ProceedIngs OF THE National Academy of Sciences OF THE OF AMERICA

November 1980 Volume 77, Number 11 pp. 6255-6942 Table of Contents

INFORMATION TO CONTRIBUTORS 111-v

AUTHOR INDEX vii

Physical Sciences APPLIED MATHEMATICAL SCIENCES Computers-Present and future: Introduction to the symposium E. R. Piore 6255 Computers-Work in progress Herman H. Goldstine 6256-6259 Computers-Small computers: Past, present, and future Bernard M. Oliver 6260-6261 Computers-The software situation Jacob T. Schwartz 6262-6263 Computers-Non-numerical computation Herbert A. Simon 6264-6268 APPLIED PHYSICAL SCIENCES Dedicated-site, interim storage of high-level nuclear waste as part of the E-an Zen 6269-6271 management system Phase separation in mixtures of ionized iron in a hydrogen plasma B. J. Alder, E. L. Pollock, and J. P. 6272-6274 (asymmetric plasma mixtures/hypernetted chain/molecular dynamics) Hansen ASTRONOMY Statistical invalidation of the Hubble law (galaxies/cosmology/redshift- J. F. Nicoll, D. Johnson, I. E. Segal, 6275-6279 distance relationship) and W. Segal CHEMISTRY Synthesis and characterization of a photosensitive interface for hydrogen Dana C. Bookbinder, James A. Bruce, 6280-6284 generation: Chemically modified p-type semiconducting silicon Raymond N. Dominey, Nathan S. photocathodes (solar energy/photoelectrochemistry/energy conversion) Lewis, and Mark S. Wrighton Chemical potential, Teller's theorem, and the scaling of atomic and molecular Norman H. March and Robert G. Parr 6285-6288 energies (Thomas-Fermi theory/electronegativity) 25Mg NMR in DNA solutions: Dominance of site binding effects (alkaline earth D. Murk Rose, M. L. Bleam, M. T. 6289-6292 ions/ion condensation theory/NMR relaxation) Record, Jr., and R. G. Bryant Analytic construction of Hartree-Fock density matrices (density functional/ Philip W. Payne 6293-6297 Hohenberg-Kohn/molecular orbital/electron density/exchange correlation)

i Contents

GEOPHYSICS A two-reservoir recycling model for mantle-crust evolution (mantle depletion! S. B. Jacobsen and G. J. Wasserburg 6298-6302 crustal growth/trace elements/neodymium isotopes/strontium isotopes) MATHEMATICS Spinor representations of affine Lie algebras (Clifford algebra/Clifford module/ I. B. Frenkel 6303-6306 fundamental representation/quantum field theory/elliptic 0-functions) PHYSICS Why is there no bulk specific heat anomaly at the superconducting transition Christoph E. Methfessel, Gregory R. 6307-6308 temperature of BaPbl-.Bi,,03? (superconductivity/oxide/exciton Stewart, Bernd T. Matthias, and C. mechanism) K. N. Patel

Biological Sciences 6309-6942

ii Proceedings OF THE National Academy of Sciences OF THE UNITED STATES OF AMERICA

November 1980 Volume 77, Number 11 pp. 6255-6942 Table of Contents

INFORMATION TO CONTRIBUTORS ix-xii AUTHOR INDEX xiii-xiv

Physical Sciences 6255-6308

Biological Sciences

BIOCHEMISTRY Fast algorithm for predicting the secondary structure of single-stranded RNA Ruth Nussinov and Ann B. Jacobson 6309-6313 (computer program/polynucleotideiRNA folding) Wavelength regulation in rhodopsin: Effects of dipoles and amino acid side Thomas R. Hays, S. H. Lin, and H. 6314-6318 chains (point charge/perturbation) Eyring Specific labeling of the lac carrier in membrane vesicles of Escherichia G. J. Kaczorowski, G. LeBlanc, and H. 6319-6323 coli by a photoaffinity reagent (active transport/nitrophenyl ether! R. Kaback nucleophilic aromatic photosubstitution/triplet state/scavenging) Permeabilization of animal cells for kinetic studies of intracellular enzymes: In Juan J. Aragon, Juan E. Feliu, Ren6 -6324-6328 situ behavior of the glycolytic enzymes of erythrocytes (macromolecular A. Frenkel, and Alberto Sols environment/protein-protein interactions/metabolic control/enzyme regulation/phosphofructokinase) Autonomously replicating sequences in Saccharomyces cerevisiae Clarence S. M. Chan and Bik-Kwoon 6329-6333 (chromosomal replicating segments/baker's yeast/repetitive sequences/ Tye transformation) Ligand substitution reactions of metallothioneins with EDTA and apo-carbonic Ta-Yuen Li, Alan J. Kraker, C. Frank 6334-6338 anhydrase (cadmium/zinc/metal ion transfer/metalloprotein Shaw III, and David H. Petering reconstitution) Contents

