Mycobacterium Xenopi and Related Organisms Isolated from Stream Waters in Finland and Description of Mycobacterium Botniense Sp
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Accuprobe Mycobacterium Avium Complex Culture
non-hybridized and hybridized probe. The labeled DNA:RNA hybrids are measured in a Hologic luminometer. A positive result is a luminometer reading equal to or greater than the cut-off. A value below this cut-off is AccuProbe® a negative result. REAGENTS Note: For information on any hazard and precautionary statements that MYCOBACTERIUM AVIUM may be associated with reagents, refer to the Safety Data Sheet Library at www.hologic.com/sds. COMPLEX CULTURE Reagents for the ACCUPROBE MYCOBACTERIUM AVIUM COMPLEX IDENTIFICATION TEST CULTURE IDENTIFICATION TEST are provided in three separate reagent kits: INTENDED USE The ACCUPROBE MYCOBACTERIUM AVIUM COMPLEX CULTURE ACCUPROBE MYCOBACTERIUM AVIUM COMPLEX PROBE KIT IDENTIFICATION TEST is a rapid DNA probe test which utilizes the Probe Reagent. (4 x 5 tubes) technique of nucleic acid hybridization for the identification of Mycobacterium avium complex Mycobacterium avium complex (M. avium complex) isolated from culture. Lysing Reagent. (1 x 20 tubes) Glass beads and buffer SUMMARY AND EXPLANATION OF THE TEST Infections caused by members of the M. avium complex are the most ACCUPROBE CULTURE IDENTIFICATION REAGENT KIT common mycobacterial infections associated with AIDS and other Reagent 1 (Lysis Reagent). 1 x 10 mL immunocompromised patients (7,15). The incidence of M. avium buffered solution containing 0.04% sodium azide complex as a clinically significant pathogen in cases of chronic pulmonary disease is also increasing (8,17). Recently, several Reagent 2 (Hybridization Buffer). 1 x 10 mL laboratories have reported that the frequency of isolating M. avium buffered solution complex is equivalent to or greater than the frequency of isolating M. -
S1 Sulfate Reducing Bacteria and Mycobacteria Dominate the Biofilm
Sulfate Reducing Bacteria and Mycobacteria Dominate the Biofilm Communities in a Chloraminated Drinking Water Distribution System C. Kimloi Gomez-Smith 1,2 , Timothy M. LaPara 1, 3, Raymond M. Hozalski 1,3* 1Department of Civil, Environmental, and Geo- Engineering, University of Minnesota, Minneapolis, Minnesota 55455 United States 2Water Resources Sciences Graduate Program, University of Minnesota, St. Paul, Minnesota 55108, United States 3BioTechnology Institute, University of Minnesota, St. Paul, Minnesota 55108, United States Pages: 9 Figures: 2 Tables: 3 Inquiries to: Raymond M. Hozalski, Department of Civil, Environmental, and Geo- Engineering, 500 Pillsbury Drive SE, Minneapolis, MN 554555, Tel: (612) 626-9650. Fax: (612) 626-7750. E-mail: [email protected] S1 Table S1. Reference sequences used in the newly created alignment and taxonomy databases for hsp65 Illumina sequencing. Sequences were obtained from the National Center for Biotechnology Information Genbank database. Accession Accession Organism name Organism name Number Number Arthrobacter ureafaciens DQ007457 Mycobacterium koreense JF271827 Corynebacterium afermentans EF107157 Mycobacterium kubicae AY373458 Mycobacterium abscessus JX154122 Mycobacterium kumamotonense JX154126 Mycobacterium aemonae AM902964 Mycobacterium kyorinense JN974461 Mycobacterium africanum AF547803 Mycobacterium lacticola HM030495 Mycobacterium agri AY438080 Mycobacterium lacticola HM030495 Mycobacterium aichiense AJ310218 Mycobacterium lacus AY438090 Mycobacterium aichiense AF547804 Mycobacterium -
Mycobacterium Avium Subespecie Paratuberculosis. Mapa Epidemiológico En España
UNIVERSIDAD COMPLUTENSE DE MADRID FACULTAD DE VETERINARIO DEPARTAMENTO DE SANIDAD ANIMAL TESIS DOCTORAL Caracterización molecular de aislados de Mycobacterium avium subespecie paratuberculosis. Mapa epidemiológico en España MEMORIA PARA OPTAR AL GRADO DE DOCTOR PRESENTADA POR Elena Castellanos Rizaldos Directores: Alicia Aranaz Martín Lucas Domínguez Rodríguez Lucía de Juan Ferré Madrid, 2010 ISBN: 978-84-693-7626-3 © Elena Castellanos Rizaldos, 2010 FACULTAD DE VETERINARIA DEPARTAMENTO DE SANIDAD ANIMAL Y CENTRO DE VIGILANCIA SANITARIA VETERINARIA (VISAVET) Caracterización molecular de aislados de Mycobacterium avium subespecie paratuberculosis. Mapa epidemiológico en España Elena