DOCSLIB.ORG

Prevalence, Intensity, and Phylogenetic Analysis of Henneguya Piaractus and Myxobolus Cf. Colossomatis from Farmed Piaractus Mesopotamicus in Brazil

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Prevalence, Intensity, and Phylogenetic Analysis of Henneguya Piaractus and Myxobolus Cf. Colossomatis from Farmed Piaractus Mesopotamicus in Brazil Vol. 107: 129–139, 2013 DISEASES OF AQUATIC ORGANISMS Published December 12 doi: 10.3354/dao02668 Dis Aquat Org Prevalence, intensity, and phylogenetic analysis of Henneguya piaractus and Myxobolus cf. colossomatis from farmed Piaractus mesopotamicus in Brazil Maria Isabel Müller1,*, Edson Aparecido Adriano1,2, Paulo Sérgio Ceccarelli3, Marcia Ramos Monteiro da Silva4, Antonio Augusto Mendes Maia4, Marlene Tiduko Ueta1 1Department of Animal Biology, Institute of Biology, Universidade Estadual de Campinas, Rua Monteiro Lobato, 255, CEP: 13083-862, Campinas, SP, Brazil 2Department of Biological Sciences, Universidade Federal de São Paulo, CEP: 09972-270, Diadema, SP, Brazil 3National Research and Conservation Center for Continental Fish, Instituto Chico Mendes de Conservação da Biodiversidade, CEP: 13630-970, Pirassununga, SP, Brazil 4Department of Basic Sciences, Faculty of Animal Science and Food Engineering, Universidade de São Paulo, CEP: 13635-900, Pirassununga, SP, Brazil ABSTRACT: Henneguya piaractus and Myxobolus colossomatis (Myxosporea: Myxobolidae) are commonly found in the characid Piaractus mesopotamicus, an important fish farm species in Brazil. This paper describes the prevalence, mean intensity, molecular phylogeny, ultrastructure, and histology of H. piaractus and M. cf. colossomatis found infecting specimens of P. mesopotam- icus collected from fish farms in the state of São Paulo, Brazil. A total of 278 fish were collected from 3 fish farms between February 2008 and July 2010. Parasite prevalence and mean intensity varied throughout the study period, and according to location and year. A phylogenetic tree, plac- ing South American species in a global context, showed a clear tendency among myxosporean species to cluster according to host families. Ultrastructural analysis for M. cf. colossomatis showed the plasmodial wall with numerous projections toward host cells and phagocytic activity. Histopathological data showed hyperplasia caused by H. piaractus in highly infected fish. Histo- logical and ultrastructural analysis of H. piaractus showed results similar to those that have previ- ously been reported. KEY WORDS: Piaractus mesopotamicus · Henneguya piaractus · Myxobolus cf. colossomatis · Ultrastructure · Phylogeny · São Paulo Resale or republication not permitted without written consent of the publisher INTRODUCTION structure (Fiala & Bartosová 2010) that mainly infect aquatic hosts and have a wide host range (Feist & Due to the expansion of the aquaculture industry, Longshaw 2006). Interest in the group has increased which has been developing worldwide since the simultaneously with the development of aquaculture, 1990s (FAO 2012), fish disease has become an impor- as a number of species of the group can cause serious tant research area (Barassa et al. 2003). Myxozoa outbreaks of disease among farmed fish species comprise an important group of fish pathogens. This (Feist & Longshaw 2006). Species of the genera phylum is composed of highly specialized metazoan Myxo bolus and Henneguya are among the most parasites with an extremely reduced body size and common fish parasites (Eiras et al. 2005, Eiras & *Email: [email protected] © Inter-Research 2013 · www.int-res.com 130 Dis Aquat Org 107: 129–139, 2013 Adriano 2012). Here we evaluated myxosporeans with 2.5% glutaraldehyde in 0.1 M sodium cacody- found in farmed Piaractus meso potamicus (Holm- late buffer (pH 7.2) for scanning electron microscopy. berg 1887), a large characid commonly known in After washing in the same buffer, the preparations Brazil as pacu. It is naturally distributed in the were dehydrated in ethanol, critical-point dried with