Multiscale Biomimetic Approaches for Regulation of Cell Function and Tissue Regeneration

Jangho Kim, Ph.D. Chonnam National University

Living cells are exposed to complex and functional microenvironment including soluble macromolecules, biophysical cues, and interactions between cell-cell and cell-extracellular matrix (ECM). This suggests that the design and manipulation of engineered cellular microenvironments is of great important in a wide variety of biological applications such as fundamental biology, therapeutic and diagnostic research, stem cells and regenerative medicine, and developing in vitro disease models. Compared with the effects of soluble macromolecules and biophysical cues, the extent and importance of architecture of ECMs or tissues in defining cellular function are currently poorly understood. Namely, the architecture effects of ECMs or specific tissues are mainly neglected or minimized as a considering factor for most in vitro and in vivo experimentation. In this talk, I will present multidisciplinary efforts directed towards the development of multiscale engineered biomimetic systems for biomedical applications. Specifically, I will talk about i) our current efforts on developing a series of advanced multiscale patterned platforms inspired by the unique architectures of native tissues and ECMs that can provide cells the in vivo-like topographical cell environment, and ii) their applications for better understanding of the function of living cells and tissue regeneration without complex surgical treatments or tissue transplantation in combination with traditional molecular and cell biology approaches.

Publications Related in This Talk 1) J. Kim et al., Biologically inspired micro- and nanoengineering systems for functional and complex tissues. Tissue Engineering Part A 2014:20:2127-2130. 2) J. Kim et al., Multiscale patterned transplantable stem cell patches for bone tissue regeneration. Biomaterials 2014:35:9058-9067. 3) J. Kim et al., Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells. Scientific Reports 2013:3. 4) J. Kim et al., Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells. Biomaterials 2013:34:7257-7268. 5) J. Kim et al., Charged nanomatrices as efficient platforms for modulating cell adhesion and shape. Tissue Engineering Part C: Methods 2012:18(12):913-923 (selected as a cover article and a featured article).

Jangho Kim, Ph.D. CURRICULUM VITAE Assistant Professor Phone: +82-10-5389-5494 Nanoengineered Biomaterial Systems Lab. E-mail: [email protected] Chonnam National University http://openwetware.org/wiki/Jangho_Kim

EDUCATION AND EMPLOYMENT 3/2015 – present Chonnam National University Assistant Professor in Department of Rural and Biosystems Engineering 9/2014 – 2/2015 Seoul National University Senior Researcher in Research Institute of Agriculture and Life Sciences 9/2012 – 8/2014 Seoul National University Ph.D. in Biosystems Engineering (Advisor: Prof. Jong Hoon Chung) 11/2010 – 8/2012 Seoul National University Research Associate in Research Institute of Agriculture and Life Sciences 3/2009 – 10/2010 University of Illinois at Urbana-Champaign (UIUC) Research Scholar in Department of Electrical and Computer Engineering (Advisor: Prof. Kevin Kim) 3/2007 – 2/2009 Seoul National University M.S. in Biosystems Engineering (Advisor: Prof. Jong Hoon Chung) 3/2005 – 2/2007 Seoul National University Exchange B.S. Student and Researcher in Department of Biosystems & Biomaterials Science and Engineering (Advisor: Prof. Jong Hoon Chung) 3/2000 – 8/2006 Chonnam National University B.S. in Biosystems Engineering

HONORS AND AWARDS 2014 Keynote Speaker, TERM STEM 2014, Porto, Portugal 2014 Samsung HumanTech Paper Award, Samsung Electronics 2013 Highlighted in Nanomedicine, Synergistic effects of nanotopography and co- culture with endothelial cells on osteogenesis of mesenchymal stem cells, Biomaterials 34:7257-7268, 2013 2013 Young Plenary Speaker, Tissue Engineering & Regenerative Medicine International Society Asia-Pacific (TERMIS-AP) Conference, Shanghai, P.R. China 2013 Invited Special Speech, Tissue Engineering & Regenerative Medicine International Society Asia-Pacific (TERMIS-AP) Conference, Shanghai, P.R. China 2013 Best Presentation Award, Annual Meeting of Korea Society for Stem Cell Research 2013 Outstanding Research Award, Korean Tissue Engineering and Regenerative Medicine Society 2013 Best Oral Presentation Award, Korea Biosystems Engineering Spring Conference 2013 Selected Special Issue Topic in Tissue Engineering Parts A, B, & C - Jangho Kim - CV 1/11 update 2015-05-27

