DFFA Monoclonal Antibody (M05), Degradation of the Chromosomal DNA Into Nucleosomal Clone 3A11 Units

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DFFA monoclonal antibody (M05), degradation of the chromosomal DNA into nucleosomal clone 3A11 units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. Catalog Number: H00001676-M05 DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated Regulatory Status: For research use only (RUO) when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active Product Description: Mouse monoclonal antibody component of DFF. DFFB has been found to trigger both raised against a partial recombinant DFFA. DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants Clone Name: 3A11 encoding distinct isoforms have been found for this gene. [provided by RefSeq] Immunogen: DFFA (NP_004392.1, 231 a.a. ~ 331 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence: TSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPK ALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLR SISASKASPPGDLQNPKRARQDPT Host: Mouse Reactivity: Human Applications: ELISA, PLA-Ce, S-ELISA, WB-Re, WB-Tr (See our web site product page for detailed applications information) Protocols: See our web site at http://www.abnova.com/support/protocols.asp or product page for detailed protocols Isotype: IgG2a Kappa Storage Buffer: In 1x PBS, pH 7.4 Storage Instruction: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 1676 Gene Symbol: DFFA Gene Alias: DFF-45, DFF1, ICAD Gene Summary: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and Page 1/1 Powered by TCPDF (www.tcpdf.org).

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DFFB (NM 004402) Human Tagged ORF Clone Product Data
OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC208266 DFFB (NM_004402) Human Tagged ORF Clone Product data: Product Type: Expression Plasmids Product Name: DFFB (NM_004402) Human Tagged ORF Clone Tag: Myc-DDK Symbol: DFFB Synonyms: CAD; CPAN; DFF-40; DFF2; DFF40 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin ORF Nucleotide >RC208266 ORF sequence Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCAGAAGCCCAAGAGCGTGAAGCTGCGGGCCCTGCGCAGCCCGAGGAAGTTCGGCGTGGCTGGCC GGAGCTGCCAGGAGGTGCTGCGCAAGGGCTGTCTCCGCTTCCAGCTCCCTGAGCGCGGTTCCCGGCTGTG CCTGTACGAGGATGGCACGGAGCTGACGGAAGATTACTTCCCCAGTGTTCCCGACAACGCCGAGCTGGTG CTGCTCACCTTGGGCCAGGCCTGGCAGGGCTATGTGAGCGACATCAGGCGCTTCCTCAGTGCATTTCACG AGCCACAGGTGGGGCTCATCCAGGCCGCCCAGCAGCTGCTGTGTGATGAGCAGGCCCCACAGAGGCAGAG GCTGCTGGCTGACCTCCTGCACAACGTCAGCCAGAACATCGCGGCCGAGACCCGGGCTGAGGACCCGCCG TGGTTTGAAGGCTTGGAGTCCCGATTTCAGAGCAAGTCTGGCTATCTGAGATACAGCTGTGAGAGCCGGA TCCGGAGTTACCTGAGGGAGGTGAGCTCCTACCCCTCCACAGTGGGTGCGGAGGCTCAGGAGGAATTCCT GCGGGTCCTCGGCTCCATGTGCCAGAGGCTCCGGTCCATGCAGTACAATGGCAGCTACTTCGACAGAGGA GCCAAGGGCGGCAGCCGCCTCTGCACACCGGAAGGCTGGTTCTCCTGCCAGGGTCCCTTTGACATGGACA GCTGCTTATCAAGACACTCCATCAACCCCTACAGTAACAGGGAGAGCAGGATCCTCTTCAGCACCTGGAA CCTGGATCACATAATAGAAAAGAAACGCACCATCATTCCTACACTGGTGGAAGCAATTAAGGAACAAGAT GGAAGAGAAGTGGACTGGGAGTATTTTTATGGCCTGCTTTTTACCTCAGAGAACCTAAAACTAGTGCACA
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Proteomic Analysis of Ubiquitin Ligase KEAP1 Reveals Associated Proteins That Inhibit NRF2 Ubiquitination
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Produktinformation
Produktinformation Diagnostik & molekulare Diagnostik Laborgeräte & Service Zellkultur & Verbrauchsmaterial Forschungsprodukte & Biochemikalien Weitere Information auf den folgenden Seiten! See the following pages for more information! Lieferung & Zahlungsart Lieferung: frei Haus Bestellung auf Rechnung SZABO-SCANDIC Lieferung: € 10,- HandelsgmbH & Co KG Erstbestellung Vorauskassa Quellenstraße 110, A-1100 Wien T. +43(0)1 489 3961-0 Zuschläge F. +43(0)1 489 3961-7 [email protected] • Mindermengenzuschlag www.szabo-scandic.com • Trockeneiszuschlag • Gefahrgutzuschlag linkedin.com/company/szaboscandic • Expressversand facebook.com/szaboscandic DFFA Recombinant Protein (OPCD02721) Data Sheet Product Number OPCD02721 Product Page