Paraná, Paraguay, and Uruguay basins (Godoy 1975) CO2, coated with metallic gold, and examined using and is one of the most important fish species in a JEOL JSM 35 microscope operating at 15 kV (Adri- Brazilian fish farming (Adriano et al. 2005). Pacu is ano et al. 2002a). the second most produced species native to South Plasmodia were fixed in 2.5% glutaraldehyde in America in Brazilian fish farming, with 21 245 t pro- 0.1 M cacodylate buffer (pH 7.4) for 12 h, washed in duced in 2010, together with another 21 621 t of a glucose-saline solution for 2 h and post-fixed in tambacu (a hybrid resulting from the crossing of OsO4, performed at 4°C, for transmission electron pacu and tambaqui Colossoma macropomum) (MPA microscopy observation. After dehydration in an ace- 2012). tone series, the material was embedded in EMbed The aim of this study was to evaluate the preva- 812 resin. Ultrathin sections double stained with lence and mean intensity of Henneguya piaractus uranyl acetate and lead citrate were examined using Martins et Souza, 1997 and Myxobolus cf. colossoma- an LEO 906 electron microscope operating at 60 kV tis Molnár et Békési, 1993. Morphological, histologi- (Adriano et al. 2005). cal, and ultrastructural analysis and 18S rRNA gene The contents of the plasmodia were collected for sequencing, together with phylogenetic analysis, molecular analysis in a 1.5 ml microcentrifuge tube were also performed. and fixed in 99% ethanol. DNA was extracted using the Qiagen Dneasy® Blood and Tissue kit, following the manufacturer’s instructions, with a final volume MATERIALS AND METHODS of 100 µl. DNA content was determined using a Nano Drop 2000 spectrophotometer (Thermo Scien- In total, 278 specimens of Piaractus mesopotamicus tific) at 260 nm. Polymerase chain reaction (PCR) (6.8 to 53.0 cm in length) were collected from 3 fish was performed in a final volume of 25 µl, containing farms located in the state of São Paulo from February 10 to 50 ng of extracted DNA, 1× Taq DNA Poly- 2008 to July 2010: 218 fish from the Pirassununga merase buffer (Invitrogen), 0.2 mmol of dNTP (Invit- farm (21° 55’ 52’’ S, 47° 22’ 29’’ W) between February rogen), 1.5 mmol of MgCl2, 0.2 pmol of each primer 2008 and July 2010, 50 fish from the Mogi Mirim farm (In vitrogen), 0.25 µl (1.25 U) of Taq DNA poly- (22° 28’ 17’’ S, 47° 00’ 40’’ W) be tween February 2009 merase (Invitrogen), and ultrapure (MilliQ) water. and July 2010, and 10 fish from the Itapira farm Fragments of the small subunit rDNA gene were (22° 26’ 11’’ S, 46° 49’ 20’’ W), in October 2010 only. amplified using the primer set ERIB1 (forward) and Fish were collected using nets and fishing rods and ACT1R (re verse), MYXGEN4f (forward), and ERIB10 transported alive to the laboratory, where they were (reverse; Table 1) (Barta et al. 1997, Andree et al. killed by transection of the spinal cord, before being 1999, Hallett & Diamant 2001, Diamant et al. 2004). measured and necropsied. The gills were immedi- An initial denaturation step was carried out at 95°C ately screened for myxozoans using a stereomicro- for 5 min, followed by 35 cycles of denaturation scope, and measurement of the spores was per- (95°C for 60 s), annealing (62°C for 60 s), and exten- formed in accordance with studies by Lom & Arthur sion (72°C for 120 s), and a final extended elonga- (1989). Measuring was performed using a computer tion step at 72°C for 5 min. PCR products were elec- equipped with Axivision 4.1 software, linked to an trophoresed in 1.0% agarose gel (Bio America) in a Axioplan 2 Zeiss microscope. TBE buffer (0.045 M Tris-borate, 0.001 M EDTA, pH Free spores were fixed in methanol, stained with 8.0), stained with ethidium bromide and analyzed in Giemsa solution (pH 7.2). A low viscosity mounting an FLA-3000 (Fugi) scanner. The size of the ampli- medium, CytosealTM, was used to create permanent fied fragments was estimated by comparison with slides. Fragments of gills containing cysts were fixed the 1 kb DNA Ladder (Invitrogen). Amplicons were in buffered 10% formalin for 