Bioinspired Micro- and Nanoengineered Platforms for Tissue Engineering-, Tissue Engineering Parts A, B, & C journals 2013 Selected as One of Most Read Articles (2/2013), Graphene-incorporated chitosan substrata for promoting adhesion and differentiation of stem cells, Journal of Materials B (as Communication) 2013:1:933-938. 2013 Selected as Cover Article, Bioactive effects of graphene oxide cell culture substratum on structure and function of human adipose-derived stem cells, Journal of Biomedical Materials Research: Part A 2013:101A:3520-3530 2012 - 2013 Biosystems Engineering Foundation Graduate Fellowship, Seoul National University 2012 Selected as Cover Article, Featured Article, and Most Read Articles (1/2013- current), Charged nanomatrices as efficient platforms for modulating cell adhesion and shape, Tissue Engineering Part C: Methods, 2012:18(12):913-923 2012 Young Scientist Award, Korean and Japan Society for Biomaterials, the joint meeting of the Korean and Japanese Society for Biomaterials 2012 Tissue Engineering Young Investigator Council, Tissue Engineering & Regenerative Medicine International Society and Tissue Engineering Parts A, B, & C journals (selected from the researchers of over 300 qualified candidates; the two representatives from Asia) 2012 Outstanding Research Award, Korean Tissue Engineering and Regenerative Medicine Society 2012 Best Poster Presentation Award, Korea Biosystems Engineering Winter Conference 2011 Excellent Researcher Award, Seoul National University 2008 Best Poster Presentation Award, Korea Biosystems Engineering Conference 2007 - 2008 National Graduate S&T Scholarship, Korea Science and Engineering Foundation 2007 Best Poster Presentation Award, Korea Biosystems Engineering Conference 2005 Selected as One of the Representative Students in Korea for Participating in the Science Rover to China, Ministry of Education of Korea

PROFESSIONAL ACTIVITIES AND SERVICES Associate Editor, Engineering in Agriculture, Environment and Food. 2015 – Current Tissue Engineering Young Investigator Council (YIC) member (Editorial Board), Tissue Engineering Parts A, B, & C journals. 2012-2013 Theme Co-Organization for Tissue Engineering YIC Special Issue, Tissue Engineering Parts A, B, & C journals. 2013 Session Chair, Tissue Engineering & Regenerative Medicine International Society Asia-Pacific (TERMIS-AP) Conference, Shanghai, P.R. China, 2013 Program Committee Member as the Judge for SYIS Best Oral and Poster Competition, Tissue Engineering & Regenerative Medicine International Society Asia-Pacific (TERMIS-AP) Conference, Shanghai, P.R. China, 2013 Jangho Kim - CV 2/11 update 2015-05-27

Professional Societies: Korea Society for Agriculture Machinery, Korean Society for Biomaterials, Korean Tissue Engineering and Regenerative Medicine Society, Korean Society for Stem Cell Research, American Society of Agricultural and Biological Engineers, Tissue Engineering & Regenerative Medicine International Society Journal Reviews: Advanced Functional Materials (Wiley), Archives of Medical Research (Elsevier), Biomacromolecules (ACS publications), Chemical Communications (RSC publishing), Egyptian Journal of Basic and Applied Sciences (Elsevier), & Environmental Science (RSC publishing), General Physiology and Biophysics (AEPress), Integrative Biology (RSC publishing), Materials Science and Engineering C (Elsevier), RSC Advances (RSC publishing), Scientific Reports (Nature Publishing), Journal of Materials Chemistry (RSC publishing), Materials Letters (Elsevier), Journal of Materials Chemistry B (RSC publishing), Lab on a Chip (RSC publishing), Tissue Engineering (Mary Ann Liebert, Inc), Tissue Engineering and Regenerative Medicine (Springer)

PATENTS 1. Y.H. Choung, J.H. Chung, J. Kim, P.H. Choung. Artificial Chitosan Patch for Eardrum Regeneration. Issued (10107041)

PUBLICATIONS A. Thesis: J. Kim, “Nanoengineered Biomimetic Platforms for Controlling Cell Function and Tissue Regeneration,” Ph.D. Thesis, Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Republic of Korea, 2014 (Advisor: Prof. Jong Hoon Chung) J. Kim, “Development of Tissue Engineered Artificial Eardrums,” M.S. Thesis, Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Republic of Korea, 2009 (Advisor: Prof. Jong Hoon Chung)

B. Journal Publications: Peer Reviewed Journal Publications as a Main Author [Published] 1. S.W. Kim*, J. Kim*, H. Seonwoo, K.J. Jang, Y.J. Kim, J.H. Chung, Y.H. Choung. Latent progenitor cells as potential regulators for tympanic membrane regeneration. Scientific Reports (in press). [*co-first author] [SCIE, IF: 5.078, JCR: 9.1%] 2. J. Kim, S.B. Park, Y. Kim, K.T. Lim, H. Seonwoo, Y.H. Choung, B.H. Hong, J.H. Chung. Monolayer graphene- directed growth and neuronal differentiation of mesenchymal stem cells. Journal of Biomedical Nanotechnology (in press) [SCI, IF: 7.578, JCR: 6.3%] 3. J. Kim, Y. Kim, W.G. Bae, K.J. Jang, Y.H. Choung, J.H. Chung. Topographical extracellular matrix cues on anticancer drug-induced cytotoxicity in stem cells. Journal of Biomedical Materials Research: Part B - Applied Biomaterials (in press) [SCI, IF: 2.328, JCR: 36.4%] 4. S. Oh*, J. Kim*, H.R. Ryu, K.T. Lim, J.H. Chung, N.L. Jeon. Design, fabrication, and application of a Jangho Kim - CV 3/11 update 2015-05-27

