Use of Living and Dying Mulches As Barriers to Protect Zucchini from Insect-Caused Viruses and Phytotoxemias
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Plant Disease Jan. 2008 PD-36 Use of Living and Dying Mulches as Barriers To Protect Zucchini from Insect-Caused Viruses and Phytotoxemias Cerruti R2 Hooks and Mark G. Wright Department of Plant and Environmental Protection Sciences Summary Infected plants are stunted, yield fewer fruits than healthy A field study was conducted to examine the influence plants, and the fruits are frequently distorted, rendering of interplanting zucchini (Cucurbita pepo L.) with the them unmarketable. In Hawai‘i, cucurbit crop losses cover crops white clover (Trifolium repens L.) and buck- due to aphid-transmitted viruses sometimes approach wheat (Fagopyrum esculentum) on densities of aphids 100 percent. and whiteflies, occurrences of aphid-caused viruses and In addition to aphids, whiteflies can be severe pests of whitefly-induced phytotoxemia, and crop yield. The cucurbit crops. Some can induce phytotoxemias in several white clover and buckwheat cover crops were used as plant species. Phytotoxemia is an adverse, often delayed a living and dying mulch, respectively. Zucchini plants reaction of plants to toxins introduced during insect feed- grown in bare-ground plots had greater aphid numbers ing. The silverleaf whitefly Bemisia( argentifolii Bellows and higher incidences of virus-infected plants than and Perring) is a severe pest of several agricultural crops those in the white clover and buckwheat treatment plots. throughout the world. Silverleaf whiteflies are responsible Whitefly nymph numbers were similar among treatment for a phytotoxemia know as squash silverleaf disorder types. However, the severity of squash silverleaf disorder (SSL). Feeding by immature silverleaf whiteflies causes was significantly lower on zucchini plants in white clover this physiological disorder, and symptom severity is treatments than in buckwheat treatments on each sam- dependant on the number of immature whiteflies per pling date. Marketable fruit yields were also significantly unit of leaf area. If populations become high enough to greater in the mulch treatments than in bare-ground plots. cause significant leaf silvering, there will be a reduction The feasibility of using white clover and buckwheat as in photosynthesis, resulting in smaller plants and severe barrier plants is discussed. yield losses in squash fields. In addition to causing SSL, whitefly damage generally includes a decline in plant Introduction vigor, irregular fruit ripening (such as is seen in tomatoes), Cucurbitaceous crops, such as squash and cucumber, are and transmission of plant viruses. susceptible to several insect-caused viruses and physi- Current control strategies for aphids and whiteflies rely ological plant disorders. Non-persistent viruses (NPVs), mostly on insecticide inputs aimed at killing them before such as zucchini yellow mosaic virus (ZYMV), transmit- they damage a crop. However, the use of insecticides may ted by several aphid species, are regarded as the most not reliably control the spread of an insect-transmitted destructive pathogens affecting cucurbits throughout the virus, especially non-persistent viruses. Non-persistent world. Among aphids, the melon aphid (Aphis gossypii viruses are transmitted non-specifically by a large num- Glover), a worldwide pest on field crops, is one of the most ber of aphid species after very brief feeding probes (1–2 efficient transmitters of plant viruses. Other important minutes or less). Integrating cultural-control techniques, aphid-transmitted viruses of cucurbits include cucumber such as mixed cropping or the growing of two or more mosaic virus (CMV), watermelon mosaic virus (WMV), plant species within the same field, with other pest-sup- and papaya ringspot virus, watermelon strain (PRSV-w). pression methods has the potential to reduce insect pest Published by the College of Tropical Agriculture and Human Resources (CTAHR) and issued in furtherance of Cooperative Extension work, Acts of May 8 and June 30, 1914, in coopera- tion with the U.S. Department of Agriculture. Andrew G. Hashimoto, Director/Dean, Cooperative Extension Service/CTAHR, University of Hawai‘i at Mänoa, Honolulu, Hawai‘i 96822. An equal opportunity/affirmative action institution providing programs and services to the people of Hawai‘i without regard to race, sex, age, religion, color, national origin, ancestry, dis- ability, marital status, arrest and court record, sexual orientation, or status as a covered veteran. CTAHR publications can be found on the Web site <http://www.ctahr.hawaii.edu/freepubs>. UH–CTAHR Mulch Barriers To Protect Zucchini PD-36 — Jan. 2008 numbers and the resulting transmission of viruses and of zucchini 1.5 m (5 ft) apart with plants spaced 1.2 m phytotoxemias. (4 ft) apart within them. Alleyways between plots were a Using zucchini as a model cucurbit system, this minimum of 7 m wide and kept free of weeds. Bordering study investigated the value of mixed-cropping systems the study sites were rows of Sudax (a sorghum–sudan (specifically, use of living and dying mulch) for reduc- grass hybrid), which were sprayed weekly with GF 120 ing aphid and whitefly populations and their associated Naturalyte fruit fly bait to help manage populations of plant impairments (specifically, non-persistent viruses the melon fly, Dacus cucurbitae Coquillett. and squash silverleaf disorder). Dying mulches are cover crops, grown within a main crop, that will began Insect counts to senesce and eventually die prior to completion of the Counts of adult whiteflies and aphids on zucchini leaves main crop harvest period. The dying mulch’s purpose is were taken at weekly intervals beginning 7 days after to serve some benefit prior to dying, such as increasing planting (DAP) and continuing until 49 DAP. A total of the numbers of beneficial organisms, improving soil 16 plants were randomly selected from each plot during fertility, or suppressing nematodes. Living mulches are a sampling period by gently turning over one randomly cover crops interplanted or undersown with a main crop selected leaf from each plant and recording the number that will continue to grow and mature along with the of insects found. Because the alate (winged) aphids are main crop; they are intended to serve a function such as responsible for virus spread, only this aphid morph was weed or insect suppression. Barrier plants are secondary counted. In addition, aphids (winged and wingless) and plants grown within or bordering a primary cash crop for immature stages (eggs, nymphs, and pupae) of whiteflies the purpose of controlling insect-caused plant diseases. were sampled by taking a 3.14 cm2 disc sample from the This paper describes a field experiment designed to use foliage of 12 randomly selected zucchini plants per plot both living and dying mulches as barrier plants to reduce using a cork bore sampler. The disc samples were then occurrence of NPVs and SSL disorder. taken to a laboratory and examined under a microscope. The numbers of immature whiteflies and aphids found Materials and methods on these discs were recorded. Experiment layout Disease and phytotoxemia rating A field experiment was conducted at the University of The percentage of zucchini plants with a non-persistent Hawai‘i at Mānoa Poamoho Research Station on the virus was determined by visually inspecting all zucchini island of O‘ahu in 2003. The three cropping habitats plants in each plot. The percentage of plants showing examined during this study were zucchini plants under- viral symptoms (e.g., mosaic leaves, distorted and/or sown either in (1) a living mulch, white clover var. New mottled fruit) was recorded weekly. Silverleaf disorder Zealand (Trifolium repens L.), seeded at ~54 g per row; was rated on the new leaf growth of all plants in three (2) a dying mulch, buckwheat (Fagopyrum esculentum randomly selected interior rows of each plot. A scale Moench, Peaceful Valley Farm Supply, Grass Valley, Ca- of 0 to 5 was used during each inspection date, with 0 lif.), broadcast-seeded at a rate of ~1.81 kg (4 lb) per plot; indicating no symptoms and 5 indicating that the entire or (3) grown in bare-ground monoculture (the “control” leaf contained a silver coloring. Plants were evaluated treatment). Experimental plots were 13.7 m x 13.7 m (45 for viral symptoms every five days beginning 30 DAP, ft x 45 ft), with each treatment replicated four times and and silverleaf severity symptoms were assessed every 10 arranged in a randomized complete block design. days beginning 16 DAP. White clover was sown on March 31, 2003 and buck- wheat on September 18, 2003. On September 24, a weed Statistical analysis string-trimmer was used to clear eight 76-cm (2.5-ft) rows The effects of mulch type on each experimental factor in the white clover plots. Afterward, a motorized hand were analyzed using analysis of variance (Proc GLM, tiller was used to cultivate the rows. On October 13, 2- SAS Institute) and predetermined orthogonal compari- week-old greenhouse-grown zucchini plants (‘Spineless sons to separate mean differences. Within the model, Beauty’, Syngenta Seed, Boise, Idaho) were transplanted the following predetermined statistical contrasts were into each treatment plot. Each plot contained eight rows conducted: zucchini monoculture vs. mulches (buck- 2 UH–CTAHR Mulch Barriers To Protect Zucchini PD-36 — Jan. 2008 Figure 1. Mean population densities of winged aphids in bare-ground (zucchini monoculture), buckwheat (zucchini- buckwheat), and white clover (zucchini-white clover). 3.0 Bare-ground * 2.7 Buckwheat White clover 2.4 2.1 1.8 1.5 1.2 * b 0.9 0.6 * Number of winged aphids per leaf * No. of winged0.3 aphids* per leaf 0.0 14 21 28 35 42 49 Days after planting Days after Planting * indicates average numbers in bare-ground were significantly greater than in buckwheat + white clover. b indicates average numbers in buckwheat were significantly greater than in white clover (P < 0.05). wheat and white clover), and buckwheat vs. white clover. counts significantly higher in buckwheat than in white Treatment comparisons were considered significantly clover plots.