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Supplementary Information Supplementary Information A first higher-level time-calibrated phylogeny of antlions (Neuroptera: Myrmeleontidae) Bruno Michel, Anne-Laure Clamens, Olivier Béthoux, Gael J. Kergoat, Fabien L. Condamine Table S1. Taxon sampling used in this study. It contains information on the taxonomy and systematics, as well as the voucher ID, and the collection locality. It also contains the GenBank accession numbers for each molecular marker successfully sequenced. Table S2. PCR conditions (a) and PCR primers (b) used in this study to sequence the selected genes. Figure S1. The Bayesian consensus tree inferred with MrBayes on the 113-taxa and seven genes. Posterior probabilities depict node supports. Figure S2. Bayesian time-calibrated tree as inferred with BEAST (three fossil calibrations set with uniform priors, and a birth-death process a the tree prior). Figure S3. Bayesian time-calibrated tree as inferred with BEAST (four fossil calibrations set with uniform priors, and a birth-death process a the tree prior). ! ! Table S1. Taxon sampling used in this study. It contains information on the taxonomy and systematics, as well as the voucher ID, and the collection locality. It also contains the GenBank accession numbers for each molecular marker successfully sequenced. Voucher Family Subfamily Tribe Subtribe Genus Species Locality COI COIII Cytb 12S 16S 18S 28S Ascalaphidae Ascalohybris subjacens - NC_021428 NC_021428 NC_021428 NC_021428 NC_021428 KC413913 - Ascalaphidae Ascaloptynx appendiculata - NC_011277 NC_011277 NC_011277 NC_011277 NC_011277 - - Ascalaphidae Libelloides macaronius - NC_015609 NC_015609 NC_015609 NC_015609 NC_015609 - - Ascalaphidae Libelloides rhomboideus - EU839736 - - - EU734868 EU815250 - Ascalaphidae Melambrotus papio - - AY620088 - - AY620176 AY620030 - Ascalaphidae Neomelambrotus molestus - JX294184 - - JX294125 JX294239 - BrM065 Myrmeleontidae Myrmeleontinae Acanthaclisini Centroclisis brachygaster Kuzikus Submitted - Submitted Submitted - - - BrM064 Myrmeleontidae Myrmeleontinae Acanthaclisini Centroclisis punctulata Bandiagara Submitted - Submitted Submitted Submitted - - BrM067 Myrmeleontidae Myrmeleontinae Acanthaclisini Centroclisis rufescens Parc W Submitted - Submitted Submitted Submitted - - BrM068 Myrmeleontidae Myrmeleontinae Acanthaclisini Centroclisis ustulata Bobo Dioulasso Submitted - Submitted Submitted Submitted - Submitted BrM069 Myrmeleontidae Myrmeleontinae Acanthaclisini Fadrina rufa Bobo Dioulasso - - Submitted - Submitted - - BrM070 Myrmeleontidae Myrmeleontinae Acanthaclisini Jaya atrata Bobo Dioulasso Submitted - - Submitted - - - BrM071 Myrmeleontidae Myrmeleontinae Acanthaclisini Phanoclisis longicollis Sadore - - Submitted Submitted Submitted - Submitted BrM072 Myrmeleontidae Myrmeleontinae Acanthaclisini Syngenes debilis Sadore Submitted - Submitted Submitted Submitted - - Myrmeleontidae Myrmeleontinae Brachynemurini Brachynemurus sackeni - HQ386921 - - - - - - Myrmeleontidae Myrmeleontinae Brachynemurini Brachynemurus abdominalis - GU013631 - - - - - - Myrmeleontidae Myrmeleontinae Brachynemurini Brachynemurus hubbardi - HQ386930 - - - - - - Myrmeleontidae Myrmeleontinae Brachynemurini Scotoleon yavapai - HQ386931 - - - - - - Myrmeleontidae Myrmeleontinae Dendroleontini Epacanthaclisis banksi - NC_025905 NC_025905 NC_025905 NC_025905 NC_025905 - - BrM012 Myrmeleontidae Myrmeleontinae Gepini Gepus invisus Bouanane Submitted - - Submitted Submitted - - BrM079 Myrmeleontidae Myrmeleontinae Gepini Solter francoisi Missour ECWP Submitted - Submitted Submitted Submitted - - BrM078 Myrmeleontidae Myrmeleontinae Gepini Solter pr. liber Missour ECWP Submitted - Submitted Submitted Submitted - - BrM007 Myrmeleontidae Myrmeleontinae Gepini Solter liber Missour ECWP Submitted - - Submitted Submitted - Submitted BrM080 Myrmeleontidae Myrmeleontinae Myrmecaelurini Lopezus sp. Aftout Fay Submitted - Submitted - Submitted - - BrM074 Myrmeleontidae Myrmeleontinae Myrmecaelurini Myrmecaelurus lepidus Figuig Submitted - - - Submitted - Submitted BrM049 Myrmeleontidae Myrmeleontinae Myrmecaelurini Myrmecaelurus sectorius Sadore Submitted - - Submitted Submitted - - Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Euroleon nostras - KF289782 KF289802 - - KF321978 KF322015 KF321997 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Hagenomyia tristis - KF289778 KF289797 - - KF366491 KF366495 KF366493 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Megistoleon ritsemae - KF963279 KF289798 - - KF366492 KF366496 KF366494 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon alternans - KC182624 - - - - - KC312264 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon bore - KC182622 KC312270 - - KC195845 KC312193 KC312263 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon caliginosus - JQ864074 KC312284 - - KC195854 KC312191 KC312231 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon crudelis - KF748927 - - - KF748922 - KF748924 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon fasciatus - KF289773 KF289792 - - KF321971 KF322008 KF321989 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon formicarius - KF289780 KF289800 - - KF321976 KF322013 KF321995 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon gerlindae - JQ864072 KC312303 - - KC195828 KC312214 KC312236 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon immaculatus - FJ207458 FJ207458 FJ207459 FJ207458 - U65137 U65191 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon immanis KM216750 KM216750 KM216750 KM216750 KM216750 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon inconspicuus - JQ864064 KC312282 - - KC195842 KC312181 KC312246 BrM061 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon lethifer Folonzo Submitted - - - - - - Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon mariaemathildae - KC182596 KC312286 - - KC195852 KC312187 KC312259 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon obscurus - KF289775 KF289794 - - KF321973 KF322010 KF321991 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon punicanus - JQ864075 KC312306 - - KC195829 KC312212 KC312232 BrM051 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon doralice Kuzikus Submitted - Submitted Submitted Submitted - Submitted BrM008 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon hyalinus Missour ECWP - - Submitted Submitted Submitted - Submitted BrM052 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon obscurus Kuzikus Submitted - - Submitted - - BrM010 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon sp. Ouled Ali Submitted - Submitted Submitted Submitted - Submitted Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon alcestris - KF289776 KF289795 - - - - KF321992 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon carolinus - KF748928 - - - KF748923 KF748926 KF748925 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon cf. noacki - KC182611 KC312310 - - KC195825 KC312208 KC312239 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon pr. obscurus - KF289774 KF289793 - - KF321972 KF322009 KF321990 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon quinquemaculatus - KF289777 KF289796 - - KF321974 KF322011 KF321993 Myrmeleontidae Myrmeleontinae Myrmeleontini Myrmeleontina Myrmeleon sp. - KC182615 KC312283 - - KC195853 KC312192 KC312254 BrM044 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Banyutus guttifer Sikasso Submitted - - - - - - BrM043 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Banyutus striolatum Folonzo Submitted - - - Submitted - - BrM006 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Creoleon aegyptiacus Missour ECWP Submitted - Submitted Submitted Submitted - Submitted BrM055 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Creoleon africanus Farako Submitted - Submitted - - - - BrM056 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Creoleon diana Kuzikus Submitted - Submitted Submitted Submitted - Submitted BrM058 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Creoleon lugdunensis Safi Submitted - Submitted Submitted Submitted - - BrM011 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Creoleon sp. Enjil ECWP Submitted - Submitted Submitted Submitted - Submitted BrM001 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Distoleon annulatus Enjil ECWP Submitted - Submitted Submitted Submitted - Submitted BrM014 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Distoleon divisus Ouled Ali Submitted - Submitted Submitted Submitted - Submitted BrM035 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Distoleon harpalyce Farako Submitted - - - - - - BrM033 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Distoleon tetragrammicus Assas Submitted - Submitted Submitted Submitted - - BrM075 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Geyria lepidula Inchiri Submitted - - - - - - BrM048 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Macronemurus euanthe Kuzikus Submitted - Submitted Submitted Submitted - Submitted BrM009 Myrmeleontidae Myrmeleontinae Nemoleontini Neuroleontina Macronemurus gallus Ouled Ali Submitted - - - - - Submitted BrM041 Myrmeleontidae Myrmeleontinae Nemoleontini Nemoleontina Nemoleon filiformis Bamako Submitted - Submitted - - - - BrM039
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