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1 Curriculum Vitae Curriculum Vitae, Erin Ahn Curriculum Vitae Full name: Eun-Young Erin Ahn, Ph.D. Current Position Associate Professor Department of Biochemistry and Molecular Biology Mitchell Cancer Institute College of Medicine University of South Alabama Campus Address Permanent Address 1660 Springhill Ave. 1425 Oak Vale Ct. Mobile, AL 36604 Mobile, AL 36609 Telephone (Work) 1-251-445-9805 E-mail: [email protected] Education & Training BS (1992-1996) Seoul National University Department of Biology Education Seoul, Korea MS (1996-1998) Seoul National University Department of Biology Education Seoul, Korea Thesis : Development of an overexpression system for beta-galactosidase in Escherichia coli using genetic elements of groEx. Ph.D. (1998-2003) University of Alabama at Birmingham Department of Pathology Molecular & Cellular Pathology Graduate Program Birmingham, AL (Advisor: Jay M. McDonald, M.D.) Thesis: Molecular mechanisms of apoptosis in human cholangiocarcinoma: Regulation by Fas, calcium/calmodulin and interferon-gamma. Postdoctoral Fellow (2004 - June 2009) Scripps Research Institute (2004 – Feb 2008) Department of Molecular and Experimental Medicine La Jolla, CA (Advisor : Dong-Er Zhang, Ph.D.) University of California San Diego (Mar 2008 – Jun 2009) Moores UCSD Cancer Center La Jolla, CA (Advisor: Dong-Er Zhang, Ph.D.) Assistant Project Scientist (July 2009 – June 2012) University of California San Diego Moores UCSD Cancer Center La Jolla, CA 1 Curriculum Vitae, Erin Ahn Professional Positions and Experience Assistant Professor (July 2012 – July 2016) Division of Oncologic Science Mitchell Cancer Institute University of South Alabama, Mobile, AL Assistant Professor (July 2012 – July 2016) Division of Oncologic Science Mitchell Cancer Institute University of South Alabama, Mobile, AL Associate Professor (with tenure; August 2016 – present) Division of Oncologic Science Mitchell Cancer Institute University of South Alabama, Mobile, AL Associate Professor (May 2019 - present) Department of Biochemistry and Molecular Biology College of Medicine University of South Alabama, Mobile, AL Awards and Honors RARE Champion of Hope Award Nominee, Global Genes (2019) https://globalgenes.org/rare-champion-of-hope-award-nominees/ Human Diseases Genes Website Series – Moderator for the SON gene (2019-present) https://humandiseasegenes.nl/son/ https://humandiseasegenes.nl/moderators/ Highlighted in News “MCI teams finds link to rare disorder”, (2017) http://www.checkorphan.org/news/mci-team-finds-link-to-rare-disorder The Mayer Mitchell Award for Excellence in Cancer Research (2017) Travel Award. 50th annual Meeting, American Society of Hematology (2008) International Awards for Research in Leukemia and Allied Conditions, Lady TATA Memorial Trust, United Kingdom. Postdoctoral fellowship (2005-2007) Best Research Presentation Award. Moores UCSD Cancer Center Annual Retreat (2007) The John R. Durant Award for the Excellence in Cancer Research. First Place in Graduate Student Category. Comprehensive Cancer Center, University of Alabama at Birmingham (2001) College Dean Award (1st honor) for the excellence in undergraduate study. Seoul National University, Seoul, Korea (1996) Membership Member, American Association for Cancer Research Member, American Society of Hematology Member, American Society of Human Genetics 2 Curriculum Vitae, Erin Ahn Academic Service Reviewer, NIH CSR Study Sections NIH CAMP Study Section (February 2017) NIH CAMP Study Section (June 2017) NIH MONC Study Section (October 2017) NIH/NCI Special Emphasis Panel ZCA1 RPRB-F (O1) for P01 review (June 2017) NIH Special Emphasis Panel/Scientific Review Group ZRG1 F09B-M (20) (November 2018) NIH Cancer Genetic (CG) Study Section (June 2019) NIH Special Emphasis Panel/Scientific Review Group ZRG1 F09B-M (20) L (July 2019) NIH Cancer Genetic (CG) Study Section (October 2019) Other academic service Member, Mitchell Cancer Institute Biosafety Committee Member, College of Medicine Flow Cytometry Facility Advisory Committee Editorial Board, Journal of Cancer Research and Therapeutic Oncology, Editorial Board, Journal of Cancer Diagnosis Reviewer, Abraham Mitchell Cancer Research Award Grant (2013) Ad hoc Reviewer for journals: Cell Reports, Blood, Leukemia Research, Leukemia and Lymphoma, PLoS One, Molecular and Cellular Biology, British Journal of Cancer, International Journal of Molecular Sciences, Journal of cancer Diagnosis, Journal of Cancer Sciences. Organizing Assistant for The18th International RUNX Workshop, San Diego, CA (2011) Community service and other extramural activities Touch-A-Truck Pediatric Cancer Research fund-raising event committee (2017- present) Mitchell Cancer Institute representative. Women in STEM Teen Conference and