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S1 Sulfate Reducing Bacteria and Mycobacteria Dominate the Biofilm
Sulfate Reducing Bacteria and Mycobacteria Dominate the Biofilm Communities in a Chloraminated Drinking Water Distribution System C. Kimloi Gomez-Smith 1,2 , Timothy M. LaPara 1, 3, Raymond M. Hozalski 1,3* 1Department of Civil, Environmental, and Geo- Engineering, University of Minnesota, Minneapolis, Minnesota 55455 United States 2Water Resources Sciences Graduate Program, University of Minnesota, St. Paul, Minnesota 55108, United States 3BioTechnology Institute, University of Minnesota, St. Paul, Minnesota 55108, United States Pages: 9 Figures: 2 Tables: 3 Inquiries to: Raymond M. Hozalski, Department of Civil, Environmental, and Geo- Engineering, 500 Pillsbury Drive SE, Minneapolis, MN 554555, Tel: (612) 626-9650. Fax: (612) 626-7750. E-mail: [email protected] S1 Table S1. Reference sequences used in the newly created alignment and taxonomy databases for hsp65 Illumina sequencing. Sequences were obtained from the National Center for Biotechnology Information Genbank database. Accession Accession Organism name Organism name Number Number Arthrobacter ureafaciens DQ007457 Mycobacterium koreense JF271827 Corynebacterium afermentans EF107157 Mycobacterium kubicae AY373458 Mycobacterium abscessus JX154122 Mycobacterium kumamotonense JX154126 Mycobacterium aemonae AM902964 Mycobacterium kyorinense JN974461 Mycobacterium africanum AF547803 Mycobacterium lacticola HM030495 Mycobacterium agri AY438080 Mycobacterium lacticola HM030495 Mycobacterium aichiense AJ310218 Mycobacterium lacus AY438090 Mycobacterium aichiense AF547804 Mycobacterium -
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International Journal of Current Advanced Research ISSN: O: 2319-6475, ISSN: P: 2319 – 6505, Impact Factor: SJIF: 5.995 Available Online at www.journalijcar.org Volume 6; Issue 9; September 2017; Page No. 5982-5985 DOI: http://dx.doi.org/10.24327/ijcar.2017.5985.0846 Reserach Article ANTIMYCOTIC ACTIVITY OF FLOWER EXTRACT OF CRATAEVA NURVALA BUCH-HAM Rajesh Kumar* Centre of Rural Technology & Development, Department of Botany, Faculty of Science, University of Allahabad, Allahabad-211002 ARTICLE INFO ABSTRACT Article History: Dermal mycotic infections caused by superficial fungi are most prevalent disease of body surface. Dermatophytes comprising of three genera are responsible for these types of Received 4th June, 2017 infections in human beings and other animals. The aim of present study was to evaluate Received in revised form 3rd the antimycotic activity of 50 % ethanolic extract of Crataeva nurvala (extracted by July, 2017 Accepted 24th August, 2017 rotavapor process) using the technique of Broth Micro Dilution method, recommended by Published online 28th September, 2017 CLSI (NCCLS). The activities were analysed in units of MIC having 1.511 and 1.981 mg/ml for Trichophyton mentagrophytes and Microsporum fulvum respectively. The Key words: microbial activity of the Crataeva nurvala was due to the presence of various secondary metabolites. Further studies will to helpful to isolate the active compounds from those Dermatophytes, antimycotic activity, rotavapor, extracts with fungicidal potential. MIC Copyright©2017 Rajesh Kumar. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. -
Phylogenetic Analysis of Dermatophyte Species Using DNA Sequence Polymorphism in Calmodulin Gene Bahram Ahmadi1,2, Hossein Mirhendi3,∗, Koichi Makimura4, G
