Anti-ZNF148 Antibody Rabbit Polyclonal Antibody to ZNF148 Catalog # AP60420

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Anti-ZNF148 Antibody Rabbit polyclonal antibody to ZNF148 Catalog # AP60420

Specification

Anti-ZNF148 Antibody - Product Information

Application WB, IH, IF Primary Accession Q9UQR1 Reactivity Human, Bovine Host Rabbit Clonality Polyclonal Calculated MW 88976

Anti-ZNF148 Antibody - Additional Information

Gene ID 7707

Other Names ZBP89; Zinc finger protein 148; Western blot analysis of ZNF148 expression Transcription factor ZBP-89; Zinc finger in HEK293T (A), H446 (B) whole cell lysates. DNA-binding protein 89

Target/Specificity Recognizes endogenous levels of ZNF148 protein.

Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) IF~~WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Format Liquid in 0.42% Potassium phosphate, Immunohistochemical analysis of ZNF148 0.87% Sodium chloride, pH 7.3, 30% staining in human breast cancer formalin glycerol, and 0.01% sodium azide. fixed paraffin embedded tissue section. The section was pre-treated using heat mediated Storage antigen retrieval with sodium citrate buffer Store at -20 °C.Stable for 12 months from (pH 6.0). The section was then incubated with date of receipt the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the Anti-ZNF148 Antibody - Protein Information chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Name ZNF148

Synonyms ZBP89

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Function Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.

Cellular Location Nucleus.

Anti-ZNF148 Antibody - Protocols

Provided below are standard protocols that you Immunofluorescent analysis of ZNF148 may find useful for product applications. staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in • Western Blot TBS for 5-10 minutes and blocked with 3% • Blocking Peptides BSA-PBS for 30 minutes at room • Dot Blot temperature. Cells were probed with the • Immunohistochemistry primary antibody in 3% BSA-PBS and • Immunofluorescence incubated overnight at 4 °C in a hidified • Immunoprecipitation chamber. Cells were washed with PBST and • Flow Cytomety incubated with a DyLight 594-conjugated • Cell Culture secondary antibody (red) in PBS at room temperature in the dark.

Anti-ZNF148 Antibody - Background

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ZNF148. The exact sequence is proprietary.

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