think ! think G-Biosciences! From Extraction To Protein Detection & Estimation

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• Protein Estimation • Western Blotting • Extraction & Lysis • Mass Spectrometry • Fractionation & Enrichment • Purification & Chromatography • Sample Preparation • Labeling & Conjugation • Protein Electrophoresis • Antibody Production & Purification Lysis System for Active & Recombinant Proteins 0.8 0.020 ™ Alkaline Phosphatase PE LB (Protein Extraction & Lysis Buffer) 0.7 Carbonic Anhydrase Affordable Extraction of Active, Soluble Proteins 0.6 0.015 0.5

0.4 0.010

0.3

• Based on Proven & Reliable Enzyme Lysis Methods 0.2 0.005

0.1

• Simple, Fast & Ready-to-Use Activity (units/ml) Anhydrase Carbonic Alkaline Phosphatase Activity (units/ml) Phosphatase Alkaline 0 0 • Uses Innovative LongLife™ Enzyme Solutions E.coli Human cells Yeast Mouse Pancreas • Ideal for Active Enzymes (Top figure) • Ideal for Recombinant Proteins (Bottom figure) • Available for Bacteria, Mammalian Cell, Tissue , Yeast & Insect Samples

50%+ Savings vs. Competitors Protease Inhibitor Cocktails ™ 100 ProteaseArrest Protease Inhibitor Solution 90 Instant Inhibition of >95% Protease Activity 80 70

60 5-fold more • 100X Concentrated, Ready-to-Use Solution 50 inhibition • Excellent General Protease Inhibitor Cocktail 40

• Outperforms Commercial Tablet Cocktails by >5X (see figure) 30

• No Preparation Solution Format Allows For Instant Inhibition! 20 Protease inhibition (%) inhibition Protease

• For Specialized Inhibition; Species Specific Formats Available: 10 ProteaseARREST Bacterial, Yeast, Fungal, Plant & Mammalian 0 ProteaseARREST™ Tablet Cocktail Protein Estimation AssayS CB-X™ Protein * Bradford, M.M. (1976) Anal. Biochem. 72:248-54 A Bradford* Assay That Does More Than Your Average Protein Assays

• 1 Assay, 2 Key Approaches • Standard Assay: Single Solution for Most Assays • Clean Up Assay: A Unique Clean Up Reagent for POOR: Non linear response ™ Problematic Situations (see figure) • Ideal for All Laboratory Protein Estimations • Overcomes Majority of Common Assay Interfering Agents • Improved Accuracy & Reproducibility PERFECT: Linear response with CB-X DIALYSIS WITH NO SAMPLE LOSS Tube-O-DIALYZER™ Dialysis System Unique Tube Format for Simple Dialysis

• 100% Sample Recovery • Trouble Free Sample & Addition, Simple Pipetting • Centrifuge to Recover, Even Protein Precipitates • Save >30% Compared to Commercial Dialysis Cassettes • Wide MWCO Range: 1K, 4K, 8K, 15K & 50K • For 20-250µl & 0.2-2.5ml Sample Volumes

Protein Electrophoresis: GEL Stain & Ladder LabSafe GEL Blue™ Tri-color ™ Ultra Sensitive & Rapid Coomassie Stain ™ mark PAGE mark PAGE • Sensitive: Detects as little as 4ng BSA • Rapid: Protein visible in <10 minutes • Versatile: Stains native, denaturing, IEF and 2D gels • Simple: Ready-to-use & no destaining PAGEmark™ Protein Ladder

• 8 prestained protein markers (7-210kDa) • All blue or tri-colored • Available in single use aliquots

Western Blot Detection in <90 Minutes Swift™ Western Diluent A Single Reagent for Fast Track Western Blots

• No Optimization, Use Exisiting Antibody Conditions • Not Limited to Mouse & Rabbit Antibodies • Use Your Secondary Antibody of Choice • Single Reagent Offers Affordability Fewer Steps, Shorter Time! Recombinant Complete 6X His or GST Tagged Protein Purification Rapid Purification of Soluble Recombinant Proteins from Bacteria

• Supplied with all reagents, includes lysis buffers and lytic enzymes • Gravity or spin-format • Nickel or Cobalt Chelating Resin for 6X His • High capacity: >50mg/ml. Higher than leading competitor! Nickel Resin • High binding Glutathione Resin for GST • Save 30-50% on bulk quantities compared to leading competitor!