Medical Mycology Advance Access published February 11, 2016 Medical Mycology, 2016, 0, 1–15 doi: 10.1093/mmy/myw004 Advance Access Publication Date: 0 2016 Original Article Original Article Phylogenetic analysis of dermatophyte species using DNA sequence polymorphism in calmodulin gene Bahram Ahmadi1,2, Hossein Mirhendi3,∗, Koichi Makimura4, G. Sybren de Hoog5, Mohammad Reza Shidfar2, 6 2 Sadegh Nouripour-Sisakht and Niloofar Jalalizand Downloaded from 1Department of Microbiology and Parasitology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran, 2Departments of Medical Parasitology & Mycology, School of Public Health; National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran, 3Departments of Medical Parasitology & Mycology, School of Medicine, Isfahan University of Medical Sciences, http://mmy.oxfordjournals.org/ Isfahan, Iran, 4Teikyo University Institute of Medical Mycology and Genome Research Center, Tokyo, Japan, 5Fungal Biodiversity Center, Institute of the Royal Netherlands, Academy of Arts and Sciences, Centraalbureau voor Schimmelcultures-KNAW, Utrecht, The Netherlands and 6Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran ∗To whom correspondence should be addressed. Hossein Mirhendi, Professor, Department of Medical Parasitology and Mycology, School of Medicine; Isfahan University of Medical Sciences, Isfahan, Iran. Tel/Fax: +00982188951392; E-mail: [email protected]. by guest on February 12, 2016 Received 23 October 2015; Revised 23 December 2015; Accepted 5 January 2016 Abstract Use of phylogenetic species concepts based on rDNA internal transcribe spacer (ITS) regions have improved the taxonomy of dermatophyte species; however, confirmation and refinement using other genes are needed. Since the calmodulin gene has not been systematically used in dermatophyte taxonomy, we evaluated its intra- and interspecies sequence variation as well as its application in identification, phylogenetic analysis, and taxonomy of 202 strains of 29 dermatophyte species. -
Mycobacterium Avium Subespecie Paratuberculosis. Mapa Epidemiológico En España
UNIVERSIDAD COMPLUTENSE DE MADRID FACULTAD DE VETERINARIO DEPARTAMENTO DE SANIDAD ANIMAL TESIS DOCTORAL Caracterización molecular de aislados de Mycobacterium avium subespecie paratuberculosis. Mapa epidemiológico en España MEMORIA PARA OPTAR AL GRADO DE DOCTOR PRESENTADA POR Elena Castellanos Rizaldos Directores: Alicia Aranaz Martín Lucas Domínguez Rodríguez Lucía de Juan Ferré Madrid, 2010 ISBN: 978-84-693-7626-3 © Elena Castellanos Rizaldos, 2010 FACULTAD DE VETERINARIA DEPARTAMENTO DE SANIDAD ANIMAL Y CENTRO DE VIGILANCIA SANITARIA VETERINARIA (VISAVET) Caracterización molecular de aislados de Mycobacterium avium subespecie paratuberculosis. Mapa epidemiológico en España Elena Castellanos Rizaldos MEMORIA PARA OPTAR AL GRADO DE DOCTOR EUROPEO POR LA UNIVERSIDAD COMPLUTENSE DE MADRID Facultad de Veterinaria Departamento de Sanidad Animal y Centro de Vigilancia Sanitaria Veterinaria (VISAVET) Dña. Alicia Aranaz Martín, Profesora contratada doctor, D. Lucas Domínguez Rodríguez, Catedrático y Dña. Lucía de Juan Ferré, Profesor Ayudante del Departamento de Sanidad Animal de la Facultad de Veterinaria. CERTIFICAN: Que la tesis doctoral “Caracterización molecular de Mycobacterium avium subespecie paratuberculosis. Mapa epidemiológico en España” ha sido realizada por la licenciada en Veterinaria Dña. Elena Castellanos Rizaldos en el Departamento de Sanidad Animal de la Facultad de Veterinaria de la Universidad Complutense de Madrid y en el Centro de Vigilancia Sanitaria Veterinaria (VISAVET) bajo nuestra dirección y estimamos que reúne los requisitos exigidos para optar al Título de Doctor por la Universidad Complutense de Madrid. Parte de esta tesis ha sido realizada en la Saint George’s University de Londres, Reino Unido y la University of Calgary, Canadá. La financiación del trabajo se realizó mediante los proyectos AGL2005-07792 del Ministerio de Ciencia e Innovación, el proyecto europeo ParaTBTools FP6-2004-FOOD-3B-023106 y la beca de Formación de Profesorado Universitario (F. -
Redalyc.Historia Y Descripción De Microsporum Fulvum, Una Especie
