1 Curriculum Vitae

Curriculum Vitae, Erin Ahn

Curriculum Vitae

Full name: Eun-Young Erin Ahn, Ph.D. Current Position Associate Professor Department of Biochemistry and Molecular Biology Mitchell Cancer Institute College of Medicine University of South Alabama Campus Address Permanent Address 1660 Springhill Ave. 1425 Oak Vale Ct. Mobile, AL 36604 Mobile, AL 36609 Telephone (Work) 1-251-445-9805 E-mail: [email protected]

Education & Training BS (1992-1996) Seoul National University Department of Biology Education Seoul, Korea MS (1996-1998) Seoul National University Department of Biology Education Seoul, Korea Thesis : Development of an overexpression system for beta-galactosidase in Escherichia coli using genetic elements of groEx. Ph.D. (1998-2003) University of Alabama at Birmingham Department of Pathology Molecular & Cellular Pathology Graduate Program Birmingham, AL (Advisor: Jay M. McDonald, M.D.) Thesis: Molecular mechanisms of apoptosis in human cholangiocarcinoma: Regulation by Fas, calcium/calmodulin and interferon-gamma. Postdoctoral Fellow (2004 - June 2009) Scripps Research Institute (2004 – Feb 2008) Department of Molecular and Experimental Medicine La Jolla, CA (Advisor : Dong-Er Zhang, Ph.D.) University of California San Diego (Mar 2008 – Jun 2009) Moores UCSD Cancer Center La Jolla, CA (Advisor: Dong-Er Zhang, Ph.D.) Assistant Project Scientist (July 2009 – June 2012) University of California San Diego Moores UCSD Cancer Center La Jolla, CA 1

Curriculum Vitae, Erin Ahn Professional Positions and Experience Assistant Professor (July 2012 – July 2016) Division of Oncologic Science Mitchell Cancer Institute University of South Alabama, Mobile, AL Assistant Professor (July 2012 – July 2016) Division of Oncologic Science Mitchell Cancer Institute University of South Alabama, Mobile, AL Associate Professor (with tenure; August 2016 – present) Division of Oncologic Science Mitchell Cancer Institute University of South Alabama, Mobile, AL Associate Professor (May 2019 - present) Department of Biochemistry and Molecular Biology College of Medicine University of South Alabama, Mobile, AL

Awards and Honors  RARE Champion of Hope Award Nominee, Global Genes (2019) https://globalgenes.org/rare-champion-of-hope-award-nominees/  Human Diseases Genes Website Series – Moderator for the SON gene (2019-present) https://humandiseasegenes.nl/son/ https://humandiseasegenes.nl/moderators/  Highlighted in News “MCI teams finds link to rare disorder”, (2017) http://www.checkorphan.org/news/mci-team-finds-link-to-rare-disorder  The Mayer Mitchell Award for Excellence in Cancer Research (2017)  Travel Award. 50th annual Meeting, American Society of Hematology (2008)  International Awards for Research in Leukemia and Allied Conditions, Lady TATA Memorial Trust, United Kingdom. Postdoctoral fellowship (2005-2007)  Best Research Presentation Award. Moores UCSD Cancer Center Annual Retreat (2007)  The John R. Durant Award for the Excellence in Cancer Research. First Place in Graduate Student Category. Comprehensive Cancer Center, University of Alabama at Birmingham (2001)  College Dean Award (1st honor) for the excellence in undergraduate study. Seoul National University, Seoul, Korea (1996)

Membership  Member, American Association for Cancer Research  Member, American Society of Hematology  Member, American Society of Human Genetics

Curriculum Vitae, Erin Ahn Academic Service

Reviewer, NIH CSR Study Sections  NIH CAMP Study Section (February 2017)  NIH CAMP Study Section (June 2017)  NIH MONC Study Section (October 2017)  NIH/NCI Special Emphasis Panel ZCA1 RPRB-F (O1) for P01 review (June 2017)  NIH Special Emphasis Panel/Scientific Review Group ZRG1 F09B-M (20) (November 2018)  NIH Cancer Genetic (CG) Study Section (June 2019)  NIH Special Emphasis Panel/Scientific Review Group ZRG1 F09B-M (20) L (July 2019)  NIH Cancer Genetic (CG) Study Section (October 2019)

Other academic service  Member, Mitchell Cancer Institute Biosafety Committee  Member, College of Medicine Flow Cytometry Facility Advisory Committee  Editorial Board, Journal of Cancer Research and Therapeutic Oncology,  Editorial Board, Journal of Cancer Diagnosis  Reviewer, Abraham Mitchell Cancer Research Award Grant (2013)  Ad hoc Reviewer for journals: Cell Reports, Blood, Leukemia Research, Leukemia and Lymphoma, PLoS One, Molecular and Cellular Biology, British Journal of Cancer, International Journal of Molecular Sciences, Journal of cancer Diagnosis, Journal of Cancer Sciences.  Organizing Assistant for The18th International RUNX Workshop, San Diego, CA (2011)