24 h and placed in purified using the QIAquick® PCR Purification Kit paraffin for histopathological analysis. Sections (4 µm (Qiagen). The sequencing was prepared using the thick) were then stained with hematoxylin-eosin same primer pairs used in the PCR and also the (Adriano et al. 2002a). MC5-MC3 primer pair (Table 1) (Molnár et al. 2002) Spores were deposited on a cover slip coated with and was performed with the BigDye ® Terminator poly-L-lysine and fixed for 2 h at room temperature v3.1 cycle sequencing kit (Applied Biosystems) in Müller et al.: Myxosporeans in Piaractus mesopotamicus 131 Table 1. Primers used to amplify and sequence 18S fragments of Henneguya piaractus and Myxobolus cf. colossomatis. F: foward; R: reverse Primer Sequence (5’ to 3’) Source ERIB1 F ACC TGG TTG ATC CTG CCA G Barta et al. (1997) ACT1R R AAT TTC ACC TCT CGC TGC CA Hallett & Diamant (2001) MYXGEN4f F GTG CCT TGA ATA AAT CAG AG Diamant et al. (2004) ERIB10 R CTT CCG CAG GTT CAC CTA CGG Barta et al. (1997) MC5 F CCT GAG AAA CGG CTA CCA CAT CCA Molnár et al. (2002) MC3 R GAT TAG CCT GAC AGA TCA CTC CAC GA Molnár et al. (2002) an ABI 3730 DNA se quencing analyzer (Applied software (SAS Institute), and Duncan’s multiple Biosystems). range test was applied to compare the averages of For each sequence, a standard nucleotide−nucleo- prevalence and mean intensity of infection for loca- tide BLAST (blastn) search (Altschul et al. 1997) was tion, year, and species (p ≤ 0.05 significance level). conducted to verify similarity with other myxo - sporean sequences in GenBank. The partial se quen - ces of the 18S rDNA obtained from the species con- RESULTS sidered in the present study were visualized, edited, and aligned with BioEdit 7.1.3.0 (Hall 1999) software Henneguya piaractus (Figs. 1, 3, & 4) and Myxobo- using the ClustalW algorithm (Thompson et al. 1994). lus cf. colossomatis (Figs. 2, 3, & 5) were found to The phylogenetic positions of the partial 18S rDNA infect Piaractus mesopotamicus in all 3 of the evalu- gene of Henneguya piaractus and Myxobolus cf.
Load more

Recommended publications
  • Juizado Especial Cível E Criminal End.: Av
    JUIZADOS ESPECIAIS E INFORMAIS DE CONCILIAÇÃO E SEUS ANEXOS 2. COMARCAS DO INTERIOR ADAMANTINA – Juizado Especial Cível e Criminal End.: Av. Adhemar de Barros, 133 - Centro CEP: 17800-000 Fone: (18) 3521-1814 Fax: (18) 3521-1814 Horário de Atendimento aos advogados: das 09 às 18 horas Horário de Atendimento aos estagiários: das 09 às 18 horas Horário de Atendimento ao público: das 12:30 às 17 horas Horário de Triagem: das 12:30 às 17 horas ➢ UAAJ - FAC. ADAMANTINENSES INTEGRADAS End.: Av. Adhemar de Barros, 130 - Centro Fone: (18) 3522-2864 Fax: (18) 3502-7010 Horário de Funcionamento: das 08 às 18 horas Horário de Atendimento aos advogados: das 08 às 18 horas Horário de Atendimento ao Público: das 08 às 18 horas AGUAÍ – Juizado Especial Cível End.: Rua Joaquim Paula Cruz, 900 – Jd. Santa Úrsula CEP: 13860-000 Fone: (19) 3652-4388 Fax: (19) 3652-5328 Horário de Atendimento aos advogados: das 09 às 18 horas Horário de Atendimento aos estagiários: das 10 às 18 horas Horário de Atendimento ao público: das 12:30 às 17 horas Horário de Triagem: das 12:30 às 17 horas ÁGUAS DE LINDÓIA – Juizado Especial Cível e Criminal End.: Rua Francisco Spartani, 126 – Térreo – Jardim Le Vilette CEP: 13940-000 Fone: (19) 3824-1488 Fax: (19) 3824-1488 Horário de Atendimento aos advogados: das 09 às 18 horas Horário de Atendimento aos estagiários: das 10 às 18 horas Horário de Atendimento ao público: das 12:30 às 18 horas Horário de Triagem: das 12:30 às 17 horas AGUDOS – Juizado Especial Cível e Criminal End.: Rua Paulo Nelli, 276 – Santa Terezinha CEP: 17120-000 Fone: (14) 3262-3388 Fax: (14) 3262-1344 (Ofício Criminal) Horário de Atendimento aos advogados: das 09 às 18 horas Horário de Atendimento aos estagiários: das 10 às 18 horas Horário de Atendimento ao público: das 12:30 às 18 horas Horário de Triagem: das 12:30 às 17 horas ALTINÓPOLIS – Juizado Especial Cível e Criminal End.: Av.
