SANTA CRUZ BIOTECHNOLOGY, INC. Type XVIII (H-140): sc-25720

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BACKGROUND APPLICATIONS Type XV and XVIII form the new subgroup , within the Collagen Type XVIII (H-140) is recommended for detection of Collagen α1 diverse family of collagens, which contains nineteen distinct types of collagens Type XVIII of mouse, rat and human origin by Western Blotting (starting found in vertebrates. Both type XV and XVIII collagens are characterized by dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1–2 µg extensive interruptions in their collagenous sequences. Members of the MUL- per 100–500 µg of total (1 ml of cell lysate)] and immunofluores- TIPLEXIN subgroup contain polypeptides with multiple triple-helical domains cence (starting dilution 1:50, dilution range 1:50-1:500). separated and flanked by non-triple-helical regions. Type XV is predominantly Suitable for use as control antibody for Collagen Type XVIII siRNA (h): expressed in internal organs such as adrenal gland, kidney and pancreas. Type sc-43072 and Collagen Type XVIII siRNA (m): sc-43073. XVIII encodes two different α1 chains, which have different signal peptides and variant N-terminal non-collagenous NC1 domains of 495 and 303 amino Molecular Weight of Collagen Type XVIII: 20-22 kDa. acids. The long variant NC1-434 Type XVIII mRNAs are prominently expressed Positive Controls: rat C6 glioblastoma, human PBL or rat lung extract: sc-2396. in liver, while the variant NC1-303 mRNAs are predominantly expressed in heart, kidney, placenta, prostate, ovaries, skeletal muscle and small intestine. RECOMMENDED SECONDARY REAGENTS is a fragment of the C-terminal domain NC1 of collagen XV and XVIII that inhibits angiogenesis and tumor growth. Unlike endostatin-XVIII, To ensure optimal results, the following support (secondary) reagents are endostatin-XV does not bind zinc or heparin, however both can recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004 inhibit chorioallantoic membrane angiogenesis induced by basic FGF or VEGF. (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat anti- Type XV and XVIII collagens are also widely present in rabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™ zones, suggesting their roles in basement membrane-stromal interactions Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: and involvement with angiogenic and pathological processes. sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopreci- pitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). REFERENCES 3) Immunofluorescence: use goat anti-rabbit IgG-FITC: sc-2012 (dilution range: 1:100-1:400) or goat anti-rabbit IgG-TR: sc-2780 (dilution range: 1. Kivirikko, S., et al. 1994. Primary structure of α1 chain of human type XV 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. collagen and exon-intron organization in the 3' region of the corresponding gene. J. Biol. Chem. 269: 4773-4779. DATA 2. Oh, S.P., et al. 1994. Cloning of cDNA and genomic DNA encoding human type XVIII collagen and localization of the 1 (XVIII) collagen gene to mouse ABCDE α 29 K - chromosome 10 and human . Genomics 19: 494-499.

< endostatin 3. Muragaki, Y., et al. 1994. The human α1(XV) collagen chain contains a large 19 K - fragment of amino-terminal non-triple helical domain with a tandem repeat structure Collagen and homology to 1 (XVIII) collagen. J. Biol. Chem. 269: 4042-4046. Type XVIII α 7 K - 4. Myers, J.C., et al. 1997. Biochemical and immunohistochemical character- ization of human type XIX defines a novel class of basement membrane zone collagens. Am. J. Pathol. 151: 1729-1740. Collagen Type XVIII (H-140): sc-25720. Western blot Collagen Type XVIII (H-140): sc-25720. Immunofluo- analysis of endostatin fragment of Collagen Type XVIII rescence staining of methanol-fixed A549 cells expression in rat kidney (A), rat lung (B), rat liver (C), showing cytoplasmic localization. 5. Saarela, J., et al. 1998. Complete primary structure of two variant forms of mouse pancreas (D) and rat pancreas (E) tissue extracts. human type XVIII collagen and tissue-specific differences in the expression of the corresponding transcripts. Matrix Biol. 16: 319-328. STORAGE CHROMOSOMAL LOCATION Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Genetic locus: COL18A1 (human) mapping to 21q22.3; Col18a1 (mouse) mapping to 10. RESEARCH USE SOURCE For research use only, not for use in diagnostic procedures. Collagen Type XVIII (H-140) is a rabbit polyclonal antibody raised against PROTOCOLS amino acids 1201-1340 of Collagen α1 Type XVIII of human origin. See our web site at www.scbt.com or our catalog for detailed protocols PRODUCT and support products. Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com