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HISTOCHEMICAL INVESTIGATION of TWO MEDICINAL PLANTS in MAHARASHTRA Kadam V.B.*, Tambe S.S.1, Sumia Fatima2, Momin R.K.3 * P.G
Kadam et al Journal of Drug Delivery & Therapeutics; 2013, 3(5), 62-64 1 Available online at http://jddtonline.info RESEARCH ARTICLE HISTOCHEMICAL INVESTIGATION OF TWO MEDICINAL PLANTS IN MAHARASHTRA Kadam V.B.*, Tambe S.S.1, Sumia Fatima2, Momin R.K.3 * P.G. Department of Botany & Research Centre, K.T.H.M. College, Nasi, India- 422002 1 Department of Botany, P.H. Mahila College, Malegaon (Nashik), India- 432 003 2 Department of Botany, Dr. Rafiq Zakaria College for women. Aurangabad, India- 431001 3Department of Botany, Milliya Arts, Science and Management Science College, Beed, India *Corresponding author- Email ID: [email protected] ABSTRACT The histochemical studies of leaves and wood of Butea monosperma Lam and Madhucaindica Gmel are medicinal important plants in Maharashtra. For histochemical studies the free hand sections of leaves and wood were taken and treated with the respective reagent in localize components, viz. starch, protein, tannin, saponin, fat, glucosides and alkaloids in the tissues. Key words: Histochemistry, starch, protein, tannin, saponin, fat, glucosides and alkaloids INTRODUCTION Among ancient civilizations, India has been known to be and treated the respective reagent to localize component , rich repository of medicinal plants. The forest in India is Viz. starch, protein, tannin, saponin, fat, glucosides and the principal repository of large number of medicinal and alkaloids in the tissues 7. aromatic plants, which are largely collected as raw 1) Starch - 0.3 g of iodine and 1.5 g of potassium iodide materials for manufacture of drugs and perfumery were dissolved in 100 ml of distilled water. A drop of the products. -
บทที่ 5 เซลล์ของพืชที่ให้เนื้อไม้ (Woody Plant Cells)
บทที่ 5 เซลล์ของพืชที่ให้เนื้อไม้ (Woody Plant Cells) เนื้อไม้ คือ กลุ่มของหน่วยเล็กๆ จ ำนวนนับไม่ถ้วน แต่ละหน่วย เรียกว่ำ เซลล์ แต่ละเซลล์เชื่อมติดกันโดยชั้นของสำรที่เรียกว่ำ intercellular substance แต่ละเซลล์มีผนังที่จ ำกัดซึ่งมีช่องว่ำง อยู่ ภำยในบรรจุสำรที่ส ำคัญและท ำให้เซลล์ด ำรงชีวิตอยู่ได้ คือ protoplasm เซลล์หลำยเซลล์รวมกันเรียกว่ำ เนื้อเยื่อ (tissue) https://cellsells.wordpress.com/2015/06/05/ cell-organelles/#jp-carousel-106 ลักษณะของเซลล์พืช ต้นพืชประกอบด้วยส่วนประกอบที่มีลักษณะเป็นห้องๆ คล้ำยกล่องขนำดเล็ก เรียกว่ำ เซลล์ (cell) เซลล์เป็น ส่วนประกอบพื้นฐำนของสิ่งมีชีวิต เซลล์พืชถูกล้อมรอบด้วย ผนังเซลล์ (cell wall) ซึ่งภำยในประกอบด้วยส่วนประกอบทำง เคมีที่ซับซ้อน เซลล์ที่ปรำศจำกส่วนประกอบทำงเคมีเหล่ำนี้จะ เป็นเซลล์ที่ตำยแล้ว ส่วนประกอบภำยในเซลล์ สิ่งที่อยู่ภำยในเซลล์พืชท้ังหมด คือ protoplast ซึ่งประกอบด้วย ส่วนประกอบ 2 กลุ่มใหญ่ๆ คือ 1.กลุ่มที่เป็ นส่วนประกอบที่มีชีวิต เช่น cytoplasm และ organelles ต่ำงๆ รวมทั้ง nuscleus, plastids, mitrochondria, Golgi bodies, sphaerosomes, lysosomes, ribosomes, endophasmic reticulum และ microtubules 2.กลุ่มที่เป็ นส่วนประกอบที่ไม่มีชีวิต ซึ่งถูกล้อมรอบด้วย cytoplasm เช่น vacuoles และ ergastic substances ซึ่งรวมถึงสำรที่ สะสมไว้ เช่น เม็ดแป้ง (starch grain) หยดน ้ำมัน (oil droplets) และ ผลิตผลจำกขบวนกำร metabolism อื่นๆ เช่น ผลึกชนิดต่ำงๆ https://cellsells.wordpress.com/2015/06/05/ cell-organelles/#jp-carousel-106 Protoplasm Protoplasm เป็ นมวลสำรพื้นฐำนของสิ่งมีชีวิตอยู่ภำยใน เซลล์ของเซลล์ที่มีชีวิต โดยทั่วๆไป แบ่งออกเป็นส่วนส ำคัญ 2 ส่วน คือ nucleus และ cytoplasm protoplasm เป็นของเหลว ข้นๆ -
Atmospheric CO2 Concentration, N Availability, and Water Status Afect
Trees (1997) 11: 494 – 503 © Springer-Verlag 1997 ORIGINAL ARTICLE Seth Pritchard ? Curt Peterson ? G. Brett Runion Stephen Prior ? Hugo Rogers Atmospheric CO2 concentration, N availability, and water status affect patterns of ergastic substance deposition in longleaf pine (Pinus palustris Mill.) foliage Received: 5 November 1996 / Accepted: 7 March 1997 Abstract mLeaf chemistry alterations due to increasing longleaf pine, and that normal variability in leaf tissue atmospheric CO2 will reflect plant physiological changes quality resulting from gradients in soil resources will be and impact ecosystem function. Longleaf pine was grown magnified under conditions of elevated CO2. for 20 months at two levels of atmospheric CO2 (720 and 365 µmol mol–1), two levels of soil N (4 g m–2 year–1 and Key wordsm Pinus palustris ? Pinaceae ? Elevated CO2 ? 