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Regulation of Cyclins During the Cell Cycle

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Regulation of Cyclins During the Cell Cycle Regulation of cyclins during the cell cycle G1/S phase : Inhibitory Replicative senescence P Replicative senescence phosphorylation Differentiation TGFβ p53 Hormones P : Activating phosphorylation UV stress CDK7 Cyclin-activating p19INK4D p57Kip2 kinase (CAK) p18INK4C Cyclin H p27Kip1 p14ARF INK4A Cip p16 P p21 P or p15INK4B INK4 CDK4/6 CDK2 Family Cip/Kip Family – Cyclin A Cyclin D Cyclin E – Cyclin E P P Mitogen GSK3β – CDK1 withdrawal CDC25A Cyclin E – Polymerase α – PCNA Inhibition of Mitogens MAPKs transcription P P S phase genes P P Rb Rb P P Cyclin D c-Jun c-Fos DP1 E2F DP1 E2F Beta-TRCP G2/M phase CDC25A/B/C Wee1 Bim Myt1 P Dephosphorylation Positive feedback loop P P P Proteasomal P P Wee1 P P degradation CDK1 CDK1 P P CDK1 P Cyclin B Cyclin B Cyclin B Myt1 CDK7 P P P P CENP-F Increased Cyclin B Cyclin H PLK1 APC/C Aurora B protein levels P APC/C CDK1 CDC20 Cyclin B Ub Cyclin B Ub Metaphase Spindle assembly Ub proteasomal degradation Anaphase plate alignment checkpoint extinguished progression Antibodies Product Application Species reactivity AbID Aurora B antibody ICC/IF, IHC-Fr, IHC-P, IP, WB Hm, Hu, Ms, Pig, Rt ab2254 Bim antibody [Y36] Flow Cyt, ICC/IF, IHC-FoFr, IHC-P, IP, WB Hu, Ms, Rt ab32158 Cdk2 antibody [E304] Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rt ab32147 Cdk4 antibody [EPR4513-32-7] Flow Cyt, ICC/IF, IHC-P, WB Hu ab108357 CDKN2A/p16INK4a antibody [EPR1473] Flow Cyt, ICC/IF, IHC-P, IP, WB Hu ab108349 c-Fos antibody [EPR883(2)] Flow Cyt, WB Hu, Ms, Rt ab134122 c-Jun antibody [E254] ICC/IF, IHC-P, IP, WB Hu, Ms, Rt ab32137 Cyclin A2 antibody [E142] ICC/IF, IHC-P, WB Hu, Ms, Rt ab181591 Cyclin B1 antibody [Y106] Flow Cyt, ICC, IHC-P, IP, WB Hu ab32053 Cyclin D1 antibody [EPR2241] ICC/IF, IHC-P, IP, WB Hu, Ms, Rt ab134175 Cyclin E1 antibody [EP435E] Flow Cyt, ICC/IF, IP, WB Hu ab33911 E2F1 antibody [EPR3818(3)] Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rt ab179445 GSK3 beta antibody [Y174] Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms ab32391 p21 antibody [EPR3993] WB AGMk, Hu, Ms, Rt ab109199 p27 KIP 1 antibody [Y236] Flow Cyt, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, IP, WB Hu, Rt ab32034 p53 antibody [DO-1] ChIP, ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu ab1101 p57 Kip2 antibody [EP2515Y] ICC/IF, IHC-P, IP, WB Hu, Ms, Rt ab75974 Rb antibody [EPR17512] Flow Cyt, ICC/IF, IHC-P, IP, WB AGMk, Hu, Ms ab181616 Inhibitors and activators Target Compound Description AbID CDK1 Ro 3306 Selective inhibitor. Ki of 35 and 110 nM for CDK1/Cyclin B1 and CDK1/Cyclin A. ab141491 CDK4/6 Palbociclib Potent, highly specific inhibitor. IC50 of 11 nM (CDK4) and 16 nM (CDK6). ab218118 GSK3β BIO Cell-permeable, selective, reversible inhibitor. IC50 of 5 nM (GSK3α/β). ab120891 p21 UC2288 p53-independent, selective p21 attenuator. GI50 of 10 μM. ab146969 p53 RITA Induces p53 activation in tumor cells. Prevents p53-HDM2 (MDM2) interaction. ab219379 Rb Silibinin Increases unphosphorylated Rb (active) 4-fold and increases Rb-E2F complexes. ab142948 Other related products Product Details and applications Species reactivity AbID CDK1 ELISA Kit 90-min ELISA kit made with recombinant monoclonal antibodies Hu ab212162 CDKN2A/p16INK4a protein SDS-PAGE - ab84075 GSK3 beta (pS9 + Total) ELISA Kit 90-min ELISA Hu, Ms, Rt (predicted) ab205711 GSK3 beta protein Functional studies, SDS-PAGE, western blot - ab60863 GSK3 beta pS9 ELISA Kit 90-min ELISA Hu, Ms, Rt (predicted) ab205709 GSK3 beta ELISA Kit 90-min ELISA Hu, Ms, Rt (predicted) ab205710 p21 ELISA Kit 90-min ELISA kit made with recombinant monoclonal antibodies Hu ab214658 p21 ELISA Kit 90-min ELISA kit made with recombinant monoclonal antibodies Ms ab205576 p21 protein Functional studies, SDS-PAGE, western blot - ab56278 p53 ELISA Kit 90-min ELISA Hu ab171571 p53 protein Functional studies, SDS-PAGE, western blot - ab43615 Rb protein Functional studies, SDS-PAGE - ab56270.
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