Revista Argentina de Microbiología ISSN: 0325-7541 [email protected] Asociación Argentina de Microbiología Argentina NEGRONI, R.; BONVEHI, P.; ARECHAVALA, A. Historia y descripción de Microsporum fulvum, una especie válida del género descubierta en la República Argentina Revista Argentina de Microbiología, vol. 40, núm. 1, 2008, p. 47 Asociación Argentina de Microbiología Buenos Aires, Argentina Disponible en: http://www.redalyc.org/articulo.oa?id=213016786010 Cómo citar el artículo Número completo Sistema de Información Científica Más información del artículo Red de Revistas Científicas de América Latina, el Caribe, España y Portugal Página de la revista en redalyc.org Proyecto académico sin fines de lucro, desarrollado bajo la iniciativa de acceso abierto Imágenes microbiológicas ISSN 0325-754147 IMÁGENES MICROBIOLÓGICAS Revista Argentina de Microbiología (2008) 40: 47 Historia y descripción de Microsporum fulvum, una especie válida del género descubierta en la República Argentina Se presentan estas imágenes para destacar el interés de una especie válida del género Microsporum descrita por primera vez en 1909 por el dermatólogo argentino Julio Uriburu. Este espe- cialista formó parte del grupo inicial de médicos dedicados a la dermatología que fundaron la Asociación Argentina de Derma- tología, en 1907. Presentamos aquí al grupo de fundadores, entre los cuales se destacan, además de Uriburu, Pedro Baliña, Baldomero Sommer, Maximiliano Aberastury, Nicolás V. Greco y Pacífico Díaz. Este aislamiento corresponde al cultivo de una uña de pie, que es una localización sumamente infrecuente para hongos del género Microsporum. Debido a la similitud morfológica de esta especie con Figura 1. Fundadores de la Asociación Argentina de Dermatología Microsporum gypseum, algunos autores no aceptan su validez y De pie: Julio V. -
Prevalence, Etiology, Public Health Importance and Economic Impact of Mycobacteriosis in Slaughter Cattle in Laikipia County, Kenya
PREVALENCE, ETIOLOGY, PUBLIC HEALTH IMPORTANCE AND ECONOMIC IMPACT OF MYCOBACTERIOSIS IN SLAUGHTER CATTLE IN LAIKIPIA COUNTY, KENYA. A thesis submitted to the University of Nairobi in partial fulfilment of the Masters of Science in Veterinary Epidemiology and Economics degree of University of Nairobi By AKWALU SAMUEL KAMWILU (BVM) Department of Public Health, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi 2019 i DECLARATION This thesis is my original work and has not been presented for a degree in any other University. Signature………………………………… Date…………………………………… AKWALU SAMUEL KAMWILU (BVM) This thesis has been submitted for examination with our approval as supervisors. Signature………………………………… Date…………………………………… DR. KURIA, J.K.N.(BVM,MSc,PhD) Department of Veterinary Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi. Signature………………………………… Date…………………………………… PROF. OMBUI, J.N.(BVM,MSc,PhD) Department of Veterinary Public Health, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi. ii DEDICATION This work is dedicated to: My dear wife Dorothy, our children Kimathi, Munene and Karimi and My parents The late Mr. M’Akwalu and my loving mother, Sarah Akwalu. iii ACKNOWLEDGEMENT This work was carried out at the National Tuberculosis Reference Laboratory (NTRL), Central Veterinary Laboratories, Kabete (CVL,Kabete) and the Department of Veterinary Public Health, Pharmacology, and Toxicology, University of Nairobi. I’m very grateful to Dr. Kuria, J.K.N. my major supervisor for his guidance in conceptualizing this work, advice, accompanying me to the NTRL and CVL laboratories, helping in manual work many times and revision and correction of the manuscript. I’m also grateful to Prof. -
Récente Révision Des Espèces De Dermatophytes Et De Leur Nomenclature