Construction of a flash-activated cyclic electron transport system by using Nigel K. Packham, David M. Tiede, 6339-6343 bacterial reaction centers and the ubiquinone-cytochrome b-ci/c segment of Paul Mueller, and P. Leslie Dutton mitochondria (photochemical reaction center/mitochondria complexI1I/ cytochromes b and c/antimycin A) Properties of the separated catalytic and regulatory units of brain adenylate Stephen Strittmatter and Eva J. Neer 6344-6348 cyclase (guanine nucleotide regulation/detergent solubilization/enzyme reconstitution) Multiple mRNA species for the precursor to an adenovirus-encoded Hakan Persson, Hans Jornvall, and 6349-6353 glycoprotein: Identification and structure of the signal sequence (mRNA Jan Zabielski purification/protein structure analysis/binding to transplantation ) Charge movements during the Na+-Ca2+ exchange in heart sarcolemmal P. Caroni, L. Reinlib, and E. Carafoli 6354-6358 vesicles (permeability to K+/permeability to Clh/chlorprornazine) Glutathione transport by inside-out vesicles from human erythrocytes (cytidine Takahito Kondo, George L. Dale, and 6359-6362 monophosphate/inhibition/kinetics) Ernest Beutler Proteolytic inactivation of luciferases from three species of luminous marine Thomas F. Holzman and Thomas 0. 6363-6367 bacteria, Beneckea harveyi, Photobacterium fischeri, and Photobacterium Baldwin phosphoreum: Evidence of a conserved structural feature (limited proteolysis/active center/bioluminescence) A nondenaturing zwitterionic detergent for membrane : Design and Leonard M. Hjelmeland 6368-6370 synthesis (solubilization) Redox conformation changes in refined tuna cytochrome c (x-ray diffraction/ Tsunehiro Takano and Richard E. 6371-6375 electron transfer mechanism/protein conformation changes/buried water Dickerson molecule/heme motion in protein) The capsid protein of Semliki Forest has clusters of basic amino acids and H. Garoff, A.-M. Frischauf, K. 6376-6380 prolines in its amino-terminal region (nucleotide sequencing/cDNA/ Simons, H. Lehrach, and H. Delius molecular /protein-RNA interactions) In vitro comparison of initiation properties of X wild-type PR and Diane K. Hawley and William R. 6381-6385 x3 mutant promoters (RNA polymerase mechanism/abortive initiation) McClure Sodium-dependent silicate transport in the apochlorotic marine diatom Pinakilal Bhattacharyya and 6386-6390 Nitzschia alba (Na + gradient/Na+,K*-ATPase/membrane vesicles) Benjamin E. Volcani Ion-transport chain of cytochrome oxidase: The two chain-direct coupling Mitchell Fry and David E. Green 6391-6395 principle of energy coupling (cardiolipin/ion-transport cage/fixed ionophore/ferrocytochrome c:oxygen oxidoreductase) Specificity in interaction of benzo[alpyrene with nuclear macromolecules: Michael C. MacLeod, Arend Kootstra, 6396-6400 Implication of derivatives of two dihydrodiols in protein binding (histone/ Betty K. Mansfield, Thomas J. polynuclear aromatic hydrocarbon/bay region theory of chemical Slaga, and James K. Selkirk /reverse diol epoxide) Cytoskeletal constraint of the f3-adrenergic receptor in frog erythrocyte Bruce D. Cherksey, Jose A. 6401-6405 membranes (propranolol//adenylate cyclase/fluorescence/ Zadunaisky, and Randall B. catecholamines) Murphy Synthesis, intracellular distribution, and secretion of multiple forms of Jeremiah J. Morrissey, Robert E. 6406-6410 parathyroid secretory protein-I (parathyroid hormone/membranes/ Shofstall, James W. Hamilton, and precursor peptide/glycoprotein secretory mechanisms) David V. Cohn Role of kidney tissue in the production of 25-hydroxyvitamin D3-26,23-lactone Yoko Tanaka, Joseph K. Wichmann, 6411-6414 and la,25-dihydroxyvitamin D3-26,23-lactone (vitamin D/kidney/25- Herbert E. Paaren, Heinrich K. hydroxyvitamin D/1,25-dihydroxyvitamin D) Schnoes, and Hector F. DeLuca Mitochondrial DNA polymorphism: Evidence that variants detected by Frank J. Castora, Norman Arnheim, 6415-6419 restriction enzymes differ in nucleotide sequence rather than in methylation and Melvin V. Simpson (mtDNA evolution/mtDNA cloning/sequence analysis/rats) Relationship of GIX expression to the glycosylation of murine Marsha Rich Rosner, Jwu-Sheng 6420-6424 virus glycoprotein (loss of antigenicity/determination of oligosaccharide Tung, Nancy Hopkins, and Phillips chain number/endo-13-N-acetylglucosaminidase H) W. Robbins Protein p3 is linked to the DNA of phage 029 through a phosphoester bond Jose M. Hermoso and Margarita Salas 6425-6428 between serine and 5'-dAMP (initiation of replication/Bacillus subtilis/ nucleotidyl-peptide/acid and alkali hydrolysis) In vivo regulation of rat liver 3-hydroxy-3-methylglutaryl-coenzyme A Ronald E. Arebalo, Joel E. Hardgrave, 6429-6433 reductase: Enzyme phosphorylation as an early regulatory response after Billie J. Noland, and Terence J. intragastric administration of mevalonolactone (cholesterol biosynthesis) Scallen Biosynthesis of A,B procollagen (processing/type V collagen/chicken embryo/ Carol A. Kumamoto and John H. 6434-6438 muscle) Fessler Specific photoaffinity labeling induced by energy transfer: Application to Maurice P. Goeldner and Christian G. 6439-6442 irreversible inhibition of acetylcholinesterase (covalent labeling/ Hirth fluorescence quenching/cholinergic probe) ii Contents Aggregation of small oligonucleosomal chains into 300-A globular particles Jose L. Jorcano, Gunther Meyer, 6443-6447 [chromatin superstructure/histone HI (H5) interactions/reconstitution/ Loren A. Day, and Manfred Renz electron microscopy] Recombination between higher plant DNA and the Ti plasmid of M. F. Thomashow, R. Nutter, K. 6448-6452 tumefaciens (genomic DNA cloning/crown gall tumors) Postle, M.