Lymphatic Cells Normal Lymphocyte

Lymphatic Cells

Normal lymphocyte Prolymphocyte Binucleated lymphocyte Villous lymphocyte

Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio high medium/high high medium/high Nucleus shape Nucleus shape Nucleus shape Nucleus shape oval round/oval binucleated round/oval Nuclear chromatin Nuclear chromatin Nuclear chromatin Nuclear chromatin heterogeneous, clumped intermediate, always with clumped condensed nucleolus Cytoplasm Cytoplasm Cytoplasm narrow, basophilic Cytoplasm narrow, basophilic lightly basophilic, short villi, lightly basophilic unipolar

Lymphoid cell Large granulated lymphocyte (LGL) Lymphoplasmocytic cell Plasma cell

Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio medium medium medium low/medium Nucleus shape Nucleus shape Nucleus shape Nucleus shape round/oval oval oval round/oval, eccentric localisation Nuclear chromatin Nuclear chromatin Nuclear chromatin Nuclear chromatin intermediate condensed condensed clumped Cytoplasm Cytoplasm Cytoplasm Cytoplasm lightly basophilic, diffluent, lightly basophilic, azurophilic abundant, stronger basophilic deeply basophilic, perinuclear often around red blood cells granules clear zone (Golgi apparatus)

Monocytoid lymphocyte Hairy cell Centrocyte Centroblast

Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio medium low/medium medium/high medium/variable Nucleus shape Nucleus shape Nucleus shape Nucleus shape lobed, indented oval/kidney-shaped round/oval, notched round/oval Nuclear chromatin Nuclear chromatin Nuclear chromatin Nuclear chromatin intermediate intermediate condensed fine, nucleoli at nuclear membrane Cytoplasm Cytoplasm Cytoplasm abundant, moderately basophilic lightly basophilic, circular hairy basophilic, sparse Cytoplasm projections moderately basophilic

T lymphocyte Sézary cell Activated lymphocyte Immunoblast

Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio high medium medium/variable medium/variable Nucleus shape Nucleus shape Nucleus shape Nucleus shape irregular, lobed cerebriform oval-indented round/oval Nuclear chromatin Nuclear chromatin Nuclear chromatin Nuclear chromatin clumped intermediate intermediate fine, central nucleoli Cytoplasm Cytoplasm Cytoplasm Cytoplasm narrow, basophilic basophilic light, basophilic rim moderately to deeply basophilic

Highly malignant lymphoma cell Lymphoblast Lymphoblast with L3 morphology Myeloma cell

Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio medium/variable high high medium Nucleus shape Nucleus shape Nucleus shape Nucleus shape round/oval round/oval round/oval like plasma cell Nuclear chromatin Nuclear chromatin Nuclear chromatin Nuclear chromatin uniformly dense smooth, fine smooth, fine intermediate, with or without nucleoli Cytoplasm Cytoplasm Cytoplasm moderately to deeply basophilic basophilic, variable strongly basophilic, Cytoplasm like plasma cell, vacuoles Golgi zone may be absent

Lymphocyte/plasma cell Mott cell Hodgkin cell Sternberg-Reed cell with Dutcher bodies Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio Nuclear-cytoplasmic ratio low/medium low/medium medium up to < 50 % medium up to < 50 % Nucleus shape Nucleus shape round/oval Nucleus shape Nucleus shape round/oval, eccentric localisation single nucleus in a large cell like Hodgkin cell but multiple Nuclear chromatin nuclei in a very large cell Nuclear chromatin condensed, one to several intra- Nuclear chromatin clumped nuclear light blue immunoglobu- intermediate, large nucleolus Nuclear chromatin lin inclusions (Dutcher bodies) reticular Cytoplasm basophilic, light Cytoplasm blue clustered immunoglobulin Cytoplasm like plasma cell or lightly basophilic Cytoplasm inclusions (Russell bodies) lymphoplasmocytic cell lightly to moderately basophilic

Lymphocytosis, reactive, neoplastic B lymphocyte, T lymphocyte, cytology Lymphoma, cytology

A variety of different types of lymphocytes with the presence of lympho With the exception of NK cells (natural killer cells) the lineage of lym- Indolent lymphomas are morphologically very heterogeneous, but the cells plasmocytic cells and activated lymphocytes suggests a reactive cause. phocytes cannot be reliably determined with a Pappenheim stain, but have one thing in common: The nuclear chromatin is stained unevenly; In contrast, a clonal, neoplastic cell population is predominantly mono some observations can be made. B lymphocytes, for example, do not show apart from clumped, condensed looking, dark areas there are light areas morphic. In children and young adults, a lymphocytosis is frequently due azurophilic granulation. T lymphocytes usually have an irregular nuclear with low chromatin density. Nucleoli may be present. to an infection. In elderly people, a neoplastic aetiology is more likely. shape and may show fine-grained azurophilic granules. Aggressive lymphomas: medium to large cells, chromatin evenly distrib- uted, no clumping. Presence of nucleoli is frequent. Cytoplasm narrow, strongly basophilic.

Sysmex Europe GmbH Bornbarch 1, 22848 Norderstedt, Germany · Phone +49 40 52726-0 · Fax +49 40 52726-100 · [email protected] · www.sysmex-europe.com

In cooperation with: Universitätsklinikum Aachen Klinik für Onkologie/Hämatologie und Stammzelltransplantation · Pauwelsstraße 30, 52074 Aachen, Germany · Telefon +49 241 80-0 · [email protected] · www.ukaachen.de Please find your local Sysmex representative address under www.sysmex-europe.com