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(12) Patent Application Publication (10) Pub. No.: US 2008/0311640 A1 Cox Et Al

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(12) Patent Application Publication (10) Pub. No.: US 2008/0311640 A1 Cox Et Al US 20080311640A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2008/0311640 A1 Cox et al. (43) Pub. Date: Dec. 18, 2008 (54) ANAEROBC PRODUCTION OF HYDROGEN Related U.S. Application Data AND OTHER CHEMICAL PRODUCTS (60) Provisional application No. 60/678,101, filed on May 3, 2005, provisional application No. 60/677,856, filed (76) Inventors: Marion E. Cox, Morgan Hill, CA on May 3, 2005, provisional application No. 60/678, (US); Jeremy N. McDonald, San 077, filed on May 3, 2005, provisional application No. 60/678,100, filed on May 3, 2005, provisional appli Jose, CA (US); Laura M. Nondorf, cation No. 60/678,098, filed on May 3, 2005, provi Morgan Hill, CA (US); Steven M. sional application No. 60/677,998, filed on May 3, Cox, Morgan Hill, CA (US) 2005. Publication Classification Correspondence Address: WILSON SONSIN GOODRCH & ROSAT (51) Int. C. 650 PAGE MILL ROAD CI2P3/00 (2006.01) CI2M 3/00 (2006.01) PALO ALTO, CA 94304-1050 (US) CI2M I/36 (2006.01) CI2N L/20 (2006.01) (21) Appl. No.: 11/912,881 (52) U.S. Cl. ................... 435/168; 435/290.4; 435/286.1: 435/303.2:435/252.1 (22) PCT Fled: Apr. 27, 2006 (57) ABSTRACT Described herein are methods for producing chemical prod (86) PCT NO.: PCT/USO6/16332 ucts by anaerobically fermenting a particular biomass using anaerobic bacteria. Such chemical products includehydrogen S371 (c)(1), and other gases, acetic acid and other volatile organic acids, (2), (4) Date: Jun. 23, 2008 Solvents, Solids, and salts of Volatile organic acids. BOMASS Gas Separation Cr- Use of Chemical Gne)NH gas Products Sterilization Deoxygenation Solvent purification Concentration Proteolytic Bacteria Digester for Dark Fermentation Wolatile Filtration Organic Acids Volatile Organic Acid SepaEation and Palification Use of Chemical Or Products Conversion to Salt Of Wolatile Organic Acid Patent Application Publication Dec. 18, 2008 Sheet 1 of 18 US 2008/0311640 A1 “Hfiu||O??uOW3InSS313'Hd eln?eledujeLpue |9.Infi?H Patent Application Publication Dec. 18 , 2008 Sheet 2 of 18 US 2008/0311640 A1 + z?un61-I ?Iqe??BAW SSeuJ01E Patent Application Publication Dec. 18, 2008 Sheet 3 of 18 US 2008/0311640 A1 €3.Infi!-- gaeae,§. Patent Application Publication Dec. 18, 2008 Sheet 4 of 18 US 2008/0311640 A1 Patent Application Publication Dec. 18, 2008 Sheet 5 of 18 US 2008/0311640 A1 ?un61-IG ws,?---- Patent Application Publication Dec. 18, 2008 Sheet 6 of 18 US 2008/0311640 A1 9?un61-I ?sg???gg| #####***** Patent Application Publication Dec. 18, 2008 Sheet 7 of 18 US 2008/0311640 A1 NISSEROO(le?5) NOLLVLS801 NOLLVISZ?.1 SISATIVNV ?70] SISÄTVNV/èHELTld|NOSO LVLSYRIONANOI.Z01 Infil3J SO?LO&ORI ESOLAECI 0!...I Å5OOTO18|| 001 Patent Application Publication Dec. 18, 2008 Sheet 8 of 18 US 2008/0311640 A1 8,8±OV–RIELNI XIÈHONALEN 808CISVOI 8aun61-I ff08ESTOW 908AVTdSICH Cl}}\/OEAEX 008 Patent Application Publication Dec. 18, 2008 Sheet 9 of 18 US 2008/0311640 A1 Patent Application Publication Dec. 18, 2008 Sheet 10 of 18 US 2008/0311640 A1 .*** .*** ...• .-* ...? • •? ...? …• SSBUu0?g?Jedeu) º?eue6ÁXoaq---------sueuisnípyUO?e??u00uOOSSBUUO?g Patent Application Publication Dec. 18, 2008 Sheet 11 of 18 US 2008/0311640 A1 ??eun61-I Patent Application Publication Dec. 18, 2008 Sheet 12 of 18 US 2008/0311640 A1 Z),ºun61-I Patent Application Publication Dec. 18, 2008 Sheet 13 of 18 US 2008/0311640 A1 £),ºun61-I Sseuo?g?OUO?e??u00U.00 |d??S uo?e?ueouOO?euo?dO Sseudolggo |d??S uo?e?u8ouOO|euo?dO SSeluO?g40 Zd??S Patent Application Publication Dec. 18, 2008 Sheet 14 of 18 US 2008/0311640 A1 Patent Application Publication Dec. 18, 2008 Sheet 15 of 18 US 2008/0311640 A1 gleinfija Patent Application Publication Dec. 18, 2008 Sheet 16 of 18 US 2008/0311640 A1 ?BAOluÐ>}JO ºppolauoqueo einfil91 SSEuIO/g Patent Application Publication Dec. 18, 2008 Sheet 17 of 18 US 2008/0311640 A1 L?,eun61-I No.sae quoudinbº Patent Application Publication Dec. 18, 2008 Sheet 18 of 18 US 2008/0311640 A1 9.Infil8I, | - osequieq US 2008/0311640 A1 Dec. 18, 2008 ANAEROBC PRODUCTION OF HYDROGEN fermentation of the biomass have been selected by means of AND OTHER CHEMICAL PRODUCTS a Knowledge Management System, and selected and isolated using an isolation/enrichment system. FIELD OF THE INVENTION 0001. The methods and compositions described herein are Bacterial Strains directed to the production of hydrogen and other chemical 0009. In further or alternative embodiments of all aspects products via the anaerobic bacterial fermentation of biomass, described herein using bacterial strains to anaerobically fer and the production of hydrogen and other chemical products ment biomass and obtain chemical products therefrom, the via bacterial