Histone H2B, NT (HIST1H2BB, H2BFF, HIST1H2BE, H2B type 1-B, Histone H2B.1, Histone H2B.f) (Azide free) (HRP)

Catalog number 036608-HRP Supplier United States Biological

Histones are basic nuclear that are responsible for the structure of the chromosomal fiber in . consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core (H2A, H2B, H3, and H4). The fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on 6p22-p21.3. Applications Suitable for use in Western Blot, ELISA Recommended Dilution ELISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note Applications are based on unconjugated antibody. Immunogen HIST1H2BB/HIST1H2BE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BB/HIST1H2BE. Formulation Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP). Purity Purified by A affinity chromatography. Specificity Human Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications E WB Crossreactivity Hu Storage -20°C Detection Method HRP Reference Kim, S.C., et al. Mol. Cell 23(4):607-618(2006) Pavri, R., et al. Cell 125(4):703-717(2006) Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006) Zhu, B., et al. Mol. Cell 20(4):601-611(2005) Golebiowski, F., et al. Mol. Cell. Biochem. 279 (1-2), 133-139 (2005) :

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