bioRxiv preprint doi: https://doi.org/10.1101/2020.10.22.351585; this version posted October 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 The Biogeography of Fungal Communities Across Different Chinese Wine- 2 producing Regions Associated with Environmental Factors and Spontaneous 3 Fermentation Performance 4 Ruilong Li,a Siyu Yang,a Mengyuan Lin,a Sijiang Guo,a Xiaoyu Han,a Mengmeng Ren,a Le 5 Du,b Yinghui Song,c Yilin You,a Jicheng Zhan,a# Weidong Huang,a# 6 aCollege of Food Science and Nutritional Engineering, Beijing Key Laboratory of Viticulture 7 and Enology, China Agricultural University, Beijing, 100083, China 8 bWuhan Donghu Big Data Trading Center Co. Ltd., Wuhan, 430200, China 9 cPenglai Grape and Wine Industry Development Service Center, Yantai, 265600, China 10 #Address correspondence to Weidong Huang,
[email protected]; Jicheng 11 Zhan,
[email protected]. 12 Abstract Chinese Marselan grapes are believed to possess the potential to become a characteristic 13 regional variety, whose quality is internationally recognized. The fermentation-related mycobiota 14 from six climatically diverse Marselan-producing regions in China were analyzed via high- 15 throughput sequencing (HTS), while the influence of environmental factors was evaluated as well. 16 The results implied that the phyla Ascomycota and genus Aureobasidium dominated the fungal 17 communities in 166 Marselan must and ferment samples. Significant differences were detected in 18 the fungal microbiota from the regions, as well as the wineries, while these discrepancies decreased 19 as the fermentation progressed.