Community service and other extramural activities

 Touch-A-Truck Pediatric Cancer Research fund-raising event committee (2017- present)  Mitchell Cancer Institute representative. Women in STEM Teen Conference and Career Expo, Alabama School of Math and Science, AL (2017)

Research Interests  SON-mediated RNA splicing and epigenetic regulation of transcription in normal hematopoiesis and leukemia  RNA-binding proteins (SON, RBFOX2, hnRNPs) and alternative RNA splicing in glioblastoma  SON-mediated gene expression and its role in human neuro-developmental disorders (ZTTK syndrome; OMIM #617140; https://www.omim.org/entry/617140 -- Our study identified the syndrome for the first time)  Characterization and documentation of clinical features of ZTTK syndrome (congenital anomalies, hematopoiesis, telomere regulation and metabolism)  Long-range 3D chromatin interactions in normal and cancer cells  Long non-coding RNAs (MALAT1, GAS5) in hematopoiesis, leukemia and cancer metabolism

Curriculum Vitae, Erin Ahn

Research Support Active R01 CA190688 (PI: Ahn) NIH/NCI 12/01/2015 – 11/30/2020 SON-mediated gene expression and leukemia The goal of this project is to explore the role of the SON protein and its splice variants in controlling epigenetic regulation of transcription associated with leukemogenesis. Total funds $1,893,750 (direct costs: $1,250,000) Percent effort: 25%

UL1TR001417 (PI: Ahn) 07/01/2019 – 04/30/2020 CCST Interdisciplinary Network Pilot Program Grant NIH/CTSA Program (Center for Clinical & Translational Science) through UAB Targeting radiation-resistant glioblastoma by inhibition of SON-mediated RNA splicing The goal of this project is to evaluate the therapeutic potential of blocking SON-mediated RNA regulation in radiation-resistant glioblastoma cells using in vivo mouse models Total funds: $60,000 Percent effort: 15%

USA College of Medicine Intramural Grants Program (PI: Ahn) 03/01/2019 - 2/28/2020 SON-mediated RNA splicing in glioblastoma This is a competitive intramural grant support to promote the success of the R01 application of promising investigators. Total funds: $50,000

Grant with council meeting pending R01 CA236911-01A1 (PI: Ahn) (received 5 percentile) proposed project time: 09/01/2019 – 08/31/2024 NIH/NCI SON-mediated RNA splicing in glioblastoma This project is to achieve a comprehensive understanding of SON function in altering the RNA splicing program in glioblastoma and to evaluate the therapeutic potential of targeting SON in vivo. Total funds requested: $2,271,845. (direct costs: $1,475,225) Percent effort: 25%

Grant in preparation R01 NIH/NHLBI (PI: Ahn) (will be submitted by Feb 5th, 2020) Role of SON in hematopoietic stem cell maintenance and lineage determination This proposal is to study how SON haploinsufficiency and overdose cause hematopoietic stem cell (HSC) fate determination, which contributes to various hematopoietic disorders in human ZTTK syndrome patients and Down syndrome patients. The study will employ newly generated SON knockout mouse models and extensive genomic analyses.

Curriculum Vitae, Erin Ahn Completed R21 CA185818 (PI: Ahn) NIH/NCI 12/02/2014 – 11/30/2017 SON and the MALAT1 locus in chromatin interaction and metastasis gene regulation The goal of this project is to define the role of the MALAT1 genomic locus in chromatin interactions and its cooperation with SON to control metastasis-associated genes in lung cancer using the chromosome conformation capture and sequencing technique.

Publications

1. Kim JH, Stone JK, Li J, Gillespie GY, Flemington EK, Sobol RW, Lim ST, Ahn EE. SON drives oncogenic RNA splicing in glioblastoma by regulating PTBP1/PTBP2 switching and RBFOX2 activity. (under review in Nature Communications)

2. Stone JK, Kim JH, Vukadin L, Richard A, Lim ST, Tan M, Ahn EE. Hypoxia induces cancer cell- specific chromatin interactions and increases MALAT1 expression in breast cancer cells. J Biol Chem (2019) Jul 19; 294(29): 11213-11224

3. Kim JH, Park EY, Chitayat D, Stachura DL, Schaper J, Lindstrom K, Jewett T, Wieczorek D, , Daarisma JM, Sinnema M, Hoeberigs C, Hempel M, Bachman KK, Seeley AH, Shinde DN, Stone, JK, Vukadin L, Richard A, McWalter K, Si YC, Douglas G, Lim ST, Vissers LELM, Lemaire M, Ahn EE. SON haploinsufficiency causes impaired pre-mRNA splicing of CAKUT genes and heterogeneous renal phenotypes. Kidney International (2019) 95 (6):1494-1504.