-D. Chilton, F. R. Blattner, A. Powell, M. P. Gordon, and E. W. Nester Site exclusion and sequence specificity in binding of 9-aminoacridine to the Peter R. Young and Neville R. 6453-6457 deoxytetranucleotide dpApGpCpT (intercalation/NMRlmultiple Kallenbach equilibria) Calcium control of the intestinal microvillus : Its implications for John R. Glenney, Jr., Anthony 6458-6462 the regulation of microfilament organizations (actin/villin/calmodulin/ Bretscher, and Klaus Weber membranes/trifluoperazine) Methylation of foreign DNA sequences in eukaryotic cells (DNA-mediated Yaakov Pollack, Reuven Stein, 6463-6467 transfer/thymidine kinase/5-methylcytosine/Southern blotting) , and The inverted repeat as a recognizable structural feature in supercoiled DNA David M. J. Lilley 6468-6472 molecules (Si single-strand-specific nuclease/CoIEl, pBR322, and OX1 74 replicative form DNA conformation/nucleic acid protein recognition) High-performance liquid chromatography technique for resolving multiple Alvin N. Kotake and Yoshihiko Funae 6473-6475 forms of hepatic membrane-bound cytochrome P-450 (high-performance liquid chromatography of rat hepatic microsomes/solubilized membrane protein/selective induction of cytochrome P-450) Nucleotide sequence of a cyanobacterial nifH gene coding for nitrogenase Moshe Mevarech, Douglas Rice, and 6476-6480 reductase (nitrogen fixation/Anabaena/protein sequence homologies) Robert Haselkorn Characterization of slow reacting substances (SRSs) of rat basophilic leukemia Dai-Eun Sok, Jin-Keon Pai, Vince 6481-6485 (RBL-1) cells: Effect of on SRS Atrache, and Charles J. Sih cysteinylglycine/transpeptidase/cysteine)cysteine profile (aminopeptidase/basophil/ Conformational flexibility of DNA: Polymorphism and handedness (DNA Goutam Gupta, Manju Bansal, and V. 6486-6490 structure/right-handed duplexes/left-handed duplexes/RL models) Sasisekharan Construction and analysis of simian virus 40 origins defective in tumor antigen Richard M. Myers and Robert Tjian 6491-6495 binding and DNA replication (recombinant plasmids/mutagenesis in vitro/ D2 protein) Three-dimensional structure of a protein from scorpion venom: A new Juan C. Fontecilla-Camps, Robert J. 6496-6500 structural class of neurotoxins (sodium channels/membranes/crystal Almassy, Fred L. Suddath, Dean D. structure/protein conformation/amino acid sequence) Watt, and Charles E. Bugg Mechanism of action of glucagon on hepatocyte phosphofructokinase activity Thomas H. Claus, John R. Schlumpf, 6501-6505 (ATP:D-fructose-6-phosphate 1-phosphotransferase/phosphorylationI M. Raafat El-Maghrabi, Jo Pilkis, fructose bisphosphate/enzyme regulation/pyruvate kinase) and Simon J. Pilkis Increased levels of (2'-5')oligo(A) polymerase activity in human lymphoblastoid IndiraKrishnan and Corrado Baglioni 6506-6510 cells treated with glucocorticoids (interferon-induced enzymes/ hydrocortisone/cortexolone/growth inhibition) Isolation and characterization of the mouse metallothionein-I gene Diane M. Durnam, Fabienne Perrin, 6511-6515 (recombinant DNA/DNA sequence/heteroduplex mapping/cadmium) Frank Gannon, and Richard D. Palmiter Purification of the regulatory component of adenylate cyclase (adenosine 3',5'- John K. Northup, Paul C. Sternweis, 6516-6520 cyclic monophosphate/hormone receptors/reconstitution/guanine Murray D. Smigel, Leonard S. nucleotide) Schleifer, Elliott M. Ross, and Alfred G. Gilman Identification of two Escherichia coli factor Y effector sites near the origins of S. L. Zipursky and K. J. Marians 6521-6525 replication of the plasmids ColEl and pBR322 (site specificity/ATP hydrolysis/initiation of DNA synthesis) Purification and properties of the double-stranded RNA-activated eukaryotic Haim Grosfeld and 6526-6530 initiation factor 2 kinase from rabbit reticulocytes (polypeptide chain initiation/protein phosphorylation/ternary complex formation/ translational control by double-stranded RNA) Mass spectra of partial protein hydrolysates as a multiple phase check for long Walter C. Herlihy, Nancy J. Royal, K. 6531-6535 polypeptides deduced from DNA sequences: NH2-terminal segment of Biemann, Scott D. Putney, and alanine tRNA synthetase (gas chromatographic mass spectrometry/protein Paul R. Schimmel sequencing/DNA sequencing) Homology exists among the transforming sequences of avian and feline sarcoma M. Shibuya, T. Hanafusa, H. 6536-6540 ( transformation/src gene/hybridization) Hanafusa, and J. R. Stephenson Isolation of yeast with mRNA levels controlled by phosphate Richard A. Kramer and Nancy 6541-6545 phosphatase/tranlscriptionconcentration (gene cloning/differentialregulation/multipleplaque genes)hybridization/acid Andersen

