 1000 jaar. Deze reconstructies moeten een bijdrage leveren aan het begrijpen van de recente klimaatsveranderingen. Klimaatsreconstructies van het laatste millennium kunnen informatie geven over de significantie van deze stijgende trend, de oorzaak van de opwarming en de invloed ervan op andere omgeveingsmechanismes op Aarde.
    [Show full text]
  • Conserved Bacterial Genomes from Two Geographically Isolated Peritidal Stromatolite Formations Shed Light on Potential Functional Guilds
    Environmental Microbiology Reports (2021) 13(2), 126–137 doi:10.1111/1758-2229.12916 Brief Report Conserved bacterial genomes from two geographically isolated peritidal stromatolite formations shed light on potential functional guilds Samantha C. Waterworth,1 Eric W. Isemonger,2 oldest fossils of living organisms on Earth (Dupraz Evan R. Rees,1 Rosemary A. Dorrington2 and et al., 2009; Nutman et al., 2016, 2019). The study of Jason C. Kwan 1* extant stromatolite analogues may help to elucidate the 1Division of Pharmaceutical Sciences, University of biological mechanisms that led to the formation and evo- Wisconsin, Madison, WI, 53705. lution of their ancient ancestors. The biogenicity of stro- 2Department of Biochemistry and Microbiology, Rhodes matolites has been studied extensively in the hypersaline University, Grahamstown, South Africa. and marine formations of Shark Bay, Australia, and Exuma Cay, The Bahamas, respectively (Khodadad and Foster, 2012; Mobberley et al., 2015; Centeno Summary et al., 2016; Gleeson et al., 2016; Ruvindy et al., 2016; Stromatolites are complex microbial mats that form Warden et al., 2016; White et al., 2016; Babilonia lithified layers. Fossilized stromatolites are the oldest et al., 2018; Wong et al., 2018; Chen et al., 2020). The evidence of cellular life on Earth, dating back over presence of Archaea has been noted in several microbial 3.4 billion years. Modern stromatolites are relatively mats and stromatolite systems (Casaburi et al., 2016; rare but may provide clues about the function and Balci et al., 2018; Medina-Chávez et al., 2019; Chen evolution of their ancient counterparts. In this study, et al., 2020), particularly in the stromatolites of Shark we focus on peritidal stromatolites occurring at Cape Bay, where they are hypothesized to potentially fulfil the Recife and Schoenmakerskop on the southeastern role of nitrifiers and hydrogenotrophic methanogens South African coastline, the former being morpholog- (Wong et al., 2017).
    [Show full text]
  • A Multidisciplinary Evaluation of Spatial Heterogeneity in the Puquios of the Salar De Llamara, Atacama Desert, Northern Chile
    A Multidisciplinary Evaluation of Spatial Heterogeneity in the Puquios of the Salar de Llamara, Atacama Desert, Northern Chile R. Pamela Reid ( [email protected] ) University of Miami Amanda Oehlert University of Miami Erica Suosaari Smithsonian Institution Cecilia Demergasso Catholic University of the North Guillermo Chong Catholic University of the North Lorena Escudero Catholic University of the North Alan Piggot Bahamas Marine EcoCentre Ioan Lascu Smithsonian Institution Alvaro Palma FisioAqua Research Article Keywords: Atacama Desert, high UV radiation, wide temperature variation, salt ats (salars) Posted Date: February 1st, 2021 DOI: https://doi.org/10.21203/rs.3.rs-153999/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/27 Abstract The Atacama Desert, Central Andes of Northern Chile, is an extreme environment characterized by high UV radiation, wide temperature variation, minimum precipitation and is reputed as the driest desert in the world. Scarce lagoons associated with salt ats (salars) in this desert are the surface expression of shallow groundwater which serve as refugia for life, and often host microbial communities associated with evaporitic mineral deposition. Recent investigations of the Puquios of the Salar de Llamara in the Atacama Desert based on multidisciplinary eld studies provide unprecedented detail regarding the spatial heterogeneity of physical, chemical, and biological characteristics of such saline lake environments. Four main lagoons (‘Puquios’) and more than 400 smaller ponds, occur in an area less than 5 km2, are characterized by high variability in electrical conductivity, benthic and planktonic biota and microbiota, lagoon bottom type, and style of mineral deposition.
