Downloaded from rnajournal.cshlp.org on September 24, 2021 - Published by Cold Spring Harbor Laboratory Press APOBEC1 complementation factor (A1CF) and RBM47 interact in tissue-specific regulation of C to U RNA editing in mouse intestine and liver Valerie Blanc1, Yan Xie1, Susan Kennedy1, Jesse D. Riordan2, Deborah C. Rubin1, Blair B. Madison1, Jason C. Mills1, Joseph H. Nadeau2, Nicholas O. Davidson1,3 1Division of Gastroenterology, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63105, 2Pacific Northwest Research Institute, Seattle, WA 98122 3To whom communication should be addressed: Email:
[email protected] Running title: A1CF and RBM47 interactively regulate C to U RNA editing Keywords: APOBEC1; A1CF; RBM47; ApoB; Intestine; Liver Downloaded from rnajournal.cshlp.org on September 24, 2021 - Published by Cold Spring Harbor Laboratory Press ABSTRACT (246 words) Mammalian C to U RNA is mediated by APOBEC1, the catalytic deaminase, together with RNA binding cofactors (including A1CF and RBM47) whose relative physiological requirements are unresolved. Although A1CF complements APOBEC1 for in-vitro RNA editing, A1cf–/– mice exhibited no change in apolipoproteinB (apoB) RNA editing, while Rbm47 mutant mice exhibited impaired intestinal RNA editing of apoB as well as other targets. Here we examined the role of A1CF and RBM47 in adult mouse liver and intestine, following deletion of either one or both gene products and also following forced (liver or intestinal) transgenic A1CF expression. There were minimal changes in hepatic and intestinal apoB RNA editing in A1cf–/– mice and no changes in either liver- or intestine-specific A1CF transgenic mice. Rbm47 liver-specific knockout (Rbm47LKO) mice demonstrated reduced editing in a subset (11 of 20) RNA targets, including apoB.