iii Contents

Use of an indicator sequence of human DNA to study DNA damage by Steven M. Grunberg and William A. 6546-6550 methylbis(2-chloroethyl)amine (nitrogen mustard//DNA Haseltine damage) Evidence for a 3-0-sulfated D-glucosamine residue in the antithrombin-binding Ulf Lindahl, Gudrun Bdckstrom, 6551-6555 sequence of heparin (anticoagulant activity/nitrous acid deamination/ Lennart Thunberg, and Irwin G. heparin octasaccharide/periodate oxidation/disaccharide units in heparin) Leder Escherichia coli RNA polymerase in vitro mimics simian virus 40 in vivo Edward B. Jakobovits, Sentob 6556-6560 transcription when the template is viral nucleoprotein (simian virus 40 Saragosti, Moshe Yaniv, and Yosef minichromosome/transcriptional control/transcriptional initiation/simian Aloni virus 40 late promoters) Structure and multiplicity of genes for the human immunoglobulin heavy chain Gaston Matthyssens and Terence H. 6561-6565 variable region (JH joining segment/ diversitylintervening Rabbitts sequence/gene rearrangement) Expression of a DNA strand initiation sequence of CoIE1 plasmid in a single- Nobuo Nomura and Dan S. Ray 6566-6570 stranded DNA phage (rifampicin-resistant initiation/dnaB/dnaG/phage M13) A theoretical study of the structure of parathyroid hormone (Correction) James E. Zull and Naomi B. Lev 6941 (protein domains/hydrophobicity/secondary structure/calcium metabolism) BIOPHYSICS Exchange kinetics of the Schiff base proton in bacteriorhodopsin (purple Benjamin Ehrenberg, Aaron Lewis, 6571-6573 membrane/halobacteria/proton pump/resonance Raman spectroscopy/ Thomas K. Porta, John F. Nagle, Schiff base pKa) and Walther Stoeckenius Iron-sulfur : Spin-coupling model for three-iron clusters (ferredoxins/ T. A. Kent, B. H. Huynh, and E. 6574-6576 exchange interactions/Mossbauer spectroscopy) Muinck Proton longitudinal relaxation investigation of histidyl residues of normal Irina M. Russu and Chien Ho 6577-6581 human adult and sickle deoxyhemoglobin: Evidence for the existence of pregelation aggregates in sickle deoxyhemoglobin solutions (NMR/sickle cell anemia/gelation) Kinetic models suggest bimolecular reaction steps in axonal Na+-channel gating Peter L. Dorogi and Eberhard 6582-6586 (ligand binding/conformational changes/acetylcholine/K* channels) Neumann Lateral distribution and diffusion of the C3b receptor of complement, HLA Howard R. Petty, Lloyd M. Smith, 6587-6591 antigens, and lipid probes in peripheral blood leukocytes (photobleaching/ Douglas T. Fearon, and Harden M. patching/fluorescence/monoclonal antibody) McConnell BOTANY Cytokinin inhibition of respiration in mitochondria from six plant species Carlos 0. Miller 6941 (Correction) (6-benzylaminopurine/ADPstimulation/alternative respiration/malate) CELL Identification of functional domains of human erythrocyte spectrin (peptide Jon S. Morrow, David W. Speicher, 6592-6596 maps/cytoskeleton/membrane binding/peptide association/protein William J. Knowles, C. Jennifer structure) Hsu, and Vincent T. Marchesi Prostaglandin 12 modifies both prolactin binding capacity and fluidity of mouse J. R. Dave and R. A. Knazek 6597-6600 liver membranes (prolactin receptors) Anionic lipid domains: Correlation with functional topography in a mammalian Elaine L. Bearer and Daniel S. Friend 6601-6605 (polymyxin B/plasma membrane/phospholipids/ spermatozoa/membrane protrusions) Active proliferation of Rous sarcoma virus-infected, but not normal, chicken Samuel D. Balk 6606-6610 heart mesenchymal cells in culture medium of physiological composition (chicken pectoral and chicken embryo fibroblasts/capillary endothelial cells/autonomy/growth factors) Growth of normal human glial cells in a defined medium containing platelet- C.-H. Heldin, A. Wasteson, and B. 6611-6615 derived growth factor (cell multiplication/serum-free conditions/MCDB Westermark 105 medium/miniclones) Control of serum protein production in hepatocyte hybridomas: Josiane Szpirer, Claude Szpirer, and 6616-6620 Immortalization and expression of normal hepatocyte genes (a-fetoprotein/ Jean-Claude Wanson gene mapping/mitosis control/insulin) Somatic cell genetic analysis of regulation of expression of 3-hydroxy-3- Michael Sinensky, Susan Armagast, 6621-6623 methylglutaryl-coenzyme A reductase (25-hydroxycholesterol resistance/ Gail Mueller, and Robert Torget mevalonate auxotrophy)

