BIODIVERSITAS ISSN: 1412-033X Volume 22, Number 6, June 2021 E-ISSN: 2085-4722 Pages: 3326-3332 DOI: 10.13057/biodiv/d220638 Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia ELVY LIKE GINTING1,♥, LETHA L. WANTANIA2, EMMA MAUREN MOKO3, REINY A. TUMBOL1, MAYSE S. SIBY1, STENLY WULLUR1 1Faculty of Fisheries and Marine Science, Universitas Sam Ratulangi. Jl. Kampus Unsrat, Manado 95115, North Sulawesi, Indonesia. Tel./fax.: +62-431-868027, email:
[email protected] 2Faculty of Mathematics and Natural Sciences, Bonn University. D-53012 Bonn, Germany 3Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Manado. Jl. Raya Tondano, Minahasa 95618, North Sulawesi, Indonesia Manuscript received: 1 April 2021. Revision accepted: 24 May 2021. Abstract. Ginting EL, Wantania LL, Moko EM, Tumbol RA, Siby MS, Wulur S. 2021. Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. Biodiversitas 22: 3326-3332. The aims of the research were to isolate and identify the amylase-producing thermophilic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. The bacteria were characterized based on the colony and cell morphology and subsequently screened for their amylase activities. The bacterial isolates were identified based on 16S rRNA gene sequences. There were 12 thermophilic bacteria isolates from Likupang Marine Hydrothermal that were able to produce amylase. Two selected isolates (L3 and L9) had an amylolytic index value in the range of 3.04-3.52 at 55oC. The colonies of L3 and L9 are circular, and they are Gram positive, rod-shaped, and motile bacteria.