Castellanos Rizaldos MEMORIA PARA OPTAR AL GRADO DE DOCTOR EUROPEO POR LA UNIVERSIDAD COMPLUTENSE DE MADRID Facultad de Veterinaria Departamento de Sanidad Animal y Centro de Vigilancia Sanitaria Veterinaria (VISAVET) Dña. Alicia Aranaz Martín, Profesora contratada doctor, D. Lucas Domínguez Rodríguez, Catedrático y Dña. Lucía de Juan Ferré, Profesor Ayudante del Departamento de Sanidad Animal de la Facultad de Veterinaria. CERTIFICAN: Que la tesis doctoral “Caracterización molecular de Mycobacterium avium subespecie paratuberculosis. Mapa epidemiológico en España” ha sido realizada por la licenciada en Veterinaria Dña. Elena Castellanos Rizaldos en el Departamento de Sanidad Animal de la Facultad de Veterinaria de la Universidad Complutense de Madrid y en el Centro de Vigilancia Sanitaria Veterinaria (VISAVET) bajo nuestra dirección y estimamos que reúne los requisitos exigidos para optar al Título de Doctor por la Universidad Complutense de Madrid. Parte de esta tesis ha sido realizada en la Saint George’s University de Londres, Reino Unido y la University of Calgary, Canadá. La financiación del trabajo se realizó mediante los proyectos AGL2005-07792 del Ministerio de Ciencia e Innovación, el proyecto europeo ParaTBTools FP6-2004-FOOD-3B-023106 y la beca de Formación de Profesorado Universitario (F. -
Piscine Mycobacteriosis
Piscine Importance The genus Mycobacterium contains more than 150 species, including the obligate Mycobacteriosis pathogens that cause tuberculosis in mammals as well as environmental saprophytes that occasionally cause opportunistic infections. At least 20 species are known to Fish Tuberculosis, cause mycobacteriosis in fish. They include Mycobacterium marinum, some of its close relatives (e.g., M. shottsii, M. pseudoshottsii), common environmental Piscine Tuberculosis, organisms such as M. fortuitum, M. chelonae, M. abscessus and M. gordonae, and Swimming Pool Granuloma, less well characterized species such as M. salmoniphilum and M. haemophilum, Fish Tank Granuloma, among others. Piscine mycobacteriosis, which has a range of outcomes from Fish Handler’s Disease, subclinical infection to death, affects a wide variety of freshwater and marine fish. It Fish Handler’s Nodules has often been reported from aquariums, research laboratories and fish farms, but outbreaks also occur in free-living fish. The same organisms sometimes affect other vertebrates including people. Human infections acquired from fish are most often Last Updated: November 2020 characterized by skin lesions of varying severity, which occasionally spread to underlying joints and tendons. Some lesions may be difficult to cure, especially in those who are immunocompromised. Etiology Mycobacteriosis is caused by members of the genus Mycobacterium, which are Gram-positive, acid fast, pleomorphic rods in the family Mycobacteriaceae and order Actinomycetales. This genus is traditionally divided into two groups: the members of the Mycobacterium tuberculosis complex (e.g., M. tuberculosis, M. bovis, M. caprae, M. pinnipedii), which cause tuberculosis in mammals, and the nontuberculous mycobacteria. The organisms in the latter group include environmental saprophytes, which sometimes cause opportunistic infections, and other species such as M. -
Accepted Manuscript
Genome-based taxonomic revision detects a number of synonymous taxa in the genus Mycobacterium Item Type Article Authors Tortoli, E.; Meehan, Conor J.; Grottola, A.; Fregni Serpini, J.; Fabio, A.; Trovato, A.; Pecorari, M.; Cirillo, D.M. Citation Tortoli E, Meehan CJ, Grottola A et al (2019) Genome-based taxonomic revision detects a number of synonymous taxa in the genus Mycobacterium. Infection, Genetics and Evolution. 75: 103983. Rights © 2019 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http:// creativecommons.org/licenses/by-nc-nd/4.0/) Download date 29/09/2021 07:10:28 Link to Item http://hdl.handle.net/10454/17474 Accepted Manuscript Genome-based taxonomic revision detects a number of synonymous taxa in the genus Mycobacterium Enrico Tortoli, Conor J. Meehan, Antonella Grottola, Giulia Fregni Serpini, Anna Fabio, Alberto Trovato, Monica Pecorari, Daniela M. Cirillo PII: S1567-1348(19)30201-1 DOI: https://doi.org/10.1016/j.meegid.2019.103983 Article Number: 103983 Reference: MEEGID 103983 To appear in: Infection, Genetics and Evolution Received date: 13 June 2019 Revised date: 21 July 2019 Accepted date: 25 July 2019 Please cite this article as: E. Tortoli, C.J. Meehan, A. Grottola, et al., Genome-based taxonomic revision detects a number of synonymous taxa in the genus Mycobacterium, Infection, Genetics and Evolution, https://doi.org/10.1016/j.meegid.2019.103983 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. -