iv Contents Cytoskeletal F-actin patterns quantitated with fluorescein isothiocyanate- M. Verderame, D. Alcorta, M. Egnor, 6624-6628 phalloidin in normal and transformed cells (simianvirus 40/tumor antigens! K. Smith, and R. Pollack revertants/actin antibody/two-color fluorescence microscopy) Monoclonal to sucrase/isomaltase: Probes for the study of postnatal Hans-Peter Hauri, Andrea Quaroni, 6629-6633 development and biogenesis of the intestinal microvillus membrane (fetal and Kurt J. Isselbacher intestinal transplantation/enzyme precursor/immunofluorescence/cell differentiation) Tissue-specific DNA methylation in a cluster of rabbit (3-like globin genes (gene Che-Kun James Shen and Tom 6634-6638 mapping/molecuslar cloning/gene expression) Maniatis Autonomous movements of cytoplasmic fragments (amoeboid motion/ Guenter Albrecht-Buehler 6639-6643 cytoplasm/movement control) Sequential addition of platelet factor and plasma to BALB/c 3T3 fibroblast David R. Clemmons, Judson J. Van 6644-6648 cultures stimulates somatomedin-C binding early in (growth Wyk, and W. J. Pledger factor/receptors/fibroblast growth factorlreplication) Antipain inhibits N-methyl-N'-nitro-N-nitrosoguanidine-induced Joseph A. DiPaolo, Suzanne C. 6649-6653 transformation and increases chromosomal aberrations (DNA replications Amsbaugh, and Nicholae C. protease inhibitor/mitotic recombination/sister chromatid exchange/ Popescu carcinogenesis) Induction of growth in kidney epithelial cells in culture by Na+ (BSC-1 cells/ F. Gary Toback 6654-6656 initiation of DNA synthesis/serum/Li) Visualization of microtubules of cells in situ by indirect immunofluorescence H. Randolph Byers, Keigi Fujiwara, 6657-6661 ( organizing centers/cytoskeleton/chromatophores/ and Keith R. Porter scleroblasts/orthogonally arranged collagen) Glycosaminoglycans that bind cold-insoluble globulin in cell-substratum John Laterra, Riva Ansbacher, and 6662-6666 adhesion sites of murine fibroblasts (fibronectin/heparan sulfate/ Lloyd A. Culp hyaluronic acid/affinity chromatography/simian virus 40-transformed cells) Clone-specific cellular recognition in a sea anemone (nematocytes/allogeneic Roger Lubbock 6667-6669 recognition/cell surface markers) Regulatory coupling of nitrate and sulfate assimilation pathways in cultured Ziva Reuveny, Donald K. Dougall, and 6670-6672 cells [nitrate reductase (NADH)/sulfate adenylyltransferase/ATP Philip M. Trinity sulfurylase/positive feedback regulation/nitrogen and sulfur metabolism] Endogenous electrical currents in the water mold Blastocladiella emersonii Robert F. Stump, Kenneth R. 6673-6677 during growth and sporulation (ion currents/development/membrane Robinson, Ruth L. Harold, and transport/protons/calcium) Franklin M. Harold Cellulose biogenesis: Polymerization and crystallization are coupled processes in Moshe Benziman, Candace H. 6678-6682 Acetobacter xylinum (Calcofluor White/microfibril assembly/hydrogen Haigler, R. Malcolm Brown, Jr., bonding) Alan R. White, and Kay M. Cooper Mutant of avian erythroblastosis virus defective for erythroblast Hartmut Beug, Gay Kitchener, Gabi 6683-6686 transformation: Deletion in the erb portion of p75 suggests function of the Doederlein, Thomas Graf, and protein in leukemogenesis (target cells/cell transformation/gag-related Michael J. Hayman proteins) Altered distributions of the cytoskeletal proteins vinculin and a-actinin in Therbse David-Pfeuty and S. J. Singer 6687-6691 cultured fibroblasts transformed by Rous sarcoma virus (cell adhesion/ immunofluorescence/interference reflection microscopy/oncogenic viruses) Receptor-mediated internalization of fluorescent gonadotropin-releasing Eli Hazum, , Jan 6692-6695 hormone by pituitary gonadotropes (fluorescence microscopy/hormone Marian, and P. Michael Conn receptors/patching/endocytosis/receptor microaggregation) Subcompartments of the G1 phase of cell cycle detected by flow cytometry Zbigniew Darzynkiewicz, Thomas 6696-6699 (RNA content/cell quiescence/cell proliferation/indeterminate and Sharpless, Lisa Staiano-Coico, and deterministic states of GI) Myron R. Melamed Transepithelial migration of human neutrophils: An in vitro model system Eva B. Cramer, Linda C. Milks, and 6941 (Correction) (leukocytes/chemotaxis/cell-cell interaction/intercellular George K. Ojakian junctions) Interferon inhibits the conversion of 3T3-L1 mouse fibroblasts into adipocytes Susay Keay and Sidney E. Grossberg 6941 (Correction) (cell differentiation/insulin/antiviral activity) IEVELOPMENTAL BIOLOGY Synthesis of a posterior indicator protein in normal embryos and double Herbert Jdckle and Klaus Kalthoff 6700-6704 abdomens of Smittia sp. (Chironomidae, Diptera) (cytoplasmic determinants/localization/differential gene expression/morphogenesisl photoreversible UV effect) Presumptive common precursor for neuronal and glial cell lineages in mouse F. De Vitry, R. Picart, C. Jacque, L. 6941 hypothalamus (Correction) (immunocytochemistry/ventricular cell/S-100 Legault, P. Dupouey, and A. Tixier- protein/differentiation) Vidal v Contents

