Introducing C-Fern (Ceretopteris richardii)
Examination of the young Sporophyte Stage!! Haploid (n) Diploid (2n) Sperm (n) Mitosis and development Female gametangium (n)
Gametophyte (n) Spores (n) Egg (n)
Meiosis Fertilization
Clusters of sporangia 2 Zygote (2n) New sporophyte (2n)
3 Mitosis and development
Mature sporophyte Fig. 17-5-1 Key 1 Haploid (n) Sperm (n) Diploid (2n)
Archegonium (female gametangium) (n) Gametophyte (n)
Egg (n)
Fertilization Fig. 17-5-2 Key 1 Haploid (n) Sperm (n) Diploid (2n)
Female gametangium (n)
Gametophyte (n)
Egg (n)
Fertilization
2 Zygote (2n) Fig. 17-5-3 Key 1 Haploid (n) Sperm (n) Diploid (2n)
Female gametangium (n)
Gametophyte (n)
Egg (n)
Fertilization
Clusters of 4 sporangia 2 Zygote (2n) New sporophyte (2n)
3 Mitosis and development
Mature sporophyte Fig. 17-5-4 Key 1 Haploid (n) Sperm (n) Diploid (2n)
5 Mitosis and development Female gametangium (n)
Gametophyte (n) Spores (n) Egg (n)
Meiosis Fertilization
Clusters of 4 sporangia 2 Zygote (2n) New sporophyte (2n)
3 Mitosis and development
Mature sporophyte Young Sporophyte
Product of a fertilization event!
A = young sporophyte B = gametophyte Fertilization in C-fern Fertilization in C-fern! What are all the possible fertilization events?
"#$%!#&! Complete this table in your worksheet before '%()*+,%!)-!! examining your plate.! %''!
P! P! PP! WILD! WILD!
P! p!
p! P!
p! p!
P = wild allele p = mutant allele! Fertilization P p Events in P C-Fern P PP Pp
P p
Pp pp
P p p Examine Phenotypes of Sporophytes •! ./#$0(%!"#!$%&'&%()*+$!#(1!2%3)21!*4%!(5$6%2!)-! 7081!#(1!$5*#(*!&,)2),4+*%&! •! 94%(:!1%*%2$0(%!*4%!,2),)2;)(!)-!7081!#(1!$5*#(*! &,)2),4+*%&! •! <)!+)52!)6&%2=%1!2%&58*&!$#*34!+)52!,2%103;)(&>! •! ?%3)21!+)52!2%&58*&!)(!*4%!6)#21@!A)5!7088!(%%1!*4%! 0(10=015#8!#(1!,))8%1!1#*#!*)!3)$,8%*%!*4%!8#6!2%,)2*@! Wild Sporophytes: What are the phenotypes of the gametophytes on which they grow? Count ten randomly selected wild sporophytes indicating the type of gametophyte it is growing on. Do your results support your predictions?
Mutant Sporophytes: What are the phenotypes of the gametophytes on which they grow? ! Count ten randomly selected mutant sporophytes indicating the type of gametophyte it is growing on. Do your results support your predictions? Take images of the following:
•! 7081!&,)2),4+*%!)(!7081!'#$%*),4+*%! •! 7081!&,)2),4+*%!)(!$5*#(*!'#$%*),4+*%! •! $5*#(*!&,)2),4+*%!)(!$5*#(*!'#$%*),4+*%!
!B-!+)5!C(1!#!$5*#(*!&,)2),4+*%!)(!#!7081! '#$%*),4+*%!@!@!@!D4#*>>>! If you did not take pictures of gametophytes during the last lab…
A)5!3#(!&;88!*#E%!*4%$!*)1#+! A)5!(%%1F!! •! 7081!#(1!$5*#(*!$#8%!'#$%*),4+*%&! •! 7081!#(1!$5*#(*!4%2$#,42)10;3! '#$%*),4+*%&! Due next week: C-Fern Lab Report
G!
H! I!