DERMATOLOGIE Récente révision des espèces de dermatophytes et de leur nomenclature Dr MICHEL MONOD a Rev Med Suisse 2017 ; 13 : 703-8 L’identification des dermatophytes est souvent compliquée par morphologiques qui existent au sein d’une même espèce. De la variabilité de leurs caractères en culture et des problèmes de surcroît, des isolats d’espèces différentes peuvent présenter nomenclature. L’analyse d’un ensemble de séquences d’ADN a le même aspect. A cela s’ajoutent des problèmes de nomen permis de redéfinir les genres et les espèces de ces champignons clature avec plusieurs noms pour la même espèce (synonymes), spécialisés. Les noms d’espèces ont été révisés en accord avec la et avec une pléthore d’espèces décrites.3 nouvelle convention adoptée pour la nomenclature des champi- gnons, et avec le soin de ne pas chambouler tous les usages. Les La taxonomie des dermatophytes au niveau des genres et des conclusions de cette étude et les noms d’espèces à utiliser ont espèces a été récemment revisitée sur la base d’analyse de sé été approuvés par un ensemble d’experts praticiens ou fonda- quences d’ADN.3 La nomenclature a été révisée en accord mentalistes travaillant avec ce groupe de champignons. Les avec la nouvelle convention adoptée pour celle des espèces de points importants concernant la définition d’espèces et quelques champignons4 et le soin de préserver au maximum les noms changements de nomenclature ont été résumés dans cet article. utilisés en pratique. L’objectif de cet article est de présenter les aboutissements de cette révision et de donner les noms d’espèces qui devraient être dorénavant adoptés par les labo Revision of the dermatophyte species and the ratoires, par les médecins et dans la littérature. -
Antifungal Susceptibility of Japanese Isolates of Nannizia Fulva (Formerly Microsporum Fulvum)
Med. Mycol. J. Vol.Med. 60, Mycol.23-25, 2019 J. Vol. 60 (No. 1) , 2019 23 ISSN 2185-6486 Short Report Antifungal Susceptibility of Japanese Isolates of Nannizia fulva (Formerly Microsporum fulvum) Rui Kano1, Karin Oshimo1, Teru Fukutomi2 and Hiroshi Kamata1 1 Department of Veterinary Pathobiology, Nihon University College of Bioresouce Sciences School of Veterinary Medicine 2 Bright Pet Clinic ABSTRACT Human and animal dermatophytoses are most commonly treated with systemic antifungal drugs such as itraconazole (ITZ) and terbinafine (TRF). The antifungal susceptibility of Nannizia fulva, however, remains poorly documented. In the present study, we investigated the in vitro susceptibility of N. fulva to ITZ and TRFusing the CLSI M38-A2 test. The mean MICs for the 12 tested strains were 0.6542 mg/L (range: 0.0625-1 mg/L) for ITZ and 0.15625 mg/L (range: < 0.003125-0.5 mg/L) for TRF. These results indicate that ITZ and TRFat standard veterinary doses should be efficacious against N. fulva. Key words : Antifungal susceptibility, geophilic dermatophyte, itraconazole, Nannizia fulva, terbinafine Members of the Microsporum gypseum complex are (Table 1). geophilic dermatophytes with worldwide distribution and The isolates of N. fulva examined in this study are listed in occasionally have been isolated as infectious agents in humans Table 1. These isolates were obtained from normal rabbit hair and animals1-3). The teleomorphs of the complex consist of and soils in rabbit hutches in public primary schools in Nannizia fulva (formerly Microsporum fulvum and Yokohama, Japan6). Arthroderma fulvum), N. gypsea,andNannizia. incurvata1, 4). The isolates were maintained on diluted Sabouraud’s In 1982, Hironaga et al. -
View Covering the Agent and the Disease It Causes in Fish and Humans