GENETICS Asynchronous replication of heterochromatin in maize (B / A. Pryor, K. Faulkner, M. M. 6705-6709 nondisjunction/late replication) Rhoades, and W. J. Peacock Genetic variability and effective population size when local extinction and Takeo Maruyama and Motoo Kimura 6710-6714 recolonization of subpopulations are frequent (population /protein polymorphism/periodic selection/neutral mutation-random drift hypothesis) Maternal inheritance of human mitochondrial DNA (genetic polymorphism/ Richard E. Giles, Hugues Blanc, 6715-6719 restriction endonuclease cleavage map/blood platelets) Howard M. Cann, and Douglas C. Wallace Synaptonemal complexes at premeiotic interphase in the mouse spermatocyte R. F. Grell, E. F. Oakberg, and E. E. 6720-6723 (DNA replication/synapsis/recombination/electron microscopy/meiosis) Generoso Sequence-specific recombination of plasmid ColEl (conjugal transmission/ Gareth J. Warren and Alvin J. Clark 6724-6728 recA-independent recombination/Escherichia coli) Circadian rhythm mutations in Drosophila melanogaster affect short-term C. P. Kyriacou and Jeffrey C. Hall 6729-6733 fluctuations in the male's courtship song (interpulse interval/per mutations/diplo-X transformed males) Evolutionary approach to the question of immunoglobulin heavy chain J. V. Ravetch, I. R. Kirsch, and P. 6734-6738 switching: Evidence from cloned human and mouse genes (recombination/ Leder DNAlantibodies) Replication and meiotic transmission of yeast ribosomal RNA genes Bonita J. Brewer, Virginia A. Zakian, 6739-6743 (Saccharomyces cerevisiae/multiple-copy genes/sequence homogeneity/ and Walton L. Fangman meiosis) Initiation of DNA replication in a ColEl-type plasmid: Isolation of mutations in Satoshi Naito and Hisao Uchida 6744-6748 the ori region (X-mini-ColE1 hybrid phage/DNA sequence/revertants/ hairpin structure) Immunoreactive helix-destabilizing protein localized in transcriptionally active Gordhan L. Patel and Peter E. 6749-6753 regions of Drosophila polytene chromosomes (indirect Thompson immunofluorescence/ puffs/heat shock response) A highly polymorphic locus in human DNA (human genetics/recombinant Arlene R. Wyman and Ray White 6754-6758 DNA/genetic marker loci) Expression of an X-linked gene from an inactive human X chromosome in T. Mohandas, R. S. Sparkes, B. 6759-6763 mouse-human hybrid cells: Further evidence for the noninactivation of the Hellkuhl, K. H. Grzeschik, and L. J. steroid sulfatase locus in man (X-linked ichthyosis/X-chromosome Shapiro abnormalities) IMMUNOLOGY Initial characterization of monoclonal antibodies against human monocytes Valentina Ugolini, Gabriel Nunez, R. 6764-6768 (lymphocyte hybridoma/monocyte antigens) Graham Smith, Peter Stastny, and J. Donald Capra Antiviral response elicited by a completely synthetic antigen with built-in , , Monique 6769-6772 adjuvanticity (synthetic adjuvant/coliphage MS-2/viral coat protein/ Parant, and Louis Chedid nonspecific immunity) Resistance of simian virus 40-transformed hamster cells to the cytolytic effect of James L. Cook, John B. Hibbs, Jr., 6773-6777 activated macrophages: A possible factor in species-specific viral and Andrew M. Lewis, Jr. oncogenicity (viral transformation/tumor antigens/tumor immunity) Separation of antibody helper and antibody suppressor human T cells by using Yair Reisner, Savita Pahwa, J. W. 6778-6782 soybean agglutinin (human lymphocyte subpopulation/differential Chiao, Nathan Sharon, Robert L. agglutination) Evans, and Robert A. Good Murine T cell Ia antigens: Specificity Iat.46 maps in I-J to I-E chromosomal Colleen E. Hayes and John Michael 6783-6787 segment of H-2 complex (I region/alloantigen/lymphocyte/Ir genes) Maier Production of auto-anti-idiotypic antibody during the normal immune response: Edmond A. Goidl, G. Jeanette 6788-6792 Changes in the auto-anti-idiotypic antibody response and the idiotype Thorbecke, Marc E. Weksler, and repertoire associated with aging (immune network/immune regulation/ Gregory W. Siskind antibody expression/age effects) Simultaneous expression of mouse immunoglobulins M and D is determined by Matthias R. Wabl, Judith P. Johnson, 6793-6796 the same homolog of chromosome 12 (membrane proteins/Syrian hamster Ingrid G. Haas, Maria Tenkhoff, cell hybrid/acid phosphatase 1 isoelectric focusing) Tommaso Meo, and Regine Inan Anti-gamete monoclonal antibodies synergistically block transmission of Joan Rener, Richard Carter, Yvonne 6797-6799 malaria by preventing fertilization in the mosquito (malaria gamete Rosenberg, and Louis H. Miller antigens/hybridoma/agglutination) Role of adenylate cyclase in immunologic release of mediators from rat mast Stephen T. Holgate, Robert A. Lewis, 6800-6804 cells: Agonist and antagonist effects of purine- and ribose-modified and K. Frank Austen adenosine analogs (protein kinase/R and P sites/secretion/cyclic AMP) vi Contents