microfluidic device for investigating physical stress-induced behavior of yeast and microalgae. Journal of Biosystems Engineering 2014:39:244-252. [*co-first author] 5. J. Kim, W.G. Bae, H.W. Choung, K. T. Lim, H. Seonwoo, H. E. Jeong, K.Y. Suh, N. L. Jeon, P.H. Choung, J.H. Chung. Multiscale patterned transplantable stem cell patches for bone tissue regeneration. Biomaterials 2014:35:9058-9067. (Samsung HumanTech Paper Award from the Samsung Electronics, 2014; Selected as ‘This Week’s Top Story’ from the Extracellular Matrix News, 2014) [SCI, IF: 8.312, JCR: 3.1%] 6. J. Kim, W.G. Bae, K.T. Lim, K.J. Jang S. Oh, K.J. Jang, N.L. Jeon, K.Y. Suh, J.H. Chung. Density of nanopatterned surfaces for designing bone tissue engineering scaffolds. Materials Letters 2014:130:227-231. [SCI, IF: 2.269, JCR: 24.4%] 7. J. Kim#, H.N. Kim, Y. Lang, A. Pandit#. Biologically inspired micro- and nanoengineering systems for functional and complex tissues. Tissue Engineering: Part A 2014:20:2127-2130. [#corresponding authors] (Invited as Inaugural Young Investigator Council Special Issue) [SCI, IF: 4.254, JCR: 13.9%] 8. J. Kim, H.N. Kim, K.T. Lim, Y. Kim, H. Seonwoo, S.H. Park, H.J. Lim, D.H. Kim, P.H. Choung, Y.H. Choung, K.Y. Suh, J.H. Chung. Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells. Scientific Reports 2013:3: doi:10.1038/srep03552. [a new multidisciplinary science journal from the publishers of Nature] (Young Scientist Award from the Korean and Japanese Society for Biomaterials, 2012 and Award for Young Plenary Speaker from TERMIS-AP Conference, 2013) [Press released in major media such as Dong-A Ilbo, YTN, YTN science. Selected in the top 5% article ranked by Altmetric] [SCIE, IF: 5.078, JCR: 9.1%] 9. J. Kim, H.N. Kim, K.T. Lim, Y. Kim, S. Pandey, P. Garg, Y.H. Choung, P.H. Choung, K.Y. Suh, J.H. Chung. Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells Biomaterials 2013:34:7257-7268. (Outstanding Research Award from the Korean Tissue Engineering and Regenerative Medicine Society, 2013; Highlighted in Nanomedicine 2013:8(11):1743-1749, 2013) [SCI, IF: 8.312, JCR: 3.1%] 10. A.L. Im*, J. Kim*, K.T. Lim, H. Seonwoo, W.J. Cho, P.H Choung, J.H. Chung. Effects of micro-electrical stimulation on regulation of behavior of electro-active stem cells. Journal of Biosystems Engineering 2013:38:113-120 [*co-first author] 11. J. Kim, S. Choi, Y. Kim, K.T. Lim, H. Seonwoo, Y. Park, D.H. Kim, P.H. Choung, C.S. Cho, S.Y. Kim, Y.H. Choung, J.H. Chung. Bioactive effects of graphene oxide cell culture substratum on structure and function of human adipose-derived stem cells, Journal of Biomedical Materials Research: Part A 2013:101A:3520-3530. (Selected as a Cover Article) [SCI, IF: 2.841, JCR: 23.4%] 12. J. Kim, Y.R. Kim, H. Seonwoo, K.T. Lim, Y. Kim, Y.H. Choung, P.H. Choung, J.H. Chung. Graphene- incorporated chitosan substrata for promoting adhesion and differentiation of stem cells, Journal of Materials Chemistry B (as Communication) 2013:1:933-938. (Outstanding Research Award from the Korean Tissue Engineering and Regenerative Medicine Society, 2012; Selected as One of the Most Read Articles (top ranked) in JMC-B in January and February, 2013) [SCI, IF: NS] 13. J. Kim, S.W. Kim, S. Park, K.T. Lim, H. Seonwoo, Y. Kim, B.H. Hong, Y.H. Choung, J.H. Chung. Bacterial cellulose nanofibrillar patch as a wound healing platform of tympanic membrane perforation, Advanced Healthcare Materials 2013:2:1525-1531. [SCIE, IF: 4.88, JCR: 15.6%] 14. J. Kim, D.H. Kim, K.T. Lim, H. Swonwoo, S.H. Park, Y.R. Kim, Y.H. Choung, P.H. Choung, J.H. Chung. Charged nanomatrices as efficient platforms for modulating cell adhesion and shape, Tissue Engineering Part C: Jangho Kim - CV 4/11 update 2015-05-27

