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Cold Spring Harbor Symposia on Quantitative Biology COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY VOLUME XLVI PART 1 COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY VOLUME XLVI ORGANIZATION OF THE CYTOPLASM COLD SPRING HARBOR LABORATORY 1982 COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY VOLUME XLVI 1982 by The Cold Spring Harbor Laboratory International Standard Book Number 0-87969-045-3 International Standard Serial Number 0091-7451 Library of Congress Catalog Card Number 34-8174 Printed in the United States of America All rights reserved COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY Founded in 1933 by REGINALD G. HARRIS Director of the Biological Laboratory 1924 to 1936 Previous Symposia Volumes I (1933) Surface Phenomena XXIII (1958) Exchange of Genetic Material: Mechanism and II (1934) Aspects of Growth Consequences III (1935) Photochemical Reactions XXIV (1959) Genetics and Twentieth Century Darwinism IV (1936) Excitation Phenomena XXV (1960) Biological Clocks V (1937) Internal Secretions XXVI (1961) Cellular Regulatory Mechanisms VI (1938) Protein Chemistry XXVII (1962) Basic Mechanisms in Animal Virus Biology VII (1939) Biological Oxidations XXVIII (1963) Synthesis and Structure of Macromolecules VIII (1940) Permeability and the Nature of Cell Membranes XXIX (1964) Human Genetics IX (1941) Genes and Chromosomes: Structure and Organi- XXX (1965) Sensory Receptors zation XXXI (1966) The Genetic Code X (1942) The Relation of Hormones to Development XXXII (1967) Antibodies XI (1946) Heredity and Variation in Microorganisms XXXIII (1968) Replication of DNA in Microorganisms XII (1947) Nucleic Acids and Nucleoproteins XXXIV (1969) The Mechanism of Protein Synthesis XIII (1948) Biological Applications of Tracer Elements XXXV (1970) Transcription of Genetic Material XIV (1949) Amino Acids and Proteins XXXVI (1971) Structure and Function of Proteins at the Three- XV (1950) Origin and Evolution of Man dimensional !Level XVI (1951) Genes and Mutations XXXVII (1972) The Mechanism of Muscle Contraction XVII (1952) The Neuron XXXVIII (1973) Chromosome Structure and Function XVIII (1953) Viruses XXXIX (1974) Tumor Viruses XIX (1954) The Mammalian Fetus: Physiological Aspects of XL (1975) The Synapse Development XLI (1976) Origins of Lymphocyte Diversity XX (1955) Population Genetics: The Nature and Causes of XLII (1977) Chromatin Genetic Variability in Population XLIII (1978) DNA: Replication and Recombination XXI (1956) Genetic Mechanisms: Structure and Function XLIV (1979) Viral Oncogenes XXII (1957) Population Studies: Animal Ecology and De- XLV (1980) Movable Genetic Elements mography All Cold Spring Harbor Laboratory publications are available through booksellers or may be ordered directly from Cold Spring Harbor Laboratory, Box 100, Cold Spring Harbor, New York 11724. SAN 203-6185 Symposium Participants ADELSTEIN, ROBERT, NHLBS, National Institutes of BRIER, JONATHAN, The Biological Laboratories, Har- Health, Bethesda, Maryland vard University, Cambridge, Massachusetts ALBRECHT-BUEHLER, GUENTER, Cold Spring Harbor BRINKLEY, BILL R., Dept. of Cell Biology, Baylor Col- Laboratory, New York lege of Medicine, Houston, Texas ALLEN, ROBERT D., Dept. of Biology, Dartmouth Col- BROWN, MICHAEL, Dept. of Molecular Genetics, Uni- lege, Hanover, New Hampshire versity of Texas Health Science Center, Dallas AMATO, PHILIP, The Biological Laboratories, Harvard BURRIDGE, KEITH, Dept. of Cell Biology, University University, Cambridge, Massachusetts of North Carolina, Chapel Hill ASANO, AKIRA, Dept. of Protein Research, Osaka Uni- CAPUT, DANIEL, Dept. of Biophysics, University of versity, Japan California, San Francisco AVlLA DE GRADO, JESUS, Dept. of Molecular Biology, CARLEY, WILLIAM W., Dept. of Applied Engineering University of Madrid, Spain Physics, Cornell University, Ithaca, New York BADER, ARTRICE, NIGMS, National Institutes of Health, CELLS, JULIO E., Chemistry Institute, Aarhus Univer- Bethesda, Maryland sity, Denmark BADER, JOHN, NCI, National Institutes of Health, Be- CHAFOULEAS, JAMES, Dept. of Cell Biology, Baylor thesda, Maryland College of Medicine, Houston, Texas BAJER, ANDREW, Dept. of Biology, University of Or- CHALFIE, MARTIN, Laboratory of Molecular Biology, egon, Eugene Medical Research Council, Cambridge, England BALCH, W. E., Dept. of Biochemistry, Stanford Uni- CHEN, LAN BO, Sidney Farber Cancer Institute, Harvard versity, California University, Boston, Massachusetts BEN ZE'EV, AVRI, Dept. of Genetics, Weizmann In- CHEUNG, WAI YIU, Dept. of Biochemistry, St. Jude stitute of Science, Rehovot, Israel Children's Research Hospital, Memphis, Tennes- BENNETT, VANN, Dept. of Anatomy, Johns Hopkins see School of Medicine, Baltimore, Maryland CHILD, FRANK, Dept. of Biology, Trinity College, BERNAL, SAM, Sidney