High molecular weight antigens present on human T cells (crossreacting Warren Judd, Clifton A. Poodry, 6805-6809 antigens/glycoprotein family/glycosylation processing) Samuel Broder, Steven M. Friedman, Leonard Chess, and Jack L. Strominger MEDICAL SCIENCES Strains and clones of Trypanosoma cruzi can be characterized by pattern of C. Morel, E. Chiari, E. Plessmann 6810-6814 restriction endonuclease products of kinetoplast DNA minicircles (Chagas Camargo, D. M. Mattei, A. J. disease/rapid method for kinetroplast DNA isolation/schizodemesi Romanha, and L. Simpson zymodemes) T-cell lines established from human T-lymphocytic neoplasias by direct Bernard J. Poiesz, Francis W. 6815-6819 response to T-cell growth factor (mycosis fungoides/Sezary syndrome/acute Ruscetti, James W. Mier, Andrea lymphocytic leukemia) M. Woods, and Robert C. Gallo A cardioactive factor in human plasma with positive inotropic and positive Sumner M. Kalman and Ruth M. 6820-6821 chronotropic activity (endogenous cardioactive substance) Jones Sanfilippo disease type D: Deficiency of N-acetylglucosamine-6-sulfate Hans Kresse, Eduard Paschke, Kurt 6822-6826 sulfatase required for heparan sulfate degradation (mucopolysaccharidosis/ von Figura, Walter Gilberg, and keratan sulfate/lysosomes) Walburga Fuchs Primary structure of ovine hypothalamic somatostatin-28 and somatostatin-25 Fred Esch, Peter Bohlen, Nicholas 6827-6831 (prohormone/Edman degradation/tryptic map/high-performance liquid Ling, Robert Benoit, Paul Brazeau, chromatography) and Roger Guillemin Structural analysis of Factor VIII antigen in von Willebrand disease (von Ralph L. Nachman, Eric A. Jaffe, 6832-6836 Willebrand factor heterogeneity/hemostasis/radioactive peptide maps) Connie Miller, and W. Ted Brown Production and release of plasminogen by isolated perfused rat liver Hidehiko Saito, Stewart M. Hamilton, 6837-6840 (perfluorochemical perfusate/cycloheximide/lysine-agarose/ Anthony S. Tavill, Loizos Louis, antiplasminogen antiserum/double-antibody precipitation) and Oscar D. Ratnoff Generation of human monoclonal antibodies reactive with human mammary Jeffrey Schlom, David Wunderlich, 6841-6845 carcinoma cells (breast /hybridomas/tumor antigens) and Yoshio A. Teramoto Vascular relaxing activity and stability studies of 10,10-difluoro-13,14- Yoshio Hatano, Jai D. Kohli, Leon I. 6846-6850 dehydroprostacyclin (prostaglandin I2/stable analog/isolated blood vessels/ Goldberg, Josef Fried, and Mukund canine mesenteric artery/bovine coronary artery) M. Mehrotra Nucleic acid spot hybridization: Rapid quantitative screening of lymphoid cell Janet Brandsma and George Miller 6851-6855 lines for Epstein-Barr viral DNA (nucleic acid hybridization/Epstein-Barr virus/lymphocytes) Localization of anti-mitochondrial antibody in experimental canine myocardial James T. Willerson, P. Kulkarni, 6856-6859 infarcts (myocardial scintigraphy/cell membrane permeability/irreversible Marvin Stone, Samuel E. Lewis, cellular damage) Edwin Eigenbrodt, Frederick J. Bonte, Robert W. Parkey, and L. Maximilian Buja Isolation and characterization of the amyloid-related apoprotein (SAA) from Nils Eriksen and Earl P. Benditt 6860-6864 human high density lipoprotein (amino acid composition/NH2-terminal amino acid sequence/isoelectric point/molecular weight/protein AA) Deoxycytidine kinase-mediated toxicity of deoxyadenosine analogs toward Dennis A. Carson, Donald B. Wasson, 6865-6869 malignant human lymphoblasts in vitro and toward murine L1210 leukemia Jonathan Kaye, Buddy Ullman, in vivo (adenosine deaminase/chemotherapy) David W. Martin, Jr., Roland K. Robins, and John A. Montgomery Relationship of biosynthesis of slow reacting substance to intracellular Charles W. Parker, Charles M. 6870-6873 glutathione concentrations (glutathione peroxidase/hydroperoxides/ Fischman, and H. James Wedner leukotrienes/icosanoic acids/glutathione transferase) Proportion of hemoglobin G Philadelphia (a268 A8n-LY8/32) in heterozygotes is Gwendolyn B. Sancar, Basil Tatsis, 6874-6878 determined by a-globin gene deletions (restriction endonuclease mapping/ Marisol M. Cedeno, and Ronald F. a-thalassemia/American Blacks/hemoglobin variants) Rieder MICROBIOLOGY DNA gyrase on bacterial chromosome: Possibility of two levels of action Karl Drlica, Elizabeth C. Engle, and 6879-6883 (oxolinic acid/DNA synthesis/recA mutant) Stephen H. Manes NEUROBIOLOGY Molecular characterization, reconstitution, and "transport-specific Stanley M. Goldin, Victoria Rhoden, 6884-6888 fractionation" of the saxitoxin binding protein/Na+ gate of mammalian and Emma Jean Hess brain (excitable membranes/artificial lipid vesicles/action potential/ purification)