Methods, 2012:18(12):913-923. (Selected as a Cover Article and a Featured Article) [SCI, IF: 4.254, JCR: 13.9%] 15. J. Kim, S.W. Kim, S.J. Choi, K.T. Lim, J.B. Lee, H. Seonwoo, P.H. Choung, K.H. Park, C.S. Cho, Y.H. Choung, J.H. Chung. A healing method of tympanic membrane perforations using three-dimensional porous chitosan scaffolds. Tissue Engineering: Part A, 2011:17(21-22): 2763-2772. (Excellent Researcher Award from the Seoul National University) [SCI, IF: 4.254, JCR: 13.9%] 16. J. Kim, S.J. Choi, K.T. Lim, P.H. Choung, J.H. Bae, Y.H. Choung, J.H. Chung. Tympanic membrane regeneration using a water-soluble chitosan patch. Tissue Engineering Part A, 2010:16(1): 225-232. [SCI, IF: 4.254, JCR: 13.9%] 17. J. Kim, P.H. Choung, I.Y. Kim, K.T. Lim, H.M. Son, Y.H. Choung, C.S. Cho, J.H. Chung. Electrospun nanofibers composed of poly(ε-caprolactone) and polyethylenimine for tissue engineering applications. Materials Science and Engineering C, 2009:29:1725-1731. (Selected as Top 25 Hottest Articles in Materials Science and Engineering: C (January to June 2009)) [SCIE, IF: 2.736, JCR: 46.9%] 18. J. Kim, J.H. Bae, K.T. Lim, P.H. Choung, J.S. Park, S.J. Choi, A.L. Im, E.T. Lee, Y.H. Choung, J.H. Chung. Development of water-insoluble chitosan patch scaffold to repair traumatic tympanic membrane perforations. Journal of Biomedical Materials Research Part A, 2009:90:446-455. [SCI, IF: 2.841, JCR: 23.4%] 19. J. Kim, K.T Lim, P.H Choung, Y.H Choung, C.S Cho, J.H Chung. Mechanical stimulation of mesenchymal stem cells for tissue engineering. Tissue Engineering and Regeneration Medicine, 2009:6(1-3):199- 206. [SCIE, IF: 0.613, JCR: 89.6%] [In Progress] 20. J. Kim, P. Garg, H.N. Kim, K.J. Jang, K.T. Lim, P.H. Choung, J.H. Chung. Synergistically enhanced gene delivery by non-viral vectors on nanopatterned matrix (under review) 21. W.G. Bae*, J. Kim*, Y.H. Choung, Y. Chung, K.Y. Suh, C. Pang, J.H. Chung, H.E. Jeong. Bio-inspired configurable multiscale tissue-like structure with precisely controlled size for functional alignment and guided orientation of cells (under review). [*co-first author] 22. W.G. Bae, H.E. Jeong, J. Kim#. Fabrication of multiscale engineered hierarchical structures for bio-inspired cell culture (submitted). [#corresponding author]

Peer Reviewed Journal Publications as a Co-Author 23. S. Park*, J. Park*, I. Jom S.P. Cho, D. Sung, S. Ryu, M. Park, K.A. Min, J. Kim, S. Hong, B.S. Kim, B.H. Hong. In situ hybridization of carbon nanotubes with bacterial cellulose for three-dimensional hybrid bioscaffolds Biomaterials (accepted) 24. Y.J. Kim, S.G. Park, J. Kim, H.J. Lim, C. Tian, J.H. Chung, Y.H. Choung. Isolation and identification of mesenchymal stem cells from human mastoid bone marrow Tissue Engineering and Regeneration Medicine (accepted). 25. K.T. Lim, J.M. Choi, W.C. Lim, J. Kim, H.Y. Cho, J.H. Preparation and characterization of natural material extracted from germinated brown rice. Journal of Biosystems Engineering, 2014:39:235-243. 26. K.T. Lim, J.W. Kim, J. Kim, J.H. Development and evaluation of natural hydroxyapatite ceramics produced by the heat treatment of pig bones. Journal of Biosystems Engineering, 2014:39:227-234. 27. K.T. Lim, J. Kim, J.H. Chung. Development of long-term storage technology for chinese cabbage - Jangho Kim - CV 5/11 update 2015-05-27