http://www.avivasysbio.com/dffa-recombinant-protein-opcd02721.html Product Name DFFA Recombinant Protein (OPCD02721) Size 10 ug Gene Symbol DFFA Alias Symbols A330085O09Rik, DFF35, Dff45, DFF-45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Icad, ICAD, ICAD-L, IC AD-S, Inhibitor of CAD Molecular Weight 31 kDa Product Format Lyophilized Tag N-terminal His Tag Conjugation Unconjugated NCBI Gene Id 13347 Host E.coli Purity > 95% Source Prokaryotic Expressed Recombinant Official Gene Full Name DNA fragmentation factor, alpha subunit Description of Target Inhibitor of the caspase-activated DNase (DFF40). Reconstitution and Storage Reconstitute in PBS or others. Store at 2-8C for one month. Aliquot and store at -80C for 12 months. Avoid repeated freeze/thaw cycles. Additional Information Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method) Additional Information Residues: Lys19 - Ser262 Lead Time Domestic: within 1-2 weeks delivery International: 1-3 weeks Formulation Lyophilized in PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Cddpre6 8 5..5
Cell Death and Differentiation (1999) 6, 745 ± 752 ã 1999 Stockton Press All rights reserved 13509047/99 $15.00 http://www.stockton-press.co.uk/cdd Structure and promoter analysis of murine CAD and ICAD genes Kohki Kawane1,2, Hidehiro Fukuyama1,2, Masashi Adachi1,2, condensation, and formation of apoptotic bodies,2 and Hideki Sakahira1,2, Neal G. Copeland3, Debra J. Gilbert3, biochemically by degradation of chromosomal DNA into 3 Nancy A. Jenkin3 and Shigekazu Nagata*,1,2 nucleosomal units. Most of the apoptotic stimuli activate caspases, which are cysteine proteases that cleave various 1 Department of Genetics, Osaka University Medical School, Osaka, Japan substrates such as poly(ADP) ribose polymerase, lamin, and 2 Core Research for Evolutional Science and Technology, Japan Science and fodrin to promote the apoptotic process.4±7 We and others Technology Corporation, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan recently showed that a DNase (CAD, caspase-activated 3 Mammalian Genetics Laboratory, ABL-Basic Research Program, National DNase; also called CPAN, caspase activated nuclease, as Cancer Institute-Frederick Cancer Research and Development Center, well as DFF-40, DNA fragmentation factor-40) is activated Frederick, Maryland 21702, USA during apoptosis, and cleaves the chromosomal DNA.8±12 In * corresponding author: Shigekazu Nagata, Ph.D., Department of Genetics, Osaka University Medical School B-3, 2-2 Yamada-oka, Suita, Osaka 565- the growing, non-apoptotic cells, CAD is complexed with its 0871, Japan. inhibitor (ICAD, inhibitor of CAD; also called DFF-45) and tel: 81-6-6879-3310; fax: 81-6-6879-3319; predominantly localized in the cytoplasm.
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DFFA Recombinant Protein Description Product Info
9853 Pacific Heights Blvd. Suite D. San Diego, CA 92121, USA Tel: 858-263-4982 Email: [email protected] 32-3671: DFFA Recombinant Protein DNA fragmentation factor subunit alpha,DNA fragmentation factor 45 kDa subunit,DFF-45,Inhibitor of Alternative Name : CAD,ICAD,DFFA,DFF1,DFF45. Description Source : Escherichia Coli. DFFA Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 351 amino acids (1- 331 a.a.) and having a molecular mass of 38.7kDa.The DFFA is purified by proprietary chromatographic techniques. DFF is a heterodimeric protein of 40kDa (DFFB) and 45kDa (DFFA) subunits. DFFA (DNA fragmentation factor subunit alpha) is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF is activated once DFFA is cleaved by caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.DFFA at chromosome 1 shows rare allelic variants in neuroblastoma tumors. Product Info Amount : 25 µg Purification : Greater than 90.0% as determined by SDS-PAGE. Content : The DFFA solution contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT. DFFA although stable 4°C for 4 weeks, should be stored desiccated below -18°C.
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