Career Expo, Alabama School of Math and Science, AL (2017) Research Interests SON-mediated RNA splicing and epigenetic regulation of transcription in normal hematopoiesis and leukemia RNA-binding proteins (SON, RBFOX2, hnRNPs) and alternative RNA splicing in glioblastoma SON-mediated gene expression and its role in human neuro-developmental disorders (ZTTK syndrome; OMIM #617140; https://www.omim.org/entry/617140 -- Our study identified the syndrome for the first time) Characterization and documentation of clinical features of ZTTK syndrome (congenital anomalies, hematopoiesis, telomere regulation and metabolism) Long-range 3D chromatin interactions in normal and cancer cells Long non-coding RNAs (MALAT1, GAS5) in hematopoiesis, leukemia and cancer metabolism 3 Curriculum Vitae, Erin Ahn Research Support Active R01 CA190688 (PI: Ahn) NIH/NCI 12/01/2015 – 11/30/2020 SON-mediated gene expression and leukemia The goal of this project is to explore the role of the SON protein and its splice variants in controlling epigenetic regulation of transcription associated with leukemogenesis. Total funds $1,893,750 (direct costs: $1,250,000) Percent effort: 25% UL1TR001417 (PI: Ahn) 07/01/2019 – 04/30/2020 CCST Interdisciplinary Network Pilot Program Grant NIH/CTSA Program (Center for Clinical & Translational Science) through UAB Targeting radiation-resistant glioblastoma by inhibition of SON-mediated RNA splicing The goal of this project is to evaluate the therapeutic potential of blocking SON-mediated RNA regulation in radiation-resistant glioblastoma cells using in vivo mouse models Total funds: $60,000 Percent effort: 15% USA College of Medicine Intramural Grants Program (PI: Ahn) 03/01/2019 - 2/28/2020 SON-mediated RNA splicing in glioblastoma This is a competitive intramural grant support to promote the success of the R01 application of promising investigators. Total funds: $50,000 Grant with council meeting pending R01 CA236911-01A1 (PI: Ahn) (received 5 percentile) proposed project time: 09/01/2019 – 08/31/2024 NIH/NCI SON-mediated RNA splicing in glioblastoma This project is to achieve a comprehensive understanding of SON function in altering the RNA splicing program in glioblastoma and to evaluate the therapeutic potential of targeting SON in vivo. Total funds requested: $2,271,845. (direct costs: $1,475,225) Percent effort: 25% Grant in preparation R01 NIH/NHLBI (PI: Ahn) (will be submitted by Feb 5th, 2020) Role of SON in hematopoietic stem cell maintenance and lineage determination This proposal is to study how SON haploinsufficiency and overdose cause hematopoietic stem cell (HSC) fate determination, which contributes to various hematopoietic disorders in human ZTTK syndrome patients and Down syndrome patients. The study will employ newly generated SON knockout mouse models and extensive genomic analyses. 4 Curriculum Vitae, Erin Ahn Completed R21 CA185818 (PI: Ahn) NIH/NCI 12/02/2014 – 11/30/2017 SON and the MALAT1 locus in chromatin interaction and metastasis gene regulation The goal of this project is to define the role of the MALAT1 genomic locus in chromatin interactions and its cooperation with SON to control metastasis-associated genes in lung cancer using the chromosome conformation capture and sequencing technique. Publications 1. Kim JH, Stone JK, Li J, Gillespie GY, Flemington EK, Sobol RW, Lim ST, Ahn EE. SON drives oncogenic RNA splicing in glioblastoma by regulating PTBP1/PTBP2 switching and RBFOX2 activity. (under review in Nature Communications) 2. Stone JK, Kim JH, Vukadin L, Richard A, Lim ST, Tan M, Ahn EE. Hypoxia induces cancer cell- specific chromatin interactions and increases MALAT1 expression in breast cancer cells. J Biol Chem (2019) Jul 19; 294(29): 11213-11224 3. Kim JH, Park EY, Chitayat D, Stachura DL, Schaper J, Lindstrom K, Jewett T, Wieczorek D, , Daarisma JM, Sinnema M, Hoeberigs C, Hempel M, Bachman KK, Seeley AH, Shinde DN, Stone, JK, Vukadin L, Richard A, McWalter K, Si YC, Douglas G, Lim ST, Vissers LELM, Lemaire M, Ahn EE. SON haploinsufficiency causes impaired pre-mRNA splicing of CAKUT genes and heterogeneous renal phenotypes. Kidney International (2019) 95 (6):1494-1504. 4. Jeong K, Kim JH, Murphy JM, Park H, Choi C, Schlaepfer DD, Guan JL, Taylor JM, Lincoln T, Ahn EE, Lim ST. Focal adhesion kinase activity and nuclear localization controls vascular smooth muscle cell proliferation and intimal hyperplasia through a GATA4-Cyclin D1 axis. Circ Res. (2019) May 17;doi: 10.1161/CIRCRESAHA.118.314344. 5. Murphy JM, Jeong K, Rodriguez YAR, Kim JH, Ahn EE, Lim ST. FAK and Pyk2 activity promote TNF-α and IL-1β-mediated pro-inflammatory gene expression and vascular inflammation. Sci Rep. (2019) May 20;9(1):7617. doi: 10.1038/s41598-019-44098-2 6. Jeong K, Murphy JM,
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