    [Show full text]
  • Organomineralization of Microbialites from Storr's Lake, San Salvador
    Organomineralization of Microbialites from Storr’s Lake, San Salvador Island, Bahamas: Calcium Stable Isotope Analysis using TIMS and a 42Ca-43Ca double spike Thesis Presented in Partial Fulfillment of the Requirements for the Degree Master of Science in the Graduate School of The Ohio State University By Brittan Valhalla Wogsland, B.A. Graduate Program in Earth Sciences The Ohio State University 2020 Thesis Committee Elizabeth Griffith, Advisor Matthew Saltzman, Committee Member Thomas Darrah, Committee Member Copyrighted by Brittan Valhalla Wogsland 2020 2 Abstract The isotopic composition and mineralogy of modern microbialites provides us with tools useful for interpreting the formation processes and environments of ancient microbialites. Growing in the hypersaline and turbid Storr’s Lake on San Salvador Island in The Bahamas today are microbialites with low levels of photosynthesis and high levels of sulfate reduction-in contrast to many of their modern counterparts. Living planktonic, motile microorganisms and suspended algal and bacterial debris create the high turbidity of the shallow lake (<2 m) and rapidly attenuate sunlight in the water column. Within Storr’s Lake microbial metabolisms induce precipitation of carbonate within microenvironments of the microbial mats. Both high-Mg calcite (HMC) and aragonite are found within a majority of the microbialites measured leading to the hypothesis that the organomineralization process involves a step where HMC transforms to aragonite. Mineralogy and elemental analysis of a wide sampling of microbialites was undertaken to understand the extent of aragonite within Storr’s Lake microbialites. It was found that aragonite occurs at water depths greater than 40 cm within the lake and was present in all but one microbialite measured in this study.
    [Show full text]
  • Download Preprint
    1 A re-examination of the mechanism of whiting events: A new role 2 for diatoms in Fayetteville Green Lake (New York, USA) 3 Short running title: Re-examining whiting events in Fayetteville Green Lake 4 Authors and affiliations: 5 Chloe Stanton1, Julie Cosmidis1,2,*, and Lee R. Kump1,2 6 1Department of Geosciences, The Pennsylvania State University, University Park, PA 16802, 7 USA 8 2Earth and Environmental Systems Institute, The Pennsylvania State University, University Park, 9 PA 16802, USA 10 *Current address: Department of Earth Sciences, University of Oxford, Oxford OX1 03N, UK 11 12 Correspondence: 13 Julie Cosmidis, Department of Earth Sciences, University of Oxford, Oxford OX1 03N, UK. 14 Email: [email protected]. 15 Twitter : @JCosmidis 16 17 Key words: 18 Whitings, biomineralization, diatoms, calcite, micrite, ACC 19 20 21 This article is a non-peer reviewed preprint submitted to EarthArXiv. 22 It was submitted to Geobiology for peer review. 1 23 ABSTRACT 24 Whiting events – the episodic precipitation of fine-grained suspended calcium cabonates 25 in the water column – have been documented across a variety of marine and lacustrine 26 environments. Whitings likely are a major source of carbonate muds, a constituent of limestones, 27 and important archives for geochemical proxies of Earth history. While several biological and 28 physical mechanisms have been proposed to explain the onset of these precipitation events, no 29 consensus has been reached thus far. Fayetteville Green Lake (New York, USA), is a meromictic 30 lake that experiences annual whitings. Materials suspended in the water column collected through 31 the whiting season were characterized using scanning electron microscopy and scanning 32 transmission X-ray microscopy.