physiological characteristics of postharvest freshness in a cooler with a monitoring and control interface. Journal of Biosystems Engineering, 2014:39:194-204. 28. K.T. Lim, J. Kim, J.H. Chung. Development of a vertical embryo-retaining polished rice producing system with abrasive and friction rollers. Food Engineering Progress, 2014:18:242-247. 29. K.T. Lim, S.J Back, S.W. Kim J. Kim, J.W. Kim, H. Seonwoo, P.H. Choung, J.H. Chung. Development of fast- hardening calcium phosphate cements composed of horse-bone derived nanoscale bioceramics and chitosan. Tissue Engineering and Regeneration Medicine (accepted) 30. K.J. Jang, W.J. Cho, H. Seonwoo, J. Kim, J.H. Choung. Development and characterization of horse bone- derived natural calcium phosphate powders. Journal of Biosystems Engineering, (accepted) 31. Y.J. Kim, J. Kim, C. Tian, H.J. Lim, Y.S. Kim, J.H. Chung, Y.H. Choung. Prevention of cisplatin-induced ototoxicity by the inhibition of gap junctional intercellular communication in the auditory cells. Cellular and Molecular Life Sciences (accepted) 32. G. Pankaj, S. Pandey, B. Kang, K.T. Lim, J. Kim, M.H. Cho, T.E. Park, Y.J. Choi, P.H. Choung, C.S. Cho, J.H. Chung. Highly efficient gene transfection by hyperosmotic polymannitol based gene tranporter through regulation of caveolae and COX-2 induced endocytosis. Journal of Materials Chemistry B (accepted) 33. J.D. Suh, K.T. Lim, H. Jin, J. Kim, P.H. Choung, J.H. Chung. Effects of co-culture of dental pulp stem cells and periodontal ligament stem cells on assembled dual disc scaffolds. Tissue Engineering and Regeneration Medicine, 2014:11(1):47-58 34. K.T. Lim, J. Hexiu, J. Kim, P. Garg, S. Pandey, H. Choi, H. Seonwoo, W.J. Cho, P.H. Choung, J.H. Chung. Enhanced osteogenesis using human growth hormone derived from human bone-derived mesenchymal stem cells stimulated by magnetic fields BioMed Research International, 2014. 35. K.T. Lim, J. Hexiu, J. Kim, H. Seonwoo, P.H. Choung, J.H. Chung. Synergistic effects of orbital shear stress on in vitro growth and osteogenic differentiation of human alveolar bone-derived mesenchymal stem cells for tissue engineering applications BioMed Research International, 2014. 36. K.T. Lim, J. Kim, H. Seonwoo, S.H. Park, P.H. Choung, J.H. Chung. In vitro effects of low-intensity pulsed ultrasound stimulation on the osteogenic differentiation of human alveolar bone-derived mesenchymal stem cells for tooth tissue engineering. BioMed Research International, 2014. 37. J. Park, S. Park, S. Ryu, S.H. Bhang, J. Kim, J.K. Yoon, Y.H. Park, S.P. Cho, S. Lee, B.H. Hong, B.S. Kim. Graphene-regulated cardiomyogenic differentiation process of mesenchymal stem cells by enhancing the expression of extracellular matrix proteins and cell signaling molecules. Advanced Healthcare Materials, 2014:3:176-181. 38. H. Seonwoo, S.W. Kim, J. Kim, T. Chunjie, K.T. Lim, Y. Kim, Y.H. Choung, J.H. Chung. Regeneration of chronic tympanic membrane perforation using an EGF-releasing chitosan patch. Tissue Engineering Part A, 2013:19:2097-2107. 39. K.T. Lim, J Hexiu, J. Kim, H. Seonwoo, W.J. Cho, P.H. Choung, J.H. Chung. Effects of electromagnetic fields on osteogenesis of human alveolar bone-derived mesenchymal stem cells. BioMed Research International, 2013. 40. K.T. Lim, S.H. Park, J. Kim, H. Seonwoo, P.H. Choung, J.H. Chung. Cell image processing methods for automatic cell pattern recognition and morphological analysis of mesenchymal stem cells -an algorithm for cell classification and adaptive brightness correction-. Journal of Biosystems Engineering, 2013:38:55-63. Jangho Kim - CV 6/11 update 2015-05-27

41. S. Pandey, P. Garg, K.T. Lim, J. Kim, Y.H. Choung, Y.J. Choi, PH Choung, C.S. Cho, J.H. Chung. The efficiency of membrane transport of vitamin B6 coupled to poly(ester amine) gene transporter and transfection in cancer cells. Biomaterials, 2013:34:3716-3728. 42. H.M. Son, H. Swonwoo, J. Kim, K.T. Lim, J.H. Chung. Development of a system observing worker's physiological responses and 3-dimensional biomechanical loads in the task of twisting while lifting. Journal of Biosystems Engineering, 2013:38:163-170. 43. K.T. Lim, J. Kim, H. Seonwoo, J.W. Chang, P.H. Choung, J.H. Chung. Enhanced osteogenesis of human alveolar bone-derived mesnchymal stem cell for tooth tissue engineering using fluid shear stress in a rocking culture method. Tissue Engineering Part C: Methods, 2012:19:128-145. 44. K.T. Lim, T.S. Park, J. Kim, P.H. Choung, J.H. Chung. Noninvasive measurement of glucose and lactate metabolism of human alveolar bone-derived mesenchymal stem cells using near-infrared spectroscopy. Tissue Engineering and Regeneration Medicine, 2011:8(6):503-511. 45. K.T. Lim, J.D Suh, J. Kim, P.H. Choung, J.H. Chung. Calcium phosphate bioceramics fabricated from extracted human teeth for tooth tissue engineering. Journal of Biomedical Materials Research Part B, 2011:99B(2):399-411. 46. S.J. Choi, S.W. Kim, J. Kim, J.B. Lee, O.S. Choo, J.H. Chung, Y.H. Choung. Efficient treatment of chronic tympanic membrane perforations in animal models by chitosan patch scaffolds. Tissue Engineering and Regeneration Medicine, 2011:8(2):141-150. 47. A.T. Togloom, K.T. Lim, J. Kim, H. Seonwoo, P. Garg, P.H. Choung, C.S. Cho, Y.H. Choung, J.H. Chung. Molecular responses in osteogenic differentiation of mesenchymal stem cells induced by physical stimulation. Tissue Engineering and Regeneration Medicine, 2011:8(3):271-281. 48. H. Seonwoo, K.T. Lim, J. Kim, H.M. Son, J.H. Choung. Measurement of worker’s physiological and biomechanical responses during the cherry tomato harvesting work in a greenhouse. Journal of Biosystems Engineering, 2011:36(3):223-230. 49. K.T. Lim, J. Kim, J.H. Chung. Development of an automatic brown rice germinating system of air-phase type with intermittent water spraying and anion radiation. Journal of Biosystems Engineering, 2011:36(3):187-194. 50. H.S. Choi, K.T. Lim, J. Kim, J.Y. Park, S.H. Jeon, H.W. Choung, C.S. Cho, Y.H. Choung, P.H. Choung, Evaluation of novel degradable poly-bioceramc scaffolds of PDLLA/toothapatite/TCP using human dental pulp stem cells for tooth bioengineering. Tissue Engineering and Regeneration Medicine, 2010:7(5):481-488. 51. K.T. Lim, A.L Im, C.S. Cho, J. Kim, Y.H. Choung, P.H. Choung, H.W. Choung, S.H. Jeon. Novel composite scaffolds for tooth regeneration using human dental pulp stem cells. Tissue Engineering and Regeneration Medicine, 2010:7(5):473-480. 52. H.M. Son, H. Seonwoo, K.T. Lim, J. Kim, J.H. Choung. Analysis of physiological bio-information, human physical activities and load of lumbar spine during the repeated lifting work. Journal of Biosystems Engineering, 2010:35(5):357-365. 53. K.T. Lim, J. Kim, H. Seonwoo, J.H. Hong, J.H. Chung. Effects of electric current stimuli and high-voltage electric field treatments on brown rice germination. Journal of Biosystems Engineering, 2010:35(2):100-107. 54. K.T. Lim, H. Seonwoo, H.M. Son, S.J. Baik, J. Kim, S.M. Hong, C.S. Cho, Y.H. Choung, S.H. Jeon, J.Y. Park, P.H. Choung, J.H. Chung. Development of a perfusion flow bioreactor system for culturing human alveolar bone Jangho Kim - CV 7/11 update 2015-05-27

