Invertase from baker's (S. cerevisiae)

Catalog Number I4504 Storage Temperature –20 °C

CAS RN 9001-57-4 Reported KM values are 25 mM for and EC 3.2.1.26 150 mM for raffinose. At high substrate concentrations Synonyms: Saccharase, b-Fructofuranosidase, (1 M), invertase exhibits activity by b-D-Fructofuranoside fructohydrolase transferring the b-D-fructofuranosyl residue to primary including ethanol, methanol, and n-propanol. Product Description Invertase does not require any activators and is Molecular mass:1 270 kDa inhibited by iodine, Zn2+, and Hg2+.1,3 Extinction coefficient:1 E1% = 23.0 (280 nm) pI:2 3.4–4.4 Precautions and Disclaimer This product is for R&D use only, not for drug, Invertase from baker's yeast is a glycoprotein household, or other uses. Please consult the Material containing 50% mannan and 2-3% glucosamine. During Safety Data Sheet for information regarding hazards purification, a small percentage of the may and safe handling practices. have part of the carbohydrate moiety removed leaving a heterogeneous enzyme with a lower or even negligible Preparation Instructions mannan content. This difference in carbohydrate This enzyme is soluble in water (10 mg/ml), yielding a content has led to the conclusion that two forms of the clear solution. enzyme exist. An external invertase (predominant form), carbohydrate containing, located outside of the References cell membrane and an internal invertase, which is 1. Lampen, J.O., The , Boyer, P.D., ed., located entirely within the cytoplasm and devoid of any Academic Press (New York, NY:1971), carbohydrate.1,3 pp. 291-303. 2. Righetti, P.G., and Caravaggio, T., Isoelectric Invertase catalyzes the following reaction: points and molecular weights of proteins. J. Chromatogr., 127, 1-28 (1976).

Sucrose + H2O ® + 3. Goldstein, A., and Lampen, J.O., b-D-fructofuranoside fructohydrolase from yeast. Raffinose and methyl-b-D-fructofuranoside may also be Methods Enzymol., 42, 504-511 (1975). utilized as substrates. TMG,AJH,MAM 03/13-1

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