Nuclear Rdna ITS Sequence Data Used to Construct the First Phylogeny
Plant Syst. Evol. 230: 173±187 12002) Nuclear rDNA ITS sequence data used to construct the ®rst phylogeny of Vanguerieae Rubiaceae) H.Lantz 1, K.Andreasen 2,3, and B.Bremer 1 1Department of Systematic Botany, EBC, Uppsala University, Uppsala, Sweden 2Jepson Herbarium and Department of Integrative Biology, University of California, Berkeley, USA 3Present address: Laboratory of Molecular Systematics, Swedish Museum of Natural History, Stockholm, Sweden Received March 14, 2001 Accepted August 1, 2001 Abstract. The morphologically homogenous tribe ceae, a family of mostly woody species distrib- Vanguerieae was investigated phylogenetically us- uted over large parts of the world, but centred ing sequence data from the Internal Transcribed in the tropics. Rubiaceae are perhaps best Spacer 1ITS) region in the nuclear ribosomal DNA. known for coee 1Coea sp.), but also include Sequences from 41 Vanguerieae species represent- many ornamentals and are an important ing 19 genera were produced, and a parsimony constituent of many tropical habitats. The analysis was performed. The phylogenetic analysis main characteristics of the family are opposite has several clades with strong support, among which three new informal groups are discussed, i.e. leaves, interpetiolar stipules, sympetalous co- the Vangueria group, the Fadogia-Rytigynia group rollas and inferior ovaries. While many phy- and the Spiny group. Also found monophyletic logenies for the family have been produced in with strong support are Multidentia, Keetia, Lagy- the last ten years, all from the subfamilial level nias, and Pyrostria. Canthium and Rytigynia are down to genera, no phylogeny has so far been revealed as polyphyletic; Vangueria, Tapiphyllum, produced for Vanguerieae.
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