Cobalt Resin Reagent Size G-Biosciences Cat. VWR Cat. No.

Bacterial PE LB™ with PE LB™ Lysozyme 100 Preps 786-176 82021-546

Bacterial PE LB™ 500ml 786-177 82022-592

Yeast PE LB™ with Zymolyase® 100 Preps 786-178 82023-266

Yeast PE LB™ 500ml 786-179 82022-594

Mammalian Cell PE LB™ 500ml 786-180 82022-596

Tissue PE LB™ 500ml 786-181 82022-598

Insect PE LB™ 250ml 786-411 95057-584 Glutathione Resin Figure: A 6X His tagged protein (Top panels) and a GST tagged ProteaseArrest™ [100X] 2ml 786-108 82022-478 protein (Bottom panel) were purified from 50ml bacterial culture using the appropriate HOOK™ Spin Purification kit. The OneQuant™ ProteaseArrest™ [100X] 24 x 100µl 786-329 95043-418 Nickel resin purified a large amount of 6X His tagged protein, compared to the cobalt resin, however the cobalt purified Bacterial ProteaseArrest™ [100X] 1ml 786-330 78000-034 protein was purer. Lanes: 1. PAGEmark™ protein ladder, 2. Cleared Mammalian ProteaseArrest™ [100X] 1ml 786-331 78000-036 lysate, 3. Flow through, 4-6. Sequential washes, 7-10. Sequential elutions. Plant ProteaseArrest™ [100X] 1ml 786-332 78000-038

Yeast/ Fungal ProteaseArrest™ [100X] 1ml 786-333 78000-040

Recom ProteaseArrest™ [100X] 1ml 786-376 95057-582

CB-X™ Protein Assay with Standard 500 Assays 786-12X 82021-292

Tube-O-DIALYZER™, Micro 1K MWCO 20 786-610 95057-588

Tube-O-DIALYZER™, Micro 4K MWCO 20 786-611 95057-590

Tube-O-DIALYZER™, Micro 8K MWCO 20 786-612 95057-592

Tube-O-DIALYZER™, Micro 15K MWCO 20 786-613 95057-594

Tube-O-DIALYZER™, Micro 50K MWCO 20 786-614 95057-596

Tube-O-DIALYZER™, Medi, 1K MWCO 20 786-615 95057-598

Tube-O-DIALYZER™, Medi, 4K MWCO 20 786-616 95057-600

Tube-O-DIALYZER™, Medi, 8K MWCO 20 786-617 95057-602 Tube-O-DIALYZER™, Medi, 15K MWCO 20 786-618 95057-604 ® Tube-O-DIALYZER™, Medi, 50K MWCO 20 786-619 95057-606

LabSafe GEL Blue™ 1 Liter 786-35 82021-412

LabSafe GEL Blue™ 1 Gallon 786-35G 82021-414

PAGEmark™ Tri-color 100 Loads 786-412 89167-754

PAGEmark™ Protein Marker 100 Loads 786-415 89167-756

OneQuant™ PAGEmark™ 40 Loads 786-416 89167-758

OneQuant™ PAGEmark™ Tri-color 40 loads 786-417 89167-760

Swift Western Diluent 125ml 786-679 71003-234

HOOK™ GST Protein Spin Purification Kit (Bacteria) 25 786-640 89167-834

HOOK™ GST Protein Purification Kit (Bacteria) 5 786-641 89167-836

HOOK™ 6X His Protein Spin Purification Kit (Bacteria): Nickel Resin 25 786-628 89167-818

HOOK™ 6X His Protein Spin Purification Kit (Bacteria): Cobalt Resin 25 786-629 89167-820

HOOK™ 6X His Protein Purification Kit (Bacteria): Nickel Resin 5 786-630 89167-822 HOOK™ 6X His Protein Purification Kit (Bacteria): Cobalt Resin 5 786-631 89167-824 0112 Lit. No. POD 07074