4. Jeong K, Kim JH, Murphy JM, Park H, Choi C, Schlaepfer DD, Guan JL, Taylor JM, Lincoln T, Ahn EE, Lim ST. Focal adhesion kinase activity and nuclear localization controls vascular smooth muscle cell proliferation and intimal hyperplasia through a GATA4-Cyclin D1 axis. Circ Res. (2019) May 17;doi: 10.1161/CIRCRESAHA.118.314344.

5. Murphy JM, Jeong K, Rodriguez YAR, Kim JH, Ahn EE, Lim ST. FAK and Pyk2 activity promote TNF-α and IL-1β-mediated pro-inflammatory gene expression and vascular inflammation. Sci Rep. (2019) May 20;9(1):7617. doi: 10.1038/s41598-019-44098-2

6. Jeong K, Murphy JM, Rodriguez YAR, Kim JS, Ahn EE, Lim SS.FAK inhibition reduces metastasis of α4 integrin-expressing melanoma to lymph nodes by targeting lymphatic VCAM-1 expression. Biochem. Biophys. Res. Commun. (2019); 509(4):1034-1040

7. Dementiev A, Joachimiak A, Nguyen H, Gorelik A, Illes K, Shabani S, Gelsomino M, Ahn EE, Nagar B, Doan N. Molecular mechanism of inhibition of acid ceramidase by carmofur. J Med Chem (2018) Dec 7. doi: 10.1021/acs.jmedchem.8b01723. [Epub ahead of print]

8. Li J, Svilar D, McClellan S, Kim JH, Ahn EE, Vens C, Wilson DM 3rd, Sobol RW. DNA Repair Molecular Beacon assay: a platform for real-time functional analysis of cellular DNA repair capacity. Oncotarget (2018) 9(60):31719-31743.

9. Doan N, Nguyen HS, Alhajala HS, Jaber B, Al-Gizawiy MM, Ahn E, Mueller WM, Chitambar CR, Mirza SP, Schmainda KM. Identification of radiation responsive genes and transcriptome profiling via complete RNA sequencing in a stable radioresistant U87 glioblastoma model. Oncotarget (2018) 9: 23532-23542.

Curriculum Vitae, Erin Ahn 10. Kim JH, Shinde DN, Reijnders MRF, Hauser NS, Belmonte RL, Wilson GR, Bosch DGM, Bubulya PA, Shashi V, Petrovski S, Stone JK, Park EY, Veltman JA, Sinnema M, Stumpel CTRM, Draaisma JM, Nicolai J, University of Washington Center for Mendelian Genomics, Yntema HG, Lindstrom K, de Vries BBA, Jewett T, Santoro SL, Vogt J, The Deciphering Developmental Disorders Study, Bachman KK, Seeley AH, Krokosky A, Turner C, Rohena L, Hempel M, Kortüm F, Lessel D, Neu A, Strom TM, Wieczorek D, Bramswig N, Laccone FA, Behunova J, Rehder H, Gordon CT, Rio M, Romana S, Tang S, El-Khechen D, Cho MT, McWalter K, Douglas G, Baskin B, Begtrup A, Funari T, Schoch K, Stegmann APA, Stevens SJC, Zhang DE, Traver D, Yao X, MacArthur DG, Brunner HG, Mancini GM, Myers RM, Owen LB, Lim ST, Stachura DL, Vissers LELM, Ahn EYE. De novo loss-of-function mutations in SON disrupt RNA-splicing of genes essential for brain development and metabolism, causing an intellectual disability syndrome. Am J Hum Genet (2016) 99: 711-719

11. Liu Z, Smith KR, Khong HT, Huang J, Ahn E, Zhou M, Tan M. miR-125b regulates differentiation and metabolic reprogramming of T cell acute lymphoblastic leukemia by directly targeting A20. Oncotarget (2016) 7(48):78667-78679

12. Kim JH, Baddoo MC, Park EY, Stone JK, Park H, Butler TW, Huang G, Yan X, Pauli-Behn F, Myers RM, Tan M, Flemington EK, Lim ST, Ahn EY. SON and its alternatively spliced isoforms control MLL complex-mediated H3K4me3 and transcription of leukemia-associated genes. Mol Cell (2016) 61:859-873

13. Schmitt DC, Madeira da Silva L, Zhang W, Liu Z, Arora R, Lim S, Schuler AM, McClellan S, Andrews JF, Kahn AG, Zhou M, Ahn EY, Tan M. ErbB2-intronic microRNA-4728: a novel tumor suppressor and antagonist of oncogenic MAPK signaling. Cell Death Dis (2015) 2015 May; 6(5): e1742.

14. Kim JH, Ahn EY. Transcript variants of RUNX1T1: new insight into t(8;21)-positive leukemia. Leuk Res (2014) 38(9):1018-1019.