    [Show full text]
  • Las Quínoas Oncoids: a New Deposit of Microbialites in the Salar De Antofalla (Catamarca, Argentina)
    Andean Geology 48 (2): 281-302. May, 2021 Andean Geology doi: 10.5027/andgeoV48n2-3292 www.andeangeology.cl Las Quínoas oncoids: a new deposit of microbialites in the Salar de Antofalla (Catamarca, Argentina) *Patricio Guillermo Villafañe1, 3, Agustina Inés Lencina1, 2, Mariana Soria1, Luis Alberto Saona1, Fernando Javier Gómez4, Guido Ezequiel Alonso5, María Eugenia Farías1 1 Laboratorio de Investigaciones Microbiológicas en Lagunas Andinas (LIMLA), Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CONICET, Av. Belgrano y Pasaje Caseros S/N, 4000 San Miguel de Tucumán, Tucumán, Argentina. [email protected]; [email protected]; [email protected]; [email protected]; [email protected] 2 Centro de Investigaciones y Transferencias Catamarca (CITCa), CONICET, Prado 366, 4700 San Fernando del Valle de Catamarca, Catamarca, Argentina. 3 Instituto Superior de Correlación Geológica (INSUGEO), CONICET-UNT, Av. Presidente Perón S/N, 4107 Yerba Buena, Tucumán, Argentina. 4 Centro de Investigaciones en Ciencias de la Tierra (CICTERRA), CONICET-UNC (Universidad Nacional de Córdoba), Vélez Sarsfield 1611, Piso 2, Of. 4, Ciudad Universitaria, Córdoba, Argentina. [email protected] 5 Cátedra de Química General, Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán (UNT), Miguel Lillo 205, 4000 San Miguel de Tucumán, Tucumán, Argentina. [email protected] * Corresponding author: [email protected] ABSTRACT. The Salar de Antofalla (salt flat) is located in the Puna region of Catamarca, in northern Argentina. In this paper we report and provide the first descriptive data of Las Quínoas, a modern system of oncoids located in the western margin of the salt flat. Oncoids were studied by insitu logging, polished and thin sections analysis.
    [Show full text]
  • Electrical Conductivity As a Driver of Biological and Geological Spatial Heterogeneity in the Puquios, Salar De Llamara, Atacama Desert, Chile R
    www.nature.com/scientificreports OPEN Electrical conductivity as a driver of biological and geological spatial heterogeneity in the Puquios, Salar de Llamara, Atacama Desert, Chile R. P. Reid1,2*, A. M. Oehlert1,2, E. P. Suosaari1,2,3, C. Demergasso4, G. Chong5, L. V. Escudero4, A. M. Piggot2,6, I. Lascu3 & A. T. Palma7 Reputed to be the driest desert in the world, the Atacama Desert in the Central Andes of Northern Chile is an extreme environment with high UV radiation, wide temperature variation, and minimum precipitation. Scarce lagoons associated with salt fats (salars) in this desert are the surface expression of shallow groundwater; these ponds serve as refugia for life and often host microbial communities associated with evaporitic mineral deposition. Results based on multidisciplinary feld campaigns and associated laboratory examination of samples collected from the Puquios of the Salar de Llamara in the Atacama Desert during austral summer provide unprecedented detail regarding the spatial heterogeneity of physical, chemical, and biological characteristics of these salar environments. Four main lagoons (‘Puquios’) and more than 400 smaller ponds occur within an area less than 5 km2, and are characterized by high variability in electrical conductivity, benthic and planktonic biota, microbiota, lagoon bottom type, and style of mineral deposition. Results suggest that electrical conductivity is a driving force of system heterogeneity. Such spatial heterogeneity within the Puquios is likely to be expanded with temporal observations incorporating expected seasonal changes in electrical conductivity. The complexity of these Andean ecosystems may be key to their ability to persist in extreme environments at the edge of habitability.
