Universidad Austral De Chile Facultad De Ciencias Agrarias Escuela De Agronomía

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Universidad Austral De Chile Facultad De Ciencias Agrarias Escuela De Agronomía Universidad Austral de Chile Facultad de Ciencias Agrarias Escuela de Agronomía Efecto de Neotyphodium lolli cepas AR37 y AR1, sobre el desarrollo y sobrevivencia de larvas de cuncunilla negra de las praderas (Dalaca pallens Bl). Memoria presentada como parte de los requisitos para optar al título de Ingeniero Agrónomo José Nicolás Larraín Widmer Valdivia – Chile 2009 PROFESOR PATROCINANTE: ____________________________________ Oscar Balocchi L. Ingeniero Agrónomo, M.Sc.,Ph.D. Instituto de Producción Animal PROFESORES INFORMANTES: ____________________________________ Roberto Carrillo Ll. Ingeniero Agrónomo, M.Sc.,Ph.D. Instituto de Producción y Sanidad Vegetal ___________________________________ Ignacio López C. Ingeniero Agrónomo, Ph.D. Instituto de Producción Animal AGRADECIMIENTOS Al finalizar esta etapa de estudio son muchas las palabras que se vienen a la cabeza al momento de dar las gracias, por el tiempo y la colaboración de un gran número de personas que han ayudado y aportado su granito de arena al terminar este trabajo. Pero principalmente quiero agradecer a las siguientes personas: Al profesor Dr. Roberto Carrillo, por su gran paciencia aportada al momento de preguntas incansables, al profesor Dr. Fernando Mujica, por su gran aporte y comprensión de lo que se quería preguntar, en especial al Laboratorio de Entomología, al Laboratorio de Fitoquímica, al personal del Laboratorio del pabellón de Producción Animal, y como olvidar el gran aporte realizado por un gran profesor y amigo don Oscar Balocchi, el cual con su incansable paciencia respondió a todas las preguntas e inquietudes realizadas por mi parte. Muchísimas gracias i INDICE DE MATERIAS Capítulo Página RESUMEN 1 SUMMARY 2 1 INTRODUCCIÓN 3 2 REVISIÓN BIBLIOGRÁFICA 5 2.1 Importancia de las praderas en el sur de Chile 5 2.2 Las plagas insectiles como problema en la producción de praderas 5 2.3 Control de plagas insectiles 5 2.3.1 Uso de hongos endófitos en ballicas 5 2.3.2 Origen de los hongos endófitos 6 2.3.3 Simbiosis planta-endófito 7 2.3.4 Ciclo de vida del hongo endófito 7 2.3.5 Sobrevivencia del hongo en la semilla 8 2.3.6 Ubicación del endófito en la planta y concentración de alcaloides 8 2.3.7 Compuestos tóxicos producidos por el hongo endófito 9 2.3.7.1 Endófito AR1 10 2.3.7.2 Endófito AR37 10 2.3.8 Determinación del hongo endófito 12 ii 2.3.9 Ballica perenne cultivar Extreme 13 2.4 Las larvas de hepialidos como plagas agrícolas 13 2.4.1 Ciclo biológico y control de Wiseana cervinata usando endófitos en 13 ballica en Nueva Zelanda 2.4.2 El complejo de larvas de cuncunillas negras que infestan praderas 14 en el sur de Chile 2.4.3 Dalaca pallens 15 3 MATERIAL Y METODO 17 3.1 Material 17 3.1.1 Ubicación del ensayo 17 3.1.2 Duración del estudio 17 3.1.3 Material Vegetal 17 3.1.4 Material Animal 17 3.1.5 Materiales de laboratorio e invernadero 17 3.1.6 Instrumentos 17 3.2 Métodos 18 3.2.1 Descripción de los ensayos 18 3.2.1.1 Recolección de las larvas de cuncunilla negra 18 3.2.1.2 Determinación del estado larval de las cuncunillas 18 3.2.1.3 Ensayo 1: Alimentación de las larvas de cuncunilla negra con follaje 18 de plantas de ballica perenne cortadas mecánicamente iii 3.2.1.4 Ensayo 2: Alimentación directa de cuncunillas negras sobre plantas 19 de ballica perenne 3.2.1.5 Variables evaluadas 19 3.2.1.6 Diseño experimental y análisis 20 4 PRESENTACIÓN Y DISCUSIÓN DE RESULTADOS 22 4.1 Ensayo 1 22 4.1.1 Mortalidad larval 22 4.1.2 Incremento en peso de las larvas 23 4.1.3 Medidas capsula cefálicas y estados de desarrollo de larvas de 24 cuncunillas negras 4.2 Ensayo 2 26 4.2.1 Mortalidad de larvas 27 4.2.2 Peso de las cuncunillas 27 4.2.3 Tamaño y estados de las cuncunillas 29 5 CONCLUSIONES 34 6 BIBLIOGRAFÍA 35 iv INDICE DE CUADROS Cuadro Página 1 Niveles y tipos de alcaloides presentes en cultivares de ballica 10 inglesa 2 Peso (g) de larvas de cuncunillas negras en el ensayo de laboratorio 23 3 Medidas cápsula cefálica de larvas de cuncunillas negras 25 (milímetros) 4 Estadios larvarios de las cuncunillas negras 26 5 Peso inicial, final (g/cuncunillas) y diferencia de peso de las 28 cuncunillas negras el 13 de agosto Medida de la capsula cefálica (mm/cuncunilla), inicial y final de las 6 29 cuncunillas negras en la evaluación del 13 de agosto Estadios larvarios inicial y final de las cuncunillas negras en 7 30 evaluación del 13 de agosto 8 Peso inicial, final (g/cuncunilla) y diferencia de peso de las 31 cuncunillas negras en la evaluación del 9 de septiembre Medida de la cápsula cefálica (mm/cuncunilla), inicial y final de las 9 32 cuncunillas negras en la evaluación del 9 de septiembre Estadios larvarios inicial y