Prevalence, Etiology, Public Health Importance and Economic Impact of Mycobacteriosis in Slaughter Cattle in Laikipia County, Kenya
PREVALENCE, ETIOLOGY, PUBLIC HEALTH IMPORTANCE AND ECONOMIC IMPACT OF MYCOBACTERIOSIS IN SLAUGHTER CATTLE IN LAIKIPIA COUNTY, KENYA. A thesis submitted to the University of Nairobi in partial fulfilment of the Masters of Science in Veterinary Epidemiology and Economics degree of University of Nairobi By AKWALU SAMUEL KAMWILU (BVM) Department of Public Health, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi 2019 i DECLARATION This thesis is my original work and has not been presented for a degree in any other University. Signature………………………………… Date…………………………………… AKWALU SAMUEL KAMWILU (BVM) This thesis has been submitted for examination with our approval as supervisors. Signature………………………………… Date…………………………………… DR. KURIA, J.K.N.(BVM,MSc,PhD) Department of Veterinary Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi. Signature………………………………… Date…………………………………… PROF. OMBUI, J.N.(BVM,MSc,PhD) Department of Veterinary Public Health, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi. ii DEDICATION This work is dedicated to: My dear wife Dorothy, our children Kimathi, Munene and Karimi and My parents The late Mr. M’Akwalu and my loving mother, Sarah Akwalu. iii ACKNOWLEDGEMENT This work was carried out at the National Tuberculosis Reference Laboratory (NTRL), Central Veterinary Laboratories, Kabete (CVL,Kabete) and the Department of Veterinary Public Health, Pharmacology, and Toxicology, University of Nairobi. I’m very grateful to Dr. Kuria, J.K.N. my major supervisor for his guidance in conceptualizing this work, advice, accompanying me to the NTRL and CVL laboratories, helping in manual work many times and revision and correction of the manuscript. I’m also grateful to Prof. -
Mycolicibacterium Stellarae
© Nouioui, I., Sangal, V., Cortés-Albayay, C., Jando, M., Igual, J.M., Klenk, H.P.; Zhang, Y.Q., Goodfellow, M. 2019. The definitive peer reviewed, edited version of this article is published in International Journal of Systematic and Evolutionary Microbiology, 69, 11, 2019, http://dx.doi.org/10.1099/ijsem.0.003644 Mycolicibacterium stellerae sp. nov., a rapidly growing scotochromogenic strain isolated from Stellera chamaejasme Imen Nouioui 1* , Vartul Sangal 2, Carlos Cortés-Albayay 1, Marlen Jando 3, José Mariano Igual 4, Hans-Peter Klenk 1, Yu-Qin Zhang 5, Michael Goodfellow 1 1School of Natural and Environmental Sciences, Ridley Building 2, Newcastle University,Newcastle upon Tyne, NE1 7RU, United Kingdom 2Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK 3Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany 4Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), c/Cordel de Merinas 40-52, 37008 Salamanca, Spain 5Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 of Tiantanxili Road, Beijing, Beijing 100050, China Corresponding author : Imen Nouioui [email protected] Section: Actinobacteria Keywords: Actinobacteria, polyphasic taxonomy, draft-whole genome sequence Abbreviations: ANI, Average Nucleotide Identity; A2pm, diaminopimelic acid, BLAST, Basic Local Alignment Search Tool; -
Clinical and Epidemiological Features
P0506 Paper Poster Session III Nontuberculous mycobacteria Nontuberculous mycobacteria in a third level hospital in Spain: clinical and epidemiological features G. Barbeito Castiñeiras1, M. Otero1, L. Ferreiro1, R. Trastoy1, J.J. Costa1, V. Tuñez1, M.L. Pérez del Molino1 1Clinical Microbiology Department- Complexo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, Spain INTRODUCTION In the last few years, we have been attending to an increasing number of isolations of non-tuberculous mycobacteria (NTM) in the health area of Santiago de Compostela (458.759 inhabitants). Our objective is to study the epidemiology of those infections caused by NTM, their associated factors and their clinical