Influence of DE-value on the physicochemical properties of for melt extrusion processes Natalia Castro Gutierrez, Vanessa Durrieu, Christine Raynaud, Antoine Rouilly

To cite this version:

Natalia Castro Gutierrez, Vanessa Durrieu, Christine Raynaud, Antoine Rouilly. Influence of DE- value on the physicochemical properties of maltodextrin for melt extrusion processes. Polymers, Elsevier, 2016, 144, pp.464-473. ￿10.1016/j.carbpol.2016.03.004￿. ￿hal-02083687￿

HAL Id: hal-02083687 https://hal.archives-ouvertes.fr/hal-02083687 Submitted on 29 Mar 2019

HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés.

OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible

This is an author’s version published in: http://oatao.univ-toulouse.fr/23384

Official URL: https://doi.org/10.1016/j.carbpol.2016.03.004

To cite this version:

Castro Gutierrez, Natalia and Durrieu, Vanessa and Raynaud, Christine and Rouilly, Antoine Influence of DE-value on the physicochemical properties of maltodextrin for melt extrusion processes. (2016) Carbohydrate Polymers, 144. 464-473. ISSN 0144-8617

Any correspondence concerning this service should be sent to the repository administrator: [email protected] Influence of DE•value on the physicochemical properties of maltodextrin for melt extrusion processes

Natalia Castro a,b, Vanessa Durrieu a,b, Christine Raynaud a,b, Antoine Rouilly a,b,∗ a Université de Toulouse, INP•ENSIACET, LCA (Laboratoire de Chimie Agro•industrielle), F•31030 Toulouse, France b INRA, UMR•1010•CAI, F•31030 Toulouse, France a r t i c l e i n f o a b s t r a c t

Keywords: In this study, five different types of maltodextrins (DE•2, DE•6, DE•12, DE•17 and DE•19) were character• value ized for the physico•chemical properties. TGA, DVS and SEC analyses were carried out and additionally Apparent viscosity apparent melt•viscosity (in a micro•extruder) and the glass transition temperature (analyzed by DMA) of Glass transition temperature maltodextrin/plasticizer mixtures were also measured in order to evaluate both the effect of plasticizer Water sorption isotherm nature and content and the effect of the DE•value. For this, three plasticizing agents were compared: Molecular weight distribution water, d•sorbitol and glycerin. The adsorption isotherms showed that depending on the DE•value and the relative humidity they were exposed to, different behavior could be obtained. For example, for rel• ative humidities below 60% RH maltodextrin DE•2 was the least hygroscopic. And on the contrary for relative humidities above 75% RH maltodextrin DE•2 was the most hygroscopic. The rheology measure• ments showed that the viscosity decreased with the increase of the DE•value and with the plasticizer content, as expected. On the contrary, no direct correlation could be established between the DE•value and the glass transition temperature. These results demonstrated that to predict maltodextrins behavior and to better adapt the process conditions, combined analyses are mandatory as the DE•value alone is not sufficient. The most compelling evidence was obtained by size exclusion chromatography, which pointed out that maltodextrins had a bimodal molecular weight distribution composed of high and low molecular weight oligo•saccharides. Indeed, maltodextrins are highly polydisperse materials (i.e. poly• dispersity index ranging from 5 to 12) and that should be the reason why such distinct behaviors were observed in some of the physico•chemical analyses that were preformed.