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Studies with [35Sjmethionine indicate that the 22,000-dalton [MetJenkephalin- Jean Rossier, Jose M. Trifar6, 6889-6891 containing protein in chromaffin cells is a precursor of [Metlenkephalin Randolph V. Lewis, Raymond W. (immunoprecipitation/adrenal/prohormone/) H. Lee, Alvin Stern, Sadao Kimura, Stanley Stein, and Sidney Udenfriend Biochemical characterization of putative central purinergic receptors by using Michael Williams and Edwin A. 6892-6896 2-chloro[3Hfadenosine, a stable analog of adenosine (radioligand binding! Risley central neuromodulators/methylxanthines) Involvement of calcium channels in short-term desensitization of muscarinic Esam El-Fakahany and Elliott 6897-6901 receptor-mediated cyclic GMP formation in mouse neuroblastoma cells Richelson (manganese ion/histamine HI receptors/cyclic nucleotides) Schwann cells proliferate but fail to differentiate in defined medium Fernando Moya, Mary B. Bunge, and 6902-6906 (peripheral nerve development/extracellular matrix/myelination/nerve Richard P. Bunge tissue culture) Regulation by secretin, vasoactive intestinal peptide, and somatostatin of cyclic Dietrich van Calker, Margarete 6907-6911 AMP accumulation in cultured brain cells (glioblasts/peptides/adenylate Muller, and Bernd Hamprecht cyclase/receptors/gastrointestinal hormones) Mechanism of calcium current modulation underlying presynaptic facilitation Marc Klein and Eric R. Kandel 6912-6916 and behavioral sensitization in Aplysia (learning/synaptic plasticity! serotonin/cyclic AMP/potassium currents) Distinct cholecystokinin receptors in brain and pancreas (receptor binding/ Robert B. Innis and Solomon H. 6917-6921 gastrin/guanyl regulation) Snyder Dependence of in vitro myogenesis on a trophic protein present in chicken Tae Hwan Oh and George J. 6922-6925 embryo extract (trophic influence/muscle differentiation) Markelonis Synpatic endings and their postsynaptic targets in neostriatum: Synaptic Philip M. Groves 6926-6929 specializations revealed from analysis of serial sections (dopamine/5- hydroxydopamine/rats/basal ganglia) Single channel currents from excised patches of muscle membrane Richard Horn and Joseph Patlak 6930-6934 (acetylcholine channel/myotubes/mammalian musclelionic currentsl perfusion) PHYSIOLOGICAL SCIENCES Calcitonin, a major gill hormone (calcium efflux/phosphate efflux/calcium G. Milhaud, L. Bolis, and A. A. 6935-6936 homeostasis/flow rate/active transport) Benson POPULATION BIOLOGY Alternative male life histories in bluegill sunfish (sexual selection/breeding Mart R. Gross and Eric L. Charnov 6937-6940 systems/parental carelcuckoldry/frequency-dependent selection)

Corrections BIOCHEMISTRY A theoretical study of the structure of parathyroid hormone (protein domains/ James E. Zull and Naomi B. Lev 6941 hydrophobicity/secondary structure/calcium metabolism) BOTANY Cytokinin inhibition of respiration in mitochondria from six plant species (6- Carlos 0. Miller 6941 benzylaminopurine/ADPstimulation/alternative respiration/malate) Transepithelial migration of human neutrophils: An in vitro model system Eva B. Cramer, Linda C. Milks, and 6941 (leukocytes/chemotaxis/cell-cell interaction/intercellular junctions) George K. Ojakian Interferon inhibits the conversion of 3T3-L1 mouse fibroblasts into adipocytes Susan Keay and Sidney E. Grossberg 6941 (cell differentiation/insulin/antiviral activity) DEVELOPMENTAL BIOLOGY Presumptive common precursor for neuronal and glial cell lineages in mouse F. De Vitry, R. Picart, C. Jacque, L. 6941 hypothalamus (immunocytochemistry/ventricular cell/S-100 protein! Legault, P. Dupouey, and A. Tixier- differentiation) Vidal

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