significance. METHOD Retrospective study of NTM isolations carried out from 2005 to 2013. Data sources: Microbiology Information System (OpenLab) and the electronic clinical history of Galicia (IANUS). Statistical analysis: SPSSv.20. Microbiological techniques: auramine staining, and the growth in liquid media (MGIT, Bactec 960, Becton Dickinson) 45 days and solid culture of Coletsos ® 8 weeks. Identification:phenotypic and genotypic methods: GenoType®Mycobacterium CM/AS (Hain Lifescience). For diagnosis, the criteria from the American Thoracic Society / Infectious Diseases Society of America (ATS/IDSA) 2007 were applied and the revision of the clinical history was used for the evaluation of clinical significance. RESULTS During those 9 years of study, a total of 456 strains were aisolated (Mycobacterium avium complex 34,65%, Mycobacterium intracellulare 20,83%, Mycobacterium xenopi 11,84%, Mycobacterium abscessus 9,21%, others 23,47%), concerning 212 patients. 91 patients fulfilled the NTM disease criteria of the ATS/IDSA (19,96%). The average age was 61 (range 1-89), 61,54% were male. -
View Covering the Agent and the Disease It Causes in Fish and Humans
Gcebe et al. BMC Microbiology (2018) 18:32 https://doi.org/10.1186/s12866-018-1177-9 RESEARCH ARTICLE Open Access Non-tuberculous Mycobacterium species causing mycobacteriosis in farmed aquatic animals of South Africa Nomakorinte Gcebe1* , Anita L. Michel2 and Tiny Motlatso Hlokwe1 Abstract Background: Mycobacteriosis caused by non-tuberculous mycobacteria (NTM), is among the most chronic diseases of aquatic animals. In addition, fish mycobacteriosis has substantial economic consequences especially in the aquaculture and fisheries industry as infections may significantly decrease production and trade. Some fish NTM pathogens are highly virulent and zoonotic; as such, infection of aquaria with these pathogens is a public health concern. In this study, we report isolation of nine different NTM species from sixteen aquatic animals including different fish species, frogs and a crocodile. Given the clinical significance of Mycobacterium marinum and its close relation to Mycobacterium tuberculosis, as well as the significance of ESAT 6 and CFP-10 secretion in mycobacterial virulence, we analysed the esxA and esxB nucleotide sequences of M. marinum isolates identified in this study as well as other mycobacteria in the public databases. Results: Mycobacterium shimoidei, Mycobacterium marinum, Mycobacterium chelonae, Mycobacterium septicum /M. peregrinum and Mycobacterium porcinum were isolated from gold fish, Guppy, exotic fish species in South Africa, koi and undefined fish, Knysna seahorse, as well Natal ghost frogs respectively, presenting tuberculosis like granuloma. Other NTM species were isolated from the studied aquatic animals without any visible lesions, and these include Mycobacterium sp. N845 T, Mycobacterium fortuitum, a member of the Mycobacterium avium complex, and Mycobacterium szulgai. Phylogenetic analysis of mycobacteria, based on esxA and esxB genes, separated slow growing from rapidly growing mycobacteria as well as pathogenic from non-pathogenic mycobacteria in some cases. -
Frequency and Clinical Implications of the Isolation of Rare Nontuberculous Mycobacteria
Kim et al. BMC Infectious Diseases (2015) 15:9 DOI 10.1186/s12879-014-0741-7 RESEARCH ARTICLE Open Access Frequency and clinical implications of the isolation of rare nontuberculous mycobacteria Junghyun Kim1, Moon-Woo Seong2, Eui-Chong Kim2, Sung Koo Han1 and Jae-Joon Yim1* Abstract Background: To date, more than 125 species of nontuberculous mycobacteria (NTM) have been identified. In this study, we investigated the frequency and clinical implication of the rarely isolated NTM from respiratory specimens. Methods: Patients with NTM isolated from their respiratory specimens between July 1, 2010 and June 31, 2012 were screened for inclusion. Rare NTM were defined as those NTM not falling within the group of eight NTM species commonly identified at our institution: Mycobacterium avium, M. intracellulare, M. abscessus, M. massiliense, M. fortuitum, M. kansasii, M. gordonae, and M. peregrinum. Clinical, radiographic and microbiological data from patients with rare NTM were reviewed and analyzed. Results: During the study period, 73 rare NTM were isolated from the respiratory specimens of 68 patients. Among these, M. conceptionense was the most common (nine patients, 12.3%). The median age of the 68 patients with rare NTM was 68 years, while 39 of the patients were male. Rare NTM were isolated only once in majority of patient (64 patients, 94.1%). Among the four patients from whom rare NTM were isolated two or more times, only two showed radiographic aggravation caused by rare NTM during the follow-up period. Conclusions: Most of the rarely identified NTM species were isolated from respiratory specimens only once per patient, without concomitant clinical aggravation. -