    [Show full text]
  • Summary Report of Freshwater Nonindigenous Aquatic Species in U.S
    Summary Report of Freshwater Nonindigenous Aquatic Species in U.S. Fish and Wildlife Service Region 4—An Update April 2013 Prepared by: Pam L. Fuller, Amy J. Benson, and Matthew J. Cannister U.S. Geological Survey Southeast Ecological Science Center Gainesville, Florida Prepared for: U.S. Fish and Wildlife Service Southeast Region Atlanta, Georgia Cover Photos: Silver Carp, Hypophthalmichthys molitrix – Auburn University Giant Applesnail, Pomacea maculata – David Knott Straightedge Crayfish, Procambarus hayi – U.S. Forest Service i Table of Contents Table of Contents ...................................................................................................................................... ii List of Figures ............................................................................................................................................ v List of Tables ............................................................................................................................................ vi INTRODUCTION ............................................................................................................................................. 1 Overview of Region 4 Introductions Since 2000 ....................................................................................... 1 Format of Species Accounts ...................................................................................................................... 2 Explanation of Maps ................................................................................................................................
    [Show full text]
  • Endereços Cejuscs
    PODER JUDICIÁRIO TRIBUNAL DE JUSTIÇA DO ESTADO DE SÃO PAULO Núcleo Permanente de Métodos Consensuais de Solução de Conflitos Fórum João Mendes Junior, 13º andar – salas 1301/1311 E-mail: [email protected] CENTROS JUDICIÁRIOS DE SOLUÇÃO DE CONFLITOS E CIDADANIA – CEJUSCs ÍNDICE (Clicar na localidade desejada para obtenção do endereço, telefone e e-mail) SÃO PAULO – CAPITAL .............................................................................................................. 7 Cejusc Central – Fórum João Mendes Jr .................................................................................... 7 Butantã - Foro Regional XV ....................................................................................................... 8 Itaquera - Guaianazes - Foro Regional VII .................................................................................. 8 Jabaquara - Foro Regional III ..................................................................................................... 9 Lapa - Foro Regional IV.............................................................................................................. 9 Nossa Senhora do Ó - Foro Regional XII ..................................................................................... 9 Pinheiros - Foro Regional XI....................................................................................................... 9 Santana - Foro Regional I ........................................................................................................ 10 Santo Amaro - Foro
    [Show full text]
  • Diet Shift of Red Belly Pacu Piaractus Brachypomus (Cuvier, 1818) (Characiformes: Serrasalmidae), a Neotropical Fish, in the Sepik-Ramu River Basin, Papua New Guinea
    Neotropical Ichthyology, 12(4): 827-833, 2014 Copyright © 2014 Sociedade Brasileira de Ictiologia DOI: 10.1590/1982-0224-20130212 Diet shift of Red Belly Pacu Piaractus brachypomus (Cuvier, 1818) (Characiformes: Serrasalmidae), a Neotropical fish, in the Sepik-Ramu River Basin, Papua New Guinea Sandra Bibiana Correa1, Ricardo Betancur-R.2, 3, Bernard de Mérona4 and Jonathan W. Armbruster2 Introduction of fish species is a globally widespread practice that causes losses of native species and homogenization of diversity within and across continents. Diet assessments are important tools to depict the ecological function of species introduced into novel ecosystem and possible direct and indirect ecological effects. In this study, we compare the diet of Piaractus brachypomus, a mainly frugivorous Neotropical fish, introduced into the Sepik-Ramu River Basin (Papua New Guinea) nearly two decades ago, to that of similar size individuals from Neotropical populations in the Amazon and Orinoco River basins (South America). In contrast to native populations that feed mainly on terrestrial plants and invertebrates, the diet of introduced P. brachypomus is mainly composed of fish remains and aquatic plants, while terrestrial plants are frequently consumed but in relatively smaller amounts. These findings show that P. brachypomus has an inherently plastic diet that can be adjusted when displaced to a novel geographic area. While trophic plasticity increases the likelihood of a species to establish breeding populations after its introduction, it also reduces our ability to predict negative effects on native species. La introducción de peces es una práctica que se extiende globalmente y que causa perdida de especies nativas y homogenización de la diversidad dentro y entre continentes.