    [Show full text]
  • Diurnal Variation in Bacterioplankton Composition and DNA Damage in the Microbial Community from an Andean Oligotrophic Lake
    Rev Argent Microbiol. 2014;46(4):358-362 REVISTA ARGENTINA DE MICROBIOLOGÍA www.elsevier.es/ram BRIEF REPORT Diurnal variation in bacterioplankton composition and DNA damage in the microbial community from an Andean oligotrophic lake María V. Fernández-Zenoffa,b,*, María C. Estéveza, María E. Faríasa a Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (LIMLA), Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CCT, CONICET, Tucumán, Argentina b Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina Recibido el 6 de junio de 2014; aceptado el 2 de octubre de 2014 KEYWORDS Abstract CPD; Laguna Azul is an oligotrophic lake situated at 4,560 m above sea level and subject to a DGGE; high level of solar radiation. Bacterioplankton community composition (BCC) was analysed Andean lakes; by denaturing gradient gel electrophoresis and the impact of solar ultraviolet radiation Ultraviolet radiation was assessed by measuring cyclobutane pyrimidine dimers (CPD). Furthermore, pure cultures of Acinetobacter johnsonii A2 and Rhodococcus sp. A5 were exposed simulta- neously and CPD accumulation was studied. Gel analyses generated a total of 7 sequences belonging to Alpha-proteobacteria (1 band), Beta-proteobacteria (1 band), Bacteroidetes (2 bands), Actinobacteria (1 band), and Firmicutes (1 band). DGGE proÀ les showed minimal changes in BCC and no CPD was detected even though a high level of damage was found in biodosimeters. A. johnsonii A2 showed low level of DNA damage while Rhodococcus sp. A5 exhibited high resistance since no CPD were detected under natural UV-B exposure, suggesting that the bacterial community is well adapted to this highly solar irradiated environment.
    [Show full text]
  • The Genomic Sequence of Exiguobacterium Chiriqhucha Str. N139 Reveals a Species That Thrives in Cold Waters and Extreme Environmental Conditions
    The genomic sequence of Exiguobacterium chiriqhucha str. N139 reveals a species that thrives in cold waters and extreme environmental conditions Ana Gutiérrez-Preciado1,8,*, Carlos Vargas-Chávez1,*, Mariana Reyes-Prieto1, Omar F. Ordoñez2, Diego Santos-García1,9, Tania Rosas-Pérez1, Jorge Valdivia-Anistro3,4, Eria A. Rebollar5, Andrés Saralegui6, Andrés Moya1, Enrique Merino7, María Eugenia Farías2, Amparo Latorre1 and Valeria Souza4 1 Unidad de Genética Evolutiva, Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universidad de Valencia, Calle Catedrático José Beltrán Martínez, Paterna, Valencia, Spain 2 Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas, Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Belgrano y Pasaje Caseros, San Miguel de Tucumán, Argentina 3 Carrera de Biología, Faculta de Estudios Superiores Zaragoza, UNAM, Mexico City, Mexico 4 Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México coyoacan, Mexico City, México 5 Department of Biology, James Madison University, Harrisonburg, VI, United States of America 6 Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México 7 Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México 8 Current affiliation: Ecologie Systématique Evolution, CNRS, AgroParisTech,
    [Show full text]
  • Meneses-Ortegón. Bacterioplancton En Lagunas Altoandinas De Boyacá, Colombia