15. Hickey CJ, Kim JH, Ahn EY. New discoveries of old SON: A link between RNA splicing and cancer. J Cell Biochem. (2014) 115(2):224-231

16. DeKelver RC, Yan M, Ahn EY, Speck NA, Zhang DE. Attenuation of AML1-ETO cellular dysregulation correlates with increased leukemogenic potential. Blood (2013) 121(18):3714- 3717 17. Desai S, Liu Z, Yao J, Patel N, Chen J, Wu Y, Ahn E, Fodstad O, Tan M. Heat Shock Factor 1 (HSF1) controls chemoresistance and autophagy through transcriptional regulation of Autophagy -related Protein 7 (ATG7). J Biol Chem (2013) 288(13):9165-9176 18. Ahn E, Higashi T, Yan M, Matsuura S, Hickey CJ, Lo MC, Shia WJ, DeKelver RC, Zhang DE. SON regulates GATA-2 through transcriptional control of the miR-23a~27a~24-2 cluster. J Biol Chem (2013) 288(8):5381-5388

19. Lo MC, Peterson LF, Yan M, Cong X, Jin F, Shia WJ, Matsuura S, Ahn, EY, Komeno Y, Ly M, Ommen HB, Hokland P, Willman CL, Ren B, Zhang, DE. Combined gene expression and DNA occupancy profiling identifies potential therapeutic targets of t(8;21) AML. Blood (2012) 120(7):1473-1484

20. Matsuura S., Yan, M., Lo M.C., Ahn EY, Weng S., Dangoor D., Matin M., Higashi T., Feng G.S., and Zhang D.E. Negative Effects of Granulocyte-Macrophage Colony-Stimulating Factor (GM- CSF) Signaling in a Murine Model of t(8;21)-Induced Leukemia. Blood (2012) 119(13): 3155- 3163

Curriculum Vitae, Erin Ahn 21. Ahn EY, DeKelver RC, Lo MC, Nguyen TA, Matsuura S., Boyapati A., Pandit S., Fu X.D., and Zhang, D.E. SON controls cell cycle progression by coordinated regulation of RNA splicing. Mol Cell (2011) 42: 185-198

22. Pawar P., Ma L., Byon C.H., Liu H., Ahn EY, Jhala N., Arnoletti J.P., McDonald J.M. and Chen Y. Molecular mechanisms of tamoxifen therapy for cholangiocarcinoma: Role of calmodulin. Clin Cancer Res (2009) 15(4):1288-1296

23. Ahn EY, Yan M., Malakhova O.A., Lo M.C., Boyapati A., Ommen H.B., Hines R., Hokland P., and Zhang D.E. Disruption of the NHR4 domain structure in AML1-ETO abrogates SON binding and promotes leukemogenesis. Proc Natl Acad Sci U S A (2008) 105(44): 17103- 17108.

24. Yan M., Ahn EY, Hiebert S., Zhang D.E. RUNX1/AML1 DNA binding domain and ETO/MTG8 NHR2 oligomerization domain are critical to AML1-ETO9a induced leukemogenesis. Blood (2008) 113: 883-886

25. Dayyani F., Wang J., Yeh JR, Ahn EY, Tobey E., Zhang Y., Zaidi S.T., Zhang D.E., Bernstein, I.D., Peterson, R.T., and Sweetser, D.A. Loss of TLE1 and TLE4 from the del(9q) commonly deleted region in AML cooperate with AML1-ETO to affect myeloid cell proliferation and survival. Blood (2008) 111 (8); 4338-4347.

26. Peterson LF, Boyapati A., Ahn EY, Biggs JR., Okumura A., Lo M.C., Yan M., and Zhang D.E. Acute Myeloid Leukemia with the 8q22;21q22 Translocation: Secondary Mutational Events and Alternative t(8;21) Transcript. Blood (2007) 110: 799-805.

27. Pan G., Ahn EY, Chen Y., Feng G., Reddy V., Jhala N.C., and McDonald J.M. (2007) Reciprocal co-expression of Fas and Fas ligand in human cholangiocarcinoma. Int J Oncol (2007) 31(4):843-50.

28. Wu X., Ahn EY, McKenna M.A., Yeo H, and McDonald J.M. Fas binding to calmodulin regulates apoptosis in osteoclasts. J Biol Chem (2005) 280: 29964-29970

29. Ahn EY, Lim ST, Cook WJ, and McDonald JM. Calmodulin binding to the Fas death domain: Regulation by Fas activation. J Biol chem (2004) 279: 5661-5666

30. Ahn EY, Pan,G, Oh, JH, Tytler EM, and McDonald JM. The combination of calmodulin antagonists and interferon-gamma induces apoptosis through caspase-dependent and – independent pathways in cholangiocarcinoma cells. Am J Pathol (2003) 163: 2053-2063