40 g m–2 year–1), and two soil moisture levels (– 0.5 and Ergastic substances –1.5 MPa) in open top chambers. After 20 months of exposure, needles were collected and ergastic substances including starch grains and polyphenols were assessed using light microscopy, and calcium oxalate crystals were Introduction assessed using light microscopy, scanning electron micros copy, and transmission electron microscopy. Polyphenol Atmospheric CO2 is increasing; a recent estimate predicts content was also determined using the Folin-Denis assay that current levels will double within the next century and condensed tannins were estimated by precipitation with (Keeling et al. 1989). The effects of elevated CO2 on protein. Evaluation of phenolic content histochemically was plant and ecosystem processes are currently receiving a compared to results obtained using the Folin-Denis assay. -
Department of Physics Determination of Dielectric
DEPARTMENT OF PHYSICS DETERMINATION OF DIELECTRIC CONSTANT Karthika.S, Kavipriya.S, Keerthana.M, Manjumalini.K.C, Mythili.N Supervisor: Dr. Mrs. P. Meena ABSTRACT A dielectric is an electric insulator that can be polarized by an applied electric field. When a dielectric is placed in an electric field, electric charges do not flow through the material as they do in an electric conductor, but only slightly shift from their average equilibrium positions causing dielectric polarization. A number of traditional dielectric materials are widely used in industry. Typical examples are paper, glass, rubber, vacuum, polymers, gases, liquids, mica, ceramics. The dielectric constant is the ratio of the permittivity of a substance to the permittivity of free space. It is an expression of the extent to which a material concentrates electric flux, and is the electrical equivalent of relative magnetic permeability. The experimental setup includes electrodes like aluminum, tin and iron, and dielectric materials like rubber, cardboard and glass and the LCR Bridge (12 kHz-100 kHz). The values of the capacitance and dielectric constant for different combination of electrodes and with different dielectrics were observed. P 1 A major use of dielectrics is in fabricating capacitors. These have many uses including storage of energy in the electric field between the plates, filtering out noise from signals as part of a resonant circuit, and supplying a burst of power to another component. RESULT The dielectric constants of Glass, Cardboard, and Rubber were determined with electrodes made of Tin, Aluminium, and Iron using the LCR bridge. It is observed that the dielectric material Glass with the electrodes of Tin has the highest Dielectric constant of 0.9901(approximately equal to 1) at a frequency of 10 kHz. -
Vegetative Anatomy of the Haemodoraceae and Its Phylogenetic Significance
Int. J. Plant Sci. 178(2):117–156. 2017. q 2016 by The University of Chicago. All rights reserved. 1058-5893/2017/17802-0004$15.00 DOI: 10.1086/689199 VEGETATIVE ANATOMY OF THE HAEMODORACEAE AND ITS PHYLOGENETIC SIGNIFICANCE Layla Aerne-Hains1,* and Michael G. Simpson2,* *Department of Biology, San Diego State University, San Diego, California 92182, USA Editor: Félix Forest Premise of research. Haemodoraceae are a relatively small monocot family consisting of 14 genera and approximately 108 species and are distributed in parts of Australia, southern Africa, South and Central America, and eastern North America. The family is divided into two subfamilies, Haemodoroideae and Conostylidoideae. This research focuses on the vegetative anatomy of the family, with an emphasis on leaf anatomical features. The aims of this project are (1) to acquire new vegetative anatomical data for a large selection of Haemodoraceae and (2) to evaluate these data in the context of both phylogenetic relationships and environmental factors. Methodology. Cross sections of roots, stems (scapes), or leaves of 60 species and 63 ranked taxa from all 14 genera of the family were prepared and stained using standard histological methods, and SEMs were made of the leaf surface. Line drawings were prepared of leaf cross sections of an exemplar of each genus. Tissues and cells were examined and photographed, and comparisons were made among taxa. For leaf epidermal cells, the ratio of cell wall transectional area∶cell transectional area was calculated and plotted. Several dis- crete anatomical characters and character states were defined and plotted on a recently derived cladogram and examined for phylogenetic signal. -