Gcebe et al. BMC Microbiology (2018) 18:32 https://doi.org/10.1186/s12866-018-1177-9 RESEARCH ARTICLE Open Access Non-tuberculous Mycobacterium species causing mycobacteriosis in farmed aquatic animals of South Africa Nomakorinte Gcebe1* , Anita L. Michel2 and Tiny Motlatso Hlokwe1 Abstract Background: Mycobacteriosis caused by non-tuberculous mycobacteria (NTM), is among the most chronic diseases of aquatic animals. In addition, fish mycobacteriosis has substantial economic consequences especially in the aquaculture and fisheries industry as infections may significantly decrease production and trade. Some fish NTM pathogens are highly virulent and zoonotic; as such, infection of aquaria with these pathogens is a public health concern. In this study, we report isolation of nine different NTM species from sixteen aquatic animals including different fish species, frogs and a crocodile. Given the clinical significance of Mycobacterium marinum and its close relation to Mycobacterium tuberculosis, as well as the significance of ESAT 6 and CFP-10 secretion in mycobacterial virulence, we analysed the esxA and esxB nucleotide sequences of M. marinum isolates identified in this study as well as other mycobacteria in the public databases. Results: Mycobacterium shimoidei, Mycobacterium marinum, Mycobacterium chelonae, Mycobacterium septicum /M. peregrinum and Mycobacterium porcinum were isolated from gold fish, Guppy, exotic fish species in South Africa, koi and undefined fish, Knysna seahorse, as well Natal ghost frogs respectively, presenting tuberculosis like granuloma. Other NTM species were isolated from the studied aquatic animals without any visible lesions, and these include Mycobacterium sp. N845 T, Mycobacterium fortuitum, a member of the Mycobacterium avium complex, and Mycobacterium szulgai. Phylogenetic analysis of mycobacteria, based on esxA and esxB genes, separated slow growing from rapidly growing mycobacteria as well as pathogenic from non-pathogenic mycobacteria in some cases. -
PRP8 Intein in Dermatophytes: Evolution and Species Identification
Medical Mycology, 2018, 56, 746–758 doi: 10.1093/mmy/myx102 Advance Access Publication Date: 8 December 2017 Original Article Downloaded from https://academic.oup.com/mmy/article-abstract/56/6/746/4714803 by Universidade Estadual Paulista J�lio de Mesquita Filho user on 16 August 2019 Original Article PRP8 intein in dermatophytes: Evolution and species identification Hans Garcia Garces1, Raquel Theodoro Cordeiro2 and E. Bagagli1,∗ 1Departamento de Microbiologia e Imunologia, Instituto de Biociencias,ˆ Universidade Estadual de Sao˜ Paulo. Sao˜ Paulo. Brasil and 2Instituto de Medicina Tropical do RN, Universidade Federal de Rio Grande do Norte. Rio Grande do Norte. Brasil ∗To whom correspondence should be addressed. Eduardo Bagagli, PhD, Department of Microbiology and Immunology Institute of Biosciences, UNESP Sao˜ Paulo State University, Campus at Botucatu, Sao Paulo, Brazil. E-mail: [email protected] Received 10 May 2017; Revised 4 July 2017; Accepted 27 September 2017; Editorial Decision 9 August 2017 Abstract Dermatophytes are keratinophilic fungi belonging to the family Arthrodermataceae.De- spite having a monophyletic origin, its systematics has always been complex and con- troversial. Sequencing of nuclear ribosomal ITS and D1/D2 rDNA has been proposed as an efficient tool for identifying species in this group of fungi, while multilocus analy- ses have been used for phylogenetic species recognition. However, the search for new markers, with sequence and size variation, which enable species identification in only one polymerase chain reaction (PCR) step, is very attractive. Inteins seems to fulfill these characteristics. They are self-splicing genetic elements present within housekeeping cod- ing genes, such as PRP8, that codify the most important protein of the spliceosome. -
Biocompatible Antidermatophytic Scaffolds (Tfg-Nf) for Controlled and Impressive Management of Topical Tinea Diseases