31. Ahn EY, Pan G, Vickers SM, and McDonald JM. Interferon-gamma upregulates apoptosis- related moluecules and enhances Fas-mediated apoptosis in human cholangiocarcinoma. Int J Cancer (2002) 100: 445-451

32. Vickers SM, Ahn EY, Jhala NC, Pan G, Bland KI, and McDonald JM. (2002) Tamoxifen/Fas- induced growth inhibition of human cholangiocarcinoma by gamma-interferon. Ann Surg (2002) 235: 872-878

33. Lee JE, Ahn, EY, Ahn TI. Development of a plasmid vector for overproduction of beta- galactosidase in E. coli by using genetic components of groEx from symbiotic bacteria in Amoeba proteus. Microbiol Biotechnol (1998) 8: 509-516

Curriculum Vitae, Erin Ahn

Published Abstracts

1. Kim JH, Vukadin L, Hackwelder TB, Ungerleider N, Flemington EK, Ahn E. Hematological disorders in human patients with SON mutations. FASEB Summer Research Conference: Hematological Malignancies. July 28-Aug 2 (2019), Snowmass Village, CO.

2. Kim JH, Stone JK, Li J, Richard A, Vukadin L, Gillespie GY, Sobol RW, Lim ST, Ahn EYE. SON controls the oncogenic alternative splicing program in glioblastoma by regulating PTBP1/2 switch and RBFOX2 activity. Proceedings of the Annual Meeting of the American Association for Cancer Research (2018) Apr 14-18; Chicago, IL. (Late-Breaking Research Abstract)

3. Stone JK, Kim JH, Tan M, Ahn EYE. Hypoxia upregulates the long noncoding RNA MALAT1 through increased chromatin looping. Proceedings of the Annual Meeting of the American Association for Cancer Research (2018) Apr 14-18; Chicago, IL. 4. Vukadin L, Park EY, Kim JH, Ahn EYE. SON represses RUNX1 expression and impairs megakaryocytic differentiation in Down syndrome acute megakaryoblastic leukemia (DS-AMKL). Proceedings of the Annual Meeting of the American Association for Cancer Research (2018) Apr 14-18; Chicago, IL. 5. Richard A, Abdel-Rahman JH, Kim JH, Vukadin L, Piazza GA, Ahn EYE. A novel quinazolin- 4(3H)-one/Schiff base hybrid phosphodiesterase 4 inhibitor as a potential therapeutic agent for leukemia. Proceedings of the Annual Meeting of the American Association for Cancer Research (2018) Apr 14-18; Chicago, IL. 6. Stone JK, Kim JH, Tan M, Ahn EYE. Hypoxia upregulates the long noncoding RNA MALAT1 through increased chromatin looping. Keystone Symposia: Noncoding RNAs: Form, Function, Physiology (2018) Feb 25-Mar 1; Keystone, CO. 7. Dinerman A, Kim JH, Ahn EYE, Butler T. Investigation of the Significance of SON Isoform mRNA Expression in Patients with Blood Cytopenias with/without Evidence of Myeloid Neoplasia American Society of Hematology Annual Meeting (2017)

8. Lim J, Kim JH, Ahn EYE. Combinational Treatment of Imatinib and Allicin Synergistically Inhibits the Growth of BCR-ABL-Positive Leukemia and the Emergence of Drug-Resistant Cells. American Society of Hematology Annual Meeting (2017)

9. Stone, J.K., Kim, J.H., Tan, M., Ahn, EYE. Hypoxia upregulates the long noncoding RNA MALAT1 in breast cancer through increased chromatin looping. UAB Comprehensive Cancer Center Annual Retreat, October 2017.

10. Vukadin L, Park EY, Ahn EYE. SON represses RUNX1 expression and impairs megakaryocytic differentiation in Down syndrome acute megakaryoblastic leukemia (DS-AMKL). UAB Comprehensive Cancer Center Annual Retreat, October 2017.

11. Richard A, Kim JH, Vukadin L, Abdel-Rahman HM, Piazza GA, Ahn EYE. A novel quinazolin- 4(3H)-one/schiff base hybrid phosphodiesterase 4 inhibitor induces growth arrest, DNA Double- stranded-break and differentiation in acute megakaryoblastic leukemia cells. UAB Comprehensive Cancer Center Annual Retreat, October 2017.

12. Kim JH, Stone JK, Li J, Richard A, Vukadin L, Gillespie GY, Sobol RW, Lim S, Ahn EYE. SON controls the oncogenic alternative splicing program in glioblastoma by regulating PTBP1/2 switch and RBFOX2 activity. UAB Comprehensive Cancer Center Annual Retreat, October 2017.