Farber Cancer Institute, Harvard Hartford, Connecticut University, Boston, Massachusetts CHOO, QuI-LIM, Dept. of Biochemistry, University of BIRCHMEIER, WALTER, Dept. of Biochemistry, Eidge- California, San Francisco n6ssische Technische Hochschule, Ztirich, Swit- CHOW, LOUISE, Cold Spring Harbor Laboratory, New zerland York BLACK, MARK M., Dept. of Anatomy, Temple Uni- CLARKE, MARGARET, Dept. of Molecular Biology, Al- versity Medical School, Philadelphia, Pennsylvania bert Einstein College of Medicine, Bronx, New BLOBEL, GONTER, Laboratory of Cell Biology, Rock- York efeller University, New York, New York CLEGG, JAMES S., Dept. of Biology, University of BLOODGOOD, ROBERT, Dept. of Anatomy, University Miami, Coral Gables, Florida of Virginia School of Medicine, Charlottesville CLEVELAND, DON, Dept. of Biochemistry, University BLOSE, STEPHEN, Cold Spring Harbor Laboratory, New of California, San Francisco York Bomsv, GARY G., Dept. of Zoology, University of COLMAN, DAVID, Dept. of Cell Biology, New York Wisconsin, Madison University Medical Center, New York BRADY, SCOTT, Dept. of Anatomy, Case Western Re- CONDEELIS, JOHN, Dept. of Anatomy, Albert Einstein serve University, Cleveland, Ohio College of Medicine, Bronx, New York BRAELL, WILLIAM, Dept. of Biology, Massachusetts CRAIG, SUSAN, Dept. of Physiological Chemistry, Johns Institute of Technology, Cambridge Hopkins University School of Medicine, Baltimore, BRANTON, DANIEL, The Biological Laboratories, Har- Maryland vard University, Cambridge, Massachusetts CREUTZ, CARL, NIAMDD, National Institutes of Health, BRENNER, SARI, Dept. of Cell Biology, Baylor College Bethesda, Maryland of Medicine, Houston, Texas DALEY, ROGER, Dept. of Anatomical Science, State BRETScHER, ANTHONY, Dept. of Cell Biology, Uni- University of New York, Buffalo versity of Texas Health Science Center, Dallas DAVIDSON, NORMAN, Dept. of Chemistry, California BRETScHER, MARK, Laboratory for Molecular Biol- Institute of Technology, Pasadena ogy, Medical Research Council, Cambridge, DAVIS, JONATHAN, Dept. of Anatomy, Johns Hopkins England School of Medicine, Baltimore, Maryland vi SYMPOSIUM PARTICIPANTS DE BRABANDER, MARC, Dept. of Oncology, Janssen HELFMAN, DAVID, Dept. of Pharmacology, Emory Pharmaceutical Research Laboratory, Beerse, University, Atlanta, Georgia Belgium HERTZBER6, ELLIOT L., Dept. of Biology, Rockefeller DENNIS, MICHAEL, Dept. of Anatomy, University of University, New York, New York North Carolina, Chapel Hill HIGASHI-FUJIME, SUGIE, Dept. of Molecular Biology, DRAKE, PETER, Dept. of Anatomy, Case Western Re- Nagoya University, Japan serve University, Cleveland, Ohio HOLTZER, HOWARD, Dept. of Anatomy, University of DUERR, ANN, Dept. of Biology, Massachusetts Institute Pennsylvania, Philadelphia of Technology, Cambridge HOROWITZ, SAMUEL, Depts. of Molecular Pharmacology ECKERT, BARRY, Dept. of Anatomical Science, State and Cell Biology, Michigan Cancer Foundation, University of New York, Buffalo Detroit ERIKSON, RAYMOND, Dept. of Pathology, University HORWlTZ, SUSAN, Albert Einstein College of Medicine, of Colorado Health Science Center, Denver Bronx, New York FARMER, STEPHEN, Dept. of Biochemistry, Boston HUNTER, TONY, Tumor Virology Laboratory, Salk In- University, Massachusetts stitute, San Diego, California FECHHEIMER, MARCUS, The Biological Laboratories, HUXLEY, HUGH, Laboratory of Molecular Biology, Harvard University, Cambridge, Massachusetts Medical Research Council, Cambridge, England FERAMISCO, JAMES, Cold Spring Harbor Laboratory, HYNES, RICHARD, Dept. of Biology, Massachusetts In- New York stitute of Technology, Cambridge FOWLER, VELIA, NIADDK, National Institutes of ISENBERG, GERHARD, Max-Planck-Institute for Psy- Health, Bethesda, Maryland chiatry, Munich, Federal Republic of Germany FRANKE, WERNER, Dept. of Membrane Biology, Ger- JOCKUSCH, BRIGI'FI'E, European Molecular Biology Lab- man Cancer Center, Heidelberg, Federal Republic oratory, Heidelberg, Federal Republic of Germany of Germany KAMIYA, NOBURO, Dept. of Cell Biology, National In- FULTON, ALICE, Dept. of Biology, Massachusetts In- stitute for Basic Biology, Okazabi, Japan stitute of Technology, Cambridge KAULFUS, PHYLLIS, Max-Planck-Institut ffir Biophys- GARRELS, JAMES, Cold Spring Harbor Laboratory, New ishe Chemic, G6ttingen, Federal Republic of Ger- York many GEIGER, BENJAMIN, Dept. of Chemical Immunology, KEIT., CHARLES, Dept. of Pharmacology, New York Weizmann Institute of Science, Rehovot, Israel University, New York GERACE, LARRY, Dept. of Cell Biology, Johns Hopkins KELLER, THOMAS, Dept. of Biology, Yale University, School of Medicine, Baltimore, Maryland New Haven, Connecticut GLENNEY, JOHN R., JR., Max-Planck-Institut for Bio- KENDRICK-JONES, JOHN, Laboratory of Molecular Bi- physishe Chemic, G6ttingen, Federal Republic of ology,
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