Full Article-PDF
Kadam V.B et al, IJPNM, 2014, Vol.2(2): 140-145 ISSN: 2321-6743 Research Article International Journal of Pharmacy and Natural Medicines www.pharmaresearchlibrary.com/ijpnm Histochemical Investigation of Some Medicinal plants of genus Terminalia of (Combretaceae) in Maharashtra Kadam V.B*1, Sunanda Salve1 and K.R. Khandare2 1P.G. Department of Botany & Research Centre, K.T.H.M. College, Nasik – 422002 2Department of Botany, N.M. Sonawane Science College , Satana, Nasik Received: 20 July 2014, Accepted: 21 October 2014, Published Online: 15 December 2014 Abstract The histochemical studies of leaves and wood of Terminalia cuneata Terminalia bellerica, Terminalia chebula and Terminalia catappa are medicinally important plants in Maharashtra. For histochemical studies the free hand sections of leaves and wood were taken and treated with the respective reagent in localize components, viz. starch, protein, tannin, saponin, fat, glucosides and alkaloids in the tissues. Keywords: Histochemistry, starch, protein, tannin, saponin, fat, glucosides and alkaloids. Contents 1. Introduction . 140 2. Materials and Method. .141 3. Results and Discussion. .142 4. References . .145 *Corresponding author Kadam V.B P.G. Department of Botany & Research Centre, K.T.H.M. College, Nasik – 422002 Manuscript ID: IJPNM2209 PAPER-QR CODE Copyright © 2014, IJPNM All Rights Reserved 1. Introduction Among ancient civilizations, India has been known to be rich repository of medicinal plants. The forest in India is the principal repository of large number of medicinal and aromatic plants, which are largely collected as raw materials for manufacture of drugs and perfumery products. The knowledge about the use of medicinal plants has been acquired through centuries and such plants are still valued even today. -
Histochemical Investigation of Different Organs of Holy Plants from Marathwada Region(India)
HISTOCHEMICAL INVESTIGATION OF DIFFERENT ORGANS OF HOLY PLANTS FROM MARATHWADA REGION(INDIA) A.N Dharasurkar Department of Botany, Vasant Dada Patil College, Patoda, Dist- Beed. Maharashtra, India Abstract The histochemical studies of leaves and Stem of holy & medicinally important plants of Marathwada. For histochemical studies the free hand sections of leaves and Stems were taken and treated with the respective reagent in localize components, viz. starch, protein, tannin, saponin, fat, glucosides and alkaloids in the tissues. Keywords: Histochemistry , starch , protein , tannin , saponin , fat , glucosides and alkaloids. INTRODUCTION Marathwada is a rich source of plant and animal wealth, which is due to its varied geographical and agro-climatic regions. Besides it's varied biodiversity, it has a diverse cultural heritage too. Though at present Marathwada health care delivery consists of both traditional and modem systems of medicines, both organized traditional systems of medicine like Ayurveda, Siddha and Unani and unorganized systems like folk medicine have been flourishing well. Ayurveda and Siddha are of Indian origin and accounted for about 60% health care delivery in general and 75% of rural Indian population depends on these traditional systems. These two systems of medicine use plants, minerals, metals and animals as source of drugs, plants being the major source. It is estimated that roughly 1500 plant species in Ayurveda and 1200 plant species in Siddha have been used for drug preparation (Jain, 1987, Krishnakumar and Sureshkumar, 1995). In Indian folk medicine use, about 7500 plant species are recorded as medicinal plants (Anonymous, 1996). Many plants contain medicinally important secondary product (Dhar et al.,1968). -
Eukaryotic Cell Organelles