Curriculum Vitae, Erin Ahn 13. Kim JH, Ahn EYE. Regulation of leukemogenic potential by a splice variant of SON in MLL- rearranged leukemia. FASEB Science Research Conferences: Hematologic Malignancies (Saxtons River, VN. July 2017)

14. Kim JH, Shinde DN, Reijnders MRF, Hauser NS, Belmonte RL, Wilson GR, Bosch DGM, Bubulya PA, Shashi V, Petrovski S, Stone JK, Park EY, Veltman JA, Sinnema M, Stumpel CTRM, Draaisma JM, Nicolai J, University of Washington Center for Mendelian Genomics, Yntema HG, Lindstrom K, de Vries BBA, Jewett T, Santoro SL, Vogt J, The Deciphering Developmental Disorders Study, Bachman KK, Seeley AH, Krokosky A, Turner C, Rohena L, Hempel M, Kortüm F, Lessel D, Neu A, Strom TM, Wieczorek D, Bramswig N, Laccone FA, Behunova J, Rehder H, Gordon CT, Rio M, Romana S, Tang S, El-Khechen D, Cho MT, McWalter K, Douglas G, Baskin B, Begtrup A, Funari T, Schoch K, Stegmann APA, Stevens SJC, Zhang DE, Traver D, Yao X, MacArthur DG, Brunner HG, Mancini GM, Myers RM, Owen LB, Lim ST, Stachura DL, Vissers LE, Ahn EYE. De novo loss-of-function mutations in SON disrupt RNA-splicing of genes essential for brain development and metabolism, causing an intellectual disability syndrome. American Society of Human Genetics Annual Meeting (selected as a top scored abstract for Platform Presentation) (Vancouver, B.C., October, 2016)

15. Kim JH, Dorset D, Myers R, Ahn E. Analysis of long-range gene regulation by the lncRNA MALAT1 genomic locus using enhanced chromosome conformation capture & sequencing (e4C) in lung cancer cells. American Society of Human Genetics Annual Meeting (Vancouver, B.C, October 2016)

16. Kim JH, Park H, Kong H, Murphy JM, Schlaepfer DD, Ahn E, Lim S. Role of focal adhesion kinase signaling in vascular smooth muscle cell migration, proliferation,and intimal hyperplasia. American Society for Cell Biology Annual Meeting (November, 2016) Abstract published as: J. Kim, H. Park, H. Kong, J.M. Murphy, D.D. Schlaepfer, E. Ahn, S. Lim. (2016) Role of focal adhesion kinase signaling in vascular smooth muscle cell migration, proliferation,and intimal hyperplasia. Mol. Biol. Cell 26, 4523 (657).

17. Murphy JM, Kim JH, Ahn E, Lim S. Focal adhesion kinase promotes pro‐inflammatory molecule expression and atherosclerosis via NF-kB activation. NF‐κB activation. American Society for Cell Biology Annual Meeting (November, 2016) Abstract published as: J.M. Murphy, J. Kim, E. Ahn, S. Lim. (2016) Focal adhesion kinase promotes pro‐inflammatory molecule expression and atherosclerosis via NF‐κB activation. Mol. Biol. Cell 26, 4523 (1404).

18. Kim JH, Park EY, Stone JK, Butler TW, Ahn EY. SON and its splice variants regulate MLL1/2 complex-mediated H3K4me3 and transcription of leukemia-associated genes. Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA: Cancer Research 76(14 Supplement):1978. Abstract no. 1978.

19. Stone JK, Kim JH, Tan M, Ahn EY. NFATC1 regulates the long non-coding RNA MALAT1 in breast cancer. Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA: AACR; Cancer Res. 2016;76(14 Suppl):1835. Abstract no.1835

20. Phillips J, Kim JH, Lim S, Ahn EY, Tan M. Regulation of RNA splicing of the ErbB family receptors by the splicing cofactor SON. Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA: AACR; Cancer Res 2016;76(14 Supplement):1996. Abstract no. 1996.

Curriculum Vitae, Erin Ahn 21. Kim JH, Butler TW, Ahn EY. SON and its alternatively spliced isoforms control MLL complex- mediated H3K4me3 and transcription of leukemia-associated genes. American Society of Hematology 57th Annual Meeting (2015)

Abstract published as: Kim JH, Butler TW, Ahn EY. SON and its alternatively spliced isoforms control MLL complex-mediated H3K4me3 and transcription of leukemia-associated genes. Blood 2015 126:2426

22. Kim JH, Hickey CJ, Ahn EY. Aberrant Upregulation of Alternatively Spliced Isoforms of SON in Myeloid Leukemia: Implications in Hematopoiesis and Leukemogenesis. American Society of Hematology 55th Annual Meeting (2013)

23. Ahn E, Higashi T, Yan M, Matsuura S, Hickey CJ, Lo MC, Shia WJ, DeKelver RC, Zhang DE. SON regulates GATA-2 through transcriptional control of the miR-23a~27a~24-2 cluster. American Society of Hematology 54th Annual Meeting (2012)

24. Ahn EY, DeKelver RC, Lo MC, Zhang DE. SON, a novel AML1-ETO-interacting protein, is critical for RNA splicing of the genes involved in genomes stability and cell cycle progression. The 17th International RUNX workshop (2010)