    [Show full text]
  • Field Guide to the Culture of Tambaqui (Colossoma Macropomum, Cuvier, 1816) Field Guide to the Culture of Tambaqui ( Colossoma Macropomum , Cuvier, 1816)
    ISSN 2070-7010 FAO 624 FISHERIES AND AQUACULTURE TECHNICAL PAPER 624 Field guide to the culture of tambaqui (Colossoma macropomum, Cuvier, 1816) Field guide to the culture of tambaqui ( Following a short introduction to the species and its closest commercially viable related species, namely pirapatinga (Piaractus brachypomus) and pacu (Piaractus mesopotamicus), this field guide provides practical information on the culture and reproduction of tambaqui (Colossoma macropomum). Colossoma macropomum As a field guide it aims to support the understanding and dissemination of applicable technologies for the culture and reproduction of tambaqui, i.e. what should be done – as well as when and how it should be done – in order to achieve success in the artificial propagation as well as the fingerling and table fish production stages. The concise technical descriptions in this guide are accompanied by self-explanatory illustrations and a reader-friendly glossary of technical terms, which is important for tambaqui aquaculture farmers. , Cuvier, 1816) ISBN 978-92-5-131242-1 ISSN 2070-7010 978 9251 312421 FAO CA2955EN/1/01.19 Cover photographs: ©FAO/András Woynárovich. Illustrations and photos in this Technical Paper are courtesy of András Woynárovich. Images and photos courtesy of other authors are indicated separately. FAO FISHERIES AND Field guide AQUACULTURE TECHNICAL to the culture of tambaqui PAPER (Colossoma macropomum, 624 Cuvier, 1816) by András Woynárovich FAO Consultant Budapest, Hungary and Raymon Van Anrooy Fishery Industry Officer Fisheries and Aquaculture Department Rome, Italy FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS Rome, 2019 Required citation Woynárovich, A. and Van Anrooy, R. 2019. Field guide to the culture of tambaqui (Colossoma macropomum, Cuvier, 1816).
    [Show full text]
  • MUNICÍPIO MÓDULO FISCAL - MF (Ha) (Ha)
    QUATRO MFs MUNICÍPIO MÓDULO FISCAL - MF (ha) (ha) ADAMANTINA 20 80 ADOLFO 22 88 AGUAÍ 18 72 ÁGUAS DA PRATA 22 88 ÁGUAS DE LINDÓIA 16 64 ÁGUAS DE SANTA BÁRBARA 30 120 ÁGUAS DE SÃO PEDRO 18 72 AGUDOS 12 48 ALAMBARI 22 88 ALFREDO MARCONDES 22 88 ALTAIR 28 112 ALTINÓPOLIS 22 88 ALTO ALEGRE 30 120 ALUMÍNIO 12 48 ÁLVARES FLORENCE 28 112 ÁLVARES MACHADO 22 88 ÁLVARO DE CARVALHO 14 56 ALVINLÂNDIA 14 56 AMERICANA 12 48 AMÉRICO BRASILIENSE 12 48 AMÉRICO DE CAMPOS 30 120 AMPARO 20 80 ANALÂNDIA 18 72 ANDRADINA 30 120 ANGATUBA 22 88 ANHEMBI 30 120 ANHUMAS 24 96 APARECIDA 24 96 APARECIDA D'OESTE 30 120 APIAÍ 16 64 ARAÇARIGUAMA 12 48 ARAÇATUBA 30 120 ARAÇOIABA DA SERRA 12 48 ARAMINA 20 80 ARANDU 22 88 ARAPEÍ 24 96 ARARAQUARA 12 48 ARARAS 10 40 ARCO-ÍRIS 20 80 AREALVA 14 56 