Department of Genetics and Plant Breeding Ch. Charan Singh University, Meerut Plant Physiology: An Open Elective Course (Study Material) Cell physiology: Cell organelles and their physiological functions, structure and physiological functions of cell wall, cell inclusions, cell membrane structure and functions. ------(syllabus of Unit 1) Suggested Readings (i) Salisbury FB and Ross, CW (1986) Plant Physiology, CBS Publishers & Distributors, New Delhi. (ii) Taize L and Zeiger E (2006) Plant Physiology. Sinauer Associates, Inc, Publishers, Sunderland, Massachusetts, USA. (iii) Hopkins WG and Huner NPA (2004) Introduction to Plant Physiology. John Wiley & Sons. (iv) Oxlade Edwin (2010) Plant Physiology: The Structure of Plants Explained. In-focus: Studymates. (v) Lodish, H, et al. "The Dynamic Plant Cell Wall." Molecular Cell Biology. 4th ed., W. H. Freeman, 2000, www.ncbi.nlm.nih.gov/books/NBK21709/. (vi) Young, Kevin D. “Bacterial Cell Wall.” Wiley Online Library, Wiley/Blackwell (10.1111), 19 Apr. 2010, onlinelibrary.wiley.com/doi/abs/10.1002/9780470015902.a0000297.pub2. Eukaryotic Cell Organelles Eukaryotic cells are structurally complex, and by definition are organized, in part, by interior compartments that are themselves enclosed by lipid membranes that resemble the outermost cell membrane. The larger organelles, such as the nucleus and vacuoles, are easily visible with the light microscope. They were among the first biological discoveries made after the invention of the microscope. Not all eukaryotic cells have each of the organelles listed below. Exceptional organisms have cells that do not include some organelles that might otherwise be considered universal to eukaryotes (such as mitochondria).[18] There are also occasional exceptions to the number of membranes surrounding organelles, listed in the tables below (e.g., some that are listed as double-membrane are sometimes found with single or triple membranes). -
World Journal of Pharmaceutical Research Kadam Et Al
World Journal of Pharmaceutical Research Kadam et al . World Journal of Pharmaceutical SJIF ImpactResearch Factor 7.523 Volume 6, Issue 3, 1008-1015. Research Article ISSN 2277– 7105 HISTOCHEMICAL INVESTIGATION OF CASSIA TORA LINN. Gaykhe Rahul C. and *Kadam Vasant B. P.G. Department of Botany & Research Centre, K.T.H.M. College, Nasik – 422002. Article Received on ABSTRACT 05 Jan. 2017, Cassia tora Linn. (Caesalpinaceae) is a semi wild annual herb grown Revised on 26 Jan. 2017, Accepted on 16 Feb. 2017 widely in different places of India. This plant species is well known for DOI: 10.20959/wjpr20173-7954 having potential in traditional medicine practices for the treatment of a variety of disorders and ailments ranging from cough, hypertension, *Corresponding Author antimicrobial, antioxidant antidiabetic. This paper encompasses a Dr. Kadam Vasant B. comprehensive review on histochemical and biological aspects of P.G. Department of Botany Cassia tora Linn. For histochemical studies the freehand sections of & Research Centre, leaves, stem and roots were taken and treated with the respective K.T.H.M. College, Nasik – 422002. reagent in localize components, viz. starch, protein, tannin, saponin, fat, glucosides and alkaloids in the tissues. KEYWORDS: Histochemistry, starch, protein, tannin, saponin, fat, glucosides and alkaloids. INTRODUCTION Nature has provided the storehouse of remedies to cure all ailments of mankind.The traditional herbal medicines are still practiced in large part of our country mostly in tribal and rural areas. In many developing countries large section of population relies on traditional practioners, who are dependent on herbal folk medicines for their primary health care and has deep faith in it. -
FOREST BOTANY Part - I
FOREST BOTANY Part - I DIRECTORATE OF FORESTS GOVERNMENT OF WEST BENGAL FOREST BOTANY PART - I 1 This edition is published by Development Circle, Directorate of Forests, Government of West Bengal, 2016 Aranya Bhavan LA – 10A Block, Sector III Salt Lake City, Kolkata, West Bengal, 700098 Copyright © 2016 in text Copyright © 2016 in design and graphics All rights reserved. No part of this publication may be reproduced, stored in any retrieval system or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior written permission of the copyright holders. 2 FOREST BOTANY PART - I PREFACE Botany is one of the core subjects of forestry. Scientific management of plant resources of forests requires a forest manager to familiarize himself with the fundamentals of the plants – their internal and external structure, diverse physiological functions, interaction with the environment in which they grow, their uses and other aspects related to plant life. As part of the JICA project on ‘Capacity Development for Forest Management and Training of Personnel’ being implemented by the Forest Department, Govt of West Bengal, these course materials on Forest Botany have been prepared for induction training of the Foresters and Forest Guards. The object of this training manual is to present the basic aspects of Forest Botany. The subjects covered in these materials broadly conform to syllabus laid down in the guidelines issued by the Ministry of Environment of Forests, Govt of India, vide the Ministry’s No 3 -17/1999-RT dated 05.03.13. In dealing with some of the parts of the course though, the syllabus has undergone minor revision to facilitate better understanding of the subjects and to provide their appropriate coverage. -