    Meneses-Ortegón. Bacterioplancton en lagunas altoandinas de Boyacá, Colombia BACTERIOPLANCTON DE TRES LAGUNAS DE ALTA MONTAÑA TROPICAL ANDINA EN EL DEPARTAMENTO DE BOYACÁ, COLOMBIA LUZ ANDREA MENESES ORTEGÓN UNIVERSIDAD PEDAGÓGICA Y TECNOLÓGICA DE COLOMBIA FACULTAD DE CIENCIAS BÁSICAS MAESTRÍA EN CIENCIAS BIOLÓGICAS TUNJA 2013 1 Meneses-Ortegón. Bacterioplancton en lagunas altoandinas de Boyacá, Colombia BACTERIOPLANCTON DE TRES LAGUNAS DE ALTA MONTAÑA TROPICAL ANDINA EN EL DEPARTAMENTO DE BOYACÁ, COLOMBIA LUZ ANDREA MENESES ORTEGÓN TRABAJO DE INVESTIGACIÓN PARA OPTAR AL TÍTULO DE MAGISTER EN CIENCIAS BIOLÓGICAS DIRIGIDO POR: YIMY HERRERA MARTÍNEZ M.Sc. Cand. Ph.D UNIVERSIDAD PEDAGÓGICA Y TECNOLÓGICA DE COLOMBIA FACULTAD DE CIENCIAS BÁSICAS MAESTRÍA EN CIENCIAS BIOLÓGICAS TUNJA 2013 2 Meneses-Ortegón. Bacterioplancton en lagunas altoandinas de Boyacá, Colombia NOTA DE ACEPTACIÓN ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ _____________________________ MARIBEB CASTRO GONZÁLEZ Ph.D GABRIEL A. PINILLA A. Ph.D Jurado Jurado __________________________________ ______________________________ JEOVANNY ARLES GÓMEZ C. Ph.D JAIRO ANTONIO CAMACHO M.Sc Director Escuela de Posgrados Coordinador Académico _________________________________ ______________________________ YIMY HERRERA MARTÍNEZ M.Sc. Ph.D(c) ANDREA MENESES ORTEGÓN Director Autor 3 Meneses-Ortegón. Bacterioplancton en lagunas altoandinas de Boyacá, Colombia DEDICATORIA A mis padres… Con apoyo y paciencia me han acompañado y guiado en el camino de la vida. Los Amo. A mis hermanos… 4 Meneses-Ortegón. Bacterioplancton en lagunas altoandinas de Boyacá, Colombia AGRADECIMIENTOS Hoy, que culmino una etapa más en mi vida, quiero agradecer a todos aquellos que aportaron su granito de arena para que esto se hiciera realidad: A Dios por estar siempre presente y acompañarme en cada paso que doy en la vida.
    [Show full text]
  • How to Cite Complete Issue More Information About This Article
    Revista de Biología Tropical ISSN: 0034-7744 ISSN: 2215-2075 Universidad de Costa Rica Criales-Hernández, María-I.; Sanchez-Lobo, Diana-M.; Almeyda-Osorio, Johanna-K. Expanding the knowledge of plankton diversity of tropical lakes from the Northeast Colombian Andes Revista de Biología Tropical, vol. 68, no. 2, Supl., 2020, September, pp. 159-176 Universidad de Costa Rica DOI: https://doi.org/10.15517/RBT.V68IS2.44347 Available in: https://www.redalyc.org/articulo.oa?id=44967850012 How to cite Complete issue Scientific Information System Redalyc More information about this article Network of Scientific Journals from Latin America and the Caribbean, Spain and Journal's webpage in redalyc.org Portugal Project academic non-profit, developed under the open access initiative ISSN Printed: 0034-7744 ISSN digital: 2215-2075 Expanding the knowledge of plankton diversity of tropical lakes from the Northeast Colombian Andes María I. Criales-Hernández1, Diana M. Sanchez-Lobo1 & Johanna K. Almeyda Osorio1 1. Laboratorio de Hidrobiología, Escuela de biología, Facultad de Ciencias Básicas, Universidad Industrial de Santander, Bucaramanga, Santander, Colombia; [email protected] Received 15-X-2019. Corrected 07-VIII-2020. Accepted 31-VIII-2020. ABSTRACT. Introduction: A large number of planktonic communities found in tropical lakes have not yet been recorded, limiting understanding of how these ecosystems function and of the role that organisms play within them. Objective: Add new records of previously described species and to contribute to the knowledge of the planktonic communities present in tropical mountain and lowland lakes of the northeast Colombian Andes. Methods: Planktonic samples were collected and physicochemical variables measured in nine tropical lakes.
    [Show full text]