marrow stem cells. Tissue Engineering and Regeneration Medicine, 2009:6(14):1410-1419. 55. K.T. Lim, J. Kim, H. Seonwoo, H.M. Son, S.J. Baik, J.Y. Park, S.H. Jeon, Y.H. Choung, C.S. Cho, P.H. Choung, J.H. Chung. In vitro effects of electromagnetic field stimulation on cells in tissue engineering. Tissue Engineering and Regeneration Medicine, 2009:6(4-11):675-684. 56. K.T. Lim, C.S. Cho, Y.H. Choung, J. Kim, H.M. Son, H. Seonwoo, S.H. Jeon, J.Y. Park, P.H. Choung, J.H. Chung. Influence of static magnetic field stimulation on cells for tissue engineering. Tissue Engineering and Regeneration Medicine, 2009:6(1-3): 250-258. 57. K.T. Li, C.S. Cho, Y.H. Choung, J. Kim, H.M. Son, H. Seonwoo, S.H. Jeon, J.Y. Park, P.H. Choung, J.H. Chung. Novel perfusion culture bioreactor systems for tissue engineering. Tissue Engineering and Regeneration Medicine, 2009:6(1-3): 199-206. 58. E.T. Lee, K.T. Lee, J. Kim, A.L. Lee, H.M. Son, C.S. Cho, P.H. Choung, J.H. Chung. Effect of low intensity ultrasound stimulation on the proliferation of alveolar bone marrow stem cell. Tissue Engineering and Regeneration Medicine, 2008:5(4-6):572-580. 59. J.H. Choung, J. Kim, Y.H. Choung, A.L. Im, K.T. Lim, J.H. Hong, P.H. Choung. Biomechanical properties and cytotoxicity of chitosan patch scaffold for artificial eardrum. Journal of Biosystems Engineering, 2007:32(1):57-62. 60. K.T. Lim, J.H. Chung, J.H. Hong, J. Kim, E.T. Lee, A.L. Im. Effect of air-phase germination with anion radiation and water-spraying on germination ratio, sprout growth, and GABA contents of germinated brown rice. Agriculture & Biosystems Engineering, 2006:7(1):42-47. [In Progress] 61. Y.J. Kim, J. Kim, C. Tian, Y.S. Kim, H.J. Kim, J. H. Chung, Y.H. Choung. The role of junction-independent connexin 43 on cisplatin-induced ototoxicity in auditory cells (under review). 62. Y.J. Kim, J. Kim, C. Tian, Y.S. Kim, H.J. Kim, Y.H. Choung. Inhibition of autophagolysosomal fusion contributes to aminoglycoside-induced delayed ototoxicity (under review). 63. K.T. Lim, S.J Back, S.W. Kim J. Kim, J.W. Kim, H. Seonwoo, P.H. Choung, J.H. Chung. Rapid setting behaviors and histological evaluation of 3D calcium phosphate bone cement using nanoscale bioceramics derived from pig bones and chitosan (under review). 64. K.T. Lim, S.J. Baik, J. Kim, J.H. Chung. Mechanical and biological behaviors of reinforced bone cement composed of nanoscale materials and chitosan originated from agricultural by-products (submitted). 65. K.T. Lim, J.W. Kim, J. Kim, P.H. Choung, J.H. Chung. Development of a novel water-jacketed mobile culture system with automatic process control for stem cells. Biosystems Engineering (under revision)