AREIAS 35 140 AREIÓPOLIS 16 64 ARIRANHA 16 64 ARTUR NOGUEIRA 10 40 ARUJÁ 5 20 ASPÁSIA 26 104 ASSIS 20 80 ATIBAIA 16 64 AURIFLAMA 35 140 AVAÍ 14 56 AVANHANDAVA 30 120 AVARÉ 30 120 BADY BASSITT 16 64 BALBINOS 20 80 BÁLSAMO 20 80 BANANAL 24 96 BARÃO DE ANTONINA 20 80 BARBOSA 30 120 BARIRI 16 64 BARRA BONITA 14 56 BARRA DO CHAPÉU 16 64 BARRA DO TURVO 16 64 BARRETOS 22 88 BARRINHA 12 48 BARUERI 7 28 BASTOS 16 64 BATATAIS 22 88 BAURU 12 48 BEBEDOURO 14 56 BENTO DE ABREU 30 120 BERNARDINO DE CAMPOS 20 80 BERTIOGA 10 40 BILAC 30 120 BIRIGUI 30 120 BIRITIBA-MIRIM 5 20 BOA ESPERANÇA DO SUL 12 48 BOCAINA 16 64 BOFETE 20 80 BOITUVA 18 72 BOM JESUS DOS PERDÕES 16 64 BOM SUCESSO DE ITARARÉ 20 80 BORÁ 20 80 BORACÉIA 16 64 BORBOREMA 16 64 BOREBI 12 48 BOTUCATU 20 80 BRAGANÇA PAULISTA 16 64 BRAÚNA
    [Show full text]
  • Locais E Vinculação Da Sfpc Da 2ª Região Militar
    LOCAIS E VINCULAÇÃO DA SFPC DA 2ª REGIÃO MILITAR UNIDADE ENDEREÇO CIDADE CONTATO E-MAIL CIDADE VINCULADA ARUJA BARUERI BIRITIBA-MIRIM CARAPICUIBA COTIA DIADEMA EMBU DAS ARTES EMBU-GUACU FERRAZ DE VASCONCELOS GUARAREMA GUARULHOS ITAPECERICA DA SERRA ITAPEVI ITAQUAQUECETUBA JANDIRA SFPC/2.00 - 2ª JUQUITIBA Av. Sargento Mario Kozel Filho, 222 SAO PAULO (11) 3888-5200 [email protected] MAUA REGIÃO MILITAR MOGI DAS CRUZES OSASCO POA RIBEIRAO PIRES RIO GRANDE DA SERRA SALESOPOLIS SANTA ISABEL SANTANA DE PARNAIBA SANTO ANDRE SAO BERNARDO DO CAMPO SAO CAETANO DO SUL SAO LOURENCO DA SERRA SAO PAULO SUZANO TABOAO DA SERRA BARRA DO TURVO BERTIOGA CAJATI CANANEIA CUBATAO ELDORADO GUARUJA IGUAPE ILHA COMPRIDA ITANHAEM SFPC/2.01 - CMDO 1ª ITARIRI (13) 3354-2888 / 3354-2889 / Praia do Manduba, s/n - Fortes dos Andradas GUARUJA [email protected] JACUPIRANGA BDA AAAÉ / 2º 2916 / 2817 / 2569 JUQUIA GAAAe/ 2º BIL MIRACATU MONGAGUA PARIQUERA-ACU PEDRO DE TOLEDO PERUIBE PRAIA GRANDE REGISTRO SANTOS SAO VICENTE SETE BARRAS CACONDE CASA BRANCA DIVINOLANDIA ITOBI LUIS ANTONIO MOCOCA SFPC/2.02 - 13º RC PIRASSUNUNGA Av. Newton Prado Prado, 2251 PIRASSUNUNGA (19) 3561-3777 / 3561-5333 [email protected] PORTO FERREIRA MEC SANTA CRUZ DAS PALMEIRAS SANTA RITA DO PASSA QUATRO SANTA ROSA DE VITERBO SAO JOSE DO RIO PARDO SAO SIMAO TAMBAU TAPIRATIBA CACAPAVA CARAGUATATUBA IGARATA ILHABELA SFPC/2.03 - CMDO 12ª JACAREI Rua José Bonifácio, 33 - Jd Rafael CACAPAVA (12) 3653-2692 [email protected] JAMBEIRO BDA INF L MONTEIRO LOBATO PARAIBUNA SANTA BRANCA SAO JOSE DOS CAMPOS SAO SEBASTIAO CAMPOS DO JORDAO LAGOINHA NATIVIDADE DA SERRA PINDAMONHANGABA REDENCAO DA SERRA ROSEIRA SFPC/2.04 - 2º BECMB Praça Padre João de Faria Fialho, 46 - Centro PINDAMONHANGABA (12) 3648-3114 [email protected] SANTO ANTONIO DO PINHAL SAO BENTO DO SAPUCAI SAO LUIS DO PARAITINGA TAUBATE TREMEMBE UBATUBA APARECIDA ARAPEI AREIAS BANANAL CACHOEIRA PAULISTA CRUZEIRO CUNHA SFPC/2.05 - 5º BIL Av.
