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Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 2000 The aE rly Local and Regional Immune Response to Recall Inoculations of Mycobacterium Leprae in the Skin of Rhesus Macaques (Macaca Mulatta). Thomas Xavier d Lemarchand Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Lemarchand, Thomas Xavier d, "The Early Local and Regional Immune Response to Recall Inoculations of Mycobacterium Leprae in the Skin of Rhesus Macaques (Macaca Mulatta)." (2000). LSU Historical Dissertations and Theses. 7157. https://digitalcommons.lsu.edu/gradschool_disstheses/7157 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bieedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9* black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. Bell & Howell Information and Learning 300 North Zeeb Road, Ann Arbor, Ml 48106-1346 USA 800-521-0600 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. THE EARLY LOCAL AND REGIONAL IMMUNE RESPONSE TO RECALL INOCULATIONS OF MYCOBACTERIUM LEPRAE IN THE SKIN OF RHESUS MACAQUES (MACACA MULATTA) A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Interdepartmental Program in Veterinary Medical Sciences Through the Department of Veterinary Pathology by Thomas Xavier D. Lemarchand DEDV, University of Nantes, France, 1992 DES vet path, University of Paris, 1993 May 2000 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. UMI Number 9963952 ___ ® UMI UMI Microform 9963952 Copyright 2000 by Bell & Howell Information and Learning Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. Bell & Howell Information and Learning Company 300 North Zeeb Road P.O. Box 1346 Ann Arbor, Ml 48106-1346 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. DEDICATION I dedicate this work to my brother Jerome who passed away from cancer while I was acquiring data to test the hypothesis of the study reported herein. The goals and philosophy of biomedical research to alleviate disease and suffering of humans and our fellow animals take their full and very concrete meanings when a beloved one, in the dawn of happiness and a new period of his life, is full blown by disease. ii Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. ACKNOWLEDGEMENTS I wish to acknowledge my major professor, Dr David Scollard, for his communicating enthusiasm concerning leprosy research. He has the most important qualities that I was anticipating before starting this research project. His immuno-pathological expertise and his experience in this area of research were invaluable. His understanding, kindness and support were also invaluable during the many hard times of this work. My thanks are also directed to my committee members, Dr Gary Baskin, Dr Doo-Youn Cho, Dr Tom Gillis, Dr Kathy O’Reilly and Dr Roger Laine, who always had valuable advises and constructive comments. I feel gifted and lucky to have benefited from such a quality of scientific advisers. This project was also a prime example of collaboration and I would like to warmly thank the persons and teams that were involved. First the kind and warm people from the Laboratory Research Branch of he Gillis W. Long Hansen’s Disease Center where I did this research project. Among them, Joe Allen, Angelina Deming, Gregory McCormick, Melvyn Morales and Felipe Sandoval were always ready to give their help, time and expertise for this project to be more doable. Also the people from Dr Tom Gillis’ and Dr Diana Williams’ laboratory, in particular Laynette Spring, Sheryl Lewis and Naoko Robbins, who welcomed me in their “kingdom” and were always available to answer my questions. Second, the team of the Tulane’s Delta Primate Research Center at Covington, Louisiana, in particular Dr Marion Ratterree who performed the sampling on the rhesus monkeys, and the animal caretakers of the center. I also would like to thank Dr Bobby Gormus, without the monkeys of whom this project would never have been possible, and for the scientific interaction we had when trying to interpret our results and anticipate future directions for investigations. Dr Gary Baskin took care of the main study end-point and his contribution was critical to a second level of interpretation of my database, adding an unexpected, yet very interesting scientific meaning to this study. Third, several persons from the Department of Veterinary Microbiology and Parasitology also were very helpful for an easier completion of this work. Marilyn Dietrich acquired the data for the flow cytometry study and was very helpful and knowledgeable interlocutor when discussing flow cytometry iii Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. issues. Chad, who was working in Dr David Horohov’s laboratory, very nicely helped me troubleshooting technical problems encountered during molecular biology procedures. Dr David Horohov was also present for constructive discussions in the field of the reverse transcription and polymerase chain reaction technology. Lastly, Dr Elmer Godeny was always ready for logistic collaboration and we helped each other several times for sampling of rhesus monkey peripheral blood. Lastly, Michael Kearney efficiently and fruitfully assisted me in the statistical analysis of the generated data. Harry Cowgill and Gregory McCormick helped me, the latter very substantially, in the processing of the illustrations. I am truly indebted to all those people. I would also like to thank Dr Henry Wayne Taylor, who gave me the opportunity to follow this combined doctoral and residency program and all the folks from the Department of Veterinary Pathology for their kindness and valuable help and scientific interactions. The knowledge and outstanding will to teach of the faculty members also helped me to achieve an even better level of expertise in the field of diagnostic veterinary pathology. Finally, and very importantly, I would like to thank my spouse, Christine, for constant support and understanding, the latter being greatly helped by her graduate student condition: she perfectly knew what I was talking about, except maybe when I was going into specialized technical details. Her interest for scholarship, adventures and discoveries had helped us to enjoy a life filled with hardship and rewards as well as discovery of oversea lands. My daughter Audrey, who was bom during this program, had also been quite “wise” for a toddler and 3 year-old to understand that sometimes, her “Dada” had to go working instead of playing with her. iv Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. TABLE OF CONTENTS Dedication ................................................................................................................................................ii Acknowledgements .................................................................................................................................iii List of Abbreviations..............................................................................................................................ix Abstract..................................................................................................................................................xii 1 INTRODUCTION__________________________________ 1 l . l Literature Cited .........................................................................................................................5 2 REVIEW OF THE LITERATURE ON THE IMMUNO-PATHOLOGY OF LEPROSY..... 7 2.1 Cell Mediated Immunity (CMI)

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