25. DeKelver RC, Yan M, Ahn EY, Speck NA, Zhang DE. Relative importance of NCoR and SON interactions with NHR4 domain of AML1-ETO in leukemogenesis. The 17th International RUNX workshop (2010)

26. Yao X, Ahn EY, Stachura D, Zhang DE. SON DNA-binding protein homologue is critical in zebrafish embryo development. UCSD Biological Sciences Student Research Showcase (2010)

27. Matsuura S, Yan M, Ahn EY, Lo MC, Dangoor D, Matin M, Burgoyne P, Zhang DE. Negative effect of GM-CSF signaling in t(8;21)-induced leukemia. American Society of Hematology 52th Annual Meeting (2010)

28. Ahn EY, DeKelver RC, Yan M, Zahgn DE. Alteration of SON localization in t(8;21)-assocaited acute myeloid leukemia. FASEB Summer Research Conferences: Hematological Malignancies (2009)

29. Ahn EY, Yan M, Malakhova OA, Lo MC., Boyapati A, Ommen HB, Hines R, Hokland P, Zhang DE.Disruption of the NHR4 domain structure in AML1-ETO abrogates SON binding and promotes leukemogenesis. American Society of Hematology 50th Annual Meeting (2008)

30. Ahn EY, Yan M, Boyapati A, Hines R, Zhang DE. Disruption of the MYND zinc finger domain of AML1-ETO abrogates its interaction with SON and promotes leukemogenesis. Mechanisms and Models of Cancer, No.2 (2007) (abstract #77)

31. Ahn EY, Pan G, Oh JH, McDonald JM. The combination of calmodulin antagonists and interferon-gamma induces apoptosis through caspase-dependent and –independent pathways in cholangiocarcinoma cells. Proceedings of the 94th Annual Meeting of the American Association for Cancer Research (2003) 44, 2nd Ed ; 612 (#R3088)

32. Ahn EY, Pan G, Vickers SM, McDonald JM. Interferon-gamma enhances calmodulin antagonist- mediated apoptosis in human cholangiocarcinoma. Proceedings of the AACR special conference, Apoptosis and Cancer: Basic Mechanisms and Therapeutic opportunities in the Post-Genomic Era (2002) (# A-2)

Curriculum Vitae, Erin Ahn 33. Ahn EY, Pan G, McDonald JM. Interferon-gamma enhances calmodulin antagonist-mediated cytochrome c release and apoptosis in human cholangiocarcinoma. Proceedings of the 92nd Annual Meeting of the American Association for Cancer Research (2001) 42; 96 (#518)

34. Ahn EY, Pan G, Pickens A, McDonald JM. Interferon-gamma increases Fas expression and apoptosis in human cholangiocarcinoma. Proceedings of the 91st Annual Meeting of the American Association for Cancer Research (2000) 41; 528 (#3364)

35. Ahn EY, Lee JE, and Ahn TI. Development of a plasmid vector for overproduction of beta- galactosidase in E. coli by using genetic components of groEx from symbiotic bacteria in Amoeba proteus. Korean Society of Molecular Biology and Biochemistry (1997)

Presentations

Invited speaker, Splicing Factor Mutations and RNA Biology in Cancer Workshop, Yale University Cancer Center (May 2019) Invited Speaker, Stanley S. Scott Cancer Center Invited Speaker Series, LSU Health New Orleans, School of Medicine (Jul 2018) Invited Speaker, Herman B Wells Center for Research Seminar Series, Department of Pediatrics, Indiana University School of Medicine (Feb 2018) Invited Speaker, Department of Pediatrics, Division of Hematology & Oncology, The University of Oklahoma Health Sciences Center (Sept 2017) Invited Speaker, Special Seminar, Division of Hematology & Oncology, UAB School of Medicine (July 2017) Invited Speaker, Hematology & Oncology Research Conference, UAB School of Medicine (August 2016) Invited Speaker, Cell and Molecular Biology Seminar Series, College of Medicine, University of South Alabama (April 2016) Invited Speaker, Workshop on Myeloid development, American Society of Hematology Annual meeting, Orlando, FL (Dec 2015) Invited Speaker, Workshop on Myeloid development, American Society of Hematology Annual meeting, New Orleans, LA (Dec 2013) Seminar in Department of Biochemistry and Molecular Biology, College of Medicine, University of South Alabama (Mar 2013) Invited Speaker, Cell Biology Seminar Series, College of Medicine, University of South Alabama (Feb 2013) Oral presentation, American Society of Hematology Annual meeting, Atlanta, GA (Dec 2012)