    [Show full text]
  • Prefeitura Municipal De Catanduva
    www.catanduva.sp.gov.br Quinta-Feira, 19 de Março de 2020 Ano II | Edição nº 1522 Página 1 de 30 Sumário Licitações e Contratos .................................................. 2 ATA DE REGISTRO DE PREÇOS ............................................. 2 ADITAMENTO CONTRATUAL ............................................... 10 ADITAMENTO DE ATA DE REGISTRO DE PREÇOS ............................... 11 HOMOLOGAÇÃO ....................................................... 12 JULGAMENTO DE CLASSIFICAÇÃO .......................................... 13 JULGAMENTO DE HABILITAÇÃO ............................................ 14 PRAZO DE RECURSO .................................................... 16 Secretaria de Finanças ................................................ 17 NOTIFICAÇÃO PARTIDOS POLÍTICOS, SINDICATOS DE TRABALHADORES E ENTIDADES EMPRESARIAIS ........................................................ 17 Secretaria de Educação ................................................ 18 RESOLUÇÃO SME Nº 09, DE 19 DE MARÇO DE 2020 ............................ 18 Secretaria de Assistência Social ........................................ 19 CANCELAMENTO REUNIÃO ORDINÁRIA CMDCA ................................ 19 Superintendência de Água e Esgoto de Catanduva ....................... 20 ABERTURA DE LICITAÇÃO ................................................ 20 ADITAMENTO CONTRATUAL ............................................... 21 CONTRATO ........................................................... 22 HOMOLOGAÇÃO ......................................................
    [Show full text]
  • A Review on the Fishfauna of Mogi-Guaçu River Basin: a Century of Studies Uma Revisão Da Ictiofauna Da Bacia Do Rio Mogi-Guaçu Em Um Século De Estudos Meschiatti, AJ
    A review on the fishfauna of Mogi-Guaçu River basin: a century of studies Uma revisão da ictiofauna da bacia do Rio Mogi-Guaçu em um século de estudos Meschiatti, AJ. and Arcifa, MS. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo – USP, Av. Bandeirantes, 3900, CEP 14040-901, Ribeirão Preto, SP, Brazil e-mail: [email protected], [email protected] Abstract: Aim: The aims of this review were to synthesize information scattered in the literature, during ca. a century of studies on the fishfauna composition of Mogi-Guaçu River basin; the species distribution in the habitats, such as river, tributaries, and floodplain lakes; the diet, feding habits, migration, growth and reproduction of several species; and the influence of environmental degradation. Material and Methods: Analysis and synthesis of information obtained in the literature from 1900 to 2008 and comparison of similarities of the fishfauna composition in the habitats were done. The first studies on fish migration in South America using tagging and recapture were performed in Mogi-Guaçu River. Results: A hundred and fifty species were recorded in the river basin, distributed in the river, tributaries, floodplain lakes and a reservoir. Data on the diet of 73 species and the reproduction of 27 species were included. The similarity is higher between river and tributaries (47%) than between tributaries and lakes (42%) and river and lakes (37%). Conclusions: Several factors, such as sand dredging in the river channel, domestic and industrial sewage, pesticides and fertilizers used in the agriculture contribute to the environmental degradation, reducing nurseries and suitable conditions for the maintenance of populations.