1. Introduction todextrins are great film forming and texturizing agents, as they can increase viscosity, retard crystallization or decrease stickiness Maltodextrins are obtained from the acid and/or enzymatic and hygroscopicity of a mixture but also improve shelf•life stabil• controlled of . Maltodextrins are composed of ity of food matrices (Roos & Karel, 1991). Maltodextrins are popular d• units connected by (1–4) glucosidic linkage to give in the food industry not only for all the previous reasons but also d•glucose polymers of variable length and therefore different because they are highly soluble in water and non•sweet compared molecular weight. The number of the reducing content is to classical (Raja, Sankarikutty, Sreekumar, Jayalekshmy, defined by the dextrose equivalent value (DE•value), which is cal• & Narayanan, 1989; Schebor, Mazzobre, & Buera, 2010). Not to culated on a dry weight basis. Maltodextrins are a mixture of mention that maltodextrins are odor•, color• and tasteless so saccharides with a DE•value ranging from 3 to 20. Starch is associ• they appear as the best option to be employed as encapsulating ated to a DE•value of zero, and glucose to a DE•value of 100 (Dokic, agents either by spray•drying or twin•screw extrusion. Nowadays, Jakovljevic, & Dokic•Baucal, 1998; Levine & Slade, 1986). maltodextrins are used as the main ingredient rather than addi• Maltodextrins are one of the most common compounds used in tive for the elaboration of bio•based materials by melt extrusion the cosmetic, food and pharmaceutical domain. It can be employed (Bouquerand, Maio, Meyer, & Normand, 2008; Castro et al., 2016; as the main ingredient of a formulation or as an additive. Mal• Tackenberg, Marmann, Thommes, Schuchmann, & Kleinebudde, 2014). The key for a successful encapsulation of an active compound is based on the understanding of the physicochemical properties ∗ Corresponding author. Fax: +33 5 34 32 35 97. of the wall material employed and therefore the adaptability of E•mail address: [email protected] (A. Rouilly). http://dx.doi.org/10.1016/j.carbpol.2016.03.004 the process conditions and of the technology to be used. For mal• 2.3. Size exclusion chromatography todextrins, the main problem is the lack of experimental data concerning the physicochemical properties of these raw materials. SEC analyses were performed using a Dionex (Voisins le Breton• Actually, there are more mathematical models allowing predicting neux, France) size exclusion chromatography (SEC) equipped with the behavior of some of the physicochemical properties than stud• a high•sensitivity inverse refractive index detector Prostar 350/352 ies measuring them because of the rigidity and brittleness of these from Varian Analytical Instruments (Walnut, C.A., USA). •based materials. The average molecular weights of maltodextrins were deter• Therefore in order to better understand maltodextrins, the aim mined by gel permeation chromatography (GPC) on a PL of this paper was to determine in the first place the molecular aquagel•OH 50 columns. The column system was composed of characteristics (molecular weight distribution, sorption isotherm, three columns; 2 Agilent PL aquagel•OH 30 8 ␮m, 7.5 × 300 mm apparent viscosity, and glass transition temperature) of five differ• (p/n 1120–6830 Polymer Laboratories Ltd., Church Stretton, UK) ent grades of pure maltodextrins; and in a second place, to analyze and a PLgel precolumn. The column oven temperature was set at the effect of the type and the amount of plasticizer on the apparent 30 ◦C. The eluents were 0.02 M NaCl in 0.005 M sodium phosphate viscosity and glass transition temperature of these mixtures. Ergo, buffer Sigma–Aldrich (St Quentin Fallavier, France), at pH 7 and the formulations herein studied can be adapted to the principal prepared as the protocol described by Ma et al. (2012). encapsulation technologies. Thus, tuning the formulation upstream External calibration was made with Pullulan standards, from can for instance improve the flowability of the mixture inside an Polymer Laboratories (Marseille, France), with specific average extruder, and control the properties of the final maltodextrin•based molecular weights ranging from 360 and 380,000 Da, dissolved in products. 0.005 M sodium phosphate buffer with 0.02 M NaCl, pH 7.5. The results were treated by Chromeleon software in order to obtain the number average (Mn), the weight average (Mw) molec• 2. Materials and methods ular weights and the polydispersity index (Ip) of each analyzed sample. All samples were run in triplicates. 2.1. Raw materials

Roquettes Frères (Lestrem, France) supplied maltodextrins with 2.4. Determination of the moisture content different dextrose equivalent (Glucidex•2, Glucidex•6, Glucidex IT•12, Glucidex IT•19 and Glucidex 17). These maltodextrins are Moisture content of the samples was determined by gravimetric obtained by controlled hydrolysis of native corn•starch. The main method (NF•V•ISO03•921). One gram of each sample was weighted ◦ difference between these two ranges of product is based on the and left to dry in an oven at 103 ± 2 C for 24 h until there were no powder particle size. Glucidex•IT has bigger particle size, providing mass variations of the sample. Measures were run in triplicates for a better solubilization and free•flowing properties. Two plasti• each sample. cizers were employed, glycerin (CAS: 56•81•5, MW = 92 g mol−1) d −1 and •sorbitol (CAS: 50•70•4, MW = 182 g mol ) both supplied 2.5. Dynamic vapor sorption analyses (DVS) by Sigma Aldrich (St Quentin Fallavier, France). Reagents used for the dextrose titration and for size exclusion chromatogra• Water sorption isotherms were performed on a Dynamic Vapor phy were also provided by Sigma–Aldrich: Copper (II) sulphate Sorption (DVS) Advantage System from Surface Measurement Sys• −1 pentahydrate (CAS: 7758•99•8, MW = 249.69 g mol ), Methylene tems (Alperton, UK). This machine is equipped with a very accurate −1 blue (CAS: 61•73•4, MW = 319.85 g mol ), potassium sodium tar• recording microbalance, able to measure changes in the sample −1 trate tetrahydrate (CAS: 6381•59•5, MW = 282.22 g mol ), sodium mass as low as 0.1 ␮g. Samples were exposed to a constant tem• −1 hydroxide (CAS: 1310•73•2, MW: 40 g mol ), Di•sodium hydrogen perature (25 ◦C) and programmed relative humidities varying from −1 phosphate (CAS: 10028•24•7, MW = 177.99 g mol ), sodium phos• 0 to 90% divided into 15% increments (14 steps). A mixture of dry −1 phate (CAS: 10049•21•5, MW = 138.0 g mol ) and sodium chloride and moisture•saturated nitrogen flowing over the samples assured (CAS: 7647•14•5, MW = 58.44). the changes in the relative humidity of the DVS•chamber. Ten mil• ligrams of the sample were placed inside the chamber and before starting the data acquisition, all the samples were dried for 300 min 2.2. Determination of the dextrose equivalent value of under a stream of dry nitrogen (0% RH) at 103 ◦C in order to obtain maltodextrins the dry weight. Equilibrium was achieved, when the changes in the mass of the sample were lower than 5.10−3% min−1. The DE•value were measured by the Hagedorn•Jensen method (Callow, 1930) in order to confirm the dextrose equivalent value established by the manufact