Invited Speaker, Seminar Series at HudsonAlpha Institute for Biotechnology, Huntsville, AL (Nov 2012) Invited Speaker, Korean Women in Science and Engineering (KWiSE) 6th Annual West Coast Symposium (May 2012) Seminar in Department of Biochemistry and Molecular Biology, USA College of Medicine (Nov 2011) Seminar in Mitchell Cancer Institute, University of South Alabama. (Sept 2011) 11

Curriculum Vitae, Erin Ahn

Invited Speaker, The 17th International RUNX Workshop, Hiroshima, Japan (Jul 2010) Invited Speaker, Pathology Research Lecture Series, Department of Pathology, University of California San Diego (Jun 2010) Invited Speaker, Basic and Translation Research Round, Moores Cancer Center, University of California San Diego (May 2008) Invited Speaker, Korean Women in Science and Engineering (KWiSE) Research Forum (Oct 2007)

Patent Applications (filed)

1. Ahn E, Lim S, Kim JH. U.S. Patent application entitled “Methods and compositions for the diagnosis of acute myeloid leukemia” has been filed in December 2016. (PCT/US2016/064442) 2. Ahn E, Lim S, Kim JH. U.S. Patent application entitled “Methods and compositions for detecting neurodevelopmental disorders” has been filed in December 2016. (PCT/US2016/065669)

Teaching and Mentoring Experience a. Class Teaching:

Graduate course IDL-560 Cancer Biology: Cancer Cell Cycle, Cancer Stem Cells and Epigenetics (Spring, 2014, Spring 2015, Spring 2017)

Graduate course IDL- 580 Fundamentals in Basic Medical Sciences II (Hematopoiesis section, Spring 2015, Spring 2016, Spring 2017, Spring 2018, Spring 2019)

Graduate course IDL- 580 Fundamentals in Basic Medical Sciences II (RNA splicing, Genetic basis of cancer, Fall 2018)

b. Graduate Students Directly Supervised Manal Al Sheikh (USA Environmental Toxicology MS Degree Program, 2014- 2015) Joshua Stone (Ph.D. inn BMS Graduate Program, 2014 - March 2018; current position – Postdoctoral fellow at NIH/NCI) Lana Vukadin (Ph.D student in BMS Graduate Program, June 2016-present) Alexander Richard (Ph.D student in BMS Graduate Program, June 2016-present) Co-mentor of Joshua Phillips (Ph.D students in BMS Graduate Program, major mentor: Dr. Ming Tan, 2014-2018) Co-mentor of Kelly Smith (Ph.D. students in BMS Graduate Program, major mentor: Dr. Ming Tan, 2015-2016) c. BMS Graduate Student Dissertation Committee Sara Sigler (2012-2015) Courey Averett (2013 – 2016) James Murphy (2015-2018) Reagan Barnett (2015) Ahmed Alghadhban (2017)

Curriculum Vitae, Erin Ahn Jensyn Seiger (2017-present) d. Mentoring Rotation Students Courey Averett (Spring 2013) Joe Otto (Spring 2013) Orlandric Miree (Spring 2016) Jenny Hewes (Fall 2017) e. Postdoctoral Fellows Supervised Current Postdoctoral trainees: Jung-Hyun Kim (Feb 2013-present) Past postdoctoral trainees: Christopher Hickey (2012-2013) Wonyoung Kim (2013-2014) Eun Young Park (Apr 2015-Oct 2017) Hyunkyung Kong (Jun 2016-Nov 2017) f. Medical Student Supervised Aaron Dinerman (May 2017 - present) Brittaney Jackson (Summer 2019) g. Undergraduate Students Supervised Kristina Villafria (Oct 2012 - Jun 2013) Erika Tumulak (Jul 2013 – Dec 2013) Lawrence Pierce (Jun 2015 – Jan 2016) William Brannan (Jun 2016 –Aug 2016) Hannah Giannini (Summer 2017, Fall 2018 - present) Johnny Tran (Jan 2019 – present) h. High School Student Supervised Bronson Wessinger (Jun 2013 – Jul 2013) Jamie Lim (Fall 2015, Fall 2016) i. Honors/Awards won by Trainees Jung-Hyun Kim (2013): Abstract Achievement Award (American Society of Hematology Annual Meeting, New Orleans, LA) Jung-Hyun Kim (2015): Abstract Achievement Award (American Society of Hematology Annual Meeting, Orlando, FL)

Curriculum Vitae, Erin Ahn Jung-Hyun Kim (2016-2017): Lady Tata Memorial Trust International Award (Postdoctoral Fellowship) Jamie Lim (2016, 2017): 1st Place, Cell and Molecular Biology Category, Alabama Science and Engineering Fair, Finalist at the International Science and Engineering Fair. Jung-Hyun Kim (2017): The FASEB Hematologic Malignancies Conference Poster Award

Alexander Richard (2017) Best Research Presentation Award. College of Medicine Annual Research Forum

Joshua Stone (2018) The Samuel J. Strada Travel and enhancement Award. BMS Graduate Program. College of Medicine, University of South Alabama.