    [Show full text]
  • Transcriptome Profiling of Pacu (Piaractus Mesopotamicus) Challenged with Pathogenic Aeromonas Hydrophila: Inference on Immune Gene Response
    Edinburgh Research Explorer Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response Citation for published version: Mastrochirico Filho, VA, Hata, M, Kuradomi, RY, de Freitas, MV, Ariede , RB, Pinheiro, DG, Robledo, D, Houston, R & Hashimoto, DT 2020, 'Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response', Frontiers in genetics. https://doi.org/10.3389/fgene.2020.00604 Digital Object Identifier (DOI): 10.3389/fgene.2020.00604 Link: Link to publication record in Edinburgh Research Explorer Document Version: Publisher's PDF, also known as Version of record Published In: Frontiers in genetics General rights Copyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy The University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorer content complies with UK legislation. If you believe that the public display of this file breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Download date: 04. Oct. 2021 fgene-11-00604 June 5, 2020 Time: 19:40 # 1 ORIGINAL RESEARCH published: 09 June 2020 doi: 10.3389/fgene.2020.00604
    [Show full text]
  • Fish Species
    Fish Species Dorado or Golden Dorado- Classification: Kingdom: Animalia Phylum: Chordata Class: Actinopterygii Order: Characiformes Family: Characidae Genus: Salminus Species: maxillosus Binomial name: Salminus maxillosus Others: Salminus cuvieri, Salminus brasiliensis Characteristics: The dorado has been often referred to as the “tigre de rio” or jaguar of the river to be more accurate. They possess a bright golden coloration, accented by rows of tiny small back dots running along their powerful streamlined bodies. Their fins have a bright red coloration and the caudal fin has a black bar along the middle through the caudal peduncle. Their massive jaws are equipped with rows of razor sharp pointy teeth, living up quite accurately to its local nickname. Dorado can reach upwards of 100cm in length and weigh as much as 45lbs (20.5kgs), more commonly in the 22-33lb (10-15kg) range. Tabarana, the silver dorado- Classification- Kingdom: Animalia Phylum: Chordata Class: Actinopterygii Order: Characiformes Family: Characidae Genus: Salminus Species: hilarii Binomial name: Salminus hilarii Characteristics: This species is a close relative of the dorado, and shares many of its morphological features. However, this fish is smaller in average size, and has less girth. It is silver with bright orange specks between scales, and black and red accents on the caudal fin, as well as black accents on the dorsal and anal fins. This is why it is called the “white or silver dorado” in much of its range. This species is quite aggressive and preys upon smaller tetras and other baitfish such as sabalo (Prochilodus sp.). Its sharp teeth make quick work of the smaller characins in the river system.
    [Show full text]
  • Integrative Micrornaome Analysis of Skeletal Muscle of Colossoma Macropomum (Tambaqui), Piaractus Mesopotamicus (Pacu), And
    Fantinatti et al. BMC Genomics (2021) 22:237 https://doi.org/10.1186/s12864-021-07513-5 RESEARCH ARTICLE Open Access Integrative microRNAome analysis of skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu), and the hybrid tambacu, based on next- generation sequencing data Bruno E. A. Fantinatti1,2,3, Erika S. Perez1, Bruna T. T. Zanella1, Jéssica S. Valente1, Tassiana G. de Paula1, Edson A. Mareco4, Robson F. Carvalho1, Silvano Piazza3, Michela A. Denti3 and Maeli Dal-Pai-Silva1* Abstract Background: Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu) are good fish species for aquaculture. The tambacu, individuals originating from the induced hybridization of the female tambaqui with the male pacu, present rapid growth and robustness, characteristics which have made the tambacu a good choice for Brazilian fish farms. Here, we used small RNA sequencing to examine global miRNA expression in the genotypes pacu (PC), tambaqui (TQ), and hybrid tambacu (TC), (Juveniles, n = 5 per genotype), to better understand the relationship between tambacu and its parental species, and also to clarify the mechanisms involved in tambacu muscle growth and maintenance based on miRNAs expression. Results: Regarding differentially expressed (DE) miRNAs between the three genotypes, we observed 8 upregulated and 7 downregulated miRNAs considering TC vs. PC; 14 miRNAs were upregulated and 10 were downregulated considering TC vs. TQ, and 15 miRNAs upregulated and 9 were downregulated considering PC vs. TQ. The majority of the miRNAs showed specific regulation for each genotype pair, and no miRNA were shared between the 3 genotype pairs, in both up- and down-regulated miRNAs. Considering only the miRNAs with validated target genes, we observed the miRNAs miR-144-3p, miR-138-5p, miR-206-3p, and miR-499-5p.
    [Show full text]