C. Invited Talks and Seminars: 1. Multiscale engineered biomimetic approaches for regulation of cell function and tissue regeneration. Chonnam National University School of Dentistry, April 28, 2015 2. Multiscale engineered biomimetic approaches for regulation of cell function and tissue regeneration. , April 15, 2015 3. Nanoengineered biomimetic approaches for regulation of cell function and tissue regeneration. Konyang University School of Medicine, November 14, 2014 4. Nanoengineered biomimetic approaches for regulation of cell function and tissue regeneration. TERM STEM 2014, Porto, Portugal, October 23-24, 2014. (Keynote Speaker) Jangho Kim - CV 8/11 update 2015-05-27

5. Nanoengineered biomimetic approaches for regulation of cell function and tissue regeneration. (SKKU), October 13, 2014 6. Nanoengineered biomimetic approaches for regulation of cell function and tissue regeneration. UNIST, August 29, 2014 7. Mechanobiological regulation of structure and function of stem cells by biologically-inspired nanotopography. Tissue Engineering & Regenerative Medicine International Society Asia-Pacific (TERMIS-AP) Conference, Shanghai, P.R. China, October 23-26, 2013. (Young Plenary Speaker) 8. Unique and valuable experience as a YIC member. Tissue Engineering & Regenerative Medicine International Society Asia-Pacific (TERMIS-AP) Conference, Shanghai, P.R. China, October 25, 2013. 9. Micro, and nanoengineering systems for controlling cellular structure and function. Shanghai Ocean University, October 23, 2013.

D. Conference Proceedings and Abstracts (selected): 1. J. Kim, J.H. Chung, Nanoengineered biomimetic approaches for regulation of cell function and tissue regeneration. TERM STEM 2014, Porto, Portugal, October 23-24, 2014. (Keynote Speaker) 2. J. Kim, Mechanobiological regulation of structure and function of stem cells by biologically-inspired nanotopography. Tissue Engineering & Regenerative Medicine International Society Asia-Pacific (TERMIS-AP) Conference, Shanghai, P.R. China, October 23-26, 2013. (Young Plenary Speaker) 3. J. Kim, H.N. Kim, K.T Lim, Y. Kim, H. Seonwoo, Y.H. Choung, K.Y. Suh, J.H. Chung, Mechanobiological regulation of structure and function of stem cells by biologically-inspired nanonegineering systems. 2013 Annual Meeting of Korean Society for Stem Cell Research, Korea. August 29-30, 2013. (Best Presentation Award) 4. J. Kim, H.N. Kim, K.T Lim, Y. Kim, H. Seonwoo, Y.H. Choung, K.Y. Suh, J.H. Chung, Designing nanotopography-based synthetic extracellular matrix for controlling structure and function of stem cells. 2013 Annual Conference of Korean Tissue Engineering and Regenerative Medicine Society, Korea. May 31- June 01, 2013. (Outstanding Research Award) 5. J. Kim, H.N. Kim, K.T Lim, K.Y. Suh, J.H. Chung, Biologically inspired nanotopography as a strategy for engineering structure and function of stem cells. Proceedings of the KSAM 2013 Spring Conference. Korea. . May 2013. (Best Oral Presentation Award) 6. J. Kim, H.N. Kim, K.T Lim, Y. Kim, H. Seonwoo, D.H. Kim, Y.H. Choung, K.Y. Suh, J.H. Chung, Nanotopography-guided stem cell engineering: regulating structure and function of human mesenchymal stem cells on precisely defined nanogroove patterns. The 20th annual meeting of the Korean Society for Biomaterials, Seoul, Korea. November 15-16, 2012. (Young Scientist Award) 7. J. Kim, K.T Lim, H.N. Kim, Y. Kim, H. Seonwoo, D.H. Kim, Y.H. Choung, K.Y. Suh, J.H. Chung, Controlling structure and function of human mesenchymal stem cells by bioinspired nanotopography. Combined Congress of Otorhinolarynology-Head and Neck Sugery, Seoul, Korea. November 15-16, 2012. 8. J. Kim, K.S. Choi, K.T. Lim, Y. Kim, H. Seonwoo, D.H. Kim, P.H. Choung, C.S. Cho, S.Y. Kim, Y.H. Choung, J.H. Chung. Modulating behaviors of human adipose-derived stem cells using graphene-based artificial extracellular matrices. 2012 3th TERMIS World Congress, Vienna, Auatria. September, 4-7, 2012. 9. J. Kim, K.T. Lim, H. Seonwoo, S.H. Park, J.H. Chung. Charged nanomatrices as efficient platforms for modulating cell adhesion and shape. 2012 3th TERMIS World Congress, Vienna, Auatria. September, 4-7, Jangho Kim - CV 9/11 update 2015-05-27