INFECTIOUS DISEASE Short Title: Disseminated Mycobacteriosis In
INFECTIOUS DISEASE Short Title: Disseminated Mycobacteriosis in Cats Non-tuberculous Mycobacteria can Cause Disseminated Mycobacteriosis in Cats H. Pekkarinen,⃰ N. Airas⃰ , L. E. Savolainen†, M. Rantala‡, S. Kilpinen‡, O. Miuku‡, M. Speeti§, V. Karkamo¶, S. Malkamäki*, M. Vaara†, A. Sukura⃰ and P. Syrjä* ⃰ Department of Veterinary Biosciences, Faculty of Veterinary Medicine, PO Box 66, University of Helsinki, †Department of Clinical Microbiology, University of Helsinki and Helsinki University Hospital, HUSLAB, Helsinki, Finland, ‡Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, PO Box 57, University of Helsinki, §Herttoniemi Veterinary Clinic, Hiihtomäentie 35, Helsinki and ¶Pathology Research Unit, Finnish Food Safety Authority Evira, Mustialankatu 3, Helsinki, Finland. Correspondence to: H. Pekkarinen (e-mail: [email protected]). Summary Mycobacteriosis caused by non-tuberculous mycobacteria (NTM) is a rising concern in human medicine both in immunocompromised and immunocompetent patients. In cats, mycobacteriosis caused by NTM is considered mostly to be a focal or dermal infection, with disseminated disease mostly caused by Mycobacterium avium. We describe three cases of disseminated mycobacteriosis in cats, caused by M. malmoense, M. branderi/shimoidei and M. avium, with no identified underlying immunosuppression. In all cases, extracellular mycobacteria were seen in the pulmonary epithelium, intestinal lumen and glomerular tufts, which could affect the shedding of the organism. The present study highlights the importance of mycobacteriosis as a differential even in immunocompetent animals. Considering the close relationship of owners and pets and the potential presence of free mycobacteria in secretions, cats should be considered as a possible environmental reservoir for mycobacteria. Keywords: mycobacteriosis; cat; non-tuberculous mycobacteria Introduction Mycobacteria are acid-fast, aerobic, non-spore forming rod shaped bacteria that range from obligate pathogens to environmental saprophytes. -
Identification of Mycobacterium Species Following Growth Detection
International Journal of Mycobacteriology xxx (2014) xxx– xxx Available at www.sciencedirect.com ScienceDirect journal homepage: www.elsevier.com/locate/IJMYCO Identification of Mycobacterium species following growth detection with the BACTEC MGIT 960 system by DNA line probe assay Kee Peng Ng *, Devi Velayuthan Rukumani, Jennifer Chong, Harvinder Kaur Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 59400 Kuala Lumpur, Malaysia ARTICLE INFO ABSTRACT Article history: Background: The tuberculosis and infections caused by nontuberculous mycobacterial Received 25 March 2014 (NTM) species are increasing in patients presented with respiratory illness, and it is crucial Accepted 31 March 2014 to document the epidemiology of these infections. Available online xxxx Objectives: To study the mycobacterial species and in vitro drug susceptibility trends of Mycobacterium tuberculosis found in the respiratory specimens. Keywords: Materials and methods: A prospective descriptive study from July 2009 to December 2012. Mycobacterium tuberculosis The BACTEC MGIT system tubes with growth were used in the study. GenoType Mycobacte- Nontuberculous mycobacterial rium (Hain Diagnostika, Nehren, Germany) assays were used to identify the mycobacteria. species The drug susceptibility testing was performed by the MGIT 960 system. BACTEC MGIT system Results: A total of 1745 MGIT 960 system positive tubes were included. M. tuberculosis com- GenoType Mycobacterium assays plex (MTC) constituted 67.45% of the yield isolated, 30.83% were