Histochemical Investigation of Syzygium Cumini L (Skeels)
www.ijcrt.org © 2021 IJCRT | Volume 9, Issue 4 April 2021 | ISSN: 2320-2882 Histochemical Investigation of Syzygium cumini L (Skeels) S.S.Tambe Department of Botany, MGV’S Arts, Science and Commerce College Manmad, Nashik Abstract: The present work is under taken with a view to analyze, similarities and dissimilarities, anatomical, microscopically, physicochemical. These plants are commonly available and medicinally useful in this geographical area and this study would form a foundation for understanding the pharmacological and therapeutically effectiveness of these varieties. One of such resources is traditional medicines. Systematic screening of them may result in the discovery of Secondary metabolic compounds location and quantity in plant organs. This Research Article Histochemical investigation of Syzygium cumini these plants have many traditional medicinal used. KEY WORDS: Histochemistry, traditional Medicine, Secondary metabolites, Syzygium cumini L (Skeels) Introduction Several published reports demonstrate the use of histochemistry to locate essential oil, such as the localization of citral accumulation in Cymbopogon citratus (E. Lewinsohn etal 1982) ,where the aldehyde- specific Schiff's reagent was used to detect the monoterpene aldehydes neral and geranial (citral), and the lipid stains Sudan red and Nile blue were used to locate essential oil in leaves of Salvia aurea (G. Serrato-Valenti etal 1997) Histochemistry is the branch of histology dealing with the identification of chemical components of cells and tissues. Starch deposition occurs widely in the plant body, but the particularly common places of its accumulation are seeds, the parenchyma of the secondary vascular tissues in the stem and root, tubers, rhizomes and corn (V. B. Kadam, 1999) Starch and proteins are the principal ergastic substances of the protoplast (E. -
Comparative Vegetative Anatomy and Systematics of the Angraecoids (Vandeae, Orchidaceae) with an Emphasis on the Leafless Habit Barbara S
Eastern Illinois University The Keep Faculty Research & Creative Activity Biological Sciences January 2006 Comparative vegetative anatomy and systematics of the angraecoids (Vandeae, Orchidaceae) with an emphasis on the leafless habit Barbara S. Carlsward Eastern Illinois University, [email protected] William Louis Stern University of Florida, Gainesville Benny Bytebier University of Stellenbosch Follow this and additional works at: http://thekeep.eiu.edu/bio_fac Part of the Biology Commons Recommended Citation Carlsward, Barbara S.; Stern, William Louis; and Bytebier, Benny, "Comparative vegetative anatomy and systematics of the angraecoids (Vandeae, Orchidaceae) with an emphasis on the leafless habit" (2006). Faculty Research & Creative Activity. 259. http://thekeep.eiu.edu/bio_fac/259 This Article is brought to you for free and open access by the Biological Sciences at The Keep. It has been accepted for inclusion in Faculty Research & Creative Activity by an authorized administrator of The Keep. For more information, please contact [email protected]. Comparative vegetative anatomy and systematics of the angraecoids (Vandeae, Orchidaceae) with an emphasis on the leafless habit BARBARA S. CARLSWARD, WILLIAM LOUIS STERN, and BENNY BYTEBIER Abstract The vegetative anatomy and morphology of 142 species of the angraecoid orchids (Angraecinae + Aerangidinae) and 18 species of Aeridinae were examined using light and scanning electron microscopy. Leafless members of Vandeae were of particular interest because of their unique growth habit. Leafy and leafless members of Angraecinae and Aerangidinae were examined and compared with specimens of Aeridinae. Vandeae were homogeneous in both leaf and root anatomy. A foliar hypodermis and fibre bundles were generally absent. Stegmata with spherical silica bodies were found associated with sclerenchyma and restricted to leaves in almost all specimens examined.