final de las cuncunillas negras en la 32 10 evaluación del 9 de septiembre v INDICE DE FIGURAS Figura Página 1 Ciclo del hongo endófito en la planta de ballica perenne 8 Efecto del endófito AR 37 en la producción de macollos en ballica 2 11 inglesa 3 Plagas controladas por el endófito AR 37 en Nueva Zelanda 12 4 Ciclo biológico de Wiseana cervinata 14 1 RESUMEN El presente trabajo fue realizado en el Laboratorio de Entomología e invernaderos de la Facultad de Ciencias Agrarias ubicados en el campus Isla Teja de la Universidad Austral de Chile, en la cuidad de Valdivia, durante el periodo marzo a octubre del año 2008. El objetivo del ensayo fue determinar la mortalidad, velocidad y desarrollo en el crecimiento de larvas de cuncunillas negras (Dalaca pallens), alimentadas con plantas de Lolium perenne L. cultivar Extreme, infestadas con las cepas AR1 y AR37 del hongo endófito Neotyphodium lolii en relación a un testigo sin endófito Se realizaron dos ensayos paralelos, el primero se llevó a cabo bajo condiciones controladas de temperatura al interior de un laboratorio, utilizando un diseño completamente al azar con una covariable. El segundo ensayo, que se llevó a cabo en un invernadero, consistió en realizar una evaluación en maceteros con tierra como sustrato, y plantas de Lolium perenne, utilizando un diseño de bloques completos al azar generalizado con una covariable. Los resultados obtenidos demostraron que la presencia de la cepa AR1 y AR37 del hongo endófito (Neotyphodium lolii) en ballica perenne cultivar Extreme no produjeron un aumento en la tasa de mortalidad, ni cambios significativos en el crecimiento y desarrollo de larvas de cuncunilla negra de las praderas (Dalaca pallens). 2 SUMMARY This work was performed at the Laboratory of Entomology and greenhouses in the Faculty of Agricultural Sciences campus located in Isla Teja, Universidad Austral de Chile, in the city of Valdivia, for the period March to October of 2008. The objective of the study was to determine the mortality rate in the growth and development of larvae of black cutworms (Dalaca pallens), fed on plants of Lolium perenne L. Extreme cultivate infested with strains of AR1 and AR37 endophyte Neotyphodium lolii in relation to a control without endophyte. Two parallel trials were conducted, the first conducted under controlled conditions of temperature inside a laboratory, using a completely randomized design with a covariate. The second test is conducted in a greenhouse, consisted of an evaluation in pots with soil as substrate, and plants of perennial ryegrass, using a design of randomized blocks, with a covariable. The results showed that the presence of AR1 and AR37 strain of endophyte (Neotyphodium lolii) in perennial ryegrass cultivar Extreme did not produce an increased mortality rate, no significant change in the growth and development of larvae of black cutworms grasslands (Dalaca pallens). 3 1 INTRODUCCION Las ballicas son un recurso forrajero muy importante para las explotaciones ganaderas tanto a nivel nacional como internacional. Son la base de la alimentación para los diversos tipos de herbívoros ya sean silvestres o domesticados (de uso intensivo) para generar productos usados en el consumo humano. Por lo tanto, es fundamental conocer cuales son los factores que podrían llegar a limitar la producción de dicho recurso, es por esto que se ha investigado a lo largo de los años estos posibles factores, buscando soluciones a estos problemas. Entre los diversos factores estudiados, se ha llegado a la conclusión que uno de los principales problemas es el control de ciertas plagas que afectan negativamente a este tipo de cultivos, donde su ataque disminuye la producción de forraje para los animales. Entre las principales plagas que afectan la producción de forraje de las praderas mejoradas en el sur de Chile, están las cuncunillas negras (complejo de especies del género Dalaca). Probablemente la especie más importante en extensas áreas en el sur de Chile corresponda a Dalaca pallens Bl, insecto que entre los meses de mayo a septiembre causa fuertes pérdidas en la producción de forraje debido a la destrucción total de las plantas o parte de ellas, causando daños cuantitativos y cualitativos que van muchas veces mas allá del período de mayor daño directo. Por lo cual, medidas que reduzcan sus poblaciones y consecuentemente el daño, son relevantes. En otros países como por ejemplo Nueva Zelanda, en los cuales las larvas de hepialidos también son importantes, por reducir la producción de las praderas, entre otras medidas de combate hacia estos insectos se ha optado por la simbiosis entre algunas especies de hongos y plantas, puesto que los hongos producen diferentes grupos de alcaloides que sin interferir en el ciclo normal de la especie vegetal, afecta diferentes plagas.
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