Product datasheet Anti-MAP2K4 Antibody Catalog Number: A01725-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-MAP2K4 Antibody Gene Name MAP2K4 Source Rabbit IgG Species Reactivity human,rat,mouse Tested Application WB Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human MEK4(360-378aa ELLKHPFILMYEERAVEVA), different from the related mouse and rat sequences by one amino acid. Purification Immunogen affinity purified. Observed MW 44KD Dilution Ratios Western blot: 1:500-2000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Dual specificity mitogen-activated protein kinase kinase 4(MAP2K4), also called SEK1 or JNKK1, is an enzyme that in humans is encoded by the MAP2K4 gene. It is mapped to 17p12. This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK1. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. MAP2K4 was a specific activator of JNK1, JNK2, and p38, and it has been shown to interact with FLNC, MAPK8, MAPK8IP3 and AKT1.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-MAP2K4 Antibody Catalog Number: A01725-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot analysis of Anti-MAP2K4 antibody (A01725-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat skeletal muscle tissue lysates,Lane 2: HELA whole cell lysates,Lane 3: A549 whole cell lysates,Lane 4: MM231 whole cell lysates,Lane 5: CEM whole cell lysates,Use rabbit Anti- MAP2K4 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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