2012. 10. J. Kim, H.N. Kim, Y. Kim, K.T. Lim, H. Seonwoo, D.H. Kim, P.H. Choung, Y.H. Choung, K.Y. Suh, J.H. Chung, Bioinspired nanotopography-induced human mesenchymal stem cell behaviors on precisely defined nanogroove platforms, The 8th Annual Meeting of Korean Society for Stem Cell Research, Incheon, Korea. August 30-31, 2012. 11. J. Kim, K.S. Choi, K.T. Lim, Y. Kim, H. Seonwoo, D.H. Kim, P.H. Choung, C.S. Cho, S.Y. Kim, Y.H. Choung, J.H. Chung. Regulating stem cell behaviors using graphene-based platfroms. 2012 Annual Conference of Korean Tissue Engineering and Regenerative Medicine Society, Korea. (Outstanding Research Award) 12. J. Kim, K.T. Lim, H. Seonwoo, J.H. Chung. Development of graphene-based nano platforms for adherent cell culture. Proceedings of the KSAM 2012 Winter Conference. Korea. (Best Poster Presentation Award) 13. J. Kim, K.T. Lim, H. Seonwoo, S.H. Park, J.H. Chung. Charged nanomatrices as efficient platforms for modulating cell adhesion and shape. Proceedings of the 6th International Symposium on Machinery and Mechatronics for Agriculture and Biosystems Engineering (ISMAB) June 18-20, 2012, Jeonju, Korea. 14. J. Kim, K.T. Lim, H. Seonwoo, J.H. Choung. Graphene as an enabling tool for biological engineering applications: adherent cell culture on graphene-based nano platforms. Proceedings of the 6th International Symposium on Machinery and Mechatronics for Agriculture and Biosystems Engineering (ISMAB) June 18-20, 2012, Jeonju, Korea. 15. J. Kim, D.H. Kim, K.T. Lim, H. Seonwoo, S.H. Park, Y.R. Kim, Y.H. Choung, P.H. Choung, J.H. Choung. Charged nanomatrices as efficient platforms for modulating cell adhesion and shape. 5th IEEE International Conference on Nano/Molecular Medicine and Engineering, Jeju, Korea, November 9-12, 2011. 16. J. Kim, S.W. Kim, K.T. Lim, S.J. Choi, H. Seonwoo, P.H. Choung, Y.H. Choung, J.H. Chung. Chitosan platforms as an enabling tool for tympanic membrane regeneration. The 3rd Asian Biomaterials Congress, Korea. 2011. 17. J. Kim, S.J. Choi, K.T. Lim, P.H. Choung, H.M. Som, H. Seonwoo, S.J. Baik, Y.H. Choung, J.H. Chung. Tympanic membrane regeneration using a chitosan patch scaffold. Tissue Engineering & Regenerative Medicine International Society World Congress, Korea 2009. 6(12):946. 18. J. Kim, K.T. Lim, P.H. Choung, E.T. Lee, H.M. Son, Y.H. Choung, C.S. Cho, J.H. Chung. Development of nano- matrix composed of poly(ε-caprolactone) and polyethylenimine using electrospinning system for tissue engineering. 2009 ASABE Annual International Meeting. USA. 19. J. Kim, I.Y. Kim, P.H. Choung, K.T. Lim, Y.H. Choung, E.T. Lee, A.L, Im, C.S. Cho, J.H. Chung. Structural characteristics, mechanical properties and cytotoxicity of PEI/PCL electrospun nanofiber for tissue engineering applications. Proceedings of the KSAM 2008 Winter Conference. Korea. 2008, 13(1): 388-393. (Best Poster Presentation Award) 20. J. Kim, J.H. Bae, J.H. Chung, Y.H. Choung, K.T. Lim, S.J. Choi, E.T. Lee, A.L. Im. Development of artificial eardrum patch to repair tympanic membrane perforations. 2008 ASABE Annual International Meeting. USA, 2008. 21. J. Kim, I.Y. Kim, K.T. Lim, P.H. Choung, C.S. Cho, J.H. Chusng. Development of nano-matrix composed of polyethylenimine and poly(ε-caprolactone) for gene delivery and tissue engineering. 2008 Annual Conference of Korean Tissue Engineering and Regenerative Medicine Society. Korea, 2008. 22. J. Kim, J.H. Choung, Y.H. Choung, K.T. Lim, E.T. Lee, A.L. Im, J.H. Hong, C.S. Cho, J.H. Chung. Fabrication of chitosan-coated biodegradable polymer nanofiber scaffold using an electrospinning system for tissue Jangho Kim - CV 10/11 update 2015-05-27

engineering applications. Proceedings of the KSAM 2007 Summer Conference. Korea, 2007, 12(2): 309- 313. (Best Poster Presentation Award) 23. J.H. Chung, J. Kim, Y.H. Choung, A.L. Im, K.T. Lim, J.H. Hong. Biomechanical properties and cytotoxicity of chitosan patch scaffold for artificial eardrum. Proceedings of the 3rd International Symposium on Machinery and Mechatronics for Agriculture and Biosystems Engineering (ISMAB) November 23-25 2006, Seoul, Korea.

Jangho Kim - CV 11/11 update 2015-05-27