Acta Scientific Pharmaceutical Sciences (ISSN: 2581-5423) Volume 5 Issue 7 July 2021 Research Article Biocompatible Antidermatophytic Scaffolds (TfG-Nf) for Controlled and Impressive Management of Topical Tinea Diseases Shashi Kiran Misra1*, Himanshu Pandey2, Kamla Pathak3 and Sandip Received: May 24, 2021 Patil4 Published: June 03, 2021 1University Institute of Pharmacy, CSJMU, Kanpur, India © All rights are reserved by Shashi Kiran 2Central University of Higher Tibetan Studies, Varanasi, India Misra., et al. 3Faculty of Pharmacy, Uttar Pradesh University of Medical Sciences, Saifai, Uttar Pradesh, India 4E-Spin NanoTech Private Ltd., SIDBI Innovation and Incubation Center, Indian Institute of Technology, Kanpur, India *Corresponding Author: Shashi Kiran Misra, University Institute of Pharmacy, CSJMU, Kanpur, India. Abstract Purpose: The rationale behind this study was to develop graphene based tolnaftate conjugate (Tf G) and embed it within scaffolds composed of biocompatible Eudragit polymers for controlled and impressive antitinea activity. Methods: Developed Tf G conjugate was entrenched in 20% w/v polymeric solution (ERL100/ERS100) and nonwoven scaffolds through E-spin (electrospinning) technology were fabricated. Assorted analytical techniques i.e. FESEM, FTIR, XRD and DSC were employed to characterize and assessment of scaffolds. Sessile drop and Dialysis bag methods were utilized for the determination of their hydrophilicity and drug release behavior. In vitro fungal study was performed on enormously virulent strains of tinea infections i.e. zoophilic Microsporum fulvum and anthropophilic Trichophyton rubrum. Animal study was performed on Trichophyton rubrum diseased Swiss albino mice for seven days. Results: High payload of Tf on G was perceived by the virtue of extensive surface area of G and process of physisorption. -
INFECTIOUS DISEASE Short Title: Disseminated Mycobacteriosis In
INFECTIOUS DISEASE Short Title: Disseminated Mycobacteriosis in Cats Non-tuberculous Mycobacteria can Cause Disseminated Mycobacteriosis in Cats H. Pekkarinen,⃰ N. Airas⃰ , L. E. Savolainen†, M. Rantala‡, S. Kilpinen‡, O. Miuku‡, M. Speeti§, V. Karkamo¶, S. Malkamäki*, M. Vaara†, A. Sukura⃰ and P. Syrjä* ⃰ Department of Veterinary Biosciences, Faculty of Veterinary Medicine, PO Box 66, University of Helsinki, †Department of Clinical Microbiology, University of Helsinki and Helsinki University Hospital, HUSLAB, Helsinki, Finland, ‡Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, PO Box 57, University of Helsinki, §Herttoniemi Veterinary Clinic, Hiihtomäentie 35, Helsinki and ¶Pathology Research Unit, Finnish Food Safety Authority Evira, Mustialankatu 3, Helsinki, Finland. Correspondence to: H. Pekkarinen (e-mail: [email protected]). Summary Mycobacteriosis caused by non-tuberculous mycobacteria (NTM) is a rising concern in human medicine both in immunocompromised and immunocompetent patients. In cats, mycobacteriosis caused by NTM is considered mostly to be a focal or dermal infection, with disseminated disease mostly caused by Mycobacterium avium. We describe three cases of disseminated mycobacteriosis in cats, caused by M. malmoense, M. branderi/shimoidei and M. avium, with no identified underlying immunosuppression. In all cases, extracellular mycobacteria were seen in the pulmonary epithelium, intestinal lumen and glomerular tufts, which could affect the shedding of the organism. The present study highlights the importance of mycobacteriosis as a differential even in immunocompetent animals. Considering the close relationship of owners and pets and the potential presence of free mycobacteria in secretions, cats should be considered as a possible environmental reservoir for mycobacteria. Keywords: mycobacteriosis; cat; non-tuberculous mycobacteria Introduction Mycobacteria are acid-fast, aerobic, non-spore forming rod shaped bacteria that range from obligate pathogens to environmental saprophytes.