conversion of products obtained from anaerobic bacterial strains are substantially purified anaerobic bacteria fermentation. and such substantially purified bacterial strains are selected to anaerobically ferment the biomass, and in still further or BACKGROUND OF THE INVENTION alternative embodiments of all aspects, the Substantially puri 0002 Hydrogen has high energy content, with water being fied bacterial strains are selected from the group consisting of the product resulting from combustion of hydrogen with oxy Acetivibrio cellulolyticus, Acetivibrio cellulosolvens, Acetiv gen. As such, hydrogen represents a potentially ideal fuel ibrio ethanolgignens, Acetivibrio multivorans, Acetoanaero source. However, the use of hydrogen as a viable alternative bium noterae, Acetofilamentum rigidium, Acetogenium kivui, energy source remains challenging, because many methods Acetomicrobium faecale, Acetomicrobium flavidum, Aceto for producing hydrogen, such as water electrolysis, and petro thermus paucivorans, Acidaminobacter hydrogenoformans, leum reforming, are economically and energetically intense. Anaerobiospirillum succiniciproducens, Anaerobiospirillum ihomasii, Anaerorhabdus fircosa, Anaerovibrio burkinaben SUMMARY OF THE INVENTION sis, Anaerovibrio glycerini, Anaerovibrio lipolyticus, Atopo biunfossor; Atopobium minutum, Atopobium parvulum, Ato 0003. In one aspectare methods of preparing nutrients for pobium rimae, Atopobium vaginae, Bacteroides acidifaciens, use in anaerobic production of hydrogen. In another aspect Bacteroides amylophilus, Bacteroides asaccharolyticus are nutrients for use in the anaerobic production of hydrogen, Bacteroides bivius, Bacteroides buccae, Bacteroides bucca wherein the nutrients are prepared by methods, which lis, Bacteroides caccae Bacteroides capillosus, Bacteroides include, but are not limited to concentrating, sterilization and capillus, Bacteroides cellulosolvens, Bacteroides coagulans, deoxygenation. Bacteroides corporis, Bacteroides denticola, Bacteroides 0004. In one aspect are isolation/enrichment systems for disiens, Bacteroides distasonis, Bacteroides eggerthii, selecting bacteria for use in anaerobic hydrogen production, Bacteroides endodontalis, Bacteroides forsythus, Bacteroi wherein the bacterial strains are selected for their ability to des fragilis, Bacteroides fircosus, Bacteroides galacturoni utilize biomass as nutrients and produce hydrogen gas and cus, Bacteroides gingivalis, Bacteroides gracilis, Bacteroi other chemical products. In another aspect is an apparatus for des helcogenes, Bacteroides heparinolyticus, Bacteroides selecting bacteria for use in anaerobic hydrogen production hypermegas, Bacteroides intermedius, Bacteroides levi, comprising a sealed chamber capable of providing an appro Bacteroides loescheii, Bacteroides macacae, Bacteroides priate environment and growing conditions. melaminogenicus, Bacteroides meloninogenicus subsp. 0005. In one aspect is a Knowledge Management System intermedius, Bacteroides melaminogenicus subsp. macaca, used to identify bacteria for use in anaerobic production of Bacteroides melaminogenicus subsp. melaninogenicus, hydrogen from using biomass, wherein bacteria are identified Bacteroides merdae, Bacteroides microfitsus, Bacteroides by collecting appropriate information from various bacterial multiacidus, Bacteroides nodosus, Bacteroides ochraceus, species and deriving a probabilistic model from the informa Bacteroides oralis, Bacteroides Oris, Bacteroides Oulorum, tion. In another aspect is a computer program product to Bacteroides ovatus, Bacteroides pectinophilus, Bacteroides obtain candidate bacterial species for use in the anaerobic pentosaceus, Bacteroides pneumosintes, Bacteroides production of hydrogen and other chemical products from a polypragmatus, Bacteroides praeacutus, Bacteroides putre particular biomass. dinis, Bacteroides pyogenes, Bacteroides ruminicola, 0006. In one aspect are chemical products produced by Bacteroides ruminicola subsp. brevis, Bacteroides rumini anaerobically fermenting biomass with bacteria. In another cola subsp. ruminicola, Bacteroides salivosus, Bacteroides aspectare chemical products formed by admixing hydrogen, splanchnicus, Bacteroides stercoris, Bacteroides succino a starting material and a catalyst, wherein the hydrogen is genes, Bacteroides suis, Bacteroides tectus, Bacteroides ter produced by anaerobically fermenting a biomass with bacte mitidis, Bacteroides thetaiotaOmicron, Bacteroides unifor ria. In another aspectare chemical products formed by admix mis, Bacteroides ureolyticus, Bacteroides veroralis, ing a mineral and a volatile organic acid, wherein the Volatile Bacteroides vulgatus, Bacteroides xylanolyticus, Bacteroides organic acid is produced by anaerobically fermenting a bio zoogleoformans, Bifidobacterium adolescentis, Bifidobacte mass with bacteria.
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